21.10.2020

Genome-scale model of Corynebacterium glutamicum

In her master thesis, Martina Feierabend created an extended model of one of the most relevant industrial working microbes, C. glutamicum.

The bacterium Corynebacterium glutamicum can produce ʟ-glutamic acid under certain growth conditions. ʟ-Glutamic acid is used as a flavor enhancer, food supplement, or primary chemical raw material. It, therefore, plays an essential economic role with an annual production of over 2½ million tons. Due to metabolic development, the product range of C. glutamicum has been expanded to include all biogenic amino acids, vitamins, and more. Metabolic models of C. glutamicum already exist. These are to be supplemented with new metabolic data and expanded with data from new systems biology programs.

For this purpose, Martina Feierabend created general bacterial metabolism model, which was gradually supplemented by metabolites and reactions typical for this organism in her master thesis in collaboration with experts from the Research Center Jülich. The SBML document was supplemented and extended by MIRIAM standards (MIRIAM: Minimum Information Required in the Annotation of Models) and continuously improved by memote. The computer model was examined on different growth media and tested on ʟ-glutamic acid production. The simulation results show that C. glutamicum already has bacterial properties, but these are typical for the species.

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