LOCUS pMetOYC-Dest 10309 bp DNA circular 6-DEC-2012 DEFINITION mbSUS. KEYWORDS Split-Ubiquitin. SOURCE ORGANISM REFERENCE AUTHORS Christopher Grefen FEATURES Location/Qualifiers promoter 1..382 /label=met25 CDS 1743..2048 /label=ccdB misc_feature complement(2089..2213) /label=attR2 CDS 3426..3651 /label=VP16 CDS 2248..2376 /label=Cub CDS 2779..3384 /label=LexA CDS 2392..2766 /label=ProteinA rep_origin 5318..5997 /label=ARS1-ORI CDS complement(6520..7626) /label=LEU2 CDS 8231..9091 /label=AmpR centromere 4437..5288 /label=CEN4 rep_origin 9236..9909 /label=pUC CDS 742..1401 /label=CmR CDS 389..496 /label=Ost4 misc_recomb 509..633 /label=attR1 BASE COUNT 2892 a 2265 c 2350 g 2802 t ORIGIN 1 ggatgcaagg gttcgaatcc cttagctctc attatttttt gctttttctc ttgaggtcac 61 atgatcgcaa aatggcaaat ggcacgtgaa gctgtcgata ttggggaact gtggtggttg 121 gcaaatgact aattaagtta gtcaaggcgc catcctcatg aaaactgtgt aacataataa 181 ccgaagtgtc gaaaaggtgg caccttgtcc aattgaacac gctcgatgaa aaaaataaga 241 tatatataag gttaagtaaa gcgtctgtta gaaaggaagt ttttcctttt tcttgctctc 301 ttgtcttttc atctactatt tccttcgtgt aatacagggt cgtcagatac atagatacaa 361 ttctattacc cccatccata cactcgagat gatctctgat gaacagttga actccttggc 421 catcaccttc ggtattgtga tgatgacttt aattgtcatt taccatgctg ttgactccac 481 catgtctcct aagaacggtg gatctagaac aagtttgtac aaaaaagctg aacgagaaac 541 gtaaaatgat ataaatatca atatattaaa ttagattttg cataaaaaac agactacata 601 atactgtaaa acacaacata tccagtcact atggcggccg cattaggcac cccaggcttt 661 acactttatg cttccggctc gtataatgtg tggattttga gttaggatcc gtcgagattt 721 tcaggagcta aggaagctaa aatggagaaa aaaatcactg gatataccac cgttgatata 781 tcccaatggc atcgtaaaga acattttgag gcatttcagt cagttgctca atgtacctat 841 aaccagaccg ttcagctgga tattacggcc tttttaaaga ccgtaaagaa aaataagcac 901 aagttttatc cggcctttat tcacattctt gcccgcctga tgaatgctca tccggaattc 961 cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc 1021 gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc 1081 cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat 1141 ttccctaaag ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc 1201 accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg 1261 ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat 1321 gccgtttgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat 1381 gagtgggcag gcggggcgta aacgcgtgga tccggcttac taaaagccag ataacagtat 1441 gcgtatttgc gcgctgattt ttgcggtata agaatatata ctgatatgta tacccgaagt 1501 atgtcaaaaa gaggtatgct atgaagcagc gtattacagt gacagttgac agcgacagct 1561 atcagttgct caaggcatat atgatgtcaa tatctccggt ctggtaagca caaccatgca 1621 gaatgaagcc cgtcgtctgc gtgccgaacg ctggaaagcg gaaaatcagg aagggatggc 1681 tgaggtcgcc cggtttattg aaatgaacgg ctcttttgct gacgagaaca ggggctggtg 1741 aaatgcagtt taaggtttac acctataaaa gagagagccg ttatcgtctg tttgtggatg 1801 tacagagtga tattattgac acgcccgggc gacggatggt gatccccctg gccagtgcac 1861 gtctgctgtc agataaagtc tcccgtgaac tttacccggt ggtgcatatc ggggatgaaa 1921 gctggcgcat gatgaccacc gatatggcca gtgtgccggt ctccgttatc ggggaagaag 1981 tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg atgttctggg 2041 gaatataaat gtcaggctcc cttatacaca gccagtctgc aggtcgacca tagtgactgg 2101 atatgttgtg ttttacagca ttatgtagtc tgttttttat gcaaaatcta atttaatata 2161 ttgatattta tatcatttta cgtttctcgt tcagctttct tgtacaaagt ggtgattggt 2221 ggtggcggat ccggtggagg tggatcaggt atccctccag atcaacaaag attgatcttt 2281 gccggtaagc agctagaaga cggtagaacg ctgtctgatt acaacattca gaaggagtcc 2341 accttacatc ttgtgctaag gctaagaggt ggtatgcaca gatcagcttg cggccgcatg 2401 gcaggccttg cgcaacacga tgaagccgtg gacaacaaat tcaacaaaga acaacaaaac 2461 gcgttctatg agatcttaca tttacctaac ttaaacgaag aacaacgaaa cgccttcatc 2521 caaagtttaa aagatgaccc aagccaaagc gctaaccttt tagcagaagc taaaaagcta 2581 aatgatgctc aggcgccgaa agtagacaac aaattcaaca aagaacaaca aaacgcgttc 2641 tatgagatct tacatttacc taacttaaac gaagaacaac gaaacgcctt catccaaagt 2701 ttaaaagatg acccaagcca aagcgctaac cttttagcag aagctaaaaa gctaaatgat 2761 gctcaggcgc cgaaagtaga cgcgagcggc cgcatgaaag cgttaacggc caggcaacaa 2821 gaggtgtttg atctcatccg tgatcacatc agccagacag gtatgccgcc gacgcgtgcg 2881 gaaatcgcgc agcgtttggg gttccgttcc ccaaacgcgg ctgaagaaca tctgaaggcg 2941 ctggcacgca aaggcgttat tgaaattgtt tccggcgcat cacgcgggat ccgtctgttg 3001 caggaagagg aagaagggtt gccgctggta ggtcgtgtgg ctgccggtga accacttctg 3061 gcgcaacagc atattgaagg tcattatcag gtcgaccctt ccttattcaa gccgaatgct 3121 gatttcctgc tgcgcgtcag cgggatgtcg atgaaagata tcggcattat ggatggtgac 3181 ttgctggcag tgcataaaac tcaggatgta cgtaacggtc aggtcgttgt cgcacgtatt 3241 gatgacgagg ttaccgttaa gcgcctgaaa aaacagggca ataaagtcga actgttgcca 3301 gaaaatagcg agtttaaacc aattgtcgta gatcttcgtc agcagagctt caccattgaa 3361 gggctggcgg ttggggttat tcgcaacggc gactggctgg aattcccggg gatctgggcc 3421 cccccgaccg atgtcagcct gggggacgag ctccacttag acggcgagga cgtggcgatg 3481 gcgcatgccg acgcgctaga cgatttcgat ctggacatgt tgggggacgg ggattccccg 3541 ggtccgggat ttacccccca cgactccgcc ccctacggcg ctctggatat ggccgacttc 3601 gagtttgagc agatgtttac cgatgccctt ggaattgacg agtacggtgg gtagggggcg 3661 cgaccggacc cgcatccccc gtctgggttt tcccctcccg tccccggttc gtatccacaa 3721 taaacacgag cacatacatt acaaaaacct gcggttgtcg tctgattatt tggtggatcc 3781 gggggagatg ggggaggcta actgaaacac ggaaggagac aataccggaa ggaacccgcg 3841 ctatgacggc aataaaaaga cagaataaaa cgcacgggtg ttgggtcgtt tgttcataaa 3901 cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc ccattggggc 3961 caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg tgaaggccca 4021 gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag ccactggccc cgtgggttag 4081 ggacggggtc ccccatgggg aatggtttat ggttcgtggg ggttattatt ttgggcgttg 4141 cgtggggtca ggtccacgac ccaagctggc cgccaccgcg gtggagctcc aattcgccct 4201 atagtgagtc gtattacaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc 4261 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata 4321 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc 4381 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc ataatcgctg 4441 ggccattctc atgaagaata tcttgaattt attgtcatat tactagttgg tgtggaagtc 4501 ctaatatcgg tgatcaatat agtggttgac atgctggcta gtcaacattg agccttttga 4561 tcatgcaaat atattacggt attttacaat caaatatcaa acttaactat tgactttata 4621 acttatttag gtggtaacat tcttataaaa aagaaaaaaa ttactgcaaa acagtactag 4681 cttttaactt gtatcctagg ttatctatgc tgtctcacca tagagaatat tacctatttc 4741 agaatgtatg tccatgattc gccgggtaaa tacatataat acacaaatct ggcttaataa 4801 agtctataat atatctcata aagaagtgct aaattggcta gtgctatata tttttaagaa 4861 aatttctttt gactaagtcc atatcgactt tgtaaaagtt cacattagca tacatatatt 4921 acacgagcca gaaatagtaa cttttgccta aatcacaaat tgcaaaattt aattgcttgc 4981 aaaaggtcac atgcttataa tcaacttttt taaaaattta aaatactttt ttatttttta 5041 tttttaaaca taaatgaaat aatttattta ttgtttatga ttaccgaaac ataaaacctg 5101 ctcaagaaaa agaaactgtt ttgtccttgg aaaaaaagca ctacctagga gcggccaaaa 5161 tgccgaggct ttcatagctt aaactcttta cagaaaatag gcattataga tcagttcgag 5221 ttttcttatt cttccttccg gttttatcgt cacagtttta cagtaaataa gtatcacctc 5281 ttagagttcg atgataagct gtcaaacatg agaattaatt ccacatgtta aaatagtgaa 5341 ggagcatgtt cggcacacag tggaccgaac gtggggtaag tgcactaggg tccggttaaa 5401 cggatctcgc attgatgagg caacgctaat tatcaacata tagattgtta tctatctgca 5461 tgaacacgaa atctttactt gacgacttga ggctgatggt gtttatgcaa agaaaccact 5521 gtgtttaata tgtgtcactg tttgatatta ctgtcagcgt agaagataat agtaaaagcg 5581 gttaataagt gtatttgaga taagtgtgat aaagttttta cagcgaaaag acgataaata 5641 caagaaaatg attacgagga tacggagaga ggtatgtaca tgtgtattta tatactaagc 5701 tgccggcggt tgtttgcaag accgagaaaa ggctagcaag aatcgggtca ttgtagcgta 5761 tgcgcctgtg aacattctct tcaacaagtt tgattccatt gcggtgaaat ggtaaaagtc 5821 aaccccctgc gatgtatatt ttcctgtaca atcaatcaaa aagccaaatg atttagcatt 5881 atctttacat cttgttattt tacagatttt atgtttagat cttttatgct tgcttttcaa 5941 aaggcttgca ggcaagtgca caaacaatac ttaaataaat actactcagt aataacctat 6001 ttcttagcat ttttgacgaa atttgctatt ttgttagagt cttttacacc atttgtctcc 6061 acacctccgc ttacatcaac accaataacg ccatttaatc taagcgcatc accaacattt 6121 tctggcgtca gtccaccagc taacataaaa tgtaagctct cggggctctc ttgccttcca 6181 acccagtcag aaatcgagtt ccaatccaaa agttcacctg tcccacctgc ttctgaatca 6241 aacaagggaa taaacgaatg aggtttctgt gaagctgcac tgagtagtat gttgcagtct 6301 tttggaaata cgagtctttt aataactggc aaaccgagga actcttggta ttcttgccac 6361 gactcatctc catgcagttg gacgatcgat gataagctgt caaacatgag aattaattct 6421 accctatgaa catattccat tttgtaattt cgtgtcgttt ctattatgaa tttcatttat 6481 aaagtttatg tacaaatatc ataaaaaaag agaatctttt taagcaagga ttttcttaac 6541 ttcttcggcg acagcatcac cgacttcggt ggtactgttg gaaccaccta aatcaccagt 6601 tctgatacct gcatccaaaa cctttttaac tgcatcttca atggccttac cttcttcagg 6661 caagttcaat gacaatttca acatcattgc agcagacaag atagtggcga tagggttgac 6721 cttattcttt ggcaaatctg gagcagaacc gtggcatggt tcgtacaaac caaatgcggt 6781 gttcttgtct ggcaaagagg ccaaggacgc agatggcaac aaacccaagg aacctgggat 6841 aacggaggct tcatcggaga tgatatcacc aaacatgttg ctggtgatta taataccatt 6901 taggtgggtt gggttcttaa ctaggatcat ggcggcagaa tcaatcaatt gatgttgaac 6961 cttcaatgta ggaaattcgt tcttgatggt ttcctccaca gtttttctcc ataatcttga 7021 agaggccaaa acattagctt tatccaagga ccaaataggc aatggtggct catgttgtag 7081 ggccatgaaa gcggccattc ttgtgattct ttgcacttct ggaacggtgt attgttcact 7141 atcccaagcg acaccatcac catcgtcttc ctttctctta ccaaagtaaa tacctcccac 7201 taattctctg acaacaacga agtcagtacc tttagcaaat tgtggcttga ttggagataa 7261 gtctaaaaga gagtcggatg caaagttaca tggtcttaag ttggcgtaca attgaagttc 7321 tttacggatt tttagtaaac cttgttcagg tctaacacta cctgtacccc atttaggacc 7381 acccacagca cctaacaaaa cggcatcagc cttcttggag gcttccagcg cctcatctgg 7441 aagtggaaca cctgtagcat cgatagcagc accaccaatt aaatgatttt cgaaatcgaa 7501 cttgacattg gaacgaacat cagaaatagc tttaagaacc ttaatggctt cggctgtgat 7561 ttcttgacca acgtggtcac ctggcaaaac gacgatcttc ttaggggcag acattagaat 7621 ggtatatcct tgaaatatat atatatattg ctgaaatgta aaaggtaaga aaagttagaa 7681 agtaagacga ttgctaacca cctattggaa aaaacaatag gtccttaaat aatattgtca 7741 acttcaagta ttgtgatgca agcatttagt catgaacgct tctctattct atatgaaaag 7801 ccggttccgg cgctctcacc tttccttttt ctcccaattt ttcagttgaa aaaggtatat 7861 gcgtcaggcg acctctgaaa ttaacaaaaa atttccagtc atcgaatttg attctgtgcg 7921 atagcgcccc tgtgtgttct cgttatgttg aggaaaaaaa taatggttgc taagagattc 7981 gaactcttgc atcttacgat acctgagtat tcccacagtt aattcttgaa gacgaaaggg 8041 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc 8101 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca 8161 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa 8221 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt 8281 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca 8341 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag 8401 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc 8461 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca 8521 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt 8581 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct 8641 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt 8701 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga 8761 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact 8821 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc 8881 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga 8941 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt 9001 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga 9061 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact 9121 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga 9181 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt 9241 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca 9301 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct 9361 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta 9421 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct 9481 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc 9541 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca 9601 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga 9661 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg 9721 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt 9781 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag 9841 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt 9901 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt 9961 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga 10021 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta 10081 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa 10141 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat 10201 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta 10261 cgccaagctc gaaattaacc ctcactaaag ggaacaaaag ctgggtacc //