Biology
- Cell culture (bacterial and eukaryotic cell lines)
- SILAC (eukaryotic and bacterial cultures)
- CRISPR/Cas9 DNA editing (eukaryotic cells)
Biochemistry
- PAGE (1D, 2D, Blue Native)
- Western blotting
- GeLC-MS
- Filter-aided sample preparation (FASP)
- Dimethyl- and TMT-labeling of peptides
- Phosphopeptide enrichment
- Strong cation exchange chromatography (peptide separation)
- high-pH (HpH) C18 peptide separation
- Stage-tip peptide purification
- Pipetting robot (Opentrons OT-2)
Liquid Chromatography - Mass Spectrometry
- NanoHPLC column packing unit, 2x (Nanobaume)
- 2D nano-HPLC (Eksigent)
- 1D nano-HPLC, 2x (Proxeon Biosystems/Thermo)
- 1D nano-UHPLC 4x (Thermo)
- Q-Exactive HF Mass Spectrometer (Thermo)
- Orbitrap Exploris 480 Mass Spectrometer (Thermo)
IT/Bioinformatics
- Bioinformatic workflow for nsSNV calling and integration into protein databases
- Bioinformatic workflow for metaproteome analysis
- Pipeline for Orbitrap data analysis (based on MaxQuant)
- Protein/Peptide identification
- SILAC quantitation
- Peptide modification analysis
- Downstream analysis of shotgun proteomics data
- 150TB local storage capacity
