@article{Casini2023,
  author       = {Casini, Isabella and McCubbin, Tim and Esquivel-Elizondo, Sofia and
    Luque, Guillermo G. and Evseeva, Daria and Fink, Christian and Beblawy, Sebastian and
    Youngblut, Nicholas D. and Aristilde, Ludmilla and Huson, Daniel H. and Dr\"ager, Andreas
    and Ley, Ruth E. and Marcellin, Esteban and Angenent, Largus T. and Molitor, Bastian},
  title        = {{An integrated systems-biology approach reveals differences in formate metabolism
    in the genus \emph{Methanothermobacter}}},
  journal      = {iScience},
  pages        = {108016},
  year         = {2023},
  issn         = {2589-0042},
  doi          = {10.1016/j.isci.2023.108016},
  url          = {https://www.sciencedirect.com/science/article/pii/S258900422302093X},
  abstract     = {SUMMARY: Methanogenesis allows methanogenic archaea to generate cellular energy
    for their growth while producing methane. Thermophilic hydrogenotrophic species of the genus
    Methanothermobacter have been recognized as robust biocatalysts for a circular carbon economy
    and are already applied in power-to-gas technology with biomethanation, which is a platform to
    store renewable energy and utilize captured carbon dioxide. Here, we generated curated
    genome-scale metabolic reconstructions for three Methanothermobacter strains, and investigated
    differences in the growth performance of these same strains in chemostat bioreactor experiments
    with hydrogen and carbon dioxide or formate as substrates. Using an integrated systems biology
    approach, we identified differences in formate anabolism between the strains and revealed that
    formate anabolism influences the diversion of carbon between biomass and methane. This finding,
    together with the omics datasets and the metabolic models we generated, can be implemented for
    biotechnological applications of Methanothermobacter in power-to-gas technology, and as a
    perspective, for value-added chemical production.},
}