Zentrum für Molekularbiologie der Pflanzen (ZMBP)

Central Facilities - ZMBP Microscopy

more information

Location

Auf der Morgenstelle 32, floor 3

  Room # Tel. #
Office Stierhof 3U09 29-76662
Office Richter 6X07
5X13
3U09		 29-78665
29-78098
29-76662
Office Krieger

6X07

5X13

 29-78665
29-78098
Office Stahl 6X07
3W08
3U09		 29-78665
29-76663
29-76662
Lab 3W08 29-76663
Ultramicrotomy 3U08 29-78087
TEM Jeol 1400plus 3W04 29-76661
SEM Hitachi S-800 3Y01 29-78088
Confocal Leica TCS SP8, upright 3U01 29-78808

Confocal Leica TCS SP8 AOBS-FLIM-FCS, inverted

3R01

 29-78860
Confocal Zeiss LSM 880 Airyscan (fast), inverted 3V01 29-77466

EM preparation equipment

 3U05  
Photo camera (high resolution/high quality) for plants, agar plates, gels etc (with incident light and transmitted light illumination; tripod etc) (you will find the camera in the darkroom; please pick up the key in the EM lab) 3W06  

Techniques provided include:

  • Negative Staining
  • Glycerol Spraying
  • Heavy Metal Coating/Carbon Coating
  • Sputter Coating
  • Freeze-Drying
  • Freeze Fracture
  • Critical-Point Drying
  • Cryoimmobilisation (High Pressure Freezing (MPI für Entwicklungsbiologie), Propan Jet Freezing, Plunge Freezing)
  • Freeze-Substitution
  • Resin Embedding (Spurr, Epon)
  • (Low Temperature) Embedding (LR White/Gold, Lowicryls HM20, K11M)
  • Technovit Embedding
  • Microwave fixation and microwave resin embedding
  • Ultrathin Resin Sectioning
  • Ultrathin Tokuyasu Cryosectioning for Immunolabelling
  • Resin Section and Cryosection Immunofluorescence and Immunogold Labelling, Pre-embedding Immunogold Labelling
  • Correlative light and electron microscopy CLEM
  • Confocal Light Microscopy

 

Equipment:

  • Transmission Electron Microscope JEOL 120 kV 1400plus with Tietz TemCam F-416 CMOS camera
  • Scanning Electron Microscope Hitachi S-800 with BSE detector
  • Epi-fluorescence microscope Zeiss Imager M2 with Hamamatsu ORCA-flash 4.0 V.2 CMOS camera
  • Evaporation and Freeze Fracture Units Balzers BAF 300 and 400T
  • Critical-Point Dryer
  • Sputter Coater
  • Glow Discharge Unit
  • Microwave Pelco Biowave Pro
  • Freeze-Substitution and Low Temperature Embedding Unit Leica EMAFS and EMAFS2
  • Ultramicrotomes Leica UCT, Reichert Ultracut E, LKB Ultrotome III
  • Cryoultramikrotome Leica UCT/EMFCS
  • Cryoultramicrotome Leica UC7/FC7
  • Pyramitome LKB
  • Confocal Microscope Leica TCS SP8 upright (Laser lines 405,458,476,488,496,514,561,594,633)
  • Confocal Microscope Leica TCS SP8 (Laser lines: diode 405, pulsed 440, pulsed 470, Argon 488,496,514, DPSS 561, HeNe 594, HeNe 633) eqipped with resonant scanner, HyD detectors and FLIM & FCS
  • Confocal Microscope Zeiss LSM 880 Airyscan
  • Photo camera (high resolution) for plants, agar plates gels etc with equipment (you will find the camera in the darkroom (3W06), key in the EM lab), instruction by EM staff required


TEM Jeol 120 kV 1400plus

SEM Hitachi S800

 

Epifluorescence microscope Zeiss Imager M.2

Balzers BAF 300 and BAF 400

Pyramitome LKB, ultramicrotome Reichert Ultracut E and cryo-ultramicrotome Leica UCT

CLSM Leica SP8 AOBS

CLSM Leica SP8 AOBS with FLIM and FCS unit

 

Service provided:

  • Introduction in use of equipment
  • Assistance in preparation of samples
  • Help with designing experiments
  • Collaboration (for external users please contact Dr. York-Dieter Stierhof)
  • Central storage of embedded samples and TEM grids
  • Development, printing, digitization and archiving of negatives

The unit provides gold markers of different sizes and specificity, fluorescent markers, silver enhancers, fixatives, embedding resins (Spurr, Epon, LR White, LR Gold, Lowicryls HM20, K4M, K11M, Technovit), coated TEM grids, diamond knives for resin and cryosectioning, heavy metal stains, a number of light microscopic stains (see below) etc.


 

Confocal Laser Scanning Microscopes

a)

Microscope:

Leica SP8 AOBS (inverted)

Location:

ZMBP microscopy, floor 3, microscopy, room 3R01
Tel: 29-78860

Technical parameters:
  • AOBS
  • Leica DM RE microscope (inverted) with 100 W mercury lamp for epifluorescence microscopy
  • Resonant scanner
  • 3 HyD detectors, 1 photomultiplier
  • FLIM & FC(C)S unit (Picoquant)
  • Equipped with an Argon laser (458, 488, 514 nm), diode laser (405 nm), DPSS laser (561 nm), HeNe lasers (594 nm, 633 nm), pulsed lasers (440, 470 nm)
  • Leica Lightening deconvolution software
  • Connected to server for data transfer

Coordinator:

Sandra Richter (responsible). Phone during Corona period: 29-78665 (not from outside), or 29-78098.
Natalie Krieger (main contact). Phone during Corona period: 29-78098, or 29-78665 (not from outside)

b)

Microscope:

Zeiss LSM880 Airyscan (fast) (inverted)

Location:

ZMBP microscopy, floor 3, microscopy, room 3V01
Tel: 29-77466

Technical parameters:
  • Zeiss with 100 W mercury lamp for epifluorescence microscopy
  • Equipped with an Argon laser (458, 488, 514 nm), diode laser (405 nm), DPSS laser (561 nm), HeNe lasers (594 nm, 633 nm)
  • Superresolution Airyscan + Airyscan fast
  • Spectral detector for linear unmixing
  • GaAsP + 2 PMT detectors, Airyscan detector
  • Connected to server for data transfer

Coordinator:

Sandra Richter (responsible). Phone during Corona period: 29-78665 (not from outside), or 29-78098.
Natalie Krieger (main contact). Phone during Corona period: 29-78098, or 29-78665 (not from outside)

 

c)

Microscope:

Leica TCS SP8 (upright)

Location:

ZMBP microscopy, floor 3, microscopy, room 3U01
Tel: 29-78808

Technical parameters:
  • Leica DM RE microscope (upright) connected to a PC computer (Windows XP) with 50W mercury lamp for epifluorescence
  • Beamsplitter-based system
  • Equipped with  solid state lasers (448, 488, 514, 552 nm), an UV-diode laser (405 nm).
  • 1 dip-in objective
  • Hygens deconvolution software
  • Connected to server for data transfer

Coordinator:

 Sandra Richter (responsible). Phone during Corona period: 29-78665 (not from outside), or 29-78098.
Natalie Krieger (main contact). Phone during Corona period: 29-78098, or 29-78665 (not from outside)

User Rules and Responsibilities:

  1. Need to be introduced before using the CLSM.
  2. Inform Natalie about any problems with the Leica machines and about problems with the Zeiss confocal.
  3. Save temporary your data on volume D:/, and remove your data from the computer.

Service provided by Natalie/Sandra (Leica SP8):

  1. Contact with technical service of Leica.
  2. Instruction in use and answer to the questions when it doesn't work.
  3. For external users please contact Dr. Sandra Richter

Service provided by Natalie/Sandra (Zeiss LSM880):

  1. Contact with technical service of Zeiss.
  2. Instruction in use and answer to the questions when it doesn't work.
  3. For external users please contact Dr. Sandra Richter

 

List of LM Stains

     

Farbstoff

Firma / Artikelnr.

Menge

8-Hydroxychinolin

Merck / 7098

25 g

Alcianblau

Schuchardt / Al 220

25 g

Alcianblau 8 GS

Fluka / 05500

10 g

Amidoscharz 10 B

Merck / 1167

25 g

Anilinblau

  

0,5 g

Anilinblau WS

Merck / 1275

25 g

Azur I

Chroma / 10155

10 g

Bromkresolgrün

Merck / 8121

1 g

Bromthymolblau

Merck / 3026

1 g

Carmin

Merck / 2233

25 g

Chlorazol Azurine

ICN / 12222

10 g

Chlorzinkjod-Lsg.

Merck / 9276

50 ml

Coomassie Brilliant Blue

  

50 g

Einschlußmittel W 15

Carl Zeiss

25 ml

Eosin bläulich

    

Eosin gelblich

Merck / 1343

25 g

Etzold`s

Chroma / 2C 275

250 ml

Flavognost

Carl Roth

10 g

Fuchsin basisch

Serva / 21915

25 g

Giemsas

Merck / 9204

500 ml

Haemin (chlorid)

Roth / 7629

1 g

Hexadecen

    

Janusgrün

Merck / 1324

5 g

Jod-Jod Kali Lsg.

  

50 ml

Kalium ferricyanatum

Merck / 722196

250 g

Kaliumcyanoferrat II

Riedel / 30447

25 g

Lanthannitrat

Riedel / 31617

2 g

Lichtgrün gelblich

Merck / 1315

25 g

Malachitgrün

Merck / 1398

25 g

May-Grünwald

  

10 ml

Methyblau

Merck

25 g

Methylenblau / HCl

Serva / 29198

25 g

Methylenblau B

Merck / 1283

25 g

Methylrot

Merck / 6076

250 g

Methylrot

Merck / 6076

4 g

Naphtholgelb S

Fluka / 70540

100 g

Neutralrot

Merck / 1370

25 g

Neutralrot

Chroma 1 B 469

25 g

Neutralrot

Merck / 1369

25 g

Neutralrot Lösung

  

20 ml

Neutralviolett

ICN

2 g

Nigrosin

Merck / 1.15924.0025

25 g

Nilblau A

Fluka / 72480

10g

Nile Blue chlorid

Fluka / 72482

5 g

O-Dianisidine

Merck / 2953

25 g

Oil Red O

Serva 7 31170

1 g

Ölrot

Fluka / 75087

25 g

Orange G

Merck / 6878

50 g

Orcein

Merck / 1.07100.0005

5 g

Orcein

Merck / 7091

1 g

Pararosanilin

Merck / 7601

25 g

Phenolrot

Merck / 7241

25 g

Phenolrot

Merck / 7241

5 g

Phloroglucin

  

30 ml

Phloroglucin/HCl 15%

Roth / 54411

10 g

p-Nitrophenol

Sigma / 104-8

5 g

p-Nitrotetrazolium Blau Grade III

Sigma / N-6876

1 g

Pontacylarmin 2 B

/ 113049

10 g

Prune pure

Chroma

10 g

Pyronin G

Merck / 7517

5 g

Reinecke-Salz

Riedel / 33601

20 g

Reinecke-Salz

Riedel / 33601

20 g

Rhodamin B

Merck / 7599

25 g

Safranin T

Riedel / 32610

25 g

Sudan- Glycerin Lsg.

  

20 ml

Sudan III

Fluka / 86005

25 g

Sudan III

Merck / 1380

25 g

Sudan III

Merck / 1380

25 g

Sudan Rot B

    
Thymolblau CB Merck 25 g
Toluidinblau O Merck / 1273 50 g
Toluidinblau O Serva / 36692 25 g
Trypanblau Serva 7 37252 10 g