Zentrum für Molekularbiologie der Pflanzen (ZMBP)

Research Group Schaaf

Signaling by lipid transfer proteins and inositol-derived molecules / Molecular plant nutrition



Laha D, Parvin N, Dynowski M, Johnen P, Mao H, Bitters ST, Zheng N, Schaaf G (2016).
Inositol polyphosphate binding specificity of the jasmonate receptor complex.
Plant Physiol DOI:10.1104/pp.16.00694

Pavlovic I, Thakor DT, Bigler L, Wilson MSC, Laha D, Schaaf G, Saiardi A, Jessen HJ (2015).
Pro-Metabolites of 5-diphospho-myo-inositol pentakisphosphate.
Angew Chem Int Ed 54, 9622-6

Laha D, Johnen P, Azevedo C, Dynowski M, Weiß M, Capolicchio S, Mao H, Iven T, Steenbergen M, Freyer M, Gaugler P, de Campos MKF, Zheng N, Feussner I, Jessen HJ, Van Wees SC, Saiardi A, and Schaaf G (2015).
VIH2 Regulates the Synthesis of Inositol Pyrophosphate InsP8 and Jasmonate-Dependent Defenses in Arabidopsis.
Plant Cell 27, 1082-97

Ghosh R#, de Campos MK#, Huang J, Huh SK, Orlowski A, Yang Y, Tripathi A, Nile A, Lee HC, Dynowski M, Schäfer H, Róg T, Lete MG, Ahyayauch H, Alonso A, Vattulainen I, Igumenova TI, Schaaf G*, Bankaitis VA* (2015).
Sec14-Nodulin Proteins and the Patterning of Phosphoinositide Landmarks for Developmental Control of Membrane Morphogenesis.
Mol Biol Cell 26, 1764-81 (#equally contributing first authors, *co-corresponding authors)

Bankaitis VA, Ile KE, Nile AH, Ren J, Ghosh R, Schaaf G (2012)
Thoughts on Sec14-like nanoreactors and phosphoinositide signaling.
Adv Biol Regul 52, 115-21

Winklbauer EM, de Campos MKF, Dynowski D, and Schaaf G (2011).
A blueprint for functional engineering: Single point mutations reconstitute phosphatidylinositol presentation in a pseudo-Sec14 protein.
Commun Integr Biol 4, 674-8

Ren J, Schaaf G, Bankaitis VA, Ortlund EA, and Pathak MC (2011)
Crystallization and Preliminary X-ray Diffraction Analysis of Sfh3, a Member of the Sec14-protein Superfamily.
Acta Cryst F 67, 1239-1243

Schaaf G*, Dynowski D, Mousley CJ, Shah SD, Yuan P, Winklbauer EM, Kaphan Freitas de Campos M, Trettin K, Quinones MC, Smirnova T, Yanagisawa LL, Ortlund EA, and Bankaitis VA* (2011)
Resurrection of a functional phosphatidylinositol transfer protein from a pseudo-Sec14 scaffold by directed evolution.
Mol Biol Cell 22, 892-905 (* co-corresponding authors)

Mousley CJ, Trettin KD, Tyeryar K, Ile KE, Schaaf G, Bankaitis VA (2010)
Sphingolipid metabolism in trans-Golgi/endosomal membranes and the regulation of intracellular homeostatic processes in eukaryotic cells.
Adv Enzyme Regul 50, 339-48

Bankaitis VA, Mousley CJ, Schaaf G (2010)
The Sec14 superfamily and mechanisms for cross-talk between lipid metabolism and lipid signaling.
Trends Biochem Sci 35, 150-60

Mousley CJ, Tyeryar KR, Ile KE, Schaaf G, Brost RL, Boone C, Guan X, Wenk MR, Bankaitis VA (2008)
Coordinate defects in Sec14 and Tlg2-dependent trans-Golgi and endosome dynamics derange ceramide homeostasis and compromise the unfolded protein response.
Mol Biol Cell 19, 4785-803

Dynowski M*, Schaaf G*, Loque D, Moran O, Ludewig U (2008)
Plant plasma membrane water channels conduct the signaling molecule H2O2.
Biochem J 414, 53-61

Schaaf G, Ortlund EA, Tyeryar KR, Mousley CJ, Ile KE, Garrett TA, Ren J, Woolls MJ, Raetz CRH, Redinbo MR, Bankaitis VA (2008)
Functional Anatomy of Phospholipid Binding and Regulation of Phosphoinositide Homeostasis by Proteins of the Sec14 Superfamily.
Mol Cell 29, 191-206

Smirnova TI, , Chadwick TG, Voinov MA, Poluektov O, van Tol J, Ozarowski A, Schaaf G, Ryan MM, and Bankaitis VA (2007).
Local Polarity and Hydrogen Bonding inside the Sec14p Phospholipid-binding Cavity: High-Field Multifrequency EPR Studies.
Biophysical J 92, 3686-95

Meda AR, Scheuermann E, Prechsl U, Erenoglu B, Schaaf G, Hayen H, Weber G, and von Wirén N (2007)
Iron Acquisition by Phytosiderophores Contributes to Cadmium Tolerance.
Plant Physiol 143, 1761-73

Smirnova TI, Chadwick TG, MacArthur R, Poluektov O, Song L, Ryan MM, Schaaf G, and Bankaitis VA (2006)
The chemistry of phospholipid binding by the Saccharomyces cerevisiae phosphatidylinositol transfer protein Sec14p as determined by EPR spectroscopy.
J Biol Chem 281, 34897-908

Schaaf G, Betts L, Garrett TA, Raetz CRH and Bankaitis VA (2006)
Crystallization and preliminary X-ray diffraction analysis of Sfh1p: A member of the Saccharomyces cerevisea Sec14p-like phosphatidylinositol transfer protein family.
Acta Cryst F 62, 1156-60

Ile KE, Schaaf G, and Bankaitis VA (2006)
Phosphatidylinositol transfer proteins and cellular nanoreactors for lipid signaling.
Nature Chem Biol 2, 576-583

Schaaf G, Honsbein A, Meda AR, Kirchner S, Wipf D and von Wirén N (2006)
AtIREG2 Encodes a Tonoplast Transport Protein Involved in Fe-Dependent Ni Detoxification in Arabidopsis thaliana Roots.
J Biol Chem 281, 25532-25540

Takano J, Wada M, Ludewig U, Schaaf G, von Wirén N, Fujiwara T (2006)
The Arabidopsis Major Intrinsic Protein NIP5;1 Is Essential for Efficient Boron Uptake and Plant Development under Boron Limitation.
Plant Cell 18, 1498 - 1509

Mayer M, Schaaf G, Mouro I, Lopez C, Colin Y, Neumann P, Cartron JP, Ludewig U (2006)
Different Transport Mechanisms in Plant and Human AMT/Rh-type Ammonium Transporters.
J Gen Physiol 127, 133-44

Phillips SE, Vincent P, Rizzieri KE, Schaaf G, Gaucher EA, Bankaitis VA (2006)
The diverse biological functions of phosphatidylinositol transfer proteins in eukaryotes.
Crit Rev Biochem Mol Biol 41, 21-49

Schaaf G, Schikora A, Häberle J, Vert G, Ludewig U, Briat JF, Curie C and von Wirén N (2005)
A putative function of the Arabidopsis Fe-phytosiderophore transporter homolog AtYSL2 in Fe and Zn homeostasis.
Plant Cell Physiol 46, 762-774

Römheld V and Schaaf G (2004)
Iron transport in plants: Future research in view of a plant nutritionist and a molecular biologist.
Soil Sci Plant Nutr 50, 1003 - 1012

Schaaf G, Erenoglu BE and von Wirén N (2004)
Physiological and biochemical characterization of metal-phytosiderophore transport in graminaceous species.
Soil Sci Plant Nutr 50, 955 - 964

Schaaf G, Ludewig U, Erenoglu BE, Mori S, Kitahara T, von Wirén N (2004)
ZmYS1 functions as a proton-coupled symporter for phytosiderophore- and nicotianamine-chelated metals.
J Biol Chem 279, 9091-9096

Schaaf G, Catoni E, Fitz M, Schwacke R, Schneider A, von Wirén N, Frommer WB (2002)
A putative role for the vacuolar calcium/manganese proton antiporter AtCAX2 in heavy metal detoxification.
Plant Biology 4, 612-618

Laufer G*, Schaaf G*, Bollgönn S, Günzl A (1999)
In vitro
analysis of alpha-amanitin-resistant transcription from the rRNA, procyclic acidic repetitive protein, and variant surface glycoprotein gene promoters in Trypanosoma brucei. Mol Cell Biol 19, 5466-5473

Dupré E, Schaaf G (1996)
Influence of ions on the unfolding of the spermatozoa of the rock shrimp, Rhynchocinetes typus.
J Exp Zool 274, 358-364


Schaaf G (2004)
Molecular and Biochemical Characterization of Plant Transporters Involved in the Cellular Homeostasis of Mn and Fe (Dissertation).
Der Andere Verlag, Osnabrück, Germany ISBN 3899592085