Biochemistry
- phage display
- production and purification of recombinant proteins (Äkta-Express)
- affinity determination (BLItz, Biacore)
- Western blotting
- ELISA and bead-based ELISA (luminex)
- FACS scanning and sorting
- immunoprecipitation and Co-immunoprecipitation
Molecular biology
- cloning (classical, Gibson, mutagenesis)
- qRT-PCR
- lenti virus generation and production (S2)
- CRISPR technology
Cell biology
- cell line cultivation
- generation and characterization of stable chromobody cell lines
- lentiviral DNA transduction
- intracellular binding studies using fluorescent-2-hybrid assay
- 3D cell culture
- tumor cell migration and invasion studies
- confocal and video microscope suitable for FRAP and FRET analyses (Zeiss LSM510 and cell observer)
- fully automated high-throughput microscopy (ImageXpress, Molecular devices)
- high-content analysis
- compound and siRNA screening
