Interfakultäres Institut für Mikrobiologie und Infektionsmedizin

Publications

2018

  • Paul N. Schwarz, Luisa Roller, Andreas Kulik, Wolfgang Wohlleben, and Evi Stegmann, (2018) Engineering metabolic pathways in Amycolatopsis japonicum for the optimization of the precursor supply for heterologous brasilicardin congeners production. Synth. Syst. Biotechnol. 3:56-63.

2017

  • Paul N. Schwarz, Anina Buchmann, Luisa Roller, Andreas Kulik, Harald Gross, Wolfgang Wohlleben and Evi Stegmann, (2017) The immunosuppressant brasilicardin: Determination of the biosynthetic gene cluster in the heterologous host Amycolatopsis japonicum. Biotechnol. J., 10.1002/biot.201700527.
  • Tiia Kittilä, Claudia Kittel, Julien Tailhades, Diane Butz, Melanie Schoppet, Anita Büttner, Rob J. A. Goode, Ralf B. Schittenhelm, Karl-Heinz van Pee, Roderich D. Süssmuth, Wolfgang Wohlleben, Max J. Cryle* and Evi Stegmann*
    Halogenation of glycopeptide antibiotics occurs at the amino acid level during non-ribosomal peptide synthesis
    Chem. Sci., 2017,8, 5992-6004 DOI: 10.1039/C7SC00460E

2016

  • Anina Buchmann, Michael Eitel, Pierre Koch, Paul N. Schwarz, Evi Stegmann, Wolfgang Wohlleben, Marcin Wolański, Michał Krawiec, Jolanta Zakrzewska-Czerwińska, Carmen Méndez, Alma Botas, Luz Elena Núñez, Francisco Morís, Jesus Cortés, and Harald Gross
    High-Quality Draft Genome Sequence of the Actinobacterium Nocardia terpenica IFM 0406, Producer of the Immunosuppressant Brasilicardins, Using Illumina and PacBio Technologies
    Genome Announc. 2016 Nov-Dec; 4(6): e01391-16. DOI: 10.1128/genomeA.01391-16
  • Adamek M, Spohn M, Stegmann E, Ziemert N.
    Mining Bacterial Genomes for Secondary Metabolite Gene Clusters
    Methods Mol Biol. 2017;1520:23-47.
  • Kilian R, Frasch HJ, Kulik A, Wohlleben W, Stegmann E.
    The VanRS Homologous Two-Component System VnlRSAb of the Glycopeptide Producer Amycolatopsis balhimycina Activates Transcription of the vanHAXSc Genes in Streptomyces coelicolor, but not in A. balhimycina.
    Microb Drug Resist. 2016 Sep;22(6):499-509. doi: 10.1089/mdr.2016.0128.
  • Spohn M., Wohlleben W., Stegmann E. (2016). Elucidation of the zinc-dependent regulation in Amycolatopsis japonicum enabled the identification of the ethylenediamine-disuccinate ([S,S]-EDDS) genes. Environ. Microbiol. 18:1249-1263. doi: 10.1111/1462-2920.13159.
  • Iftime D., Jasyk M., Kulik A., Imhoff J. F., Stegmann E., Wohlleben W., Süssmuth R.D., Weber T. (2016). Streptocollin, a type IV lanthipeptide produced by Streptomyces collinus Tü 365. Chem. Bio. Chem. 16:2615-2623. doi: 10.1002/cbic.201500377.
  • Iftime D., Kulik A., Härtner T., Rohrer S., Niedermeyer T. H., Stegmann E., Weber T., Wohlleben W. (2016). Identification and activation of novel biosynthetic gene clusters by genome mining in the kirromycin producer Streptomyces collinus Tü 365. J. Ind. Microbiol. Biotechnol. 43:277-291. doi: 10.1007/s10295-015-1685-7.


2015

  • Medema M.H., Stegmann E., et. al. (2015) Minimum information about a biosynthetic gene cluster. Nat. Chem. Biol. 11:625-631. doi:10.1038/nchembio.1890.
  • Pozzi R., Coles M., Schwarz P., Linke D., Kulik A., Nega M., Wohlleben W., Stegmann E. (2015). Distinct mechanisms contribute to self-resistance in the lantibiotic NAI-107 producer strain Microbispora ATCC PTA-5024. Environ. Microbiol. doi: 10.1111/1462-2920.12892.
  • Frasch H. J., Kalan L., Kilian R., Kulik A., Wright G. C., Stegmann E. (2015). Innate pathway to a glycopeptide resistant cell wall. ACS Infec. Dis. doi: 10.1021/acsinfecdis.5b00011.


2014

  • Stegmann, E., Spohn M., Gert H., Weber T., Wohlleben W., Kalinowski J., Rückert C. (2014). Complete genome sequence of the actinobacterium Amycolatopsis japonica MG417-CF17T (=DSM 44213T) producing (S,S)-N,N'-ethylenediaminedisuccinic acid. J. Biotechnol. 189C:46-47.
  • Spohn, M., Kirchner N., Kulik A., Jochim A., Wolf F., Muenzer P., Borst O., Gross H., Wohlleben W., Stegmann E. (2014). Overproduction of ristomycin A by activation of a silent gene cluster in Amycolatopsis japonicum MG417-CF17. Antimicrob. Agents Chemother. 58:6185-96.
  • Sosio, M., Gallo G., Pozzi R., Serina S., Monciardini P., Bera A., Stegmann E., Weber T. (2014). Draft genome sequence of the Microbispora sp. strain ATCC-PTA-5024, producing the lantibiotic NAI-107. Genome Announc. 2. pii: e01198-13. doi: 10.1128/genomeA.01198-13.


2013

  • Towhid S. T., Tolios A., Münzer P., Schmidt E. M., Borst O., Gawaz M., Stegmann E., Lang F. (2013). Stimulation of platelet apoptosis by balhimycin. Biochem. Biophys. Res. Commun. 435:323-436.
  • Towhid S. T., Schmidt E. M., Tolios A., Münzer P., Schmid E., Borst O., Gawaz M., Stegmann E., Lang F. (2013). Stimulation of platelet death by vancomycin. Cell Physiol. Biochem. 31:102-112.


2012

  • Vongsangnak W., Figueiredo L. F., Förster J., Weber T., Thykaer J., Stegmann E., Wohlleben W., Nielsen J. (2012). Genome-scale metabolic representation of Amycolatopsis balhimycina. Biotechnol. Bioeng.109, 1798-807.


Before 2012

  • Schäberle T. F., Vollmer W., Frasch H. J., Hüttel S., Kulik A., Röttgen M., von Thaler A. K., Wohlleben W., Stegmann E. (2011). Self-resistance and cell wall composition in the glycopeptide producer Amycolatopsis balhimycina. Antimicrob. Agents Chemother. 55, 4283-4289.
  • Thykaer J., Nielsen J., Wohlleben W., Weber T., Gutknecht M., Lantz A. E., Stegmann E. (2010). Increased glycopeptide production after overexpression of shikimate pathway genes being part of the balhimycin biosynthetic gene cluster. Metab. Eng. 12, 455-61.
  • Gallo G., Renzone G., Alduina R., Stegmann E., Weber T., Eliasson A., Thykær J., Scaloni A., Puglia A. M. (2010). Differential proteomic analysis reveals novel links between primary metabolism and antibiotic production in Amycolatopsis balhimycina. Proteomics 10, 1336-1358.
  • Truman A. W., Fan Q., Röttgen M., Stegmann E., Leadlay P. F., Spencer J. B. (2008). The role of Cep15 in the biosynthesis of chloroeremomycin: reactivation of an ancestral catalytic function. Chem. Biol. 15, 476-84.
  • Chevillotte M., Menges. R., Muth G., Wohlleben W., Stegmann E. (2008). A quick and reliable method for monitoring gene expression in actinomycetes. J. Biotechnol. 135, 262-265.
  • Shawky R. M., Puk O., Wietzorrek A., Pelzer S., Takano E., Wohlleben W., Stegmann, E. (2007). The border sequence of the balhimycin biosynthesis gene cluster from Amycolatopsis balhimycina contains bbr, encoding a StrR-like pathway-specific regulator. J. Mol. Microbiol. Biotechnol. 13, 76-88.
  • Menges R., Muth G., Wohlleben W., Stegmann E. (2007). The ABC transporter Tba of Amycolatopsis balhimycina is required for efficient export of the glycopeptide antibiotic balhimycin. Appl. Microbiol. Biotechnol. 77, 125-134.
  • Hadatsch B., Butz D., Schmiederer T., Steudle J., Wohlleben W., Süssmuth R. D. Stegmann E. (2007). The biosynthesis of teicoplanin-type glycopeptide antibiotics: assignment of P450 mono-oxygenases to side chain cyclisations of glycopeptide A47934. Chem. Biol. 14, 1078-1089.
  • Stegmann E., Rausch C., Stockert S., Burkert D., Wohlleben W. (2006). The small MbtH-like protein encoded by an internal gene of the balhimycin biosynthetic gene cluster is not required for glycopeptide production. FEMS Microbiol. Lett. 262, 85-92.
  • Stegmann E., Pelzer S., Bischoff D., Puk O., Stockert S., Butz D., Zerbe K., Robinson J., Süssmuth R. D., Wohlleben, W. (2006). Genetic analysis of the balhimycin (vancomycin-type) oxygenase genes. J. Biotechnol. 124, 640-653.
  • Donadio S., Sosio M., Stegmann E., Weber T., Wohlleben, W. (2005). Comparative analysis and insights into the evolution of gene clusters for glycopeptide biosynthesis. Mol. Gen. Genet. 274, 40-50.
  • Bischoff D., Bister B., Bertazzo M., Pfeifer V., Stegmann E., Nicholson G. J., Keller S., Pelzer S., Wohlleben W., Süssmuth R. D. (2005). The biosynthesis of vancomycin-type glycopeptide antibiotics - a model for oxidative side-chain cross-linking by oxygenases coupled to the action of peptide synthetases. Chem. Bio. Chem. 6, 2267-2272.
  • Puk O., Bischoff D., Kittel C., Pelzer S., Weist S., Stegmann E., Süssmuth R., Wohlleben W. (2004). Biosynthesis of chloro-b-hydroxytyrosine, a non-proteinogenic amino acid of the peptidic backbone of vancomycin-type glycopeptide antibiotics. J. Bacteriol. 186, 6093-6100.
  • Stegmann E., Pelzer S., Wilken K., Wohlleben W., (2001). Development of three different gene cloning systems for genetic investigation of the new species Amycolatopsis japonicum MG417-CF17, the ethylenediaminedisuccinic acid (EDDS) producer. J. Biotechnol. 92, 195-204.

Reviews

  • Wohlleben W, Mast Y, Stegmann E, Ziemert N.
    Anitbiotic drug discovery.
    Microb Biotechnol. 2016 Sep;9(5):541-8. doi: 10.1111/1751-7915.12388.
  • Stegmann E., Frasch H. J., Kilian R., Pozzi R. (2015). Self-resistance mechanisms of actinomycetes producing lipid II-targeting antibiotics. Int. J. Med. Microbiol. 305:190-195. doi: 10.1016/j.ijmm.2014.12.015.
  • Wohlleben W., Mast Y., Muth G., Röttgen M., Stegmann E., Weber T. (2012) Synthetic biology of secondary metabolite biosynthesis in actinomycetes: Engineering precursor supply as a way to optimize antibiotic production. FEBS Lett. 586, 2171-2176.
  • Stegmann E., Frasch H. J., Wohlleben W. (2010) Glycopeptide biosynthesis in the context of basic cellular functions. Curr Opin Microbiol. 13, 595-602.
  • Wohlleben W., Stegmann E., Süssmuth, R. D. (2009). Chapter 18: Molecular Genetic Approaches to Analyse Glycopeptide Biosynthesis. Meth. Enzymol. 458, 459-486.
  • Stegmann E., Bischoff D., Kittel C., Pelzer S., Puk O., Recktenwald J., Weist S., Süssmuth R., Wohlleben W. (2005). Precursor-directed biosynthesis for the generation of novel glycopeptides. Ernst Schering Res. Found. Workshop. 51, 215-232.
  • Stegmann E., Hadatsch B., Kittel C., Puk O., Menges R., Suessmuth R., Wohlleben, W. (2001). Interlocking of primary and secondary metabolism in antibiotic-producing actinomycetes. Chemical Engeneering Transactions 14, 161-167.

Patent application

  • Stegmann E., Spohn M., Weber T., Wohlleben W., (2013) [S,S]-EDDS-Biosynthesegene und –proteine und Verfahren zur Biosynthese von [S,S]-EDDS. P 53563 DE.