Peer-reviewed Publications

  1. Advancements in capacitance‐to‐digital converter‐based C4D technology for detection in capillary electrophoresis using amplified excitation voltages and comparison to classical and open‐source C4Ds
    H. G. Graf, B. M. Rudisch, J. Manegold, C. Huhn, Electrophoresis 2021,  doi: 10.1002/elps.202000394
  2. Behavioral and Developmental Changes in Brown Trout After Exposure to the Antidepressant Venlafaxine
    M. Ziegler, M. Banet, R. Bauer, H.-R. Köhler, S. Stepinski, S. Tisler, C. Huhn, C. Zwiener, R. Triebskorn, Frontiers Environm. Science 2021, 8, 586584, doi: 10.3389/fenvs.2020.586584
  3. Capillary electrophoresis-mass spectrometry for the direct analysis of glyphosate: method development and application to beer beverages and environmental studies.
    B. Wimmer, M. Pattky, L. Gulu Zada, M. Meixner, S.B. Haderlein, H.-P. Zimmermann, C. Huhn*, Anal. Bioanal. Chem. 2020, 412, 4967–4983, doi: 10.1007/s00216-020-02751-0
  4. Trends in sample preparation and separation methods for the analysis of very polar and ionic compounds in environmental water and biota samples
    S. Knoll, T. Rösch, C. Huhn*, Anal. Bioanal. Chem. 2020, 412, 6149-6165 doi: 10.1007/s00216-020-02811-5
  5. Challenges and applications of isotachophoresis coupled to mass spectrometry: A review
    Tanja Melzer, B. Wimmer, S. Bock, T.N. Posch, C. Huhn*, Electrophoresis 2020, 41, 1045-1059, doi: 10.1002/elps.201900454
  6. Development of a capillary electrophoresis–mass spectrometry method for the analysis of metformin and its transformation product guanylurea in biota
    S. Knoll, S. Jacob, S. Mieck, R. Triebskorn, T. Braunbeck, C. Huhn*, Anal. Bioanal. Chem. 2020, 412, 4985-4996, doi: 10.1007/s00216-020-02759-6
  7. Do environmentally relevant concentrations of fluoxetine and citalopram impair stress-related behavior in zebrafish (Danio rerio) embryos?
    F. Zindler, S. Stoll, L Baumann, S. Knoll, C. Huhn, T. Braunbeck, Chemosphere 2020, 261, 127753, doi: 10.1016/j.chemosphere.2020.127753
  8. Impact of the antidepressant citalopram on the behaviour of two different life stages of brown trout
    M. Ziegler, S. Knoll, H.-R. Köhler, S. Tisler, C. Huhn, C. Zwiener, R. Triebskorn, PeerJ 2020, 12;8:e8765, doi: 10.7717/peerj.8765
  9. Novel approach for the synthesis of a neutral and covalently bound capillary coating for capillary electrophoresis-mass spectrometry made from highly polar and pH-persistent N-acryloylamido ethoxyethanol
    M. Meixner, C. Huhn*, Anal. Bioanal. Chem. 2020, 412, 561-575; doi: 10.1007/s00216-019-02286-z
  10. Indirect CE‐UV detection for the characterization of organic and inorganic ions of a broad mobility and pK a range in engine coolants
    T. Rösch, J. Troffer, C. Huhn*, Electrophoresis 2019, 40: 2806-2809; doi: 10.1002/elps.201900198
  11. Ion mobility spectrometry as a fast screening tool for synthetic cannabinoids to uncover drug trafficking in jail via herbal mixtures, paper, food, and cosmetics
    S. Metternich, S. Zörntlein, T. Schönberger, C. Huhn*, Drug Testing and Analysis 2019, 11, 833-846; doi: 10.1002/dta.2565
  12. Effects of guanylurea, the transformation product of the antidiabetic drug metformin, on the health of brown trout (Salmo trutta f. fario)
    S. Jacob, S. Knoll, C. Huhn, H.-R. Köhler, S. Tisler, C. Zwiener, R. Triebskorn, Peer J. 2019, Jul 11;7:e7289; doi: 10.7717/peerj.7289
  13. More and enhanced glyphosate analysis is needed.
    C. Huhn*, Anal. Bioanal. Chem. 2018, 410, 3041-3045; doi: 10.1007/s00216-018-1000-3
  14. Does the antidiabetic drug metformin affect embryo development and the health of brown trout (Salmo trutta f. fario)?
    S. Jacob, A. Doetsch, S. Knoll, H.R. Köhler, E. Rogall, D. Stoll, S. Tisler, C. Huhn, T. Schwartz, C. Zwiener, R. Triebskorn, Environmental Sciences Europe 2018, 30, 48; doi: 10.1186/s12302-018-0179-4
  15. Glyphosate analysis using sensors and electromigration separation techniques as alternatives to gas or liquid chromatography
    G. Gauglitz, B. Wimmer, T. Melzer, C. Huhn*, Anal. Bioanal. Chem. 2018, 410, 725-746;  doi: 10.1007/s00216-017-0679-x
  16. Nano-sized zeolites as modulators of thiacloprid toxicity on Chironomus riparius
    C. S. Lorenz, A. J. Wicht, L. Guluzada, B. Crone, U. Karst, H. J. Lee, R. Triebskorn, S. B. Haderlein, C. Huhn, H. R. Köhler, Peer J. 2017, 18, 5:e3525; doi: 10.7717/peerj.3525
  17. Nano-sized Al2O3 reduces acute toxic effects of thiacloprid on the non-biting midge Chironomus riparius
    C. S. Lorenz, A.-J. Wicht, L. Guluzada, L. Luo, L. Jäger, B. Crone, Uwe Karst, R. Triebskorn, Y. Liang, R. Anwander, S. B. Haderlein, C. Huhn, H.-R. Köhler, PLoS ONE 2017, 12, e0176356; doi: 10.1371/journal.pone.0176356
  18. On-chip intermediate potential measurements for the control of electromigration in multi-channel networks in case of time-dependent potential changes
    D. Sydes, P.A. Kler, P. Zipfl, D. Lutz, H. Bouwes, C. Huhn*, Sens. Actuat. B: Chem. 2017, 240, 330-337; doi: 10.1016/j.snb.2016.08.152
  19. On-chip intermediate LED-IF-based detection for the control of electromigration in multichannel networks
    D. Sydes, P. A. Kler, H. Meyer, P. Zipfl, D. Lutz, C. Huhn*, Anal. Bioanal. Chem. 2016, 408, 8713-8725; doi: 10.1007/s00216-016-0033-8
  20. Zero-dead-volume interfaces for two-dimensional electrophoretic separations
    D. Sydes, P. A. Kler, M. Hermans, C. Huhn*, Electrophoresis 2016, 37, 3020-3024; doi: 10.1002/elps.201600262
  21. Spermidine, but not spermine, is essential for pigment pattern formation in zebrafish
    H. G. Frohnhöfer, S. Geiger-Rudolph, M. Pattky, M. Meixner, C. Huhn, H. M. Maischein, R. Geisler, I. Gehring, F. Maderspacher, C. Nüsslein-Volhard, U. Irion, Biol. Open. 2016, 5, 736-744; doi: 10.1242/bio.018721
  22. Statically adsorbed coatings for high separation efficiency and resolution in CE-MS peptide analysis: Strategies and implementation
    M. Pattky, K. Barkovits, O. Weiergräber, C. Huhn*, Meth. Mol. Biol. 2016, 1483, 53-75; doi: 10.1007/978-1-4939-6403-1_4
  23. Interlaboratory study to evaluate the robustness of capillary electrophoresis-mass spectrometry for peptide mapping
    C. Wenz, C. Barbas, Á López-Gonzálvez, A. Garcia, F. Benavente, V. Sanz-Nebot, T. Blanc, G. Freckleton, P. Britz-McKibbin, M. Shanmuganathan, F. de l'Escaille, J. Far, R. Haselberg, S. Huang, C. Huhn, M. Pattky, D. Michels, S. Mou, F. Yang, C. Neusuess, N. Tromsdorf, E. E. Baidoo, J. D. Keasling, S. S. Park, J. Sep. Sci. 2015, 38, 3262-3270; doi: 10.1002/jssc.201500551
  24. Structure characterization of unexpected covalent O-sulfonation and ion-pairing on an extremely hydrophilic peptide with CE-MS and FT-ICR-MS
    M. Pattky, S. Nicolardi, B. Santiago-Schübel, D. Sydes, Y. E. van der Burgt, A. N. Klein, J. Mohrlüder, K. Hänel, J. Kutzsche, S. A. Funke, D. Willbold, S. Willbold, C. Huhn*, Anal. Bioanal. Chem. 2015, 407, 6637-6655; doi: 10.1007/s00216-015-8826-8
  25. Electromigrative separation techniques in forensic science: combining selectivity, sensitivity, and robustness
    T. N. Posch, M. Pütz, N. Martin, C. Huhn*, Anal. Bioanal. Chem. 2015, 407, 23-58; doi: 10.1007/s00216-014-8271-0
  26. Column–coupling strategies for multidimensional electrophoretic separation techniques
    P. A. Kler, D. Sydes, C. Huhn*, Anal. Bioanal. Chem. 2014, 407, 119-138; doi: 10.1007/s00216-014-8099-7
  27. Non-aqueous electrolytes for isotachophoresis of weak bases and its application to the comprehensive preconcentration of the 20 proteinogenic amino acids in column-coupling ITP/CE-MS
    P. A. Kler, C. Huhn*, Anal. Bioanal. Chem. 2014, 406, 7163-7174; doi: 10.1007/s00216-014-8152-6
  28. Applicability of UV laser-induced solid-state fluorescence spectroscopy for characterization of solid dosage forms
    E. Woltmann, H. Meyer, D. Weigel, H. Pritzke, T. N. Posch, P. A. Kler, K. Schürmann, J. Roscher, C. Huhn*, Anal. Bioanal. Chem. 2014, 25, 6347-6362; doi: 10.1007/s00216-014-8074-3
  29. Column coupling isotachophoresis/capillary electrophoresis with mass spectrometric detection: characterization and optimization of microfluidic interfaces
    P. A. Kler, T. N. Posch, M. Pattky, R. M. Tiggelaar, C. Huhn*, J. Chromatogr. A 2013, 1297, 204-212; doi: 10.1016/j.chroma.2013.04.046
  30. Synthesis of nucleotide-activated disaccharides with recombinant β3-galactosidase C from Bacillus circulans
    C. Kamerke, M. Pattky, C. Huhn, L. Elling, J. Mol. Catal. B: Enzymatic 2013, 89, 73-81; 10.1016/j.molcatb.2012.12.014
  31. Desulfurized fuels from Athabasca bitumen and their polycyclic aromatic sulfur heterocycles. Analysis based on capillary electrophoresis coupled with TOF MS
    T. Nolte, T. N. Posch, C. Huhn, J. T. Andersson, Energy Fuels 2013, 27, 97-107; doi: 10.1021/ef301424d
  32. Advantages and limitations of a new cationic coating inducing a slow electroosmotic flow for CE-MS peptide analysis: a comparative study with commercial coatings
    M. Pattky, C. Huhn*, Anal. Bioanal. Chem. 2013, 405, 225-237; doi: 10.1007/s00216-012-6459-8
  33. Processes involved in sweeping under inhomogeneous electric field conditions as sample enrichment procedure in micellar electrokinetic chromatography
    M. El-Awady, C. Huhn, U. Pyell, J. Chromatogr. A 2012, 1264, 124-136; doi: 10.1016/j.chroma.2012.09.044
  34. Synthesis of UDP-activated oligosaccharides with commercial β-galactosidase from Bacillus circulans under microwave irradiation
    C. Kamerke, M. Pattky, C. Huhn, L. Elling; J. Mol. Catal. B: Enzymatic 2012, 79, 27-34; doi: 10.1016/j.molcatb.2012.04.003
  35. Non-aqueous capillary electrophoresis – mass spectrometry: A versatile, straightforward tool for the analysis of alkaloids from psychoactive plant extracts
    T. N. Posch, N. Martin, M. Pütz, C. Huhn*, Electrophoresis 2012, 33, 1557-1566; doi: 10.1002/elps.201100682
  36. Forensic analysis of mesembrine alkaloids in sceletium tortuosum by nonaqueous capillary electrophoresis mass spectrometry
    J. Roscher, T. N. Posch, M. Pütz, C. Huhn*, Electrophoresis 2012, 33, 1567-1570; doi: 10.1002/elps.201100683
  37. Hexapeptide library as a universal tool for sample preparation in protein glycosylation analysis
    C. Huhn*, L. R. Ruhaak, M. Wuhrer, A. M. Deelder, J. Proteomics 2012, 75, 1515-1528; doi: 10.1016/j.jprot.2011.11.028
  38. Alignment of laser-induced fluorescence and mass spectrometric detection traces using electrophoretic mobility scaling in CE-LIF-MS of labeled N-glycans
    C. Huhn*, L. R. Ruhaak, J. Mannhardt, M. Wuhrer, C. Neusüß, A. M. Deelder, H. Meyer; Electrophoresis 2012, 33, 563–566; doi: 10.1002/elps.201100367
  39. Implementation of a design of experiments to study the influence of the background electrolyte on separation and detection in non-aqueous capillary electrophoresis – mass spectrometry
    T. N. Posch, A. Müller, W. Schulz, M. Pütz, C. Huhn*, Electrophoresis 2012, 33, 583-598; doi: 10.1002/elps.201100381
  40. Robust and high-throughput sample preparation for (semi-)quantitative analysis of N-glycosylation profiles from plasma samples
    L. R. Ruhaak, C. Huhn*, C. A. M. Koeleman, A. M. Deelder, M. Wuhrer, Methods Mol. Biol. – Quantitative Methods in Proteomics 2012 , 893, 371-385; doi: 10.1007/978-1-61779-885-6_23
  41. Optimized workflow for preparation of APTS-labeled N-glycans allowing high-throughput analysis of human plasma glycomes using 48-channel multiplexed CGE-LIF
    L. R. Ruhaak, R. Hennig, C. Huhn, M. Borowiak, R. J. Dolhain, A. M. Deelder, E. Rapp, M. Wuhrer, J. Proteome Res. 2010, 9, 6655-6664; doi: 10.1021/pr100802f
  42. Glycan labeling strategies and their use in identification and quantification
    L. R. Ruhaak, G. Zauner, C. Huhn, C. Bruggink, A. M. Deelder, M. Wuhrer, Anal. Bioanal. Chem. 2010, 397, 3457-3481; doi: 10.1007/s00216-010-3532-z
  43. Protein glycosylation analysis with capillary-based electromigrative separation techniques
    M. Pattky, C. Huhn*, Bioanal. Rev. 2010, 2, 115-155; doi: 10.1007/s12566-010-0018-6
  44. Relevance and use of capillary coatings in capillary electrophoresis-mass spectrometry
    C. Huhn*, R. Ramautar, M. Wuhrer, G. W. Somsen, Anal. Bioanal. Chem. 2010, 396, 297-314; doi: 10.1007/s00216-009-3193-y
  45. Diffusion as a major source of band-broadening in field-amplified sample stacking under negligible electroosmotic flow velocity conditions
    C. Huhn, U. Pyell, J. Chromatogr. A 2010, 1217, 4476-4486; doi: 10.1016/j.chroma.2010.04.008
  46. Calibration-free concentration determination of charged colloidal nanoparticles and determination of effective charges by capillary isotachophoresis
    U. Pyell, W. Bücking, C. Huhn, B. Herrmann, A. Merkoulov, J. Mannhardt, H. Jungclas, T. Nann, Anal. Bioanal. Chem. 2009, 395, 1681-1691; doi: 10.1007/s00216-009-2887-5
  47. IgG glycosylation analysis
    C. Huhn, M. Selman, R. Ruhaak, A. M. Deelder, M. Wuhrer, Proteomics 2009, 9, 882-913; doi: 10.1002/pmic.200800715
  48. Hydrophilic interaction chromatography-based high-throughput sample preparation method for N-glycan analysis from total human plasma glycoproteins
    L. R. Ruhaak, C. Huhn, W.-J. Waterreus, A. R. de Boer, C. Neusüß, C. H. Hokke, A. M. Deelder, M. Wuhrer, Anal. Chem. 2008, 80, 6119-6126; doi: 10.1021/ac800630x
  49. Separation of very hydrophobic analytes by micellar electrokinetic chromatography IV. Modelling of the effec­tive electrophoretic mobility from carbon number equivalents and octanol-water partition coefficients
    C. Huhn, U. Pyell, J. Chromatogr. A 2008, 1198-1199, 208-214; doi: 10.1016/j.chroma.2008.04.061
  50. Separation of very hydrophobic analytes by micellar electrokinetic chromatography III. Charac­terization and optimization of the separation electrolyte using carbon numbers
    C. Huhn, M. Pütz, U. Pyell, Electrophoresis 2008, 29, 783-795; doi: 10.1002/elps.200700628
  51. Separation of very hydrophobic analytes by micellar electrokinetic chromatography II. Carbon number equiva­lents as analyte descriptors – influence of the composition of the separation elec­trolyte
    C. Huhn, M. Pütz, U. Pyell, Electrophoresis 2008, 29, 567-575; doi: 10.1002/elps.200700365
  52. Separation of very hydrophobic analytes by micellar electrokinetic chromatography I. Optimi­zation of the composi­tion of the sample solution for the determination of the aromatic ingredients of sassafras and other es­sential oils of forensic interest
    C. Huhn, M. Pütz, I. Holthausen, U. Pyell, Electrophoresis 2008, 29, 526-537; doi: 10.1002/elps.200700156
  53. Capillary electrophoresis - laser induced fluorescence – electrospray ionization-mass spectrometry: A case study
    C. Huhn, C. Neusüß, M. Pelzing, U. Pyell, J. Mannhardt, M. Pütz, Electrophoresis 2005, 26, 1389–1397; doi: 10.1002/elps.200410163
  54. Determination of tryptamine derivatives in illicit synthetic drugs by capillary electrophoresis and UV-laser-induced fluorescence detection
    C. Huhn, M. Pütz, N. Martin, R. Dahlenburg, U. Pyell, Electrophoresis 2005, 26, 2391-2401; doi: 10.1002/elps.200410313

Other Publications

  1. Relevanz ionischer Mikroschadstoffe – Quantifizierung von Metformin und dessen Transformationsprodukt Guanylharnstoff in Biota
    S. Knoll, S. Jacob, R. Triebskorn, C. Huhn, Mitteilungsblatt Fachgruppe Umweltchemie und Ökotoxikologie der Gesellschaft Deutscher Chemiker 2019, 25/2, S. 51-54
  2. Sassafras oils as precursors for the production of synthetic drugs: Profiling via MEKC-UVD
    C. Huhn, M. Pütz, R. Dahlenburg, U. Pyell, Beiträge zum XIV. GTFCh-Symposium 2005, 198-207
  3. Origin of MDMA and amphetamine precursors: Carbon-14 analysis of safrole, PMK, BMK
    M. Pütz, B. Herrmann, N. Martin, R. Dahlenburg, C. Huhn, U. Pyell, Beiträge zum XIV. GTFCh-Sym­posium 2005, 236-247
  4. Bestimmung von beta-Carbolinalkaloiden und Tryptaminen in Ayahuasca und pflanzlichen Drogen mit MEKC-UV-LIF
    M. Pütz, C. Huhn, R. Dahlenburg, U. Pyell, Beiträge zum XIII. GTFCh-Sym­posium, 2003, 275-282
  5. Chirale Analytik mit Kapillarelektrophorese
    C. Huhn*, M. Bauerfeind, GIT Spezial – Separation 1/2010
  6. Das Süße im EPO - Glykoproteincharakterisierung mit Kapillarelektrophorese-Massenspektrometrie
    C. Neusüß, S. Vaas, C. Huhn, GIT Laborfachzeitschrift 2008, 1, 22-24

Lectures at national and international conferences (Lecturer)

  1. Analytische Chemie und Ökotoxikologie: Aufnahme, Transfer und Metabolismus von Mikroschadstoffen und der Einfluss von Nanopartikeln
    Anakon 2019, Münster, keynote, invited lecture
  2. Multidimensional separations in the electric field - Instrumental innovations
    15th International Symposium on Hyphenated Techniques in Chromatography 2018, Cardiff, Award winner lecture
  3. Nonaqueous capillary electrophoresis mass spectrometry for the analysis of the micropol-lutant metformin in biota and optimization of the geometry of the capillary tip 
    CE Forum 2018, Pfinztal
  4. Versatility of capillary electrophoresis – applications and instrumental innovations for multidimensional separations
    analytica conference 2016, München, invited lecture
  5. Herbizide in der Umwelt: Glyphosat vs. Atrazin
    Abwassersymposium Fraunhofer IGB 2016, invited lecture
  6. Kapillarelektrophorese als spannendes Tool für die Analyse komplexer Proben
    Anakon 2013, Essen, Preisträgervortrag
  7. Multidimensional separations using column coupling ITP-CE-MS
    Dutch CE User Meeting 2013, Breda, eingeladener Vortrag
  8. Hexapeptide library as a universal tool for sample preparation in glycan analysis of human plasma MSB2012, Genf
  9. Glycosylation analysis of proteins by CE-MS
    CEPharm 2011, Amelia Island, Florida, eingeladener Vortrag
  10. From small ions to proteins, from forensic to clinical analysis: About the versatility of CE and CE-MS
    CIA 2011, Berlin, Award winner lecture
  11. Adsorbed cationic capillary coatings for (glyco)peptide separation
    ITP 2011, Tiflis
  12. The need for novel capillary coatings – optimization of CE-MS methods for (glyco)peptide analysis CEPharm 2008, San Francisco, eingeladener Vortrag
  13. Online preconcentration techniques in capillary electrophoresis – Definition of the enrichment factor
    ISC 2008, Münster
  14. Neue Verfahren zur Charakterisierung von Nanopartikeln
    Analytica Forum 2008, Messe München
  15. Modellierung und Optimierung von MEKC Trennungen mittels homologer Reihen
    Anakon 2007, Jena
  16. Einsatz homologer Reihen in der MEKC
    Doktorandenseminar des Arbeitskreises Separation Sci­ence der GDCh 2007, Hohenroda
  17. Sassafras Oils as Precursors for the Production of Synthetic Drugs: Profiling via MEKC-UV/(UV)LIF
    XIV. GTFCh-Symposium 2005, Mosbach
  18. Analytik biogener Drogen am Beispiel von Ayahuasca mittels CZE und MEKC über UV-laser-indu­zierte Nativfluoreszenz Detektion
    Anakon 2003, Konstanz
  19. Analytik biogener Drogen am Beispiel von Ayahuasca mittels CZE und MEKC über UV-laser-indu­zierte Nativfluoreszenz Detektion
    Doktorandenseminar des Arbeitskreises Separation Science der GDCh 2003, Hohenroda, Award for the beste lecture

Invitations to lecture as part of the analytics seminar: Merck 2019, Universität Mainz 2016, ISAS 2015, Comenius Universität Bratislava 2015, Bosch 2015, Agilent Usermeeting 2014, IKP Stuttgart 2014, IGB Stuttgart 2014, Hochschule Niederrhein 2014, Universität Rostock 2014, BASF 2013, Proteom Center Bochum 2012, Universität Leipzig 2011, RWTH Aachen 2011, Universität Duisburg-Essen 2011, Universität Münster 2010 und 2011, Paul-Ehrlich-Institut 2010

Several lectures by PhD students and post-docs at national and international conferences


  1. Tutorial Trenn- und Kopplungstechniken, Wasseranalytikworkshop 2014, Tübingen
  2. Capillary electrophoresis - mass spectrometry as a versatile tool for the analysis of complex samples, Workshop during DGMS 2014, Frankfurt
  3. Workshop on CE-MS, CEPharm 2011, Amelia Island, Florida
  4. Characterization of glycoproteins, Workshop during der 3rd European Summer School in Proteomic Basics - “Quantification and Post-Translational Modifications of Proteins“ 2009 , Brixen
  5. Introduction to capillary electrophoresis, Doktorandenseminar des Arbeitskreises Separation Science der GDCh 2009, Hohenroda
  6. Fundamentals of capillary electrophoresis and related techniques and their application in forensic toxicology, ISC 2008, Münster


C. Huhn, H. Meyer, J. Mannhardt, C. Neusüß, Deutsches Pa­tent- und Markenamt 202008009113.6.
C. Huhn, P. Kler, D. Lutz, P. Zipfl, Patentanmeldung Vorrichtung und Verfahren zur kontaktlosen Potentialmessung bei elektromigrativen Trenntechniken 102014222421.7 in november 2014