Interfaculty Institute of Biochemistry (IFIB)

Group Publications

Bax retrotranslocation potentiates Bcl-xL's antiapoptotic activity and is essential for switch-like transitions between MOMP competency and resistance. Hantusch A, Das KK, García-Sáez AJ, Brunner T, Rehm M. Cell Death Dis. 2018 Mar 22;9(4):430. doi: 10.1038/s41419-018-0464-6. PMID: 29567940

The mycotoxin phomoxanthone A disturbs the form and function of the inner mitochondrial membrane. Böhler P, Stuhldreier F, Anand R, Kondadi AK, Schlütermann D, Berleth N, Deitersen J, Wallot-Hieke N, Wu W, Frank M, Niemann H, Wesbuer E, Barbian A, Luyten T, Parys JB, Weidtkamp-Peters S, Borchardt A, Reichert AS, Peña-Blanco A, García-Sáez AJ, Itskanov S, van der Bliek AM, Proksch P, Wesselborg S, Stork B. Cell Death Dis. 2018 Feb 19;9(3):286. doi: 10.1038/s41419-018-0312-8. PMID: 29459714

Bcl-2 proteins: Unraveling the details of a complex and dynamic network. Bleicken S, Garcia-Saez AJ. Mol Cell Oncol. 2017 Oct 30;5(1):e1384880. doi: 10.1080/23723556.2017.1384880. eCollection 2018. PMID: 29404386

Drp1 polymerization stabilizes curved tubular membranes similar to those of constricted mitochondria. Ugarte-Uribe B, Prévost C, Das KK, Bassereau P, García-Sáez AJ. J Cell Sci. 2018 Apr 19;132(4). pii: jcs208603. doi: 10.1242/jcs.208603. PMID: 29361534

Bax, Bak and beyond - mitochondrial performance in apoptosis. Peña-Blanco A, García-Sáez AJ. FEBS J. 2018 Feb;285(3):416-431. doi: 10.1111/febs.14186. Epub 2017 Sep 4. Review. PMID: 28755482

Dynein light chain 1 induces assembly of large Bim complexes on mitochondria that stabilize Mcl-1 and regulate apoptosis.Singh PK, Roukounakis A, Frank DO, Kirschnek S, Das KK, Neumann S, Madl J, Römer W, Zorzin C, Borner C, Haimovici A, Garcia-Saez A, Weber A, Häcker G. Genes Dev. 2017 Sep 1;31(17):1754-1769. doi: 10.1101/gad.302497.117. PMID: 28982759

Transient apoptosis inhibition in donor stem cells improves hematopoietic stem cell transplantation.Kollek M, Voigt G, Molnar C, Murad F, Bertele D, Krombholz CF, Bohler S, Labi V, Schiller S, Kunze M, Geley S, Niemeyer CM, Garcia-Saez A, Erlacher M. J Exp Med. 2017 Oct 2;214(10):2967-2983. doi: 10.1084/jem.20161721. Epub 2017 Sep 7. PMID: 28882984

Does Ceramide Form Channels? The Ceramide-Induced Membrane Permeabilization Mechanism. Artetxe I, Ugarte-Uribe B, Gil D, Valle M, Alonso A, García-Sáez AJ, Goñi FM. Biophys J. 2017 Aug 22;113(4):860-868. doi: 10.1016/j.bpj.2017.06.071. PMID: 28834722

Bax, Bak and beyond: mitochondrial performance in apoptosis. Peña-Blanco A, García-Sáez AJ. FEBS J. 2017 Jul 29. doi: 10.1111/febs.14186. [Epub ahead of print] Review.PMID:2875548

Quantitative interactome of a membrane Bcl-2 network identifies a hierarchy of complexes for apoptosis regulation.Bleicken S, Hantusch A, Das KK, Frickey T, Garcia-Saez AJ.Nat Commun. 2017 Jul 13;8(1):73. doi: 10.1038/s41467-017-00086-6.

Apoptotic foci at mitochondria: in and around Bax pores.Ugarte-Uribe B, García-Sáez AJ. Philos Trans R Soc Lond B Biol Sci. 2017 Aug 5;372(1726). pii: 20160217. doi: 10.1098/rstb.2016.0217. Review.PMID: 28630156

Improving certainty in single molecule imaging.Danial JS, García-Sáez AJ. Curr Opin Struct Biol. 2017 May 5;46:24-30. doi: 10.1016/j.sbi.2017.04.007. [Epub ahead of print] Review.PMID: 28482279

Necroptosis Execution Is Mediated by Plasma Membrane Nanopores Independent of Calcium. Ros U, Peña-Blanco A, Hänggi K, Kunzendorf U, Krautwald S, Wong WW, García-Sáez AJ. Cell Rep. 2017 Apr 4;19(1):175-187. doi: 10.1016/j.celrep.2017.03.024.

Determinants of BH3 Sequence Specificity for the Disruption of Bcl-xL/cBid Complexes in Membranes. Das KK, Shalaby R, García-Sáez AJ. ACS Chem Biol. 2017 Apr 21;12(4):989-1000. doi: 10.1021/acschembio.6b01084. Epub 2017 Feb 17. PMID: 28170214

The membrane activity of BOK involves formation of large, stable toroidal pores and is promoted by cBID. Fernández-Marrero Y, Bleicken S, Das KK, Bachmann D, Kaufmann T, Garcia-Saez AJ. FEBS J. 2017 Jan 8. doi: 10.1111/febs.14008. [Epub ahead of print] PMID:28064468

Pro-apoptotic cBid and Bax exhibit distinct membrane remodeling activities: An AFM study. Unsay JD, Cosentino K, Sporbeck K, García-Sáez AJ. Biochim Biophys Acta. 2016 Oct 15;1859(1):17-27. doi: 10.1016/j.bbamem.2016.10.007. [Epub ahead of print] PMID:27755971

Disrupting a key hydrophobic pair in the oligomerization interface of the actinoporins impairs their pore-forming activity. Mesa-Galloso H, Delgado-Magnero KH, Cabezas S, López-Castilla A, Hernández-González JE, Pedrera L, Alvarez C, Tieleman DP, García-Saez AJ, Lanio ME, Ros U, Valiente PA. Protein Sci. 2016 Dec 21. doi: 10.1002/pro.3104. [Epub ahead of print] PMID:28000294

Bax and Bak Pores: Are We Closing the Circle? Cosentino K, García-Sáez AJ. Trends Cell Biol. 2016 Dec 5. pii: S0962-8924(16)30202-1. doi: 10.1016/j.tcb.2016.11.004. [Epub ahead of print] Review.PMID:27932064

Pushing the size limit of de novo structure ensemble prediction guided by sparse SDSL-EPR restraints to 200 residues: The monomeric and homodimeric forms of BAX. Fischer AW, Bordignon E, Bleicken S, García-Sáez AJ, Jeschke G, Meiler J. J Struct Biol. 2016 Jul;195(1):62-71. doi: 10.1016/j.jsb.2016.04.014.

MOMP in the absence of BH3-only proteins. García Sáez AJ, Villunger A. Genes Dev. 2016 Apr 15;30(8):878-80. doi: 10.1101/gad.281519.116.

Quaternary structure of a G-protein-coupled receptor heterotetramer in complex with Gi and Gs. Navarro G, Cordomí A, Zelman-Femiak M, Brugarolas M, Moreno E, Aguinaga D, Perez-Benito L, Cortés A, Casadó V, Mallol J, Canela EI, Lluís C, Pardo L, García-Sáez AJ, McCormick PJ, Franco R. BMC Biol. 2016 Apr 5;14:26. doi: 10.1186/s12915-016-0247-4.

cBid, Bax and Bcl-xL exhibit opposite membrane remodeling activities. Bleicken S, Hofhaus G, Ugarte-Uribe B, Schröder R, García-Sáez AJ. Cell Death Dis. 2016 Feb 25;7:e2121. doi: 10.1038/cddis.2016.34. PMID: 26913610

Bax assembly into rings and arcs in apoptotic mitochondria is linked to membrane pores. Salvador-Gallego R, Mund M, Cosentino K, Schneider J, Unsay J, Schraermeyer U, Engelhardt J, Ries J, García-Sáez AJ. EMBO J. 2016 Jan 18. pii: e201593384. [Epub ahead of print] PMID: 26783362

Membrane Disintegration Caused by the Steroid Saponin Digitonin Is Related to the Presence of Cholesterol. Sudji IR, Subburaj Y, Frenkel N, García-Sáez AJ, Wink M. Molecules. 2015 Nov 9;20(11):20146-60. doi: 10.3390/molecules201119682. PMID:26569199 .

Bax monomers form dimer units in the membrane that further self-assemble into multiple oligomeric species. Subburaj Y, Cosentino K, Axmann M, Pedrueza-Villalmanzo E, Hermann E, Bleicken S, Spatz J, García-Sáez AJ. Nat Commun. 2015 Aug 14;6:8042. doi: 10.1038/ncomms9042.

Differences in activity of actinoporins are related with the hydrophobicity of their N-terminus . Ros U, Rodríguez-Vera W, Pedrera L, Valiente PA, Falcón SC, Lanio ME, García-Saez AJ, Alvarez C. Biochimie. 2015 Jun 29. pii: S0300-9084(15)00203-5. doi: 10.1016/j.biochi.2015.06.024. [Epub ahead of print] PMID:26134716

Minimalist model systems reveal similarities and differences between membrane interaction modes of MCL1 and BAK. Landeta O, Landajuela A, Garcia-Saez A, Basañez G. J Biol Chem. 2015 May 18. pii: jbc.M114.602193. [Epub ahead of print] PMID: 25987560

Formation of Disulfide Bridges Drives Oligomerization, Membrane Pore Formation and Translocation of Fibroblast Growth Factor 2 to Cell Surfaces. Müller HM, Steringer JP, Wegehingel S, Bleicken S, Münster M, Dimou E, Unger S, Weidmann G, Andreas H, García-Sáez AJ, Wild K, Sinning I, Nickel W. J Biol Chem. 2015 Feb 18. pii: jbc.M114.622456. [Epub ahead of print]

Toxicity of an α-pore-forming toxin depends on the assembly mechanism on the target membrane as revealed by single-molecule imaging. Subburaj Y, Ros U, Hermann E, Tong R, Garcia-Saez AJ. J Biol Chem. 2015 Feb 20;290(8):4856-65. doi: 10.1074/jbc.M114.600676. Epub 2014 Dec 18 PMID: 25525270

Structural model of active Bax at the membrane. Bleicken S, Jeschke G, Stegmueller C, Salvador-Gallego R, Garcia-Saez AJ, Bordignon E Mol Cell. 2014 Nov 20;56(4):496-505. doi:10.1016/j.molcel.2014.09.022. Epub 2014 Oct 30. PMID: 25458844

Dynamin-Related Protein 1 (Drp1) Promotes Structural Intermediates of Membrane Division. Ugarte-Uribe B., Mueller HM., Otsuki M., Nickel W., Garcia-Saez AJ. J Biol Chem. 2014 Sept 18 [Epub ahead of print]; doi: 10.1074/jbc.M114.575779

Automated analysis of Giant Unilamellar Vesicles using Circular Hough Transformation. Hermann E., Bleicken S., Subburaj Y., García-Sáez AJ. Bioinformatics. 2014 Feb 18.;

Proapoptotic Bax and Bak form stable protein-permeable pores of tunable size. Bleicken S, Landeta O, Landajuela A, Basañez G, García-Sáez AJ. J Biol Chem.2013 Nov 15; 288(46):33241-52. doi: 10.1074/jbc.M113.512087. Epub 2013 Oct 7. PMID: 24100034

Cardiolipin effects on membrane structure and dynamics. Unsay J, Cosentino K, Subburaj Y, García-Sáez AJ. Langmuir. 2013 Dec 23;29(51):15878-87. doi: 10.1021/la402669z. Epub 2013 Sep 10. PMID 23962277z

Caspase-8 binding to cardiolipin in giant unilamellar vesicles provides a functional docking platform for Bid. Jalmar O, François-Moutal L, García-Sáez AJ, Perry M, Granjon T, Gonzalvez F, Gottlieb E, Ayala-Sanmartin J, Klösgen B, Schwille P, Petit PX. PLoS One. 2013;8(2):e55250. doi: 10.1371/journal.pone.0055250. Epub 2013 Feb 13. PubMed PMID: 23418437; PubMed Central PMCID: PMC3572128.

Mechanistic Differences in the Membrane Activity of Bax and Bcl-xL Correlate with Their Opposing Roles in Apoptosis. Bleicken S, Wagner C, and García-Sáez AJ. Biophys J. 2013 Jan; 104(2):421-431

 

PI(4,5)P2 Dependent Oligomerization of Fibroblast Growth Factor 2 (FGF2) Triggers the Formation of a Lipidic Membrane Pore Implicated in Unconventional Secretion. Steringer JP, Bleicken S, Andreas H, Zacherl S, Laussmann M, Temmerman K, Contreras FX, Bharat TA, Lechner J, Muller HM, Briggs JA, García-Sáez AJ, Nickel W.

Dynamic interaction of cBid with detergents, liposomes and mitochondria. Bleicken S, García-Sáez AJ, Conte E, Bordignon E.

 

Oligomerization and pore formation by equinatoxin II inhibit endocytosis and lead to plasma membrane reorganization. García-Sáez AJ, Buschhorn SB, Keller H, Anderluh G, Simons K, Schwille P.

Destabilization exerted by peptides derived from the membrane-proximal external region of HIV-1 gp41 in lipid vesicles supporting fluid phase coexistence. Apellaniz B, García-Sáez AJ, Nir S, Nieva JL. BBA Biomembranes 2011 July 7; 1808(7):1797-1805.

Research Articles: Before 2011

 

Reviews, Book Chapters and Protocols