Zentrum für Molekularbiologie der Pflanzen (ZMBP)




The light and electron microscopy service and research unit provides preparative techniques for morphological studies as well as for immunolocalisation of gene products. The focus of our methodological research is on cryoimmobilisation-based specimen preparation procedures (e.g., high pressure freezing and freeze-substitution) for improved preservation of structural integrity and antigenicity as well as on improving methods for detection of low copy number antigens. This methodological approach allows a detailed molecular analysis of cellular processes in the context of complex biological systems.

Techniques provided include negative staining, glycerol spraying, freeze-drying, cryoimmobilisation, freeze-substitution, microwave fixation and resin embedding, conventional resin embedding, low temperature embedding (methacrylates), ultrathin Tokuyasu cryosectioning & immunogold labelling, resin section immunogold labelling, pre-embedding immunogold labelling, critical-point drying, sputter coating, conventional and confocal light microscopy.

Scientists involved:

Dr. York-Dieter Stierhof Research Group Leader
Dr. Sandra Richter Senior Scientist

Technical Assistants:

Rebecca Stahl

Technician (80%)

Diana Vranjkovic Technician (50%)


Courses in Microscopy:

Elektronenmikroskopische Übungen
(6 Leistungspunkte):

“Anwendung der Elektronenmikroskopie in Zellbiologie, Mikrobiologie und Virologie”

Veranstalter: Dr. Katharina Hipp (Max-Planck-Institut für Entwicklungsbiologie), Dr. York-Dieter Stierhof (ZMBP), Dr. Sandra Richter (ZMBP), Dr. Petra Fallier-Becker (Institut für Pathologie, Universität Tübingen), Dr. Claus Burkhardt (NMI)
Termin: jedes WS; 26. Februar - 6. März 2020, ganztägiger 8-tägiger Ferienkurs
Vorbesprechung mit Platzvergabe: 11. Januar 2020, 9.00 Uhr, MPI für Entwicklungsbiologie, Max-Planck-Ring 5 (ehemals Spemannstrasse 35), Seminarraum Erdgeschoß
Weitere Auskünfte: Vorlesungskommentar
katharina.hipp(a)tuebingen.mpg.de (Tel: 07071-601310) oder york.stierhof(a)zmbp.uni-tuebingen.de (Tel: 07071-2976662)



Pflichtmodul: Methoden-Modul (Master, 3 Leistungspunkte)
im Rahmen des Master Programms in Biologie, "Cellular and Molecular Plant Biology" (Zelluläre und Molekulare Biologie der Pflanzen):

"Moderne Methoden in der Lichtmikroskopie"

Termin: nächster Kurs im SS 2020
Dr. Sandra Richter (ZMBP), Sven zur Oven-Krokhaus (ZMBP/Biophysikalische Chemie), Dr. Ulrike Mayer-Jürgens, Dr. York Stierhof (ZMBP), N.N.
Details werden noch bekanntgegeben
weitere Auskünfte: Dr. Sandra Richter
Tel: 29-76662
email: sandra.richter(a)zmbp.uni-tuebingen.de


Praktikum für Biochemiker zur Biologie und Biochemie der Pflanzen (Leiter Prof. G. Felix)

Teil: „Einführung in die Mikroskopie & Pflanzenstrukturen“

Inhalt: Bedienung des Lichtmikroskops, lichtmikroskopische Kontrastverfahren (Hellfeld, Dunkelfeld, Phasenkontrast, differenzieller Interferenzkontrast); Pflanzenstrukturen im Lichtmikroskop.
Termin: jedes SS, nach Ostern; 4 Gruppen
Dozenten: Dr. York-Dieter Stierhof, Dr. Sandra Richter
Weitere Auskünfte: york.stierhof(a)zmbp.uni-tuebingen.de


Praktikum Strukturaufklärung von Biomolekülen SAB (Leiter Prof. T. Stehle; Interfakultäres Institut für Biochemie IFIB):

Teil: „Abbildung von Molekülen und Molekülkomplexen in der Raster- und Transmissionselektronenmikroskopie“

Inhalt: Negativkontrastierung, Gefrierbruch, Gefriertrocknung, Schwermetall-Beschichtungstechniken, Glyzerin-Sprühtechnik, Cryofixierung, gefroren-hydratisierte Präparate, Cryo-TEM, (hochauflösende) Rasterelektronenmikroskopie.
Termin: SS und WS, jeweils 2 Vormittage (nächster Kurs: 29.+30. Januar 2020)
Ort: Auf der Morgenstelle 32, EM-Labor, Ebene 3
Dozenten: Dr. York-Dieter Stierhof, Dr. Sandra Richter
weitere Auskünfte und Anmeldung: Prof. Thilo Stehle (IFIB)


Konfokale und Epi-Fluoreszenzmikroskopie:
Einführung mit Übungen

Inhalt: Theorie und Praxis der Epi-Fluoreszenz- und konfokalen Lichtmikroskopie mit Übungen am Leica SP8 und/oder Zeiss LSM 880
Dauer: etwa 4 Stunden (Voraussetzung für selbständiges Arbeiten am Gerät)
Termin: nach Absprache
Ort: Auf der Morgenstelle 32, Mikroskopie, Ebene 3, Raum 3U01, 3R01 oder 3V01
weitere Auskünfte und Anmeldung: Dr. Sandra Richter sandra.richter(a)zmbp.uni-tuebingen.de   


Microscopy Course:

Inhalt: Lichtmikroskopie
Termin: Kurstermin wird noch festgelegt
Organisiert von Dr. Christian Feldhaus (MPI für Entwicklungsbiologie), in Zusammenarbeit mit Dr. York Stierhof (ZMBP), Dr. Sandra Richter (ZMBP), N.N.
Details werden noch bekanntgegeben
weitere Auskünfte: Dr. Christian Feldhaus (MPI für Entwicklungsbiologie)



„Immunmarkierung von Dünnschnitten für die Licht- und Elektronenmikroskopie“

Veranstalter: Dr. Heinz Schwarz und Dr. Katharina Hipp (Max-Planck-Institut für Entwicklungsbiologie) in Zusammenarbeit mit Dr. Bruno Humbel (Universität Utrecht) und Dr. York-Dieter Stierhof (ZMBP) mit Unterstützung der Deutschen Gesellschaft für Elektronenmikroskopie.
Termin: 30. September - 2. Oktober 2020
Weitere Auskünfte: Katharina Hipp (katharina(a)tuebingen.mpg.de) oder York Stierhof (york.stierhof(a)zmbp.uni-tuebingen.de)





Recent Publications (Electron microscopy):

1998 1999 2000 2001 2002 2003 2004 2005 2006 2007 2008 2009 2010 2011 2012 2013 2014 2015


Overath, P., Ruoff, J., Stierhof, Y.-D., Haag, J., Tichy, H., Dykova, I. and J. Lom (1998).
Cultivation of bloodstream forms of Trypanosoma carassii, a common parasite of freshwater fishes.
Parasitol. Res., 84, 343-347.

Fry, A.M., Mayor, T., Meraldi, P., Stierhof, Y.-D., Tanaka, K. and A.E. Nigg (1998).
C-Nap1, a novel centrosomal coiled-coil protein and candidate substrate of the cell cycle-regulated protein kinase Nek2.
J. Cell Biology, 141, 1563-1574.

Pohl, K. and Y.-D. Stierhof (1998).
Action of gold chloride („gold toning“) on silver enhanced 1 nm gold markers.
Microsc. Res. Techn., 42, 59-65.

Baschong, W. and Y.-D. Stierhof (1998). Preparation, use, and enlargement of ultrasmall gold particles in immunoelectron microscopy.
Microsc. Res. Techn., 42, 66-79.

Stierhof, Y.-D., Wiese, M., Ilg, T., Overath, P., Häner, M. and U. Aebi (1998).
Structure of a filamentous phosphoglycoprotein polymer: the secreted acid phosphatase of Leishmania mexicana.
J. Molecular Biology, 282, 137-148.


Scory, S., Caffrey, C.R., Stierhof, Y.-D., Ruppel, A. and D. Steverding (1999).
Trypanosoma brucei: killing of bloodstream forms in vitro and in vivo by the cystein proteinase Z-Phe-Ala-CHN2.
Exp. Parasitol., 91, 327-333.

Maeting, I., Schmidt, G., Sahm, H., Revuelta, J.L., Stierhof, Y.-D- and K.-P. Stahmann (1999).
Isocytrate lyase of Ashbya gossypii – Transcriptional regulation and peroxisomal localization.
FEBS Lett., 444, 15-21.

Ilg, T., Handman, E., Ng, K., Stierhof, Y.-D and A. Bacic (1999).
Mucin-like proteophosphoglycans from the protozoan parasite Leishmania.
Trends in Glycoscience and Glycotechnology, 11, 53-71.

Ilg, I. , Handman, E. and Y.-D. Stierhof (1999).
Proteophosphoglycans from Leishmania promastigotes and amastigotes.
Biochem. Soc. Transactions, 27, 518-525.

Mayor, T., Meraldi, P., Stierhof, Y.-D., Nigg, E.A. and A.M. Fry (1999).
Protein kinases in control of the centrosome cycle.
FEBS Letters, 452, 92-95.

Ilg, T., Montgomery , J., Stierhof, Y.-D. and E. Handman (1999).
Molecular cloning and characterization of a novel repeat-containing L. major gene, ppg1, that encodes a membrane assocoated form of proteophosphoglycan with a putative glycosylphosphatidylinositol anchor.
J. Biol. Chemistry, 274, 31410-31420.

Stierhof, Y.-D., Bates, P.A., Jacobson, R.L., Rogers , M.E., Schlein, Y., Handman, E. and T. Ilg (1999).
Filamentous proteophosphoglycan secreted by Leishmania promastigotes forms gel-like three-dimensional networks that obstruct the digestive tract of infected sandfly vectors.
Eur. J. Cell Biology, 78, 675-689.

Klein, C., Goepfert, U., Goehring, N., Stierhof, Y.-D. and T. Ilg (1999).
Proteophosphoglycans of Leishmania mexicana: Identification, purification, structural and ultrastructural characterization of the secreted promastigote proteophosphoglycan pPPG2, a stage-specific glycoisoform of amastigote aPPG.
Biochem. J., 344, 775-786.


Lischke, A., Klein, C., Stierhof, Y.-D,, Hempel, M., Mehlert A., De Almeida, I.C., Ferguson, M.A.J. and P. Overath (2000).
Isolation and characterization of GPI-anchored, mucin-like surface glycoproteins from bloodstream forms of the freshwater fish parasite. Trypanosoma carassii.
Biochem. J., 345, 693-700.

Mayor, T., Stierhof, Y.-D., Tanaka, K., Fry, A. M. and E.A. Nigg (2000).
The centrosomal protein C-Nap1 is required for cell cycle-regulated centrosome cohesion.
J. Cell Biology, 151, 837-846.

Weise, F., Stierhof, Y.-D., Kühn, C., Wiese, M. and P. Overath (2000).
Distribution of GPI-anchored proteins in the protozoan parasite Leishmania based on an improved ultrastructural description using high-pressure-frozen cells.
J. Cell Science, 113, 4587-4503.


Knüpfer, K,, Stierhof, Y.-D., McKean, P.G. and D.F. Smith (2001).
Characterisation of a differentially-expressed protein that shows an unusual localisation to intracellular membranes in Leishmania major.
Biochemical J., 356, 335-444.

Völker, A., Stierhof, Y.-D. and G. Jürgens (2001).
Cell cycle-independent expression of the Arabidopsis cytokinesis specific synthaxin KNOLLE results in mistargeting to the plasma membrane and is not sufficient for cytokinesis.
J. Cell Science, 114, 3001-3012.

Caffrey, C.R., Hansell, E., Lucas, K.D., Brinen, L.S., Hernandez, A.A., Cheng, J., Gwaltney II, S.L., Roush, W.R., Stierhof, Y.-D., Bogyo, M., Steverding, D. and J.H. McKerrow (2001).
Active site mapping, biochemical properties and subcellular localization of rhodesain, the major cysteine protease of Trypanosoma brucei rhodesiense.
Mol. Biochem. Parasitol., 118, 61-73.

Legare, D., Richard, D., Mukhopadhyay, M., Stierhof, Y.-D., Rosen, B.P., Haimeur, A., Paoadopoulou, B. and M. Ouellette (2001).
The Leishmania ABC transporter PGPA is an intracellular metal-thiol ATPase.
J. Biol. Chemistry, 276, 26301-26307.

Geldner, N., Friml, J., Stierhof, Y.-D., Palme, K. and G. Jürgens (2001).
Auxin-transport inhibitors block efflux carrier PIN1 cycling and vesicle trafficking.
Nature, 413, 425-428.


Schnittger, A., Schöbinger, U., Bouyer, D., Weinl, C., Stierhof, Y.-D. and M. Hülskamp (2002).
Ectopic D-type cyclin expression induces not only DNA replication but also cell division in Arabodpsis trichomes.
Proc. Natl. Acad. Sci. U.S.A., 99, 6410-6415.

Steinborn, K., Maulbetsch, C., Priester, B., Trautmann, S., Pacher, T., Geiges, B., Küttner, F., Lepiniec, L., Stierhof, Y.-D., Schwarz, H. and U. Mayer (2002).
The Arabidopsis PILZ group encode tubulin-folding cofactor orthologs required for cell division but not cell growth.
Genes Dev., 16, 959-971.

Schnittger, A., Schöbinger, U., Stierhof, Y.-D. and M. Hülskamp (2002).
Ectopic b-type cyclin expression is sufficient to switch from endoreduplication to mitotic cycles in Arabidopsis trichomes.
Current Biology, 12, 451-420.

Grünfelder, C., Engstler, M., Weise, F., Schwarz, H., Stierhof, Y.-D., Boshart, M. and P. Overath (2002).
Accumulation of a GPI-achored protein at the cell surface requires sorting at multiple intracellular levels.
Traffic, 3, 547-559.

Mayor, T., Hacker, U., Stierhof, Y.-D. and E.A. Nigg (2002).
The mechanism regulating the dissociation of the centrosomal protein C-Nap1 from mitotic spindle poles.
J. Cell Science, 15, 3275-3284.

Vötsch, W., Nicholson, G., Müller, R., Stierhof, Y.-D., Gorb, S. and U. Schwarz (2002).
Chemical composition of the attachment pad secretion of the locust Locus schistocerca gregaria.
Insect Biochem. Molec. Biol., 32, 1605-1613.


Grünfelder, C. G., Engstler, M., Weise, F., Schwarz, H., Stierhof, Y.-D., Morgan, G.W., Field, M.C. and P. Overath (2003).
Endocytosis of a GPI-anchored protein via clathrin-coated vesicles, sorting by default in endosomes and exocytosis via rab11-positive carriers.
Molec. Biol. Cell, 14, 2029-2040.


Schrick, K., Fujioka, S., Takatsuto, S., Stierhof, Y.-D., Stranski, H., Yoshida, S. and G. Jürgens (2004).
A link between sterol biosynthesis, the cell wall and cellulose in Arabidopsis.
Plant Journal, 38, 227-243. (Erratum: 2004, 38, 562)

Okumoto, S., Koch, W., Tegeder, M., Fischer, W. N., Stierhof, Y.-D., and W. B. Frommer (2004).
Root phloem-specific expression of the plasma membrane amino acid proton co-transporter AAP3.
J. Experimental Botany, 55, 2155-2168.

Wei-Chih Su, M., Pyarajan, S., Chang, J.-H., Yu, C.-L., Jin, Y.-J., Stierhof, Y.-D., P. Walden, and S. A. Burakoff (2004).
Fratricide of CD8+ cytotoxic T lymphocytes is dependent on cellular activation and perforin-mediated killing.
Eur. J. Immunology, 34, 2459-2470.


Sampaio, K.L., Cavignac, Y., Stierhof, Y.-D., and C. Sinzger (2004).
Human cytomegalovirus with green fluorescent protein for live analysis of intracellular particle movements.
J. Virology, 79, 2754-2767.


Strompen, G., Dettmer, J., Stierhof, Y.-D., Schumacher, K., Jürgens, G. and U. Mayer (2004).
The Arabidopsis TUFF gene encodes isoform 1 of vacuolar H+-ATPase subunit E and is required for embryogenesis.
Plant Journal, 41, 125-132.

Dettmer, J., Schubert, D., Calvo-Weimar, O., Stierhof, Y.-D., Schmidt, R. and K. Schumacher (2004).
V-ATPase is essential for Golgi organisation in higher plants.
Plant Journal, 41, 117-124.

Guarguaglini, G., Duncan, P.I., Stierhof, Y.-D., Holmström, T., Duensing, S. and E.A. Nigg (2005).
The forkhead-associated domain protein Cep170 interacts with Plk1 and serves as a marker for mature centrioles.
Molec. Biol. Cell, 16, 1095-1107. (Epub: 2004, Dec 22)

Paciorek, T., Zazimalova, E., Ruthardt, N., Petrasek, J., Stierhof, Y.-D., Kleine-Vehn, J., Morris, D.A., Emans, N., Jürgens, G. and J. Friml (2005).
Auxin inhibits endocytosis and promotes its own efflux from cells.
Nature, 435, 1251-1256.

Habedanck, R., Stierhof, Y.-D., Wilkinson, C.J. and E.A. Nigg (2005).
The Polo kinase Sak/Plk4 regulates centriole duplication.
Nature Cell Biology, 7, 1140-1146. (Epub Oct 23).

Bahe, S., Stierhof, Y.-D., Wilkinson, C. J.; Leiss, F. and E.A. Nigg (2005).
Rootletin forms centriole-associated filaments and functions in centrosome cohesion.
J. Cell Biology, 171, 27-33.


Drumea-Mirancea, M., Wessels, J. T., Müller, C. A., Essl, M., Eble, J. A., Tolosa, E., Koch, M., Reinhardt, D. P., Sixt, M., Sorokin, L., Stierhof, Y.-D., Schwarz, H. and G. Klein (2006).
Characterization of a laminin-5 containing conduit system in the human thymus – a transport system for small molecules.
J. Cell Science, 119, 1396-1405.

Dettmer, J., Hong-Hermesdorf, A., Stierhof, Y.-D. and K. Schumacher (2006).
Convergence of the early endocytic and secretory pathways in the trans-Golgi network of Arabidopsis.
Plant Cell, 13, 715-730.


Van der Fecht-Bartenbach, J., Bogner, M., Krebs, M., Stierhof, Y.-D., Schumacher, K. and U. Ludewig (2007).
Function of the anion transporter AtCLC-d in the trans-Golgi-network.
Plant Journal, 50, 466-474.

Scory, S., Stierhof, Y.-D., Caffrey, C.R., and D. Steverding (2007).
The cystein proteinase inhibitor Z-Phe-Ala-CHN2 alters the cell morphology and cell division activity of Trypanosoma brucei bloodstream forms in vivo.
Kinetoplastid Biology and Disease, 6:2, 28 February, doi:10.1186/1475-9292-6-2

Proikas-Cezanne, T., Ruckerbauer, S., Stierhof, Y.-D., Berg, C. and A: Nordheim (2007).
Human WIPI-1 puncta-formation: A novel assay opportunity for mammalian autophagy.
FEBS Letters, 581, 3396-3404.

Dhonukshe, P., Aniento, F., Hwang, I., Robinson, D.G., Mravec, J., Stierhof, Y.-D., and J. Friml (2007).
Clathrin-mediated constitutive endocytosis of PIN Auxin efflux carriers in Arabidopsis.
Current Biology, 17, 1-8.

Richter, S., Geldner, N., Schrader, J., Stierhof, Y.-D., Rios, G., Koncz, C., Robinson, D.G. and G. Jürgens (2007).
Functional diversification of closely related ARF-GEFs in protein secretion and recycling.
Nature 448, 488-492.

Kleine-Sohn, J., Westendorf, J., Celleck, M., Stierhof, Y.-D. and E. Nigg (2007).
PLK-induced centriole biogenesis in human cells.
Developmental Cell 13, 190-202.

Graser, S., Stierhof, Y.-D., Gassner, O.S., Lavoie, S.B., Lamla, S., Le Clech, M. and E.A. Nigg (2007).
Cep164, a novel centriole appendage protein required for primary cilium formation.
J. Cell Biology 179, 321-330.

Graser, S., Stierhof, Y.-D. and E.A. Nigg (2007).
Cep68 and Cep215 (Cdk5rap2) are required for centrosome cohesion.
J. Cell Science 120, 4321-4331.

Reichardt, I., Stierhof, Y.-D., Mayer, U., Richter, S., Schwarz, H., Schumacher, K. and G. Jürgens (2007).
Plant cytokinesis requires de novo secretory trafficking but not endocytosis.
Current Biology 17, 1-7.


Humbel, B. M. and Y.-D. Stierhof (2008).
Cryo-sectioning according to Tokuyasu (Chapter 19).
In: Handbook of Cryo-Preparation Methods for Electron Microscopy. Cavalier, A., Spehner, D., Humbel, B.M. eds.,
CRC Press Boca Raton, pp 467-498.

Stierhof, Y.-D. (2008).
Immunolabelling of ultrathin sections with enlarged 1 nm gold or Qdots (Chapter 23).
In: Handbook of Cryo-Preparation Methods for Electron Microscopy. Cavalier, A., Spehner, D., Humbel, B.M. eds.,
CRC Press, Boca Raton, pp. 587-616.

Stierhof, Y.-D., van Donselaar, E., Schwarz, H., and B.M. Humbel (2008).
Cryo-fixation, freeze-substitution, rehydration and Tokuyasu cryo-sectioning (Chapter 14).
In: Handbook of Cryo-Preparation Methods for Electron Microscopy. Cavalier, A., Spehner, D., Humbel, B.M. eds.,
CRC Press, Boca Raton, pp. 343-366.

Ripper, D., Schwarz, H., and Y.-D. Stierhof (2008).
Cryo-section immunolabelling of difficult to fix specimens: Advantages of cryofixation, freeze-substitution and rehydration.
Biology of the Cell, 100, 109-123.
Epub 2007 Sept 28, DOI:10.1042/BC20070106

Men, S., Boutté, Y., Ikeda, Y., Li, X., Palme, K., Stierhof, Y.-D., Hartmann, M.-A., Moritz, T. and M. Grebe (2008).
Sterol-dependent endocytosis mediates post-cytokinetic acquisition of PIN2 auxin efflux carrier polarity.
Nature Cell Biology, 10, 237-244.

Horak, J., Grefen, C., Berendzen, K.W., Hahn, A,. Stierhof, Y.-D., Stadelhofer, B., Stahl, M., Koncz, C. and K. Harter (2008).
The Arabidosis thaliana response regulator ARR22 is a putative AHP phospho-histidine phosphatase expressed in the chalaza of developing seeds.
BMC Plant Biology, 8, 77.

Jiang, X. J., Adler, B., Laib Sampaio, K., Digel, M., Jahn, G., Ettischer, N., Stierhof, Y.-D., Scrivano, L., Koszinowski, U., Mach, M. and C. Sinzger (2008).
UL74 of human cytomegalovirus contributes to release of virions and to cell-associated spread in fibroblasts.
J. Virology, 82, 2802-2812.

Anders, N., Nielsen, M., Keicher, K., Stierhof, Y.-D., Furutani, M., Tasaka, M., Skriver, K. and G. Jürgens (2008).
Membrane association of the Arabidopsis ARF exchange factor GNOM involves interactions of conserved domains.
Plant Cell, 20, 142-151.

Dhonukshe, P., Grigoriev, I., Fischer, R., Tominaga, M., Robinson, D.G., Hasek, J., Paciorek, T., Petrasek, J., Seifertova, D., Teios, R., Meisel, L.A., Zazimalova, E., Gadella Jr, T.W.J., Stierhof, Y.-D., Ueda, T., Oiwa, K., Akhmanova, A., Brock, R., Spang, A. and J. Friml (2008).
Auxin transport inhibitors impair vesicle motility and actin cytoskeleton dynamics in diverse eukaryotes.
Proc. Natl. Acad. Sci. USA, 105, 4489-4494.

Brüx, A., Liu, T.-Y., Krebs, M., Stierhof, Y.-D., Lohmann, J. U., Miersch, O., Wasternack, C. and K. Schumacher (2008).
Reduced V-ATPase activity in the trans-Golgi network causes oxylipin-dependent hypocotyl growth inhibition in Arabidopsis.
Plant Cell, 20, 1088-1100.

Neubert, C., Graham, L.A., Black-Maier, E.W., Coonrod, E.M., Liu, T.-J., Stierhof, Y.-D., Seidel, T., Stevens, T.H. and K. Schumacher (2008).
Arabidopsis has two functional orthologs of the yeast V-ATPase assembly factor Vma21p.
Traffic, 9, 1618-1628.


Elgass, K., Caesar, K., Stierhof, Y.-D., Meixner A. J. and K. Harter (2009).
Wavelength-selective and fluorescence lifetime laser microscopy reveals novel dynamics in living plant cells
PLoS ONE, Epub 2009 May 27, e5716.

Krupnova, T., Sasabe, M., Ghebreghiorghis, L., Gruber, Ch.W., Hamada, T., Dehmel, V., Strompen, G., Stierhof, Y.-D., Lukowitz, W., Kemmerling, B., Machida, Y., Hashimoto, T., Mayer, U. and G. Jürgens (2009).
Micreotubule-associated kinase-like protein RUNKEL is required for cell plate expansion in Arabidopsis cytokinesis.
Current Biology, 19, 518-523.

Crowell, E.F., Bischoff, V., Desprez, T., Rolland, A., Stierhof, Y.-D., Schumacher, K., Gonneau, M., Höfte H. and S. Vernhettes (2009).
Pausing of Golgi bodies on microtubules regulates secretion of cellulose synthase complexes in Arabidopsis.
Plant Cell, 21, 1141-1154.

Geldner, N., Denervaud-Tendon, V., Hyman, D. L., Mayer, U., Stierhof, Y.-D. and J. Chory (2009).
Rapid, combinatorial analysis of membrane compartments in intact plants with a muti-color marker set.
Plant Journal, 59, 169-178.

Schmidt, T.I., Kleylein-Sohn, J., Westendorf, J., Le Clech, M., Lavoie, S.B., Stierhof, Y.-D. and E.A. Nigg (2009).
Control of centriole length by CPAP and CP110.
Current Biology, 19, 1005-1011.

Mravec, J., Skupa, P., Bailly, A., Krecek, P., Hoyerova, K., Bielach, A., Petrasek, J., Zhang, J., Gaykova, V., Stierhof, Y.-D., Schwarzerove, K., Rolcik, J, Dobrev, P.I., Seifertova, D., Luschnig, C., Benkova, E., Zazimalova, E., Geisler, M. and J. Friml (2009).
Subcellular homeostasis of phytohormone auxin is mediated by the ER-localized PIN5 transporter.
Nature, 459, 1136-1140.

Lee, S., Lee, D.W., Lee, Y., Mayer, U., Stierhof, Y.-D., Lee, S., Jürgens, G. and I. Hwang (2009).
Heat shock protein cognate 70-4 and an E3 ubiquitin ligase, CHIP, mediate plastid-destined precursor degradation through the ubiquitin-26S proteasome system in Arabidopsis.
Plant Cell, 21, 3984-4001.


Stierhof, Y.-D. and F. El-Kasmi (2010).
Strategies to improve the antigenicity, ultrastructure preservation and visibility of trafficking compartments in Arabidopsis tissue.
Eur. J. Cell Biology 89, 285-297.

Yang, H., Bogner, M., Stierhof, Y.-D. and U. Ludewig (2010).
H-independent glutamine transport in plant root tips.
PLoS One 5, Epub Jan 27, e8917.

Krebs, M., Beyl, D., Görlich, E., Al-Rasheid, K.A., Marten, I., Stierhof, Y.-D., Hedrich, R. and K. Schumacher (2010).
Arabidopsis V-ATPase activity at the tonoplast is required for efficient nutrient storage but not for sodium accumulation.
Proc. Natl. Acad. Sci. USA 107, 3251-3256.

Simon, U.K., Groenewald, J.Z., Stierhof, Y.-D., Crous, P.W. and R. Bauer (2010).
Mycosphaerella podagraria – a necrotrophic phytopathogen forming a special cellular interaction with its host Aegopodium podeagraria.
Mycological Progress, 9, 49-56.

Tschan, S., Kreidenweiss, A., Stierhof, Y.-D,, Sessler, N., Fendel, R., and B. Mordmüller (2010).
Mitochondrial localization of the threonine peptidase PfHslV, a ClpQ ortholog in Plasmidium falciparum.
International Jounal of Parasitology, 40, 1517-1523.

Viotti, C., Bubeck, J., Stierhof, Y.-D., Krebs, M., Langhans, M., van den Berg, W., von Dongen, W., Richter, S., Geldner, N., Takano, J., Jürgens, G., de Vries, S.C., Robinson, D.G., and K. Schumacher (2010).Endocytic and secretory traffic in Arabidopsis merge in the trans-Golgi network/early endosome, an independent and highly dynamic organelle.
Plant Cell, 22, 1344-1357.

Isono, E., Katsiarimpa, A., Müller, I.K., Anzenberger, F., Stierhof, Y.-D., Geldner, N., Chory, J., and C. Schwechheimer (2010).
The deubiquitinating enzyme AMSH3 is required for intracellular trafficking and vacuole biogenesis in Arabidopsis thaliana.
Plant Cell, 22, 1826-1837.

Crowell, E.F., Gonneau, M., Stierhof, Y.-D., Höfte, H., and S. Vernhettes (2010).
Regulated trafficking of cellulose synthases.
Current Opinion in Plant Biology, 13, 700-705.

Kaegi, C., Baumann, N., Nielsen, N., Stierhof, Y.-D., and R. Groß-Hardt (2010).
The gametic central cell of Arabidopsis determines the lifespan of adjacent accessory cells.
Proceedings National Academic Sciences U.S.A., 107, 22350-22355.


Mauthe, M., Jacob, A., Freiberger, S., Hentschel, K., Stierhof, Y.-D., Codogno, P., and T. Proikas-Cezanne (2011).
Resveratrol-mediated autophagy requires WIPI-1 regulated LC3 liquidation in the absence of induced phagophore formation.
Autophagy, 7, 1448-1461.

Vogelmann, J., Ammelburg, M., Finger, C., Guezguez, J., Linke, D., Flötenmeyer, M., Stierhof, Y.-D., Wohlleben, W., and G. Muth (2011).
Conjugal plasmid transfer in Streptomyces resembles bacterial chromosome segregation by FtsK/SpoIIIE.
EMBO Journal, 30, 2246-2254.

Wagner, B.J., Lindau, D., Ripper, D., Stierhof, Y.-D., Glatzle, J., Witte, M., Beck, H., Keppeler, H., Lauber, K., Rammensee, H.-G., and A. Königsrainer (2011).
Phagocytosis of dying tumor cells by human peritoneal mesothelial cells.
J. Cell Science, 124, 1644-1654.

Jiang, X.J., Laib Sampaio, K., Ettischer, N., Stierhof, Y.-D., Jahn, G., Kropf, B., Mach, M., and C. Sinzger (2011).
UL74 of human cytomegalovirus reduces the imhibitory effect of gH-specific and gB-specific antibodies.
Archives of Virology, 156, 2145-2155.

Vaten, A., Dettmer, J., Wu, S., Stierhof, Y.-D., Roberts, C.J., Yadev, S.R., Miyashima, S., Campilho, A., Bulone, V., Lichtenberger, R., Lehesranta, S., Mähönen, A.P., Kim, J., Sauer, N., Scheres, B., Carlsbecker, A., Gallagher, K.L., and Y. Helariutta (2011).
Callose biosynthesis regulates symplastic trafficking during root development.
Developmental Cell, 21, 1144-1155.

El-Kasmi, F., Pacher, T., Strompen, G., Stierhof, Y.-D., Müller, L., Koncz, C., Mayer, U., and G. Jürgens (2011).
Arabidopsis SNARE protein SEC22 is essential for gametophyte development and maintenance of Golgi-stack integrity.
Plant Journal, 66, 268-279.

Roppolo, D., de Rybel, B., Denervaud-Tendon, V., Pfister, A., Alassimone, J., Vermeer, J.E.M., Yamazaki, M., Stierhof, Y.-D., Beeckman, T., and N. Geldner (2011).
A novel protein family mediates Casparian strip formation in the endodermis.
Nature, 473, 380-383.

Djamei, A., Schipper, K., Rabe, F., Vincon, V., Kahnt, J., Osorio, S., Fernie, A.R., Johannsen, M., Maier, U., Mörschel, E., Feussner, I., Feussner. F., Meinicke, P., Stierhof, Y.-D., Schwarz, H., Maček, B., Mann, M., and R. Kahmann (2011).
Metabolic priming by a secreted fungal effector.
Nature, 478, 395-398.

Wagner, C., Polke, M., Gerlach, R.G., Linke, D., Stierhof, Y.-D., Schwarz, H., and M. Hensel (2011).
Functional dissection of SiiE, a giant non-fimbrial adhesin of Salmonella enterica.
Cellular Microbiology, 13, 1286-1301.

Touihri, S., Knöll, C., Stierhof, Y.-D., Müller, I., Mayer, U., and G. Jürgens (2011).
Functional anatomy of Arabidopsis cytokinesis-specific syntaxin KNOLLE.
Plant Journal, 68, 755-764.


Richter, S., Müller, L.M., Stierhof, Y.-D., Mayer, U., Takada, N., Kost, B., Vieten, A., Geldner, N., Koncz, C., and G. Jürgens (2012).
Polarised cell growth in Arabidopsis requires endosomal recycling mediated by GBF1-related ARF exchange factors.
Nature Cell Biology, 14, 80-86.

Arquint, C., Sonnen, K.F., Stierhof, Y.-D., and E.A. Nigg (2012).
Cell cycle-regulated expression of STIL controls centriole numbers in human cells.
J. Cell Science, 125, 1342-1352

Haspot, F., Lavault, A., Sinzger, C., Laib Sampaio, K., Stierhof, Y.-D., Pilet, P., Bressolette-Bodin, C., and F.A. Halary (2012).
Human cytomegalovirus entry into dentritic cells occurs via a macropinocytosis-like pathway in a pH-independent and cholesterol-dependent manner.
PLoS One, 7(4), e34795, Epub 2012 Apr 9.

Robinson, D.G., Pimpl, P., Scheuring, D., Stierhof, Y.-D., Sturm, S., and C. Viotti (2012).
Trying to make sense of retromer.
Trends in Plant Science, 17, 431-439.

Pfannmöller, M., Röder, I.V., Benner, G., Stierhof, Y.-D., Wacker, I.U., and R.R. Schröder (2012).
Detecting correlative light and electron microscopic labels by electron spectroscopic imaging.
Biomedical Imaging (ISBI) (2012 9th IEEE International Symposium on Biomedical Imaging: From Nano to Macro) 2-5 May 2012, Spain

Ding, Y., Wang, J., Wang, J., Stierhof, Y.-D., Robinson, D.G., and L. Jiang (2012).
Unconventional protein secretion.
Trends in Plant Science, 17, 606-615.

Brandt, R., Xie, Y., Musielak, T., Graeff, M., Stierhof, Y.-D., Huang, H., Liu, C.-M., and S. Wenkel (2012).
Control of stem cell homeostasis via interlocking microRNA and microProtein feedback loops.
Mechanisms of Development, July 7 (Epub ahead of print), (2013) 130, 25-33.

Stierhof, Y.-D., Viotti, C., Scheuring, D., Sturm, S., and D.G. Robinson (2012).
Sorting nexins 1 and 2a locate mainly to the TGN.
Protoplasma, March 24 (Epub ahead of print), (2013) 250, 235-240.


Krupnova, T. , Stierhof, Y.-D., Hiller, U., Strompen, G., and S. Müller (2013).
Microtubule associated kinase-like protein RUNKEL functions in somatic and non-conventional cytokinesis.
The Plant Journal, 74, 781-791.

Park, M., Song, K,, Reichert, I., Kim, H., Mayer, U., Stierhof, Y.-D., Hwang, I., and G. Jürgens (2013).
Arabidopsis µ-adaptin subunit AP1M of adaptor complex 1 mediates late secretory and vacuolar traffic and is required for growth.
Proceedings National Academic Sciences U.S.A., 110, 10318-10323.

Griessl, M.H., Schmid, B., Kassler, K., Braunsmann, C., Ritter, R., Barlag, B., Stierhof, Y.-D., Sturm, K.U., Danzer, C., Wagner, W., Schäffer, T.E., Sticht, H., Hensel, M., and Y.A. Muller (2013).
Structural insight into the giant Ca2+-binding adhesin SiiE: implications for the adhesion of Samonella enterica to polarized epithelial cells.
Structure, 21, 741-752.

Sonnen, K.F., Gabryjonczyk, A.-M., Anselm, E., Stierhof, Y.-D., and E.A. Nigg (2013).
Human Cep192 and Cep152 cooperate in PLK4 recruitment and centriole duplication.
J. Cell Science, 126, 3223-3233.

Sauer, M., Lopez*, M.O.D., Zouhar*, J., Reynolds, G., Liljegren, S., Stierhof, Y.-D., de Jaeger, G., Otegui, M., Bednarek, S., and E. Rojo (2013).
MTV1 and MTV4 encode plant specific ENTH and ARF GAP proteins that mediate chlathrin-dependent trafficking of vacuolar cargo from the trans-Golgi Network.
Plant Cell, 25, 2217-2235.

El-Kasmi, F., Krause, C., Hiller, U., Stierhof, Y.-D., Mayer, U., Conner, L., Kong, L., Reichardt, I., Sanderfoot, A.A., and G. Jürgens (2013).
SNARE complexes of different composition jointly mediate membrane fusion in Arabidopsis cytokinesis.
Molecular Biology of the Cell, 21, 1593-1601.



Nicole Klüglein, Fabian Zeitvogel, York-Dieter Stierhof, Matthias Flötenmeyer, Kurt O. Konhauser, Andreas Kappler, and Martin Obst (2014).
Potential role of nitrite for abiotic Fe(II) oxidation and cell encrustation during nitrate reduction by denitrifying bacteria.
Applied and Environmental Microbiology, 80, 1051-1061.

Gregor Schmid, Fabian Zeitvogel, Likai Hao, Pablo Ingenio, Matthias Flötenmeyer, York-Dieter Stierhof, Birgit Schroeppel, Claus Burkhardt, Andreas Kappler, and Martin Obst (2014).
3D analysis of bacterial cell-(iron)mineral aggregates formed by the nitrate-reducing, Fe(II)-oxidizing Acidovorax sp. Strain BoFeN1 using complementary microscopy tomograhphy approaches.
Geobiology, 12, 340-361.

Manoj Singh, Falco Krüger, Hauke Beckmann, Sabine Brumm, Joop Vermeer, Teun Munnik, Ulrike Mayer, York-Dieter Stierhof, Christopher Grefen, Karin Schumacher, and Gerd Jürgens (2014).
Arabidopsis SAND protein-dependent Rab GTPase conversion is dispensible for endosomal maturation but essential for MVB-vacuole fusion.
Current Biology, 24, 1383-1389.

Sandra Richter, Marika Kientz, Sabine Brumm, Mads Eggert Nielsen, Misoon Park, Richard Gavidia, Cornelia Krause, Ute Voss, Hauke Beckmann, Ulrike Mayer, York-Dieter Stierhof, Gerd Jürgens (2014).
Delivery of endocytosed proteins to the cell–division plane requires change of pathway from recycling to secretion.
eLife 2014, 3:e02131.

Huaiyu Yang, Melanie Krebs, York-Dieter Stierhof, and Uwe Ludewig (2014).
Characterization of the putative amino acid transporter genes AtCAT2, 3 and 4: the tonoplast localized AtCAT2 regulates soluble leaf amino acids.
J. Plant Physiology, 171, 594-601.

Jan Dettmer, Robertas Ursache, Ana Campiljo, Shunsuke Miyashima, Ilya Belevich, Seana O´Regan, Daniel Leroy Mullendore, Shri Ram Yadav, Christa Lanz, Luca Beverina, Antonio Papagni, Korbinian Schneeberger, Detlef Weigel, York-Dieter Stierhof, Thomas Moritz, Michael Knoblauch, Eija Jokitalo and Ykä Helariutta (2014).
CHOLINE TRANSPORTER LIKE1 (CHER1) is required for sieve plate development to mediate long distance cell-to-cell communication.
Nature Communications, 5, 4276.


David J. Sheerin, Chiara Menon, Sven zur Oven-Krockhaus, Philipp Johnen, Frank Schleifenbaum, York-Dieter Stierhof, and Andreas Hiltbrunner (2015).
light-activated phytochrome A and B interact with members of the SPA1 family to promote photomorphogenesis in Arabidopsis by disrupting the COP1-SPA complex.
Plant Cell, 27, 189-201.

Huaiyu Yang, York-Dieter Stierhof, and Uwe Ludewig (2015).
The putative cationic Amino acid transporter 9 is targeted to vesicles and may be involved in plant amino acid homeostasis.
Frontiers in Plant Science, 2015 Apr 1; 6:212. eCollection 2015.

Yu Luo, Stefan Scholl, Anett Doering, Yi Zhang, Niloufer Irani, Simone Di Rubbo, Lutz Neumetzler, Praveen Krishnamoorthy, Isabelle Van Houtte, Evelien Mylle, Volker Bischoff, Samantha Vernhettes, Johan Winne, Jiri Friml, York-Dieter Stierhof, Karin Schumacher, Staffan Persson, and Eugenia Russinova (2015).

V-ATPase activity in the TGN/EE is required for exocytosis and recycling in Arabidopsis.
Nature Plants, 1, 1-10; 6 Juli 2015, article number: 15094,
DOI: 10.1038/NPLANTS.2015.94

Julia V. Monjarás Feria, Matthew D. Lefebre, York-Dieter Stierhof, Jorge E. Galán, and Samuel Wagner (2015).

Role of autocleavage in the function of a type III secretion specificity switch protein in Salmonella typhimurium.

mBio, 2015 Oct13; 6 (5). Pii: e01459-15. DOI: 10.1128/mBio.01459-15.

Charlotte Kirchhelle, Cheung-Ming Chow, Camille Foucart, Helia Neto, York-Dieter Stierhof, Monika Kalde, Carol Walton, Mark Fricker, Richard S. Smith, Antoine Jerusalem, Niloufer Irani, and Ian Moore (2016).
The specification of geometric edges by a plant Rab GTPase ia an essential cell-patterning principle during organogenesis in Arabidopsis.
Developmental Cell, 36, 386-400.


Susann Zilkenet, Mirita Franz-Wachtel, York-Dieter Stierhof, Jorge E. Galan, Boris Macek, Samuel Wagner (2016).
Determination of the stoichiometry of the complete bacterial type III secretion needle complex using a combined quantitative proteomic approach.
Molecular and Cellular Proteomics, 15, 1598-1609. (Epub 2016 Feb 21)).

Xehua Li, Cengiz Koc, Petra Kühner, York-Dieter Stierhof, Bernhard Krismer, Mark Enright, Jose Penades, Christiane Wolz, Thilo Stehle, Christian Cambillau, Andreas Peschel, and Guoqing Xia (2016).
An essential role for the baseplate protein Gp45 in phage adsorption to Staphylococcus aureus.
Scientific Reports, May 23, 6:26455


David G. Tekleyohans, Yanbo Mao, Christina Kägi, York-Dieter Stierhof, and Rita Gross-Hardt (2017).
Polyspermy – a plant perspective.
Current Opinion in Plant Biology, 35, 131-137.

Shuping Xing, Dietmar Mehlhorn, Niklas Wallmeroth, Lisa Jasmin Assek, Ritwika Kar, Alessa Voß, Philipp Denninger, Vanessa Aphaia Fiona Schmidt, Markus Schwarzländer, York-Dieter Stierhof, Guido Grossmann, and Christopher Grefen (2017).
Loss of GET pathway orthologues in Arabidopsis thaliana causes root hair growth defects and affects SNARE abundance.
Proceedings National Academic Sciences U.S.A., 114, E1544-E1553. (Epub 2017 Jan 17)


Park, M., Krause, C., Karnahl, M., Reichardt, I., El Kasmi, F., Mayer, U., Stierhof, Y.-D., Hiller, U., Strompen, G., Bayer, M., Kientz, M., Sato, M.H., Nishimura, M.T., Dangl, J.L., Sanderfoot, A.A. and G. Jürgens (2018).
Concerted action of ancient and novel SNARE complexes in flowering-plant cytokinesis.
Developmental Cell, 44, 1-12.

Karnahl, M., Park, M., Krause, C., Hiller, U., Mayer, U., Stierhof, Y.-D., and G. Jürgens (2018).
Functional diversification of Arabidopsis SEC1-related SM proteins in cytokinetic and secretory membrane fusion.
Proceedings National Academic Sciences U.S.A., 115, 6309-6314.
doi/10.1073/pnas.1722611115 (May 29, Epub ahead of print.

Robinson, D.G., Hawes, C., Hillmer, S., Jürgens, G., Schwechheimer, C., Stierhof, Y.-D. and C. Viotti (2018).
Auxin and vesicle traffic. Questionable evidence for auxin-carrying secretory vesicles.
Plant Physiology, 176, 1884-1888.

Reipert, S., Goldammmer, H., Richardson, C., Hawkins, T., Goldberg, M., Hollergschwandtner, E., Antreich, S., Kaufmann, W. and Y.-D. Stierhof (2018).
Agitation modules: A flexible means to accelerate automatized freeze-substitution.
Journal of Histochemistry and Cytochemistry, 2018 Jul 1:22155418786698. doi: 10.1369/0022155418786698. (Epub ahead of print).


Dr. York Stierhof

York-Dieter Stierhof
Universität Tübingen
Zentrum für Molekularbiologie der Pflanzen ZMBP
Auf der Morgenstelle 32
D-72076 Tübingen / Germany

Tel: ++49-7071-29-76662 (Office)   Raum 3U09
-76663 (Lab)
-76661 (TEM)
-78088 (SEM)
Fax: ++49-7071-29-3287
Email: york.stierhofspam prevention@zmbp.uni-tuebingen.de


Sandra Richter

Universität Tübingen
Zentrum für Molekularbiologie der Pflanzen ZMBP
Auf der Morgenstelle 32
D-72076 Tübingen / Germany

Tel: ++49-7071-29-76662 (Office)

Fax: ++49-7071-29-3287

Email: sandra.richterspam prevention@zmbp.uni-tuebingen.de


Rebecca Stahl (80%)
Universität Tübingen
Zentrum für Molekularbiologie der Pflanzen ZMBP
Auf der Morgenstelle 32
D-72076 Tübingen / Germany

Tel: ++49-7071-29-76663 (Lab)
-76662 (Office)
-76661 (TEM)
Fax: ++49-7071-29-3287
email: rebecca.kuehnspam prevention@zmbp.uni-tuebingen.de


Diana Vranjkovic (SFB, 50%)

Universität Tübingen
Zentrum für Molekularbiologie der Pflanzen ZMBP
Auf der Morgenstelle 32
D-72076 Tübingen / Germany

Tel: ++49-7071-29-76663 (Lab)
-76662 (Office)

email: diana.vranjkovic@zmbp.uni-tuebingen.de