Pharmaceutical Biology

Original Research Publications

   
2017  
49. Basitta P, Westrich L, Rösch M, Kulik A, Gust B, Apel AK. (2017) AGOS: A Plug-and-Play Method for the Assembly of Artificial Gene Operons into Functional Biosynthetic Gene Clusters. ACS Synth Biol. 6(5):817-825.
2016  
48. Bauer JS, Hauck N, Christof L, Mehnaz S, Gust B, Gross H. (2016) The Systematic Investigation of the Quorum Sensing System of the Biocontrol Strain Pseudomonas chlororaphis subsp. aurantiaca PB-St2 Unveils aurI to Be a Biosynthetic Origin for 3-Oxo-Homoserine Lactones. PLoS One. 11(11):e0167002.
47. Bekiesch P, Franz-Wachtel M, Kulik A, Brocker M, Forchhammer K, Gust B, Apel AK. (2016) DNA affinity capturing identifies new regulators of the heterologously expressed novobiocin gene cluster in Streptomyces coelicolor M512. Appl Microbiol Biotechnol. 100(10):4495-509.
2015  
46. Medema MH…Gust B…Glöckner FO. (2015) Minimum Information about a Biosynthetic Gene cluster. Nature Chem Biol. 11(9):625-31.

2014

 
45. Rodolis MT, Mihalyi A, Ducho C, Eitel K, Gust B, Goss RJ, Bugg TD. (2014) Mechanism of action of the uridyl peptide antibiotics: an unexpected link to a protein-protein interaction site in translocase MraY. Chem Commun (Camb). 50(86):13023-5.

2013

 

44.

Tang X, Gross M, Xie Y, Kulik A, Gust B. (2013) Identification of Mureidomycin Analogues and Functional Analysis of an N-Acetyltransferase in Napsamycin Biosynthesis. Chembiochem. 14(17):2248-55.

43.

Tang X, Eitel K, Kaysser L, Kulik A, Grond S, Gust B. (2013) A two-step sulfation in antibiotic biosynthesis requires a type III polyketide synthase. Nature Chem Biol. 9(10):610-5.

42.

Jones AC, Gust B, Kulik A, Heide L, Buttner MJ, Bibb MJ. (2013) Phage p1-derived artificial chromosomes facilitate heterologous expression of the FK506 gene cluster. PLoS One. 8(7):e69319.

41.

Novotna J, Gust B, Kulik A, Spizek J, Heide L. (2013) Five gene products are required for assembly of the central pyrrole moiety of coumermycin A(1). J Ind Microbiol Biotechnol. 40(8):915-25.

40.

Holmes NA, Walshaw J, Leggett RM, Thibessard A, Dalton KA, Gillespie MD, Hemmings AM, Gust B, Kelemen GH. (2013) Coiled-coil protein Scy is a key component of a multiprotein assembly controlling polarized growth in Streptomyces. Proc Natl Acad Sci USA. 110(5):E397-406.

2012

 

39.

Jankowitsch F, Schwarz J, Rückert C, Gust B, Szczepanowski R, Blom J, Pelzer, S, Kalinowski J, Mack M. (2012) Genome sequence of the bacterium Streptomyces davawensis JCM 4913 and heterologous production of the unique antibiotic roseoflavin. J Bacteriol. 194(24):6818-27.

38.

Saleh O, Flinspach K, Westrich L, Kulik A, Gust B, Fiedler HP, Heide L. (2012) Mutational analysis of a phenazine biosynthetic gene cluster in Streptomyces anulatus 9663. Beilstein J Org Chem. 8:501-13.

2011

 

37.

Siebenberg S, Burkard N, Knuplesch A, Gust B, Grond S, Heide L. (2011) Two pathways for pyrrole formation in coumermycin A(1) biosynthesis: the central pyrrole moiety is formed from L-threonine. Chembiochem. 12(17):2677-85.

36.

Seeger K, Flinspach K, Haug-Schifferdecker E, Kulik A, Gust B, Fiedler HP, Heide L. (2011) The biosynthetic genes for prenylated phenazines are located at two different chromosomal loci of Streptomyces cinnamonensis DSM 1042. Microb Biotechnol. 4(2):252-62.

35.

Fowler-Goldsworthy K, Gust B, Mouz S, Chandra G, Findlay KC, Chater KF. (2011) The actinobacteria-specific gene wblA controls major developmental transitions in Streptomyces coelicolor A3(2). Microbiology. 157(Pt5):1312-28.

34.

Kaysser L, Tang X, Wemakor E, Sedding K, Hennig S, Siebenberg S, Gust B. (2011) Identification of a napsamycin biosynthesis gene cluster by genome mining. Chembiochem. 12(3):477-87.

33.

Siebenberg S, Kaysser L, Wemakor E, Heide L, Gust B, Kammerer B. (2011) Identification and structural elucidation of new caprazamycins from Streptomyces sp. MK730-62F2 by liquid chromatography/electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS). Rapid Commun Mass Spectrom. 25(4):495-502.

2010

 

32.

Flinspach K, Westrich L, Kaysser L, Siebenberg S, Gomez-Escribano JP, Bibb M, Gust B, Heide L. (2010) Heterologous expression of the biosynthetic gene clusters of coumermycin A1, clorobiocin and caprazamycins in genetically modified Streptomyces coelicolor strains. Biopolymers. 93(9):823-32.

31.

Kaysser L, Wemakor E, Siebenberg S, Salas JA, Sohng JK, Kammerer B, Gust B. (2010) Formation and attachment of the deoxysugar moiety and assembly of the gene cluster for caprazamycin biosynthesis. Appl Environ Microbiol. 76(12):4008-18.

30.

Zhao XQ, Gust B, Heide L. (2010) S-Adenosylmethionine (SAM) and antibiotic biosynthesis: effect of external addition of SAM and of overexpression of SAM biosynthesis genes on novobiocin production in Streptomyces. Arch Microbiol. 192(4):289-97.

29.

Siebenberg S, Bapat PM, Lantz AE, Gust B, Heide L. (2010) Reducing the variability of antibiotic production in Streptomyces by cultivation in 24-square deepwell plates. J Biosci Bioeng. 109(3):230-4.

28.

Kaysser L, Eitel K, Tanino T, Siebenberg S, Matsuda A, Ichikawa S, Gust B. (2010) A new arylsulfate sulfotransferase involved in liponucleoside antibiotic biosynthesis in streptomycetes. J Biol Chem. 285(17):12684-94.

27.

Dangel V, Westrich L, Smith MC, Heide L, Gust B. (2010) Use of an inducible promoter for antibiotic production in a heterologous host. Appl Microbiol Biotechnol. 87(1):261-9.

26.

Kaysser L, Siebenberg S, Kammerer B, Gust B. (2010) Analysis of the liposidomycin gene cluster leads to the identification of new caprazamycin derivatives. Chembiochem. 11(2):191-6.

2009

 

25.

Dangel V, Härle J, Goerke C, Wolz C, Gust B, Pernodet JL, Heide L. (2009) Transcriptional regulation of the novobiocin biosynthetic gene cluster. Microbiology. 155(Pt 12):4025-35.

24.

Kaysser L, Lutsch L, Siebenberg S, Wemakor E, Kammerer B, Gust B. (2009) Identification and manipulation of the caprazamycin gene cluster lead to new simplified liponucleoside antibiotics and give insights into the biosynthetic pathway. J Biol Chem. 284(22):14987-96.

23.

Saleh O, Gust B, Boll B, Fiedler HP, Heide L. (2009) Aromatic prenylation in phenazine biosynthesis: dihydrophenazine-1-carboxylate dimethylallyltransferase from Streptomyces anulatus. J Biol Chem. 284(21):14439-47.

2008

 

22.

Heide L, Westrich L, Anderle C, Gust B, Kammerer B, Piel J. (2008) Use of a halogenase of hormaomycin biosynthesis for formation of new clorobiocin analogues with 5-chloropyrrole moieties. Chembiochem. 9(12):1992-9.

21.

Dangel V, Eustáquio AS, Gust B, Heide L. (2008) novE and novG act as positive regulators of novobiocin biosynthesis. Arch Microbiol. 190(5):509-19.

20.

Wolpert M, Heide L, Kammerer B, Gust B. (2008) Assembly and heterologous expression of the coumermycin A1 gene cluster and production of new derivatives by genetic engineering. Chembiochem. 9(4):603-12.

2007

 

19.

Anderle C, Li SM, Kammerer B, Gust B, Heide L. (2007) New aminocoumarin antibiotics derived from 4-hydroxycinnamic acid are formed after heterologous expression of a modified clorobiocin biosynthetic gene cluster. J Antibiot (Tokyo). 60(8):504-10.

18.

Anderle C, Hennig S, Kammerer B, Li SM, Wessjohann L, Gust B, Heide L. (2007) Improved mutasynthetic approaches for the production of modified aminocoumarin antibiotics. Chem Biol. 14(8):955-67.

17.

Haagen Y, Unsöld I, Westrich L, Gust B, Richard SB, Noel JP, Heide L. (2007) A soluble, magnesium-independent prenyltransferase catalyzes reverse and regular C-prenylations and O-prenylations of aromatic substrates. FEBS Lett. 581(16):2889-93.

16.

Wolpert M, Gust B, Kammerer B, Heide L. (2007) Effects of deletions of mbtH-like genes on clorobiocin biosynthesis in Streptomyces coelicolor. Microbiology. 153(Pt 5):1413-23.

15.

Anderle C, Alt S, Gulder T, Bringmann G, Kammerer B, Gust B, Heide L. (2007) Biosynthesis of clorobiocin: investigation of the transfer and methylation of the pyrrolyl-2-carboxyl moiety. Arch Microbiol 187(3):227-37.

2006

 

14.

Haagen Y, Glück K, Fay K, Kammerer B, Gust B, Heide L. (2006) A gene cluster for prenylated naphthoquinone and prenylated phenazine biosynthesis in Streptomyces cinnamonensis DSM 1042. Chembiochem. 7(12):2016-27.

13.

Bralley P, Gust B, Chang S, Chater KF, Jones GH. (2006) RNA 3'-tail synthesis in Streptomyces: in vitro and in vivo activities of RNase PH, the SCO3896 gene product and polynucleotide phosphorylase. Microbiology. 152(Pt3):627-36.

12.

Khodakaramian G, Lissenden S, Gust B, Moir L, Hoskisson PA, Chater KF, Smith MC. (2006) Expression of Cre recombinase during transient phage infection permits efficient marker removal in Streptomyces. Nucleic Acids Res. 9;34(3):e20.

2005

 

11.

Ruban-Ośmiałowska, B., Gust, B., Chater, K.F., Jakimowicz, D. and Zakrzewska-Czerwińska, J. (2005) Strategia wykorzystująca PCR i homologiczną rekombinację do wprowadzania mutacji chromosomalnych u bakterii rodzaju Streptomyces, Postepy Mikrobiologii, 44(1):29-37.

10.

Eustáquio AS, Gust B, Galm U, Li SM, Chater KF, Heide L. (2005) Heterologous expression of novobiocin and clorobiocin biosynthetic gene clusters. Appl Environ Microbiol. 71(5):2452-9.

9.

Jakimowicz D, Gust B, Zakrzewska-Czerwinska J, Chater KF. (2005) Developmental-stage-specific assembly of ParB complexes in Streptomyces coelicolor hyphae. J Bacteriol. 187(10):3572-80.

2004

 

8.

Eustáquio AS, Gust B, Li SM, Pelzer S, Wohlleben W, Chater KF, Heide L. (2004) Production of 8'-halogenated and 8'-unsubstituted novobiocin derivatives in genetically engineered Streptomyces coelicolor strains. Chem Biol. 11(11):1561-72.

7.

Borovok I, Gorovitz B, Yanku M, Schreiber R, Gust B, Chater KF, Aharonowitz Y, Cohen G. (2004) Alternative oxygen-dependent and oxygen-independent ribonucleotide reductases in Streptomyces: cross-regulation and physiological role in response to oxygen limitation. Mol Microbiol. 54(4):1022-35.

2003

6.

Gust B, Challis GL, Fowler K, Kieser T, Chater KF. (2003) PCR-targeted Streptomyces gene replacement identifies a protein domain needed for biosynthesis of the sesquiterpene soil odour geosmin, Proc. Natl. Acad. Sci. USA 100:1541-6.

5.

Eustáquio AS, Luft T, Wang ZX, Gust B, Chater KF, Li SM, Heide L. (2003) Novobiocin biosynthesis: Inactivation of the putative regulatory gene novE and heterologous expression of genes involved in aminocoumarin ring formation, Arch. Microbiol. 180:25-32.

4.

Eustáquio AS, Gust B, Luft T, Li SM, Chater KF, Heide L. (2003) Chlorobiocin Biosynthesis in Streptomyces. Identification of the halogenase and generation of structural analogues, Chem Biol. 10:279-88.

2002

 

3.

Hesketh A, Fink D, Gust B, Rexer HU, Scheel B, Chater KF, Wohlleben W, Engels A. (2002) The GlnD and GlnK homologues of Streptomyces coelicolor A3(2) are functionally dissimilar to their nitrogen regulatory system counterparts from enteric bacteria, Mol Microbiol 46:319-30.

2001

2.

Gust B, Spatz K, Spychaj A, Redenbach M. (2001) Region-specific transcriptional activity in the genome of Streptomyces coelicolor A3(2), Appl Environ Microbiol 67:3598-602.

1999

 

1.

Redenbach M, Bibb M, Gust B, Seitz B, Spychaj A. (1999) The linear plasmid SCP1 of Streptomyces coelicolor A3(2) possesses a centrally located replication origin and shows significant homology to the transposon Tn4811, Plasmid 42:174-85.