Research Group Grefen



Asseck L, Grefen C.

Detecting Interactions of Membrane Proteins: The Split-Ubiquitin System.

Methods in Molecular Biology, Two-Hybrid Systems, 1794:49-60

Vitali DG, Sinzel M, Bulthuis EP, Kolb A, Zabel S, Mehlhorn D, Costa BF, Farkas A, Clancy A, Schuldiner M, Grefen C, Schwappach B, Borgese N, Rapaport D.

The GET pathway can increase the risk of mitochondrial outer membrane proteins to be mistargeted to the ER.

Journal of Cell Science, early online

Asseck L, Wallmeroth N, Grefen C.

ER Membrane Protein Interactions Using the Split-Ubiquitin System (SUS).

Methods in Molecular Biology, The Plant Endoplasmic Reticulum, 1691:191-203

Mehlhorn D, Wallmeroth N, Berendzen KW, Grefen C.

2in1 Vectors Improve In Planta BiFC and FRET Analyses.

Methods in Molecular Biology, The Plant Endoplasmic Reticulum, 1691:139-158


De Col D, Fuchs P, Nietzel T, Elsässer M, Voon CP, Candeo A, Seeliger I, Fricker MD, Grefen C, Møller IM, Bassi A, Lim BL, Zancani M, Meyer AJ, Costa A, Wagner S, Schwarzländer M.

ATP sensing in living plant cells reveals tissue gradients and stress dynamics of energy physiology.

eLife 6:e26770

Xing S, Mehlhorn D, Wallmeroth N, Asseck L, Kar R, Voss A, Denninger P, Schmidt VAF, Schwarzländer M, Stierhof YD, Grossmann G, Grefen C.

Loss of GET pathway orthologues in Arabidopsis thaliana causes root hair growth defects and affects SNARE abundance.

Proc Natl Acad Sci USA 114:E1544–E1553

See Commentary in PNAS "Targeting tail-anchored proteins into plant organelles"


Xing S, Wallmeroth N, Berendzen KW, Grefen C.

Topical Review:

Techniques for the analysis of protein-protein interactions in vivo

Plant Physiology, 171: 727-58

*** article featured in the list Best Of 2016 in Plant Physiology ***


Albert I, Böhm H, Albert M, Feiler CE, Imkampe J, Wallmeroth N, Brancato C, Raaymakers TM, Oome S, Zhang H, Krol E, Grefen C, Gust AA, Chai J, Hedrich R, Van den Ackerveken G, Nürnberger T.

An RLP23–SOBIR1–BAK1 complex mediates NLP-triggered immunity.

Nature Plants, 1:15140

Grefen C, Karnik R, Larson E, Lefoulon C, Wang Y, Waghmare S, Zhang B, Hills A, Blatt MR.

A vesicle-trafficking protein commandeers Kv channel voltage sensors for voltage-dependent secretion.

Nature Plants, 1:15108

Hecker A, Wallmeroth N, Peter S, Blatt MR, Harter K, Grefen C.

Binary 2in1 vectors improve in planta (co-) localisation and dynamic protein interaction studies.

Plant Physiology, 168:776-87

Zhang B, Karnik R, Wang Y, Wallmeroth N, Blatt MR, Grefen C.

The R-SNARE VAMP721 Interacts with KAT1 and KC1 K+ Channels to Moderate K+ Current at the Plasma Membrane.

Plant Cell, 27:1697-717

Karnik R, Zhang B, Waghmare S, Aderhold C, Grefen C, Blatt MR.

Binding of SEC11 Indicates Its Role in SNARE Recycling after Vesicle Fusion and Identifies Two Pathways for Vesicular Traffic to the Plasma Membrane.

Plant Cell, 27:675-94


Lemonidis K, Gorleku OA, Sanchez-Perez MC,Grefen C, Chamberlain LH.

The Golgi S-acylation machinery is comprised of zDHHC enzymes with major differences in substrate affinity and S-acylation activity.

Mol Biol Cell, 25:3870-83

Lefoulon C, Karnik R, Honsbein A, Gutla PV, Grefen C, Riedelsberger J, Poblete T, Dreyer I, Gonzalez W, Blatt MR.

Voltage-sensor transitions of the inward-rectifying K+ channel KAT1 indicate a latching mechanism biased by hydration within the voltage sensor.

Plant Physiology, 166:960-75

Hachez C, Laloux T, Reinhardt H, Cavez D, Degand H, Grefen C, De Rycke R, Inzé D, Blatt MR, Russinova E, Chaumont F.

Arabidopsis SNAREs SYP61 and SYP121 Coordinate the Trafficking of Plasma Membrane Aquaporin PIP2;7 to Modulate the Cell Membrane Water Permeability.

Plant Cell, 26:3132-47

Singh MK, Krüger F, Beckmann H, Brumm S, Vermeer JE, Munnik T, Mayer U, Stierhof YD, Grefen C, Schumacher K, Jürgens G.

Protein delivery to vacuole requires SAND protein-dependent Rab GTPase conversion for MVB-vacuole fusion.

Current Biology, 24:1383-9

Grefen C.

The Split Ubiquitin System for the Analysis of Three-component Interactions.

Methods in Molecular Biology, Arabidopsis Protocols, 3rd Edition, 1062:659-78

Blatt MR, Grefen C.

Fluorescent Subcellular Markers in Arabidopsis.

Methods in Molecular Biology, Arabidopsis Protocols, 3rd Edition, 1062:487-507


Karnik R, Grefen C, Bayne R, Honsbein A, Köhler T, Kioumourtzoglou D, Williams M, Bryant N, Blatt MR.

Arabidopsis Sec1/Munc18 Protein SEC11 Is a Competitive and Dynamic Modulator of SNARE Binding and SYP121-Dependent Vesicle Traffic.

Plant Cell, 25:1368-82

Karnik A, Karnik R, Grefen C.

SDM-Assist - software to design site-directed mutagenesis primers introducing “silent” restriction sites.

BMC Bioinformatics, 14:105


Grefen C*, Blatt MR.

A 2in1 cloning system enables ratiometric Bimolecular Fluorescence Complementation (rBiFC).

Biotechniques, 53:311-14

Besserer A, Burnotte E, Bienert GP, Errachid A, Chevalier AS, Grefen C, Blatt MR, Chaumont F.

Selective regulation of maize plasma membrane aquaporin trafficking and activity by the SNARE ZmSYP121.

Plant Cell, 24:3463-81

Greaves J, Lemonidis K, Gorleku OA, Cruchaga C, Grefen C, Chamberlain LH.

Palmitoylation-Induced Aggregation of Cysteine-String Protein Mutants that cause Neuronal Ceroid Lipofuscinosis.

J Biol Chem, 287:37330-9

Grefen C, Blatt MR.

Do Calcineurin-B-like proteins interact independently of the serine threonine kinase CIPK23 with the K+-channel AKT1? Lessons learned from a ménage à trois.

Plant Physiology, 159:915-9

Eisenach C, Chen Z, Grefen C, Blatt MR.

The trafficking protein SYP121 of Arabidopsis connects programmed stomatal closure and K+ channel activity with vegetative growth.

Plant Journal, 69:241-51


Caesar K, Thamm AM, Witthöft J, Elgass K, Huppenberger P, Grefen C, Horak J, Harter K.

Evidence for the localization of the Arabidopsis cytokinin receptors AHK3 and AHK4 in the endoplasmic reticulum.

Journal of Experimental Botany, 62:5571-80

Grefen C, Honsbein A, Blatt MR.

Ion transport, membrane traffic and cellular volume control.

Current Opinion in Plant Biology, 14:332-9

Chen Z, Grefen C, Donald N, Hills A, Blatt MR.

A bicistronic, Ubiquitin-10 promoter-based vector cassette for transient transformation and functional analysis of membrane transport demonstrates the utility of quantitative voltage clamp studies on intact Arabidopsis root epidermis.

Plant Cell & Environment, 34:554-64

Honsbein A, Blatt MR., Grefen C.

A molecular framework for coupling cellular volume and osmotic solute transport control.

Journal of Experimental Botany, 62:2363-70


Grefen C, Chen Z, Honsbein A, Donald N, Hills A, Blatt MR.

A novel motif essential for SNARE interaction with the K+ channel KC1 and channel gating in Arabidopsis.

Plant Cell, 22:3076-92

Grefen C, Donald N, Hashimoto K, Kudla J, Schumacher K, Blatt MR.

A Ubiquitin-10 promoter-based vector set for fluorescent protein tagging facilitates temporal stability and native protein distribution in transient and stable expression studies.

Plant Journal, 64:355-65


Honsbein A, Sokolovski S, Grefen C, Campanoni P, Pratelli R, Paneque M, Chen Z, Johansson I, Blatt MR.

A Tripartite SNARE-K+ Channel Complex Mediates in Channel-Dependent K+ Nutrition in Arabidopsis.

Plant Cell, 21:2859-77

Grefen C, Obrdlik P, Harter K.

The determination of protein-protein interactions by the mating-based split-ubiquitin system (mbSUS).

Methods in Molecular Biology, Plant Signal Transduction, 479:217-33


Grefen C, Blatt MR.

SNAREs--molecular governors in signalling and development.

Current Opinion in Plant Biology, 11:600-9

Horák J, Grefen C, Berendzen KW, Hahn A, Stierhof YD, Stadelhofer B, Stahl M, Koncz C, Harter K.

The Arabidopsis thaliana response regulator ARR22 is a putative AHP phospho-histidine phosphatase expressed in the chalaza of developing seeds.

BMC Plant Biology, 8:77

Desikan R, Horák J, Chaban C, Mira-Rodado V, Witthöft J, Elgass K, Grefen C, Cheung MK, Meixner AJ, Hooley R, Neill SJ, Hancock JT, Harter K.

The histidine kinase AHK5 integrates endogenous and environmental signals in Arabidopsis guard cells.

PLoS One, 3:e2491

Grefen C, Städele K, Ruzicka K, Obrdlik P, Harter K, Horák J.

Subcellular Localization and In Vivo Interactions of the Arabidopsis thaliana Ethylene Receptor Family Members.

Molecular Plant, 1:308-20


Grefen C, Lalonde S, Obrdlik P.

Split-ubiquitin system for identifying protein-protein interactions in membrane and full-length proteins.

Current Protocols in Neuroscience, Chapter 5:Unit 5.27

Mira-Rodado V, Sweere U, Grefen C, Kunkel T, Fejes E, Nagy F, Schäfer E, Harter K.

Functional cross-talk between two-component and phytochrome B signal transduction in Arabidopsis.

Journal of Experimental Botany, 58:2595-607


Walter M, Chaban C, Schütze K, Batistic O, Weckermann K, Näke C, Blazevic D, Grefen C, Schumacher K, Oecking C, Harter K, Kudla J.

Visualization of protein interactions in living plant cells using bimolecular fluorescence complementation.

Plant Journal, 40:428-38

Grefen C, Harter K.

Plant two-component systems: principles, functions, complexity and cross talk.

Planta, 219:733-42

* first and corresponding author