Zentrum für Molekularbiologie der Pflanzen (ZMBP)

Research Group Schaaf

Signaling by lipid transfer proteins and inositol-derived molecules / Molecular plant nutrition

Prof. Dr. Gabriel Schaaf

Universität Bonn
INRES - Institut für Nutzpflanzenwissenschaften und Ressourcenschutz
Bereich Pflanzenernährung
Karlrobert-Kreiten-Strasse 13
D-53115 Bonn

Email: gabriel.schaafspam prevention@zmbp.uni-tuebingen.de
Tel: (+49) 228 73-2851
Fax: (+49) 228 73-2489

Universität Tübingen
ZMBP, Pflanzenphysiologie
Auf der Morgenstelle 32
D-72076 Tübingen / Germany
e-mail: gabriel.schaaf(a)zmbp.uni-tuebingen.de
Tel.: +49 7071 29 7 88 82
Fax: +49 (0)7071 - 29 32 87


Our lab uses biochemistry, genetics, chemical-, molecular-, and synthetic biology as well as structural approaches to investigate the molecular mechanisms by which plants perceive different abiotic and biotic signals and integrate diverse external stimuli into meaningful responses. We are especially interested in the roles of membrane lipids, in particular phosphoinositides and phosphoinositide-derived soluble inositol phosphates in membrane morphogenesis, developmental programs, hormone perception, nutrient homeostasis, and the generation of stress responses. Our goal is that our discoveries in Arabidopsis and baker’s yeast will help to clarify signaling roles of inositol-derived molecules and have translational value for crop breeding and biotechnology.

The following projects are ongoing:

Selected publications click here for all publications

Original work:

Laha D, Johnen P, Azevedo C, Dynowski M, Weiß M, Capolicchio S, Mao H, Iven T, Steenbergen M, Freyer M, Gaugler P, de Campos MKF, Zhen N, Feussner I, Jessen HJ, Van Wees SC, Saiardi A, and Schaaf G (2015)

VIH2 Regulates the Synthesis of Inositol Pyrophosphate InsP8 and Jasmonate-Dependent Defenses in Arabidopsis.

Plant Cell 27, 1082-97

Pavlovic I, Thakor DT, Bigler L, Wilson MSC, Laha D, Schaaf G, Saiardi A, Jessen HJ (2015)

Pro-Metabolites of 5-diphospho-myo-inositol pentakisphosphate.

Angew Chem Int Ed 54, 9622-6

Ghosh R*, de Campos MK*, Huang J, Huh SK, Orlowski A, Yang Y, Tripathi A, Nile A, Lee HC, Dynowski M, Schäfer H, Róg T, Lete MG, Ahyayauch H, Alonso A, Vattulainen I, Igumenova TI, Schaaf G#, Bankaitis VA# (2015)

Sec14-Nodulin Proteins and the Patterning of Phosphoinositide Landmarks for Developmental Control of Membrane Morphogenesis.

Mol Biol Cell 26, 1764-81 (*equally contributing first authors, #shared correspondence)

Schaaf G#, Dynowski D, Mousley CJ, Shah SD, Yuan P, Winklbauer EM, De Campos MKF, Trettin K, Quinones MC, Smirnova T, Yanagisawa LL, Ortlund EA, and Bankaitis VA# (2011)

Resurrection of a functional phosphatidylinositol transfer protein from a pseudo-Sec14 scaffold by directed evolution.typo3/

Mol Biol Cell 22, 892-905 (#shared correspondence)

Schaaf G, Ortlund EA, Tyeryar KR, Mousley CJ, Ile KE, Garrett TA, Ren J, Woolls MJ, Raetz CRH, Redinbo MR, Bankaitis VA (2008)

Functional Anatomy of Phospholipid Binding and Regulation of Phosphoinositide Homeostasis by Proteins of the Sec14 Superfamily.typo3/

Mol Cell 29, 191-206

Schaaf G, Honsbein A, Meda AR, Kirchner S, Wipf D and von Wirén N (2006)

AtIREG2 Encodes a Tonoplast Transport Protein Involved in Fe-Dependent Ni Detoxification in Arabidopsis thaliana Roots.typo3/

J Biol Chem 281, 25532-25540

Schaaf G, Schikora A, Häberle J, Vert G, Ludewig U, Briat JF, Curie C and von Wirén N (2005)
A putative function of the Arabidopsis Fe-phytosiderophore transporter homolog AtYSL2 in Fe and Zn homeostasis.

Plant Cell Physiol 46, 762-774

Schaaf G, Ludewig U, Erenoglu BE, Mori S, Kitahara T, von Wirén N (2004)
ZmYS1 functions as a proton-coupled symporter for phytosiderophore- and nicotianamine-chelated metals.

J Biol Chem 279, 9091-9096

Review articles:

Bankaitis VA, Ile KE, Nile AH, Ren J, Ghosh R, Schaaf G (2012)

Thoughts on Sec14-like nanoreactors and phosphoinositide signaling.

Adv Biol Regul 52, 115-21

Bankaitis VA, Mousley CJ, Schaaf G (2010)

The Sec14 superfamily and mechanisms for crosstalk between lipid metabolism and lipid signaling.typo3/

Trends Biochem Sci 35, 150-60