Interfakultäres Institut für Mikrobiologie und Infektionsmedizin

Publications

2018

  1. Robertsen, H.L., E.M. Musiol-Kroll, L. Ding, K.J. Laiple, T. Hofeditz, W. Wohlleben, S.Y. Lee, S. Grond, and T. Weber. 2018. Filling the Gaps in the Kirromycin Biosynthesis: Deciphering the Role of Genes Involved in Ethylmalonyl-CoA Supply and Tailoring Reactions. Sci. Reports 8: 3230.

  2. Spohn, M., S. Edenhart, M. Alanjary, N. Ziemert, D. Wibberg, J. Kalinowski, T. Niedermeyer, E. Stegmann, and W. Wohlleben. 2018. Identification of a unique aminoploycarboxylic acid siderophore gene cluster and its product by an in silico approach. Metallomics, in press
  3. Schwarz, P.N., L.Roller, W. Wohlleben, A. Kulik and E. Stegmann. 2018. Engineering metabolic pathways in Amycolatopsis japonicum for the optimization of the precursor supply for heterologous brasilicardin congeners production. Synth. Syst. Biotechnol. 3:56-63.
  4. Schwarz, P.N., A. Buchmann, L. Roller, A. Kulik, H. Gross, W. Wohlleben, and E. Stegmann. 2018. The immunosuppressant brasilicardin: Determination of the biosynthetic gene cluster in the heterologous host Amycolatopsis japonicum. Biotechnol. J., 2018, 13, 1700527.

2017

  1. Wohlleben, W., A. Bera, Y. Mast, and E. Stegmann. 2017. Regulation of Secondary Metabolites of Actinobacteria in “Biology and Biotechnology of Actinobacteria" Eds. J. Wink, F. Mohammadipanah, J. Hamedi, Springer Int. Publishing, pp. 181- 232.
  2. Meng, J., R. Feng, G. Zheng, M. Ge, Y. Mast, W. Wohlleben, J. Gao, W. Jiang, and Y. Lu. 2017. Improvement of pristinamycin I (PI) production in Streptomyces pristinaespiralis by metabolic engineering approaches. Synth. Syst. Biotechnol. 2:130-136.
  3. Jahn, L.*, T. Schafhauser*, D. Wibberg*, C. Rückert, A. Winkler, A. Kulik, T. Weber, L. Flor, K.-H. van Pée, J. Kalinowski, J. Ludwig-Müller, and W. Wohlleben. 2017. Linking secondary metabolites to biosynthesis genes in the fungal endophyte Cyanodermella asteris: The anti-cancer bisanthraquinone skyrin. J. Biotechnol. 257:233-239.
  4. Schmidt, K., J. Schmidtke, Y. Mast, E. Waldvogel, W. Wohlleben, F. Klemke, W. Lockau, T. Hausmann, M. Hühns, and I. Broer. 2017. Comparative statistical component analysis of transgenic, cyanophycin producing potatoes in greenhouse and field trials. Transgenic Res. 26:529-539.
  5. Kittilä, T., C. Kittel, J. Tailhades, D. Butz, M. Schoppet, A. Büttner, R.J.A. Goode, R.B. Schittenhelm, K.-H. van Pée, R.D. Süßmuth, W. Wohlleben, M.J. Cryle, and E. Stegmann. 2017. Halogenation of glycopeptide antibiotic biosynthesis occurs at the amino acid level during non-ribosomal peptide synthesis. Chem. Sci. 8:5992-6004.
  6. Tsypik, O., R. Makitrynskyy, A. Bera, L. Song, W. Wohlleben, L. Fedorenko, and B. Ostash. 2017. Role of GntR Family Regulatory Gene SCO1678 in Gluconate Metabolism in Streptomyces coelicolor M145. BioMed Research International 2017, ID 9529501.
  7. Krysenko, S., N. Okoniewski, A. Kulik, A. Matthews, J. Grimpo, W. Wohlleben, and A. Bera. 2017. Gamma-glutamylpolyamine synthetase GlnA3 is involved in the first step of polyamine degradation pathway in Streptomyces coelicolor M145. Front. Microbiol. 8:726.
  8. Jahn, L., T. Schafhauser, S. Pan, T. Weber, W. Wohlleben, D.P. Fewer, K. Sivonen, L. Flor, K.-H. van Pée, T. Caradec, P. Jacques, M.M.E. Huijbers, W.J.H. van Berkel, and J. Ludwig-Müller. 2017. Cyanodermella asteris sp. nov. (Ostropales) from the inflorescence axis of Aster tataricus. . Mycotaxon 132: 107–123.
  9. Musiol-Kroll, E.M., F. Zubeil, T. Schafhauser, T. Härtner, A. Kulik, J. McArthur, I. Koryakina, W. Wohlleben, S. Grond, G. Williams, S.Y. Lee, and T. Weber. 2017. Polyketide bioderivatization using the promiscuous acyltransferase KirCII. ACS Synth. Biol. 6:421-427.

2016

  1. Buchmann, A., M. Eitel, P. Koch, P.N. Schwarz, E. Stegmann, W. Wohlleben, M. Wolanski, M. Krawiec, J. Zakrzewska-Czerwinska, C. Méndez, A. Botas, L.E. Núnez, F. Moris, J Cortés, and H. Gross. 2016. High-Quality Draft Genome Sequence of the Actinobacterium Nocardia terpenica IFM 0406, Producer of the Immunosuppressant Brasilicardins, Using Illumina and PacBio Technologies. Genome Announcement 4:e01391-16.
  2. Amin, R., M. Franz-Wachtel, Y. Tiffert, M. Heberer, M. Meky, Y. Ahmed, A. Matthews, S. Krysenko, M. Jacobi, M. Hinder, J. Moore, N. Okoniewski, B. Macek, W. Wohlleben, and A. Bera. 2016. Post-translational serine/threonine phosphorylation and lysine acetylation: a novel regulatory aspect of the global nitrogen response regulator GlnR in Streptomyces coelicolor M145. Front. Microbiol. 3:38. eCollection 2016.
  3. Wohlleben, W., Y. Mast, E. Stegmann, and N. Ziemert. 2016. Antibiotic Drug Discovery. Microbial. Biotechnol. 9:541-548.
  4. Sigle, S., N. Steblau, W. Wohlleben, and G. Muth. 2016. A toolbox to measure changes in the cell wall glycopolymer composition during differentiation of Streptomyces coelicolor A3(2). J. Microb. Meth. 128:52-57.
  5. Kilian, R., H.-J. Frasch, A. Kulik, W. Wohlleben, and E. Stegmann, 2016. The VanRS homologous two component system VlnRS of the glycopeptide producer Amycolatopsis balhimycina activates transcription of the vanHAX genes in Streptomyces coelicolor, but not in A. balhimycina. Microb. Drug Resist. 22:499-509.
  6. Sigle, S., N. Steblau, W. Wohlleben, and G. Muth. 2016. The polydiglycosylphosphate transferase PdtA (SCO2578) of Streptomyces coelicolor A3(2) is crucial for proper sporulation and apical tip extension under stress conditions. Appl. Environ. Microbiol. 82:5661-5672.
  7. Schafhauser T., N. Kirchner, A. Kulik, M.M.E. Huijbers, L. Flor, W.J.H. van Berkel, T. Caradec, D.P. Fewer, H. Gross, P. Jacques, L. Jan, J. Jokela, J. Ludwig-Müller, K. Sivonen, T. Weber, W. Wohlleben, and K.-H. van Pée. 2016. The cyclochlorotine mycotoxin is produced by the CctN nonribosomal peptide synthetase in Talaromyces islandicus („Penicillium islandicum“). Environ. Microbiol. 18:3728-3741.
  8. Spohn, M., W. Wohlleben, and E. Stegmann. 2016. The zincophore ethylenediamine-disuccinate (EDDS): A new way to identify biosynthetic genes. Environ. Microbiol. 18:1249-63.
  9. Pozzi, R., M. Cole, D. Linke, A. Kulik, M. Nega, W. Wohlleben, and E. Stegmann. 2016. Distinct mechanisms contribute to immunity in the lantibiotic NAI-107 producer strain Microbispora ATCC PTA-5024, Environ. Microbiol. 18:118-32.
  10. Iftime, D., A. Kulik, T. Härtner, S. Rohrer, E. Stegmann, T. Niedermeyer, T. Weber, and W. Wohlleben. 2016. Identification and activation of novel biosynthetic gene clusters by genome mining in the kirromycin producer Streptomyces collinus Tü365. J. Ind. Microbiol. Biotechnol. 43:277-291.

2015

  1. Iftime, D., A. Kulik, M. Jasyk, R. Süssmuth, W. Wohlleben, and T. Weber. 2015. Streptocollin, the first type IV lanthipeptide produced by the actinomycete Streptomyces collinus Tü 365. ChemBioChem 16:2615-23.
  2. Medema, M.H., R. Kottmann, P. Yilmaz, M. Cummings, J.B. Biggins, K. Blin, I. de Bruijn, Y.H. Chooi, J. Claesen, R.C. Coates, P. Cruz-Morales, S. Duddela, S. Düsterhus, D.J. EdwardsJ, D.P. Fewer, N. Garg, C. Geiger, J.P. Gomez-Escribano, A. Greule, M. Hadjithomas, A.S. Haines, E.J. HelfrichJ, M.L. Hillwig, K. Ishida, A.C. Jones, C.S. Jones, K. Jungmann, C. Kegler, H.U. Kim, P. Kötter, D. Krug, J. Masschelein, A.V. Melnik, S.M. Mantovani, E.A. Monroe, M. Moore, N. Moss, H.W. Nützmann, G. Pan, A. Pati, D. Petras, F.J. Reen, F. Rosconi, Z. Rui, Z. Tian, N.J. Tobias, Y. Tsunematsu, P. Wiemann, E. Wyckoff, X. Yan, G. Yim, F. Yu, Y. Xie, B. Aigle, K.A. Apel, C.J. Balibar, E.P. Balskus, F. Barona-Gómez, A. Bechthold, H.B. Bode, R. Borriss, S.F. Brady, A.A. Brakhage, P. Caffrey, Y.Q. Cheng, J. Clardy, R.J. Cox, R. De Mot, S. Donadio, M.S. Donia, W.A. van der Donk, P.C. Dorrestein, S. Doyle, A.J. Driessen, M. Ehling-Schulz, K.D. Entian, M.A. Fischbach, L. Gerwick, W.H. Gerwick, H. Gross, B. Gust, C. Hertweck, M. Höfte, S.E: Jensen, J. Ju, L. Katz, L. Kaysser, J.L. Klassen, N.P. Keller, J. Kormanec, O.P. Kuipers, T. Kuzuyama, N.C. Kyrpides, H.J. Kwon, S. Lautru, R. Lavigne, C.Y. Lee, B. Linquan, X. Liu, W. Liu, A. Luzhetskyy, T. Mahmud, Y. Mast, C. Méndez, M. Metsä-Ketelä, J. Micklefield, D.A. Mitchell, B.S. Moore, L.M. Moreira, R. Müller, B.A. Neilan, M. Nett, J. Nielsen, F. O'Gara, H. Oikawa, A. Osbourn, M.S. Osburne, B. Ostash, S.M. Payne, J.L. Pernodet, M. Petricek, J. Piel, O. Ploux, J.M. Raaijmakers, J.A. Salas, E.K. Schmitt, B. Scott, R.F. Seipke RF, B. Shen, D.H. Sherman, K. Sivonen, M.J. Smanski, M. Sosio, E. Stegmann, R.D. Süssmuth, K. Tahlan, C.M. Thomas, Y. Tang, A.W. Truman, M. Viaud, J.D. Walton, C.T. Walsh, T. Weber, G.P. van Wezel, B. Wilkinson, J.M. Willey, W. Wohlleben, G.D. Wright, N. Ziemert, C. Zhang, S.B. Zotchev, R. Breitling, E. Takano, F.O. Glöckner. 2015. Minimum Information about a Biosynthetic Gene Cluster. Nat. Chem. Biol. 11:625-631.
  3. Schafhauser, T., D. Wibberg, C. Rückert, A. Winkler, L. Flor, K.-H. van Pée, D. P. Fewer, K. Sivonen, L. Jahn, J. Ludwig-Müller, T. Caradec, P. Jacques, M.M.E. Huijbers, W.J.H. van Berkel, T. Weber, W. Wohlleben, and J. Kalinowski. 2015. Draft genome sequence of Talaromyces islandicus ("Penicillium islandicum") WF-38-12, a neglected mold with significant biotechnological potential. J. Biotechnol. 211:101-102.
  4. Ladwig, N., F. Hezel, M. Franz-Wachtel, B. Soufi, B. Macek, W. Wohlleben, and G. Muth. 2015. Control of morphological differentiation of Streptomyces coelicolor A3(2) by phosphorylation of MreC and PBP2. PLoS ONE 10:e0125425.
  5. Weber, T., K. Blin, S. Duddela, D. Krug, H.U. Kim, R. Bruccoleri, S.Y. Lee, M.A. Fischbach, R. Müller, W. Wohlleben, R. Breitling, E. Takano, and M.H. Medema. 2015. antiSMASH3.0 – A comprehensive resource for the genome mining of biosynthetic gene clusters. Nucleic Acids Res. 43(W1):W237-43.
  6. Braun, V., F. Götz, J.E. Schultz, and W. Wohlleben. 2015. Editorial: The bacterial cell envelope: Structure, function, and infection interface. Int. J. Med. Microbiol. 305:175-177.
  7. Sigle, S., N. Ladwig, W. Wohlleben, and G. Muth. 2015. Synthesis of the spore envelope in the developmental life cycle of Streptomyces coelicolor. Int. J. Med. Microbiol. 305:183-189.

2014

  1. Stegmann, E., M. Spohn, H. Gert, T. Weber, W. Wohlleben, J. Kalinowski, and C. Rückert. 2014. Complete Genome Sequence of the Actinobacterium Amycolatopsis japonica MG417-CF17T (=DSM 44213T) producing (S,S)-N,N'-ethylenediaminedisuccinic acid. J.Biotechnol. 189C:46-47.
  2. Spohn, M., N. Kirchner, A. Kulik, A. Jochim, F. Wolf, P. Muenzer, O. Borst, H. Gross, W. Wohlleben, and E. Stegmann. 2014. Overproduction of ristomycin A by activation of a silent gene cluster in Amycolatopsis japonicum MG417-CF17. Antimicrob. Agents Chemother. 58:6185-96.
  3. Blin, K., D. Kazempour, W. Wohlleben, and T. Weber. 2014. Improved Lanthipeptide Detection and Prediction for antiSMASH. PLoS One 9:e89420.
  4. Michta, E., W. Ding, S. Zhu, K. Blin, H. Ruan, R. Wang, W. Wohlleben, and Y. Mast. 2014. Proteomic approach to reveal the regulatory function of aconitase AcnA in oxidative stress response in the antibiotic producer Streptomyces viridochromogenes Tü494. PLoS ONE 9:e87905.
  5. Mast, Y., and W. Wohlleben. 2014. Streptogramins - Two are better than one! Int. J. Med. Microbiol. 304:44-50.

2013

  1. Rückert, C., R. Szczepanowski, A. Albersmeier, A. Goesmann, D. Iftime, E.-M. Musiol, K. Blin, W. Wohlleben, A. Pühler, J. Kalinowski, and T. Weber. 2013. Complete genome sequence of the kirromycin producer Streptomyces collinus Tü 365 consisting of a linear chromosome and two linear plasmids. J. Biotechnol. 168:739-40.
  2. Musiol, E.-M., A. Greule, T. Härtner, W. Wohlleben, and T. Weber. 2013. The AT2 Domain of KirCI Loads Malonyl Extender Units to the ACPs of the Kirromycin PKS. ChemBioChem 14:1343-52.
  3. Wang, R., H. Zhu, G. Zheng, Y. Mast, S. Yang, W. Wohlleben, Y. Lu, and W. Jiang. 2013. Identification of two-component system AfsQ1/Q2 regulon and its cross-regulation with GlnR in Streptomyces coelicolor. Mol. Microbiol. 87:30–48.
  4. Sola-Landa, A., A. Rodríguez-García, R. Amin, W. Wohlleben, and J.F. Martín. 2013. Competition between the GlnR and PhoP regulators for the glnA and amtB promoters in Streptomyces coelicolor. Nucleic Acids Res. 41:1767-82.

1979 - 2012

  1. Michta, E., K. Schad, K. Blin, R. Ort-Winklbauer, M. Röttig, O. Kohlbacher, W. Wohlleben, E. Schinko, and Y. Mast. 2012. The bifunctional role of aconitase in Streptomyces viridochromogenes Tü494. Environ. Microbiol. 14:3203-2319.

  2. Wohlleben, W., Y. Mast, G. Muth, M. Röttgen, E. Stegmann, and T. Weber. 2012. Synthetic Biology of Secondary Metabolite Biosynthesis in Actinomycetes: Engineering Precursor Supply as a Way to Optimize Antibiotic production. FEBS Lett. 586:2171-2176.

  3. Iqbal, M., Y. Mast, R. Amin, D. Hodgson, STREAM consortium, W. Wohlleben, and N. Burroughs. 2012. Extracting regulator activity profiles by integration of de novo motif search and expression data: characterising the key regulators in the metabolic switch of Streptomyces coelicolor A3(2). Nucleic Acids Res. 40:5227-5239.
  4. Amin, R., J. Reuther, A. Bera, W. Wohlleben, and Y. Mast. 2012. The novel GlnR-target gene nnaR is involved in nitrate/nitrite assimilation in Streptomyces coelicolor. Microbiology 158:1172-1182.
  5. Vongsangnak, W., L.F. Figueiredo, J. Förster, T. Weber, J. Thykaer, E. Stegmann, W. Wohlleben, and J. Nielsen. .2012. Genome-Scale Metabolic Representation of Amycolatopsis balhimycina. Biotechnol Bioeng. 109:1798-1807.
  6. Thomas, L., D.A. Hodgson, A. Wentzel, K. Nieselt, T.E. Ellingsen, J. Moore, E.R. Morrissey, R. Legaie, The STREAM Consortium, W. Wohlleben, A. Rodriguez-Garcia, J.F. Martín, N.J. Burroughs, E.M.H. Wellington, and M.C.M. Smith. 2012. Metabolic switches and adaptations deduced from the proteomes of Streptomyces coelicolor wild type and phoP mutant grown in batch culture. Mol. Cell. Proteomics. 11:M111.013797.
  7. Waldvogel, E., A. Herbig, F. Battke, R. Amin, M. Nentwich, K. Nieselt, T.E. Ellingsen, A. Wentzel, D.A. Hodgson, W. Wohlleben, and Y. Mast. 2011. The PII protein GlnK is a pleiotropic regulator for morphological differentiation and secondary metabolism in Streptomyces coelicolor. Appl. Microbiol. Biotechnol. 92:1219-1236.
  8. Kleinschnitz, E.-M., A. Latus, S. Sigle, I. Maldener, W. Wohlleben, and G. Muth. 2011. Genetic Analysis of SCO2997, Encoding a TagF Homologue, Indicates a Role for Wall Teichoic Acids in Sporulation of Streptomyces coelicolor A3(2). J. Bacteriol. 193: 6080-6085.
  9. Pavlidou, M., E. Pross, E.-M. Musiol, A. Kulik, W. Wohlleben, and T. Weber. 2011. The phosphopantetheinyl transferase KirP activates the ACP and PCP domains of the kirromycin NRPS/PKS of Streptomyces collinus Tü 365. FEMS Microbiol. Lett. 319:26-33.
  10. Vogelmann, J., M. Ammelburg, C. Finger, J. Guezguez, D. Linke, Y. Stierhof, W. Wohlleben, and G. Muth. 2011. Conjugal plasmid transfer by a chromosome segregation mechanism. EMBO J. 30:2246-2254.
  11. Schäberle, T., W. Vollmer, H.-J. Frasch, S. Hüttel, M. Röttgen, A.-K. von Thaler, W. Wohlleben, and E. Stegmann. 2011. Self-resistance and cell wall composition in the glycopeptide producer Amycolatopsis balhimycina. Antimicrob. Agents Chemother. 55:4283-4289.
  12. Musiol, E.-M., T. Härtner, A. Kulik, J. Moldenhauer, J. Piel, W. Wohlleben, and T. Weber. 2011. Supramolecular templating in kirromycin biosynthesis: the acyltransferase KirCII loads ethylmalonyl-CoA extender onto a specific ACP of the trans-AT PKS. Chem. Biol. 18:438-444.
  13. Tiffert, Y., M. Franz-Wachtel, J. Madlung, C. Fladerer, A. Nordheim, J. Reuther, W. Wohlleben, and Y. Mast. 2011. Proteomic analysis of the GlnR-mediated response to nitrogen limitation in Streptomyces coelicolor M145. Appl. Microbiol. Biotechnol. 89:1149-1159.
  14. Mast, Y., W. Wohlleben, and E. Schinko. 2011. Identification and Functional Characterization of Phenylglycine Biosynthetic Genes Involved in Pristinamycin Biosynthesis in Streptomyces pristinaespiralis. J. Biotechnol. 155: 63-67.
  15. Kleinschnitz, E.-M., A. Heichlinger, K. Schirner, J. Winkler, A. Latus, I. Maldener, W. Wohlleben, and G. Muth. 2011. Proteins encoded by the mre gene cluster in Streptomyces coelicolor A3(2) co-operate in spore wall synthesis. Mol. Microbiol. 79:1367-1379.
  16. Heichlinger, A., M. Ammelburg, E.-M. Kleinschnitz, A. Latus, I. Maldener, K. Flärdh, W. Wohlleben, and G. Muth. 2011. The MreB-like Mbl protein of Streptomyces coelicolor A3(2) depends on MreB for proper localization and contributes to spore wall synthesis. J. Bacteriol. 193:1533-1542.
  17. Wohlleben, W., Y. Mast, and J. Reuther. 2011. Regulation of Nitrogen Assimilation in Streptomycetes and other Actinobacteria. p. 125-136. In Streptomyces Molecular Biology and Biotechnology. P. Dyson, ed., Caister Academic Press, Norfolk, UK.
  18. Vogelmann, J., W. Wohlleben, and G. Muth. 2011. Streptomyces conjugative genetic elements. P. 27-42. In Streptomyces Molecular Biology and Biotechnology. P. Dyson, ed., Caister Academic Press, Norfolk, UK.
  19. Battke, F., A. Herbig, A. Wentzel, O. Jakobsen, M. Bonin, D. Hodgson, W. Wohlleben, T. Ellingsen, SysMO Stream Consortium, and K. Nieselt. 2011. A Technical Platform for Generating Reproducible Expression Data from Streptomyces coelicolor Batch Cultivations. Adv. Exp. Med. Biol. 696:3-15.
  20. Mast, Y., T. Weber, M. Gölz, R. Ort-Winklbauer, A. Gondran, W. Wohlleben, and E. Schinko. 2011. Characterization of the ‘Pristinamycin Supercluster’ of Streptomyces pristinaespiralis. Microbial Biotechnol. 4:192-206.
  21. Stegmann, E., H.-J. Frasch, and W. Wohlleben. 2010. Glycopeptide biosynthesis in the context of basic cellular functions. Curr. Opinion Microbiol. 5:595-602.
  22. Alam, M.T., M.E. Merlo, STREAM Consortium*, D.A. Hodgson, E.M. Wellington, E. Takano, R. Breitling. 2010. Metabolic modeling and analysis of the metabolic switch in Streptomyces coelicolor. BMC Genomics 11:202. (* incl. W. Wohlleben, J. Reuther)
  23. Thykaer, J., J. Nielsen, W. Wohlleben, T. Weber, M. Gutknecht, A.E. Lantz, and E. Stegmann. 2010. Increased glycopeptide production after overexpression of shikimate pathway genes being part of the balhimycin biosynthetic gene cluster. Metab. Eng. 12:455-461.
  24. Lopez, P., A. Hornung, K. Welzel, C. Unsin, W. Wohlleben, T. Weber, and S. Pelzer. 2010. Isolation of the lysolipin gene cluster of Streptomyces tendae Tü 4042. Gene 461:5-14.
  25. Geib, N., T. Weber, T. Wörtz, K. Zerbe, W. Wohlleben, and J.A. Robinson. 2010. Genome mining in Amycolatopsis balhimycina for ferredoxins capable of supporting cytochrome P450 enzymes involved in glycopeptide antibiotic biosynthesis. FEMS Microbiol Lett. 306:45-53.
  26. Mulyani, S., E. Egel, C. Kittel, S. Turkanovic, W. Wohlleben, R.D. Süssmuth, and K.-H. van Pée. 2010. The thioesterase Bhp is involved in the formation of b-hydroxytyrosine during balhimycin biosynthesis in Amycolatopsis balhimycina. Chembiochem 11:266-271.
  27. Nieselt, K., F. Battke, A. Herbig, P. Bruheim, A. Wentzel, O.M. Jakobsen, H. Sletta, M.T. Alam, M.E. Merlo, J. Moore, W.A. Omara, E.R. Morrisey, M.A. Juarez-Hermosillo, A. Rodriguez-Garcia, M. Nentwich, L. Thomas, M. Iqbal, R. Legaie, W.H. Gaze, G.L. Challis, R.C. Jansen, L. Dijkhuizen, D.A. Rand, D.L. Wild, M. Bonin, J. Reuther, W. Wohlleben, M.C. Smith, N.J. Burroughs, J.F. Martin, D.A. Hodgson, E. Takano, R. Breitling, T.E. Ellingsen, and E.M. Wellington. 2010. The dynamic architecture of the metabolic switch in Streptomyces coelicolor. BMC Genomics 11:10.
  28. Schinko, E., K. Schad, S. Eys, U. Keller, and W. Wohlleben. 2009. Phosphinothricin-tripeptide biosynthesis: An original version of bacterial secondary metabolism? Phytochemistry 70:1787-1800.
  29. Hühns, M., K. Neumann, T. Hausmann, F. Klemke, W. Lockau, U. Kahmann, L. Kopertekh, D. Staiger, E.K. Pistorius, J. Reuther, E. Waldvogel, W. Wohlleben, M. Effmert, H. Junghans, K. Neubauer, U. Kragl, K. Schmidt, J. Schmidtke, and I. Broer. 2009. Tuber-specific cphA expression to enhance cyanophycin production in potatoes. Plant Biotechnol. J. 7:883-898.
  30. Laiple, K., T. Härtner, H.-P. Fiedler, W. Wohlleben, and T. Weber. 2009. The kirromycin gene cluster of Streptomyces collinus Tü 365 codes for an aspartate-alpha-decarboxylase, kirD, which is involved in the biosynthesis of the precursor b-alanine. J. Antibiot. (Tokyo) 62:465-468.
  31. Weber, T., C. Rausch, P. Lopez, I. Hoof, V. Gaykova, D.H. Huson, and W. Wohlleben. 2009. CLUSEAN: A computer-based framework for the automated analysis of bacterial secondary metabolite biosynthetic gene clusters. J. Biotechnol. 140:13-17.
  32. Malm, S., Y. Tiffert, J. Micklinghoff, S. Schultze, I. Joost, I. Weber, S. Horst, B. Ackermann, M. Schmidt, W. Wohlleben, S. Ehlers, R. Geffers, J. Reuther, and F.-C. Bange. 2009. The roles of the nitrate reductase NarGHJI, the nitrite reductase NirBD, and the response regulator GlnR in nitrate assimilation of Mycobacterium tuberculosis. Microbiology 155:1332-1339.
  33. Wohlleben, W., E. Stegmann, and R.D. Süssmuth. 2009. Molecular Genetic Approaches to Analyze Glycopeptide Biosynthesis. Methods Enzymol. 458:459-486.
  34. Chevillotte, M., R. Menges, G. Muth, W. Wohlleben, and E. Stegmann. 2008. A quick and reliable method for monitoring gene expression in actinomycetes. J. Biotechnol. 135:262-265.
  35. Butz, D., T. Schmiederer, B. Hadatsch, W. Wohlleben, T. Weber, and R. Süssmuth. 2008. Module extension of a non-ribosomal peptide synthetase of the glycopeptide antibiotic balhimycin produced by Amycolatopsis balhimycina. Chembiochem 9:1195-1200.
  36. Eys, S., D. Schwartz, W. Wohlleben, and E. Schinko. 2008. Three Thioesterases Are Involved in the Biosynthesis of Phosphinothricin Tripeptide in Streptomyces viridochomogenes Tü494. Antimicrob. Agents Chemother. 52:1686-1696.
  37. Weber, T., K.J. Laiple, E. K. Pross, A. Textor, S. Grond, K. Welzel, S. Pelzer, A. Vente, and W. Wohlleben. 2008. Molecular Analysis of the Kirromycin Biosynthetic Gene Cluster Revealed b-Alanine as Precursor of Pyridone Moiety. Chem. Biol. 15: 175-188.
  38. Tiffert, Y., P. Supra, R. Wurm, W. Wohlleben, R. Wagner and J. Reuther. 2008. The Streptomyces coelicolor GlnR regulon: identification of new GlnR targets and evidence for a central role of GlnR in nitrogen metabolism in actinomycetes. Mol. Microbiol. 67: 861-880.
  39. Menges, R., G. Muth, W. Wohlleben, and E. Stegmann. 2007. The ABC transporter Tba of Amycolatopsis balhymicina is required for efficient export of the glycopeptide antibiotic balhimycin. Appl. Microbiol. Biotechnol. 77:125-134.
  40. Shawky, R.M., O. Puk, A. Wietzorrek, S. Pelzer, E. Takano, W. Wohlleben, and E. Stegmann. 2007. The Border Sequence of the Balhimycin Biosynthesis Gene Cluster from Amycolatopsis balhimycina Contains bbr, Encoding a StrR-Like Pathway-Specific Regulator. J. Mol. Microbiol. Biotechnol. 13:76-88.
  41. Reuther, J. and W. Wohlleben. 2007. Nitrogen metabolism in Streptomyces coelicolor: transcriptional and post-translational regulation. J. Mol. Microbiol. Biotechnol. 12:139-146.
  42. Hadatsch, B., D. Butz, T. Schmiederer, J. Steudle, W. Wohlleben, R. Süssmuth, and E. Stegmann. 2007. The Biosynthesis of Teicoplanin-Type Glycopeptide Antibiotics: Assignment of P450 Mono-Oxygenases to Side Chain Cyclizations of Glycopeptide A47934. Chem. Biol. 14:1078-1089.
  43. Tiffert Y., B. Götz, J. Reuther, W. Wohlleben, and G. Muth. 2007. Conjugative DNA transfer in Streptomyces: SpdB2 involved in the intramycelial spreading of plasmid pSVH1 is an oligomeric integral membrane protein that binds to dsDNA. Microbiology 153:2976-2983.
  44. Rausch, C., I. Hoof, T. Weber, W. Wohlleben, and D. Huson. 2007. Phylogenetic analysis of condensation domains in NRPS sheds light on their functional evolution. BMC Evol. Biol. 7:78.
  45. Hsiao, N.H., J. Soding, D. Linke, C. Lange, C. Hertweck, W. Wohlleben, and E. Takano. 2007. ScbA from Streptomyces coelicolor A3(2) has homology to fatty acid synthases and is able to synthesize gamma-butyrolactones. Microbiology 153:1394-1404.
  46. Müller, C., S. Nolden, P. Gebhardt, E. Heinzelmann, C. Lange, O. Puk, K. Welzel, W. Wohlleben, and D. Schwartz. 2007. Sequencing and analysis of the biosynthetic gene cluster of the lipopeptide antibiotic friulimicin in Actinoplanes friuliensis. Antimicrob. Agents Chemother. 51:1028-1037.
  47. Stegmann, E., C. Rausch, S. Stockert, D. Burkert, and W. Wohlleben. 2006. The small MbtH-like protein encoded by an internal gene of the balhimycin biosynthetic gene cluster is not required for glycopeptide production. FEMS Microbiol. Lett. 262:85-92.
  48. Stegmann, E., S. Pelzer, D. Bischoff, O. Puk, S. Stockert, D. Butz, K. Zerbe, J. Robinson, R.D. Süssmuth, and W. Wohlleben. 2006. Genetic analysis of the balhimycin (vancomycin-type) oxygenase genes. J. Biotechnol. 124:640-653.
  49. Rexer, H.U., T. Schäberle, W. Wohlleben, and A. Engels. 2006. Investigation of the functional properties and regulation of three glutamine synthetase-like genes in Streptomyces coelicolor A3(2). Arch. Microbiol. 186:447-458.
  50. Reuther, J., W. Wohlleben, and G. Muth. 2006. Modular architecture of the conjugative plasmid pSVH1 from Streptomyces venezuelae. Plasmid 55:201-209.
  51. Reuther, J., C. Gekeler, Y. Tiffert, W. Wohlleben, and G. Muth. 2006. Unique conjugation mechanism in mycelial streptomycetes: a DNA-binding ATPase translocates unprocessed plasmid DNA at the hyphal tip. Mol. Microbiol. 61:436-446.
  52. Mazza, P., E.E. Noens, K. Schirner, N. Grantcharova, A. M. Mommaas, H.K. Koerten, G. Muth, K. Flärdh, G.P. van Wezel, and W. Wohlleben. 2006. MreB of Streptomyces coelicolor is not essential for vegetative growth but is required for the integrity of aerial hyphae and spores. Mol. Microbiol. 60:838-852.
  53. Rausch, C., T. Weber, O. Kohlbacher, W. Wohlleben, and D.H. Huson. 2005. Specificity prediction of adenylation domains in nonribosomal peptide synthetases (NRPS) using transductive support vector machines (TSVMs). Nucleic Acids Res. 33:5799-5808.
  54. Heinzelmann, E., S. Berger, C. Müller, T. Härtner, K. Poralla, W. Wohlleben, and D. Schwartz. 2005. An acyl-CoA dehydrogenase is involved in the formation of the Dcis3 double bond in the acyl residue of the lipopeptide antibiotic friulimicin in Actinoplanes friuliensis. Microbiology 151:1963-1974.
  55. Donadio, S., M. Sosio, E. Stegmann, T. Weber, and W. Wohlleben. 2005. Comparative analysis and insights into the evolution of gene clusters for glycopeptide antibiotic biosynthesis. Mol. Genet. Genomics 274:40-50.
  56. Schwartz, D., N. Grammel, E. Heinzelmann, U. Keller, and W. Wohlleben. 2005. Phosphinothricin tripeptide synthetases in Streptomyces viridochromogenes Tu494. Antimicrob. Agents Chemother. 49:4598-4607.
  57. Bischoff, D., B. Bister, M. Bertazzo, V. Pfeifer, E. Stegmann, G. J. Nicholson, S. Keller, S. Pelzer, W. Wohlleben, and R. D. Süssmuth. 2005. The biosynthesis of vancomycin-type glycopeptide antibiotics--a model for oxidative side-chain cross-linking by oxygenases coupled to the action of peptide synthetases. Chembiochem 6:267-272.
  58. Takano, E., H. Kinoshita, V. Mersinias, G. Bucca, G. Hotchkiss, T. Nihira, C.P. Smith, M. Bibb, W. Wohlleben, and K. Chater. 2005. A bacterial hormone (the SCB1) directly controls the expression of a pathway-specific regulatory gene in the cryptic type I polyketide biosynthetic gene cluster of Streptomyces coelicolor. Mol. Microbiol. 56:465-479.
  59. Eustaquio, A.S., B. Gust, S. M. Li, S. Pelzer, W. Wohlleben, K.F. Chater, and L. Heide. 2004. Production of 8'-halogenated and 8'-unsubstituted novobiocin derivatives in genetically engineered Streptomyces coelicolor strains. Chem. Biol. 11:1561-1572.
  60. Schwartz, D., S. Berger, E. Heinzelmann, K. Muschko, K. Welzel, and W. Wohlleben. 2004. Biosynthetic gene cluster of the herbicide phosphinothricin tripeptide from Streptomyces viridochromogenes Tu494. Appl. Environ. Microbiol. 70:7093-7102.
  61. Puk, O., D. Bischoff, C. Kittel, S. Pelzer, S. Weist, E. Stegmann, R.D. Süssmuth, and W. Wohlleben. 2004. Biosynthesis of chloro-b-hydroxytyrosine, a nonproteinogenic amino acid of the peptidic backbone of glycopeptide antibiotics. J. Bacteriol. 186:6093-6100.
  62. Stegmann, E., D. Bischoff, C. Kittel, S. Pelzer, O. Puk, J. Recktenwald, S. Weist, R. Süssmuth, and W. Wohlleben. 2005. Precursor-directed biosynthesis for the generation of novel glycopeptides. Ernst Schering Res Found Workshop. 51:215-232.
  63. Stegmann, E., D. Bischoff, C. Kittel, S. Pelzer, O. Puk, J. Recktenwald, S. Weist, R. Süssmuth, and W. Wohlleben. 2005. Precursor-directed biosynthesis for the generation of novel glycopetides, p. 215-232. In Biocombinatorial Approaches for Drug Finding. W. Wohlleben, T. Spelling, and B. Müller-Thiemann, eds., Springer-Verlag, Berlin.
  64. Weist, S., C. Kittel, D. Bischoff, B. Bister, V. Pfeifer, G.J. Nicholson, W. Wohlleben, and R.D. Süssmuth. 2004. Mutasynthesis of glycopeptide antibiotics: variations of vancomycin's AB-ring amino acid 3,5-dihydroxyphenylglycine. J. Am. Chem. Soc. 126:5942-5943.
  65. Süssmuth, R. D. and W. Wohlleben. 2004. The biosynthesis of glycopeptide antibiotics--a model for complex, non-ribosomally synthesized, peptidic secondary metabolites. Appl. Microbiol. Biotechnol. 63:344-350.
  66. Weber, T., K. Welzel, S. Pelzer, A. Vente, and W. Wohlleben. 2003. Exploiting the genetic potential of polyketide producing streptomycetes. J. Biotechnol. 106:221-232.
  67. Patek, M., G. Muth, and W. Wohlleben. 2003. Function of Corynebacterium glutamicum promoters in Escherichia coli, Streptomyces lividans, and Bacillus subtilis. J. Biotechnol. 104:325-334.
  68. Bister, B., D. Bischoff, G. J. Nicholson, S. Stockert, J. Wink, C. Brunati, S. Donadio, S. Pelzer, W. Wohlleben, and R.D. Süssmuth. 2003. Bromobalhimycin and chlorobromobalhimycins--illuminating the potential of halogenases in glycopeptide antibiotic biosyntheses. Chembiochem 4:658-662.
  69. Heinzelmann, E., S. Berger, O. Puk, B. Reichenstein, W. Wohlleben, and D. Schwartz. 2003. A glutamate mutase is involved in the biosynthesis of the lipopeptide antibiotic friulimicin in Actinoplanes friuliensis. Antimicrob. Agents Chemother. 47:447-457.
  70. Wohlleben, W. and S. Pelzer. 2002. New compounds by combining "modern" genomics and "old-fashioned" mutasynthesis. Chem. Biol. 9:1163-1164.
  71. Zerbe, K., O. Pylypenko, F. Vitali, W. Zhang, S. Rouset, M. Heck, J. W. Vrijbloed, D. Bischoff, B. Bister, R.D. Süssmuth, S. Pelzer, W. Wohlleben, J.A. Robinson, and I. Schlichting. 2002. Crystal structure of OxyB, a cytochrome P450 implicated in an oxidative phenol coupling reaction during vancomycin biosynthesis. J. Biol. Chem. 277:47476-47485.
  72. Muschko, K., G. Kienzlen, H. P. Fiedler, W. Wohlleben, and D. Schwartz. 2002. Tricarboxylic acid cycle aconitase activity during the life cycle of Streptomyces viridochromogenes Tu494. Arch. Microbiol. 178:499-505.
  73. Hoffmeister, D., A. Bechthold, S. Pelzer, and W. Wohlleben. 2002. Herstellung neuer Naturstoffe durch Kombinatorische Biosynthese. Biospektrum Spezialheft:508-509.
  74. Fink, D., N. Weissschuh, J. Reuther, W. Wohlleben, and A. Engels. 2002. Two transcriptional regulators GlnR and GlnRII are involved in regulation of nitrogen metabolism in Streptomyces coelicolor A3(2). Mol. Microbiol. 46:331-347.
  75. Hesketh, A., D. Fink, B. Gust, H.U. Rexer, B. Scheel, K. Chater, W. Wohlleben, and A. Engels. 2002. The GlnD and GlnK homologues of Streptomyces coelicolor A3(2) are functionally dissimilar to their nitrogen regulatory system counterparts from enteric bacteria. Mol. Microbiol. 46:319-330.
  76. Weist, S., B. Bister, O. Puk, D. Bischoff, S. Pelzer, G.J. Nicholson, W. Wohlleben, G. Jung, and R.D. Süssmuth. 2002. Fluorobalhimycin--a new chapter in glycopeptide antibiotic research. Angew. Chem. Int. Ed. Engl. 41:3383-3385.
  77. Recktenwald, J., R. Shawky, O. Puk, F. Pfennig, U. Keller, W. Wohlleben, and S. Pelzer. 2002. Nonribosomal biosynthesis of vancomycin-type antibiotics: a heptapeptide backbone and eight peptide synthetase modules. Microbiology 148:1105-1118.
  78. Puk, O., P. Huber, D. Bischoff, J. Recktenwald, G. Jung, R.D. Süssmuth, K.-H. van Pee, W. Wohlleben, and S. Pelzer. 2002. Glycopeptide biosynthesis in Amycolatopsis mediterranei DSM5908: function of a halogenase and a haloperoxidase/perhydrolase. Chem. Biol. 9:225-235.
  79. Muth, G. and W. Wohlleben. 2001. Homologous Recombination and the Induction of the SOS-Response in Antibiotic Producing Streptomycetes. In Microbial Fundamentals of Biotechnology, Braun, V. and Götz, F. Eds., 151-160. Wiley-VCH, Weinheim.
  80. Pelzer, S. and W. Wohlleben. 2001. Analysis of the Biosynthesis of Glycopeptide Antibiotics: Basis for Creating New Structures by Combinatorial Biosynthesis. In Microbial Fundamentals of Biotechnology, Braun, V. and Götz, F. Eds., 151-160. Wiley-VCH, Weinheim.
  81. Bischoff, D., S. Pelzer, B. Bister, G.J. Nicholson, S. Stockert, M. Schirle, W. Wohlleben, G. Jung, and R.D. Süssmuth. 2001. The Biosynthesis of Vancomycin-Type Glycopeptide Antibiotics-The Order of the Cyclization Steps. Angew. Chem. Int. Ed. Engl. 40:4688-4691. Biosynthese von Glycopeptid-Antibiotika vom Vancomycin-Typ: die Reihenfolge der Ringschlüsse. Angew. Chem. 113:4824-4827.
  82. Stegmann, E., S. Pelzer, K. Wilken, and W. Wohlleben. 2001. Development of three different gene cloning systems for genetic investigation of the new species Amycolatopsis japonicum MG417-CF17, the ethylenediaminedisuccinic acid producer. J. Biotechnol. 92:195-204.
  83. Tünnemann, R., M. Mehlmann, R.D. Sussmuth, B. Buhler, S. Pelzer, W. Wohlleben, H.-P. Fiedler, K.-H. Wiesmüller, G. Gauglitz, and G. Jung. 2001. Optical biosensors. Monitoring studies of glycopeptide antibiotic fermentation using white light interference. Anal. Chem. 73:4313-4318.
  84. Pfeifer, V., G.J. Nicholson, J. Ries, J. Recktenwald, A.B. Schefer, R.M. Shawky, J. Schröder, W. Wohlleben, and S. Pelzer. 2001. A polyketide synthase in glycopeptide biosynthesis: the biosynthesis of the non-proteinogenic amino acid (S)-3,5-dihydroxyphenylglycine. J. Biol. Chem. 276:38370-38377.
  85. Heinzelmann, E., G. Kienzlen, S. Kaspar, J. Recktenwald, W. Wohlleben, and D. Schwartz. 2001. The phosphinomethylmalate isomerase gene pmi, encoding an aconitase-like enzyme, is involved in the synthesis of phosphinothricin tripeptide in Streptomyces viridochromogenes. Appl. Environ. Microbiol. 67:3603-3609.
  86. Vierling, S., T. Weber, W. Wohlleben, and G. Muth. 2001. Evidence that an additional mutation is required to tolerate insertional inactivation of the Streptomyces lividans recA gene. J. Bacteriol. 183:4374-4381.
  87. Bischoff, D., S. Pelzer, A. Höltzel, G.J. Nicholson, S. Stockert, W. Wohlleben, G. Jung, and R.D. Süssmuth. 2001. The Biosynthesis of Vancomycin-Type Glycopeptide Antibiotics-New Insights into the Cyclation Steps. Angew. Chem. Int. Ed. Engl. 40:1693-1696. Die Biosynthese von Glycopeptid-Antibiotika des Vancomycin-Typs - neue Einblicke in die Abfolge der Ringschlüsse. Angew. Chem. 113: 1736-1739.
  88. Weisschuh, N., D. Fink, S. Vierling, M.J. Bibb, W. Wohlleben, and A. Engels. 2000. Transcriptional analysis of the gene for glutamine synthetase II and two upstream genes in Streptomyces coelicolor A3(2). Mol. Gen. Genet. 264:461-469.
  89. Burger, A., K. Sichler, G. Kelemen, M. Buttner, and W. Wohlleben. 2000. Identification and characterization of the mre gene region of Streptomyces coelicolor A3(2). Mol. Gen. Genet. 263:1053-1060.
  90. Vierling, S., T. Weber, W. Wohlleben, and G. Muth. 2000. Transcriptional and mutational analyses of the Streptomyces lividans recX gene and its interference with RecA activity. J. Bacteriol. 182:4005-4011.
  91. Zocher, F., M. M. Enzelberger, U. T. Bornscheuer, B. Hauer, W. Wohlleben, and R.D. Schmid. 2000. Epoxide hydrolase activity of Streptomyces strains. J. Biotechnol. 77:287-292.
  92. Schwartz, D., S. Kaspar, G. Kienzlen, K. Muschko, and W. Wohlleben. 1999. Inactivation of the tricarboxylic acid cycle aconitase gene from Streptomyces viridochromogenes Tu494 impairs morphological and physiological differentiation. J. Bacteriol. 181:7131-7135.
  93. Fink, D., D. Falke, W. Wohlleben, and A. Engels. 1999. Nitrogen metabolism in Streptomyces coelicolor A3(2): modification of glutamine synthetase I by an adenylyltransferase. Microbiology 145:2313-2322.
  94. Süssmuth, R., S. Pelzer, G. J. Nicholson, T. Walk, W. Wohlleben, and G. Jung. 1999. New advances in the biosynthetic gene cluster and its use for manipulating glycopeptide biosynthesis in the producing organism Amycolatopsis mediterranei DSM5908. Angew. Chem. Int. Ed. Engl. 38:1976-1979.
  95. Pelzer, S., R. Süssmuth, D. Heckmann, J. Recktenwald, P. Huber, G. Jung, and W. Wohlleben. 1999. Identification and analysis of the balhimycin biosynthetic gene cluster and its use for manipulating glycopeptide biosynthesis in Amycolatopsis mediterranei DSM5908. Antimicrob. Agents Chemother. 43:1565-1573.
  96. Muth, G., D. F. Brolle, and W. Wohlleben. 1999. Genetics of Streptomyces, p. 353-367. In Manual of Industrial Microbiology and Biotechnology, A. L. Demain and J. E. Davies, eds., ASM Press, Washington D.C.
  97. Süssmuth, R., T. Walk, S. Pelzer, D. Heckmann, W. Wohlleben, and G. Jung. 1999. Structure elucidation of peptide antibiotic balhimycin and biosynthesis intermediates via sequenator-ESI-MS-coupling. p. 296-297, Bajusz, S. and Hudecz, S. eds., Akadémiai Kiadó, Budapest.
  98. Maas, R.M., J. Götz, W. Wohlleben, and G. Muth. 1998. The conjugative plasmid pSG5 from Streptomyces ghanaensis DSM 2932 differs in its transfer functions from other Streptomyces rolling-circle-type plasmids. Microbiology 144:2809-2817.
  99. Lutz-Wahl, S., P. Fischer, C. Schmidt-Dannert, W. Wohlleben, B. Hauer, and R.D. Schmid. 1998. Stereo- and regioselective hydroxylation of alpha-ionone by Streptomyces strains. Appl. Environ. Microbiol. 64:3878-3881.
  100. Schwartz, D., J. Recktenwald, S. Pelzer, and W. Wohlleben. 1998. Isolation and characterization of the PEP-phosphomutase and the phosphonopyruvate decarboxylase genes from the phosphinothricin tripeptide producer Streptomyces viridochromogenes Tu494. FEMS Microbiol. Lett. 163:149-157.
  101. Grammel, N., D. Schwartz, W. Wohlleben, and U. Keller. 1998. Phosphinothricin-tripeptide synthetases from Streptomyces viridochromogenes. Biochemistry 37:1596-1603.
  102. Burger, A., B. Brandt, U. Süsstrunk, C.J. Thompson, and W. Wohlleben. 1998. Analysis of a Streptomyces coelicolor A3(2) locus containing the nucleoside diphosphate kinase (ndk) and folylpolyglutamate synthetase (folC) genes. FEMS Microbiol.Lett. 159:283-291.
  103. Muth, G., D. Frese, A. Kleber, and W. Wohlleben. 1997. Mutational analysis of the Streptomyces lividans recA gene suggests that only mutants with residual activity remain viable. Mol. Gen. Genet. 255:420-428.
  104. Pelzer, S., W. Reichert, M. Huppert, D. Heckmann, and W. Wohlleben. 1997. Cloning and analysis of a peptide synthetase gene of the balhimycin producer Amycolatopsis mediterranei DSM5908 and development of a gene disruption/replacement system. J. Biotechnol. 56:115-128.
  105. Labes, G., M. Bibb, and W. Wohlleben. 1997. Isolation and characterization of a strong promoter element from the Streptomyces ghanaensis phage I19 using the gentamicin resistance gene (aacC1) of Tn1696 as reporter. Microbiology 143:1503-1512.
  106. Subramaniam-Niehaus, B., T. Schneider, J.W. Metzger, and W. Wohlleben. 1997. Isolation and analysis of moenomycin and its biosynthetic intermediates from Streptomyces ghanaensis (ATCC 14672) wildtype and selected mutants. Z. Naturforsch.[C] 52:217-226.
  107. Decker, H., S. Gaisser, S. Pelzer, P. Schneider, L. Westrich, W. Wohlleben, and A. Bechthold. 1996. A general approach for cloning and characterizing dNDP-glucose dehydratase genes from actinomycetes. FEMS Microbiol. Lett. 141:195-201.
  108. Brodbeck, M., A. Rohling, W. Wohlleben, C. Thompson, and U. Süsstrunk. 1996. Nucleoside-diphosphate kinase from Streptomyces coelicolor. Eur. J. Biochem. 239:208-213.
  109. Schwartz, D., R. Alijah, B. Nussbaumer, S. Pelzer, and W. Wohlleben. 1996. The peptide synthetase gene phsA from Streptomyces viridochromogenes is not juxtaposed with other genes involved in nonribosomal biosynthesis of peptides. Appl. Environ. Microbiol. 62:570-577.
  110. Maas, R.M., G. Muth, and I.W.W. Ersfeld. 1995. Involvement of the replication protein of the Streptomyces ghanaensis plasmid pSG5 in conjugative transfer. Genes, Chromosomes, Genomes 3:63.
  111. Wohlleben, W., G. Muth, D. Frese, and B. Nussbaumer. 1994. The Biology of Actinomycetes, p. 75-79. In Proceedings of the Ninth International Symposium on the Biology of Actinomycetes. All-Russia Scientific Research Institute for Genetics and Selection of Industrial Microorganisms, V. G. Debabov, Y. V. Dudnik, and V. N. Danilenko, eds., Moskau.
  112. Muth, G., M. Farr, V. Hartmann, and W. Wohlleben. 1995. Streptomyces ghanaensis plasmid pSG5: nucleotide sequence analysis of the self-transmissible minimal replicon and characterization of the replication mode. Plasmid 33:113-126.
  113. Roth, M., C. Hoffmeier, R. Geuther, G. Muth, and W. Wohlleben. 1994. Segregational stability of pSG5-derived vector plasmids in continous cultures of Streptomyces lividans 66. Biotechnol. Lett. 16:1225-1230.
  114. Nussbaumer, B. and W. Wohlleben. 1994. Identification, isolation and sequencing of the recA gene of Streptomyces lividans TK24. FEMS Microbiol. Lett. 118:57-63.
  115. Wohlleben, W., V. Hartmann, D. Hillemann, K. Krey, G. Muth, B. Nussbaumer, and S. Pelzer. 1994. Transfer and establishment of DNA in Streptomyces (a brief review). Acta Microbiol. Immunol. Hung. 41:381-389.
  116. Wohlleben, W. and G. Muth. 1993. Streptomyces plasmid vectors, p. 147-175. In Plasmids: A Practical Approach. K. G. Hardy, ed., Oxford University Press, Oxford.
  117. Wohlleben, W., G. Muth, and J. Kalinowski. 1993. Genetic Engineering of Microorganisms, p. 83-133. In Genetic Engineering of Microorganisms. A. Pühler, ed., Verlag Chemie, Weinheim.
  118. Hillemann, D., T. Dammann, A. Hillemann, and W. Wohlleben. 1993. Genetic and biochemical characterization of the two glutamine synthetases GSI and GSII of the phosphinothricyl-alanyl-alanine producer, Streptomyces viridochromogenes Tu494. J. Gen. Microbiol. 139:1773-1783.
  119. Kumada, Y., D. R. Benson, D. Hillemann, T. J. Hosted, D. A. Rochefort, C. J. Thompson, W. Wohlleben, and Y. Tateno. 1993. Evolution of the glutamine synthetase gene, one of the oldest existing and functioning genes. Proc. Natl. Acad. Sci. U.S.A 90:3009-3013.
  120. Wohlleben, W., G. Muth, and J. Kalinowski. 1993. Genetic Engineering of Gram Positive Bacteria., p. 455-505. In H.-J. R. G. Rehm, ed., Biotechnology. Verlag Chemie, Weinheim.
  121. Jäger, W., A. Schäfer, A. Pühler, G. Labes, and W. Wohlleben. 1992. Expression of the Bacillus subtilis sacB gene leads to sucrose sensitivity in the gram-positive bacterium Corynebacterium glutamicum but not in Streptomyces lividans. J. Bacteriol. 174:5462-5465.
  122. Dammann, T. and W. Wohlleben. 1992. A metalloprotease gene from Streptomyces coelicolor 'Muller' and its transcriptional activator, a member of the LysR family. Mol. Microbiol. 6:2267-2278.
  123. Wohlleben, W., R. Alijah, J. Dorendorf, D. Hillemann, B. Nussbaumer, and S. Pelzer. 1992. Identification and characterization of phosphinothricin-tripeptide biosynthetic genes in Streptomyces viridochromogenes. Gene 115:127-132.
  124. Wohlleben, W., W. Arnold, I. Behrmann, I. Broer, A. Hillemann, A. Pühler, and E. Strauch. 1991. Genetic Analysis of Different Resistance Mechanisms Against the Herbicidal Antibiotic Phosphinothricyl-Alanyl-Alaine., p. 171-183. In Genetics and Product Formation in Streptomyces. S.K.H.N.D. Baumberg, ed., Plenum Press, London.
  125. Alijah, R., J. Dorendorf, S. Talay, A. Pühler, and W. Wohlleben. 1991. Genetic analysis of the phosphinothricin-tripeptide biosynthetic pathway of Streptomyces viridochromogenes Tu494. Appl. Microbiol. Biotechnol. 34:749-755.
  126. Hillemann, D., A. Pühler, and W. Wohlleben. 1991. Gene disruption and gene replacement in Streptomyces via single stranded DNA transformation of integration vectors. Nucleic Acids Res. 19:727-731.
  127. Bräu, B., R. Hilgenfeld, M. Schlingmann, R. Marquardt, E. Birr, W. Wohlleben, K. Aufderheide, and A. Pühler. 1991. Increased yield of a lysozyme after self-cloning of the gene in Streptomyces coelicolor "Muller". Appl. Microbiol. Biotechnol. 34:481-487.
  128. Behrmann, I., D. Hillemann, A. Pühler, E. Strauch, and W. Wohlleben. 1990. Overexpression of a Streptomyces viridochromogenes gene (glnII) encoding a glutamine synthetase similar to those of eucaryotes confers resistance against the antibiotic phosphinothricyl-alanyl-alanine. J. Bacteriol. 172:5326-5334.
  129. Labes, G., R. Simon, and W. Wohlleben. 1990. A rapid method for the analysis of plasmid content and copy number in various Streptomycetes grown on agar plates. Nucleic Acids Res. 18:2197.
  130. Wohlleben, W., G. Muth, B. Nussbaumer, A. Hillemann, and A. Pühler. 1990. Suicide Vector Systems for Streptomycetes. J. Cell. Biochem. 14A:131.
  131. Birr, E., W. Wohlleben, A. Pühler, B. Bräu, and R. Marquardt. 1990. Cloning and Characterisation of a Lysozyme Gene from Streptomyces coelicolor "Müller" DSM3030. J. Cell. Biochemistry 14A:105.
  132. Muth, G., B. Nussbaumer, W. Wohlleben, and A. Pühler. 1989. A vector system with temperature-sensitive replication for gene disruption and mutational cloning in Streptomycetes. Mol. Gen. Genet. 219:341-348.
  133. Wohlleben, W., and A. Pielsticker. 1989. Investigation of Plasmid Transfer between Escherichia coli and Streptomyces lividans. pp. 301-305. In DECHEMA Biotechnology Conference Bd. 3, Behrens, D. Driesel A. eds., Weinheim, VCH Verlagsgesellschaft.
  134. Wohlleben, W., W. Arnold, L. Bissonnette, A. Pelletier, A. Tanguay, P.H. Roy, G.C. Gamboa, G. F. Barry, E. Aubert, J. Davies, and S.A. Kagan. 1989. On the evolution of Tn21 -like multiresistance transposons: sequence analysis of the gene (aacC1) for gentamicin acetyltransferase-3-I(AAC(3)-I), another member of the Tn21-based expression cassette. Mol. Gen. Genet. 217:202-208.
  135. Broer, I., W. Arnold, W. Wohlleben, and A. Pühler. 1989. The Phosphinothricin N-Acetyltransferase Gene as a Selection Marker for Plant Genetic Engineering. pp. 240-246. In Proc Braunschweig Symp on Applied molecular biology, G. Galling, ed., Braunschweig, Zentralstelle für Weiterbildung, Braunschweig.
  136. Birr, E., W. Wohlleben, K. Aufderheide, T. Schneider, A. Pühler, B. Brau, R. Marquardt, and M. Schlingmann. 1989. Isolation and complementation of mutants of Streptomyces coelicolor "Müller" DSM3030 deficient in lysozyme production. Appl. Microbiol. Biotechnol. 30:358-363.
  137. Wohlleben, W., W. Arnold, I. Broer, D. Hillemann, E. Strauch, and A. Pühler. 1988. Nucleotide sequence of the phosphinothricin N-acetyltransferase gene from Streptomyces viridochromogenes Tu494 and its expression in Nicotiana tabacum. Gene 70:25-37.
  138. Muth, G., W. Wohlleben, and A. Pühler. The minimal replicon of the Streptomyces ghanaensis plasmid pSG5 identified by subcloning and Tn5 mutagenesis. Mol. Gen. Genet. 211: 424-429. 1988.
  139. Strauch, E., W. Wohlleben, and A. Pühler. 1988. Cloning of a phosphinothricin N-acetyltransferase gene from Streptomyces viridochromogenes Tu494 and its expression in Streptomyces lividans and Escherichia coli. Gene 63:65-74.
  140. Strauch, E., W. Wohlleben, and A. Pühler. 1987. Development of a plasmid-cloning system for Streptomyces viridochromogenes Tu494. J. Basic Microbiol. 27:449-455.
  141. Wohlleben, W. and A. Pühler . 1987. The Streptomyces ghanaensis low copy plasmid pSG2 and its use for vector construction. Arch. Microbiol. 148:298-304.
  142. Wohlleben, W., G. Muth, E. Birr, and A. Pühler. A vector system for cloning in Streptomyces and Escherichia coli. Szabó, G. Biro S. Goodfellow M. 6, 99-101. 1986. Budapest, Akadémiai Kiadó. Sixth Int. Symp. on Actinomycetes Biology.
  143. Wohlleben, W. and A. Pühler. 1984. Development of Shuttle Vectors between Streptomyces Species and Escherichia coli., p. 219-224. In European Federation of Biotechnology (ed.), Third European Congress on Biotechnology. Verlag Chemie, Weinheim.
  144. Anne, J., W. Wohlleben, H. J. Burkardt, R. Springer, and A. Pühler. 1984. Morphological and molecular characterization of several actinophages isolated from soil which lyse Streptomyces cattleya or S. venezuelae. J.Gen. Microbiol. 130:2639-2649.
  145. Lotz, W., H. Fees, W. Wohlleben, and H. J. Burkhardt. 1981. Isolation and Characterization of the DNA-dependent RNA Polymerase of Rhizobium leguminosarum 300. J. Gen. Microbiol. 125:301-309.
  146. Burkardt, H. J. and W. Wohlleben. 1981. RNA polymerase binding sites on the broad host range plasmid RP4. J. Gen. Microbiol. 125:189-193.
  147. Burkardt, H. J., A. Pühler, and W. Wohlleben. 1980. Adenine + thymine content of different genes located on the broad host range plasmid RP4. J. Gen. Microbiol. 117:135-140.
  148. Pühler, A., H. J. Burkardt, F.C. Cannon, and W. Wohlleben. 1979. Spontaneous degradation of pRD1 DNA into unique size classes is recA dependent. Mol. Gen. Genet. 171:1-6.