LOCUS       pMZU-DEST          8731 bp    DNA     circular     28-MAY-2009
DEFINITION  mbSUS.
KEYWORDS    Split-Ubiquitin.
SOURCE      
  ORGANISM  
REFERENCE   
  AUTHORS   Christopher Grefen
FEATURES             Location/Qualifiers
     promoter        1..357
                     /label=adh
     exon            411..434
                     /label=c-myc
     misc_feature    465..589
                     /label=attR1
     CDS             698..1357
                     /label=CmR
     CDS             1699..2004
                     /label=ccdB
     misc_feature    complement(2045..2169)
                     /label=attR2
     rep_origin      complement(6168..6682)
                     /label=f1\ORI
     rep_origin      2579..3778
                     /label=2µ\ORI
     CDS             complement(4560..5363)
                     /label=URA3
     rep_origin      8263..8543
                     /label=pBR322\OR
     CDS             6803..7813
                     /label=SpecR
BASE COUNT     2503 a      1891 c      1912 g      2425 t 
ORIGIN
        1 tgttgtctca ccatatccgc aatgacaaaa aaatgatgga agacactaaa ggaaaaaatt 
       61 aacgacaaag acagcaccaa cagatgtcgt tgttccagag ctgatgaggg gtatctcgaa 
      121 gcacacgaaa ctttttcctt ccttcattca cgcacactac tctctaatga gcaacggtat 
      181 acggccttcc ttccagttac ttgaatttga aataaaaaaa agtttgctgt cttgctatca 
      241 agtataaata gacctgcaat tattaatctt ttgtttcctc gtcattgttc tcgttccctt 
      301 tcttccttgt ttctttttct gcacaatatt tcaagctata ccaagcatac aatcaactcc 
      361 aagctggatc ctctagacga gctcctcgag ccgccatcat ggaggagcag aagctgatct 
      421 cagaggagga cctgcatatg gccatggagg ccgaattccc atcaacaagt ttgtacaaaa 
      481 aagctgaacg agaaacgtaa aatgatataa atatcaatat attaaattag attttgcata 
      541 aaaaacagac tacataatac tgtaaaacac aacatatcca gtcactatgg cggccgcatt 
      601 aggcacccca ggctttacac tttatgcttc cggctcgtat aatgtgtgga ttttgagtta 
      661 ggatccgtcg agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata 
      721 taccaccgtt gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt 
      781 tgctcaatgt acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt 
      841 aaagaaaaat aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa 
      901 tgctcatccg gaattccgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt 
      961 tcacccttgt tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga 
     1021 ataccacgac gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg 
     1081 tgaaaacctg gcctatttcc ctaaagggtt tattgagaat atgtttttcg tctcagccaa 
     1141 tccctgggtg agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc 
     1201 ccccgttttc accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc 
     1261 gattcaggtt catcatgccg tttgtgatgg cttccatgtc ggcagaatgc ttaatgaatt 
     1321 acaacagtac tgcgatgagt ggcagggcgg ggcgtaaaga tctggatccg gcttactaaa 
     1381 agccagataa cagtatgcgt atttgcgcgc tgatttttgc ggtataagaa tatatactga 
     1441 tatgtatacc cgaagtatgt caaaaagagg tatgctatga agcagcgtat tacagtgaca 
     1501 gttgacagcg acagctatca gttgctcaag gcatatatga tgtcaatatc tccggtctgg 
     1561 taagcacaac catgcagaat gaagcccgtc gtctgcgtgc cgaacgctgg aaagcggaaa 
     1621 atcaggaagg gatggctgag gtcgcccggt ttattgaaat gaacggctct tttgctgacg 
     1681 agaacagggg ctggtgaaat gcagtttaag gtttacacct ataaaagaga gagccgttat 
     1741 cgtctgtttg tggatgtaca gagtgatatt attgacacgc ccgggcgacg gatggtgatc 
     1801 cccctggcca gtgcacgtct gctgtcagat aaagtctccc gtgaacttta cccggtggtg 
     1861 catatcgggg atgaaagctg gcgcatgatg accaccgata tggccagtgt gccggtctcc 
     1921 gttatcgggg aagaagtggc tgatctcagc caccgcgaaa atgacatcaa aaacgccatt 
     1981 aacctgatgt tctggggaat ataaatgtca ggctccctta tacacagcca gtctgcaggt 
     2041 cgaccatagt gactggatat gttgtgtttt acagcattat gtagtctgtt ttttatgcaa 
     2101 aatctaattt aatatattga tatttatatc attttacgtt tctcgttcag ctttcttgta 
     2161 caaagtggtt gatggggatc cgtcgacctg cagctgaagc tttggacttc ttcgccagag 
     2221 gtttggtcaa gtctccaatc aaggttgtcg gcttgtctac cttgccagaa atttacgaaa 
     2281 agatggaaaa gggtcaaatc gttggtagat acgttgttga cacttctaaa taagcgaatt 
     2341 tcttatgatt tatgattttt attattaaat aagttataaa aaaaataagt gtatacaaat 
     2401 tttaaagtga ctcttaggtt ttaaaacgaa aattcttatt cttgagtaac tctttcctgt 
     2461 aggtcaggtt gctttctcag gtatagcatg aggtcgctct tattgaccac acctctaccg 
     2521 gcatgcgata tgatccaata tcaaaggaaa tgatagcatt gaaggatgag actaatccaa 
     2581 ttgaggagtg gcagcatata gaacagctaa agggtagtgc tgaaggaagc atacgatacc 
     2641 ccgcatggaa tgggataata tcacaggagg tactagacta cctttcatcc tacataaata 
     2701 gacgcatata agtacgcatt taagcataaa cacgcactat gccgttcttc tcatgtatat 
     2761 atatatacag gcaacacgca gatataggtg cgacgtgaac agtgagctgt atgtgcgcag 
     2821 ctcgcgttgc attttcggaa gcgctcgttt tcggaaacgc tttgaagttc ctattccgaa 
     2881 gttcctattc tctagctaga aagtatagga acttcagagc gcttttgaaa accaaaagcg 
     2941 ctctgaagac gcactttcaa aaaaccaaaa acgcaccgga ctgtaacgag ctactaaaat 
     3001 attgcgaata ccgcttccac aaacattgct caaaagtatc tctttgctat atatctctgt 
     3061 gctatatccc tatataacct acccatccac ctttcgctcc ttgaacttgc atctaaactc 
     3121 gacctctaca ttttttatgt ttatctctag tattactctt tagacaaaaa aattgtagta 
     3181 agaactattc atagagtgaa tcgaaaacaa tacgaaaatg taaacatttc ctatacgtag 
     3241 tatatagaga caaaatagaa gaaaccgttc ataattttct gaccaatgaa gaatcatcaa 
     3301 cgctatcact ttctgttcac aaagtatgcg caatccacat cggtatagaa tataatcggg 
     3361 gatgccttta tcttgaaaaa atgcacccgc agcttcgcta gtaatcagta aacgcgggaa 
     3421 gtggagtcag gcttttttta tggaagagaa aatagacacc aaagtagcct tcttctaacc 
     3481 ttaacggacc tacagtgcaa aaagttatca agagactgca ttatagagcg cacaaaggag 
     3541 aaaaaaagta atctaagatg ctttgttaga aaaatagcgc tctcgggatg catttttgta 
     3601 gaacaaaaaa gaagtataga ttctttgttg gtaaaatagc gctctcgcgt tgcatttctg 
     3661 ttctgtaaaa atgcagctca gattctttgt ttgaaaaatt agcgctctcg cgttgcattt 
     3721 ttgttttaca aaaatgaagc acagattctt cgttggtaaa atagcgcttt cgcgttgcat 
     3781 ttctgttctg taaaaatgca gctcagattc tttgtttgaa aaattagcgc tctcgcgttg 
     3841 catttttgtt ctacaaaatg aagcacagat gcttcgttaa caaagatatg ctattgaagt 
     3901 gcaagatgga aacgcagaaa atgaaccggg gatgcgacgt gcaagattac ctatgcaata 
     3961 gatgcaatag tttctccagg aaccgaaata catacattgt cttccgtaaa gcgctagact 
     4021 atatattatt atacaggttc aaatatacta tctgtttcag ggaaaactcc caggttcgga 
     4081 tgttcaaaat tcaatgatgg gtaacaagta cgatcgtaaa tctgtaaaac agtttgtcgg 
     4141 atattaggct gtatctcctc aaagcgtatt cgaatatcat tgagaagctg catttttttt 
     4201 tttttttttt tttttttttt ttaaaaaaaa tttcaaggat ataccattgt aatgtctgcc 
     4261 cctaaaaaaa tcgtcgtttt gccaggggac cacgtttggt caaaaaatca cagccgaagc 
     4321 cattaaggtt cttaaagcta tttctgatgt tcgttccaag gtcaagttcg atttcaaaaa 
     4381 tcatttaatt gggggggctg ctatcgcaaa tccagatctg gctttttctt tccaattttt 
     4441 tttttttcgc cattataaaa atcattacga ccgaaattcc cgggtaataa ctgatataat 
     4501 taaattgaag ctctaatttg ggagtttagt atacatgcat ttacttataa tacagttttt 
     4561 tagttttgct ggccgcatct tctcaaatat gcttcccagc cggcttttct gtaacgttca 
     4621 ccctctacct tagcatccct tccctttgca aatagtcctc ttccaacaat aataatgtca 
     4681 gatcctgtag agaccacatc atccacggtt ctatactgtt gacccaatgc gtctcccttg 
     4741 tcatctaaac ccacaccggg tgtcataatc aaccaatcgt aaccttcatc tcttccaccc 
     4801 atgtctcttt gagcaataaa gccgataaca aaatctttgt cgctcttcgc aatgtcaaca 
     4861 gtacccttag tatattctcc agtagatagg gagcccttgc atgacaattc tgctaacatc 
     4921 aaaaggcctc taggttcctt tgttacttct tctgccgcct gcttcaaacc gctaacaata 
     4981 cctgggccca ccacaccgtg tgcattcgta atgtctgcgc attctgctat tctgtataca 
     5041 cccgcagagt actgcaattt gactgtatta ccaatgtcag caaattttct gtcttcgaag 
     5101 agtaaaaaat tgtacttggc ggataatgcc tttagcggct taactgtgcc ctccatggaa 
     5161 aaatcagtca agatatccac atgtgttttt agtaaacaaa ttttgggacc taatgcttca 
     5221 actaactcca gtaattcctt ggtggtacga acatccaatg aagcacacaa gtttgtttgc 
     5281 ttttcgtgca tgatattaaa tagcttggca gcaacaggac taggatgagt agcagcacgt 
     5341 tccttatatg tagctttcga catgatttat cttcgtttcc ggtttttgtt ctgtgcagtt 
     5401 gggttaagaa tactgggcaa tttcatgttt cttcaacact acatatgcgt atatatacca 
     5461 atctaagtct gtgctccttc cttcgttctt ccttctgttc ggagattacc gaatcaaaaa 
     5521 aatttcaagg aaaccgaaat caaaaaaaag aataaaaaaa aaatgatgaa ttgaaaagcc 
     5581 agatctgatc atctccgatg aagcctccgt tatcccaggc tccttgggtt tgttgccatc 
     5641 tgcgtccttg gcctctttgc cagacaagaa caccgcattt ggtttgtacg aaccatgcca 
     5701 tggttccgct ccagatttgc caaagaataa ggtcaaccct atcgccacta tcttgtctgc 
     5761 tgcaatgatg ttgaaattgt cattgaactt gcctgaagaa ggtaaagcca ttgaagatgc 
     5821 agttaaaaag gttttggatg caggtatcag aactggtgat ttaggtggtt ccaacagtac 
     5881 ccccgaagtc ggtgatgctg tcgccgaaga agttaagaaa atccttgctt aaaaagattc 
     5941 tcttttttta tgatatttgt ccaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 
     6001 aatgcagcgt cacatcggat aataatgatg gcagccattg tagaagtgcc ttttgcattt 
     6061 ctagtctctt tctcggtcta gctagtttta ctacatcgcg aagatagaat cttagatcac 
     6121 actgcctttg ctgagctgga tcaatagagt aacaaaagag tggtaaggac cccggttgat 
     6181 aatcagaaaa gccccaaaaa caggaagatt gtataagcaa atatttaaat tgtaagcgtt 
     6241 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag 
     6301 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt 
     6361 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga 
     6421 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg 
     6481 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct 
     6541 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc 
     6601 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt 
     6661 aatgcgccgc tacagggcgc gtcctcgtta aaggacaagg acctgagcgg aagtgtatcg 
     6721 tacagtagac ggagtatact agtaggcacg aacccagtgg acataagcct gttcggttcg 
     6781 taagctgtaa tgcaagtagc gtatgcgctc acgcaactgg tccagaacct tgaccgaacg 
     6841 cagcggtggt aacggcgcag tggcggtttt catggcttgt tatgactgtt tttttggggt 
     6901 acagtctatg cctcgggcat ccaagcagca agcgcgttac gccgtgggtc gatgtttgat 
     6961 gttatggagc agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa agttaaacat 
     7021 catgggggaa gcggtgatcg ccgaagtatc gactcaacta tcagaggtag ttggcgtcat 
     7081 cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg cagtggatgg 
     7141 cggcctgaag ccacacagtg atattgattt gctggttacg gtgaccgtaa ggcttgatga 
     7201 aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc ctggagagag 
     7261 cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca ttccgtggcg 
     7321 ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg 
     7381 tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa aagcaagaga 
     7441 acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg ttcctgaaca 
     7501 ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc ccgactgggc 
     7561 tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg 
     7621 caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc cggcccagta 
     7681 tcagcccgtc atacttgaag ctagacaggc ttatcttgga caagaagaag atcgcttggc 
     7741 ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg aaaggcgaga tcaccaaggt 
     7801 agtcggcaaa taatgtctag ctagaaattc gttcaagccg acgccgcttc gcggcgcggc 
     7861 ttaactcaag cgttagatgc actaagcaca taattgctca cagccaaact atcaggtcaa 
     7921 gtctgctttt attattttta agcgtgcata ataagcccta cacaaattgg gagatatatc 
     7981 atgcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga 
     8041 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac 
     8101 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt 
     8161 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc 
     8221 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat 
     8281 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag 
     8341 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc 
     8401 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag 
     8461 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac 
     8521 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg 
     8581 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct 
     8641 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgca tgcaacttct 
     8701 tttctttttt tttcttttct ctctcccccg t 
//
