Interfaculty Institute of Biochemistry (IFIB)


Chemistry helps to bump off-target edits away

T. Stafforst*, Cell Chem. Biol. 2019, 26, 151-152.


Precise RNA editing by recruiting endogenous ADARs with antisense oligonucleotides

T. Merkle, S. Merz, P. Reautschnig, A. Blaha, Q. Li, P. Vogel, J. Wettengel, J. B. Li, T. Stafforst*, Nature Biotech. 2019, 37, 133-138.

press release en/dt

highlighted by Biopro BW

highlighted by Laborjournal

highlighted by C&EN


Critical review on engineering deaminases for site-directed RNA editing

P. Vogel, T. Stafforst*, Current Opin. Biotech. 2019, 55, 74-80.


Efficient and Precise Editing of Endogenous Transcripts with SNAP-tagged ADARs

P. Vogel, M. Moschref, Q. Li, T. Merkle, K. D. Selvasaravanan, J. B. Li, T. Stafforst*. Nature Methods 2018, 15, 535-38.

press release ENGLISH

press release GERMAN


Npom-protected NONOate enables light-triggered NO/cGMP signalling in primary vascular smooth muscle cells

A. S. Stroppel, M. Paolillo, T. Ziegler, R. Feil, T. Stafforst*. ChemBioChem 2018, 19, 1312-18.

*highlighted in ChemistryViews*

highlighted as "very important paper"


Switching protein localization by site-directed RNA editing under control of light

P. Vogel, A. Hanswillemenke, T. Stafforst*, ACS Synth. Biol. 2017, 6, 1642-9.

*open access*


Applying Human ADAR1p110 and ADAR1p150 for Site-Directed RNA Editing - G/C Substitution Stabilizes GuideRNAs Against Editing

M. Heep, P. Mach, P. Reautschnig, J. Wettengel, T. Stafforst*, Genes 2017, 8, 34 (special issue on RNA editing).

*open access*


Harnessing human ADAR2 for RNA repair – Recoding a PINK1 mutation rescues mitophagy

J. Wettengel, P. Reautschnig, S. Geisler, P. J. Kahle, T. Stafforst*, Nucl. Acids Res. 2017, 45, 2797-2808.

*open access*


The notorious R.N.A. in the Spotlight - Drug or Target for the Treatment of Disease

P. Reautschnig, P. Vogel, T. Stafforst*, RNA Biology 2017, 14, 651-668 (invited review).

*open access*


Site-directed RNA editing in vivo can be triggered by the light-driven assembly of an artificial riboprotein

A. Hanswillemenke, T. Kuzdere, P. Vogel, G. Jekely, T. Stafforst*, J. Am. Chem. Soc. 2015, 137, 15 875-81.

*open access*


Trendbericht Biochemie 2014: Biochemie natürlicher RNA-Modifikationen

T. Stafforst*, Nachrichten aus der Chemie 2015, 63, 306-9.


Upon the photostability of 8-nitro-cGMP and its caging as a 7-dimethylaminocoumarinyl ester

A. Samanta, M. Thunemann, R. Feil, T. Stafforst*, Chem. Commun. 2014, 50, 7120-23.


Site-directed RNA editing with Antagomir-deaminases - a tool to study protein and RNA function

P. Vogel, T. Stafforst*, ChemMedChem 2014, 9, 2021-25.


Improving Directed RNA Editing In Vitro and in Cell Culture by Chemical Modification of the guideRNA

P. Vogel, M. F. Schneider, J. Wettengel, T. Stafforst*, Angew. Chem. Int. Ed. 2014, 53, 6267-71.

German version:

Chemisch modifizierte guideRNAs verbessern die ortsgerichtete RNA-Editierung in vitro und in Zellkultur

Angew. Chem. 2014, 126, 6382-86.


Optimal guideRNAs for Re-directing Deaminase Activity of hADAR1 and hADAR2 in trans

M. F. Schneider, J. Wettengel, P. C. Hoffmann, T. Stafforst*, Nucl. Acids Res. 2014, 42, e87.

*open access*


Pyrene Chromophores for the Photoreversal of Psoralen Interstrand Crosslinks

J. M. Stadler, T. Stafforst*, Org. Biomol. Chem. 2014, 12, 5260-66.


Gerichtete Editierung – ein Werkzeug zur Reprogrammierung von RNA

T. Stafforst*, Biospektrum 2014, 20, 231-233.


The Selenocysteine Incorporation Machinery Allows the Dual Use of Sense Codons - A New Strategy to Expand the Genetic Code?

T. Stafforst*, ChemBioChem 2014, 15, 356-58.


Diskussionspapier: Biotechnologie - der Schlüssel zur Bioökonomie

Zukunftsforum der Dechema, Dechema 2014.


On the Sensitivity of Peptide Nucleic Acid Duplex Formation and Crystal Dissolution to a Variation of Force-Field Parameters

S. J. Bachmann, Z. Lin, T. Stafforst, W. F. van Gunsteren, J. Dolenc*, J. Chem. Theor. Comp. 2014, 10, 391-400.


Photoactivation of a Psoralen-blocked Luciferase Gene by Blue Light

T. Stafforst*, J. M. Stadler, Angew. Chem. Int. Ed. 2013, 52, 12448-51.

German version:

Photoaktivierung eines Psoralen-vernetzten Luciferasegens mit blauem Licht

Angew. Chem. 2013, 125, 12676-80.


An RNA-Deaminase Conjugate Selectively Repairs Point Mutations

T. Stafforst*, M. F. Schneider, Angew. Chem. Int. Ed. 2012, 51, 11166-9.

German version:

Ein RNA-Deaminase-Konjugat ermöglicht die selektive Reparatur von Punktmutationen

Angew. Chem. 2012, 124, 11329-32.


A FlAsH Reporter for Protein-Dimerization Triggers

T. Stafforst*, ChemBioChem 2012, DOI: 10.1002/cbic.201100787


Photolyase-like Repair of Psoralen-crosslinked Nucleic Acids

T. Stafforst*, D. Hilvert*, Angew. Chem. Int. Ed. 2011, 50, 9483-9486; Angew. Chem. 2011, 123, 9655-9658.

German version:

Photolyase-artige Reparatur Psoralen-quervernetzter Nucleinsäuren

Angew. Chem. 2011, 123, 9655-58.


Temperature-dependent intensity anomalies in amino acid esters: weak hydrogen bonds in protected glycine, alanine and valine

K. E. Otto, S. Hesse, T. N. Wassermann, C. A. Rice, M. A. Suhm*, T. Stafforst, U. Diederichsen, Phys. Chem. Chem. Phys. 2011, 13, 14119 - 14130.


Modulating PNA-DNA Hybridization by Light

T. Stafforst*, D. Hilvert*,

Angew. Chemie Int. Ed. 2010, 49, 9998-10001; Angew. Chem. 2010, 122, 10195-10198

German version:

Photokontrolle der PNA-DNA-Hybridisierung

Angew. Chem. 2010, 122, 10195-98.


Kinetic Characterization of Spiropyrans in Aqueous Media

T. Stafforst*, D. Hilvert, Chem. Commun. 2009, 287 - 288.


Synthesis of the chromophore of the Green Fluorescent Protein functionalized with alanine and N-(2-aminoethyl)glycine for incorporation into peptides and PNA

T. Stafforst, U. Diederichsen*, Eur. J. Org. Chem. 2007, 899 - 911.


Synthesis of acid-sensitive N-(2-aminoethyl)glycine-PNA oligomers via Fmoc/Bhoc strategy

T. Stafforst, U. Diederichsen*, Eur. J. Org. Chem. 2007, 681 - 688.


Thymine Oxetanes as Charge Traps for Chemical Monitoring of Nucleic Acid Mediated Transfer of Excess Electrons

T. Stafforst, U. Diederichsen*, Angew. Chem. Int. Ed. 2006, 45, 5376 - 5380.

German version:

Thyminoxetane als Ladungsfallen zum chemischen Nachweis des Nucleinsäure-vermittelten Überschusselektronentransfers

Angew. Chem. 2006, 118, 5502 – 06.


(6-4)-Photolyase activity requires a charge shift reaction

T. Stafforst, U. Diederichsen*, Chem. Commun. 2005, 3430 - 3432.


Aggregation behaviour of p-n-alkylbenzamidinium chloride surfactants

R. Talhout, T. Stafforst, J. B. F. N. Engberts*, J. Colloid Interface Sci. 2004, 276, 212 - 220