Pharmaceutical Biotechnology and Antibiotic Discovery
Leipoldt F, Zeyhle P, Kulik A, Kalinowski J, Heide L, Kaysser L (2015) Diversity of ABBA Prenyltransferases in Marine Streptomyces sp. CNQ-509: Promiscuous Enzymes for the Biosynthesis of Mixed Terpenoid Compounds. PLoS One 10: e0143237
Rückert C, Leipoldt F, Zeyhle P, Fenical W, Jensen PR, Kalinowski J, Heide L, Kaysser L (2015) Complete genome sequence of Streptomyces sp. CNQ-509, a prolific producer of meroterpenoid chemistry. J Biotechnol 216: 140-141
Zeyhle P, Bauer JS, Steimle M, Leipoldt F, Rösch M, Kalinowski J, Gross H, Heide L (2014) A membrane-bound prenyltransferase catalyzes the O-prenylation of 1,6-dihydroxyphenazine in the marine bacterium Streptomyces sp. CNQ-509. Chembiochem 15: 2385-2392
Zeyhle P, Bauer JS, Kalinowski J, Shin-ya K, Gross H, Heide L (2014) Genome-based discovery of a novel membrane-bound 1,6-dihydroxyphenazine prenyltransferase from a marine actinomycete. PLoS One 9: e99122
Zettler J, Xia H, Burkard N, Kulik A, Grond S, Heide L, Apel AK (2014) New aminocoumarins from the rare actinomycete Catenulispora acidiphila DSM 44928: identification, structure elucidation, and heterologous production. Chembiochem 15: 612-621
Rückert C, Kalinowski J, Heide L, Apel AK (2014) Draft Genome Sequence of Streptomyces roseochromogenes subsp. oscitans DS 12.976, Producer of the Aminocoumarin Antibiotic Clorobiocin. Genome Announc 2: e01147-13
Flinspach K, Rückert C, Kalinowski J, Heide L, Apel AK (2014) Draft Genome Sequence of Streptomyces niveus NCIMB 11891, Producer of the Aminocoumarin Antibiotic Novobiocin. Genome Announc 2: e01146-13
Bonitz T, Zubeil F, Grond S, Heide L (2014) Unusual N-prenylation in diazepinomicin biosynthesis: the farnesylation of a benzodiazepine substrate is catalyzed by a new member of the ABBA prenyltransferase superfamily. PLoS One 8: e85707
Jones AC, Gust B, Kulik A, Heide L, Buttner MJ, Bibb MJ (2013) Phage p1-derived artificial chromosomes facilitate heterologous expression of the FK506 gene cluster. PLoS One 8: e69319
Novotna J, Gust B, Kulik A, Spizek J, Heide L (2013) Five gene products are required for assembly of the central pyrrole moiety of coumermycin A1. J Ind Microbiol Biotechnol 40: 915-925
Boll B, Heide L (2013) A Domain of RubC1 of Rubradirin Biosynthesis Can Functionally Replace MbtH-Like Proteins in Tyrosine Adenylation. Chembiochem 14: 43-44
Herbst DA, Boll B, Zocher G, Stehle T, Heide L (2013) Structural Basis of the Interaction of MbtH-Like Proteins, Putative Regulators of Non-Ribosomal Peptide Biosynthesis, with Adenylating Enzymes. J Biol Chem. 288: 1991-2003
Zocher G, Saleh O, Heim JB, Herbst DA, Heide L, Stehle T. (2012) Structure-based engineering increased the catalytic turnover rate of a novel phenazine prenyltransferase. PLoS One 7 (10): e48427.
Saleh O, Bonitz T, Flinspach K, Kulik A, Burkard N, Mühlenweg A, Vente A, Polnick S, Lämmerhofer M, Gust B, Fiedler HF, Heide L (2012) Activation of a silent phenazine biosynthetic gene cluster reveals a novel natural product and a new resistance mechanism against phenazines. MedChemComm 3: 1009-1019
Saleh O, Flinspach K, Westrich L, Kulik A, Gust B, Fiedler HP, Heide L (2012) Mutational analysis of a phenazine biosynthetic gene cluster in Streptomyces anulatus 9663. Beilstein J Org Chem 8: 501-513
Bonitz T, Alva V, Saleh O, Lupas AN, Heide L (2011) Evolutionary relationships of microbial aromatic prenyltransferases. PLoS One. 6(11):e27336.
Siebenberg S, Burkard N, Knuplesch A, Gust B, Grond S, Heide L (2011) Two pathways for pyrrole formation in coumermycin A1 biosynthesis: the central pyrrole moiety is formed from L-threonine. Chembiochem 12: 2677-2685
Boll B, Taubitz T, Heide L (2011) The role of MbtH-like proteins in the adenylation of tyrosine during aminocoumarin and vancomycin biosynthesis. J. Biol. Chem. 286: 36281-36290.
Alt S, Mitchenall L, Maxwell A, Heide L: Inhibition of DNA gyrase and DNA topoisomerase IV of Staphylococcus aureus and Escherichia coli by aminocoumarin antibiotics (2011) J Antimicrob Chemother 66: 2061-2069
Boll B, Hennig S, Xie C, Kyong Sohng J, Heide L (2011) Adenylate-forming enzymes of rubradirin biosynthesis: RubC1 is a bifunctional enzyme with aminocoumarin acyl ligase and tyrosine-activating domains. Chembiochem 12: 1105 – 1114
Alt S, Burkard N, Kulik A, Grond S, Heide L (2011) An artificial pathway to 3,4-dihydroxybenzoic acid allows generation of a new aminocoumarin antibiotic recognized by catechol transporters of E. coli. Chem Biol 18: 304-313
Siebenberg S, Kaysser L, Wemakor E, Heide L, Gust B, Kammerer B (2011) Identification and structural elucidation of new caprazamycins from Streptomyces sp. MK730-62F2 by liquid chromatography/electrospray ionization tandem mass spectrometry. Rapid Commun Mass Spectrom. 25: 495-502
Seeger K, Flinspach K, Haug-Schifferdecker E, Kulik A, Gust B, Fiedler HP, Heide L (2010) The biosynthetic genes for prenylated phenazines are located at two different chromosomal loci of Streptomyces cinnamonensis DSM 1042. Microb Biotechnol 4:252-62
Metzger U, Keller S, Stevenson CE, Heide L, Lawson DM (2010) Structure and mechanism of the magnesium-independent aromatic prenyltransferase CloQ from the clorobiocin biosynthetic pathway. J Mol Biol 404: 611-626
Flinspach K, Westrich L, Kaysser L, Siebenberg S, Gomez- Escribano JP, Bibb M, Gust B, Heide L (2010) Heterologous expression of the biosynthetic gene clusters of coumermycin A1, clorobiocin and caprazamycins in genetically modified Streptomyces coelicolor strains. Biopolymers 93: 823-832
Haug-Schifferdecker E, Arican D, Brückner R, Heide L (2010) A new group of aromatic prenyltransferases in fungi, catalysing a 2,7-dihydroxynaphthalene 3-dimethylallyl- transferase reaction. J Biol Chem 285: 16487-16494
Zhao X, GustB, Heide L (2010) S-Adenosylmethionine (SAM) and antibiotic biosynthesis: Effect of external addition of SAM and of overexpression of SAM biosynthesis genes on novobiocin production in Streptomyces. Arch Microbiol 192:289–297
Dangel V, Westrich L, Smith MCM, Heide L, Gust B (2010) Use of an inducible promoter for antibiotic production in a heterologous host. Appl Microbiol Biotechnol 87: 261-269
Siebenberg S, Bapat PM, Lantz AE, Gust B, Heide L (2010) Reducing the variability of antibiotic production in Streptomyces by cultivation in 24-square deepwell plates. J Biosci Bioeng 109: 230-234
Mavrodi DV, Tobin L. Peever TL, Mavrodi OV, Parejko JA, Raaijmakers JM, Lemanceau P, Mazurier S, Heide L, Blankenfeldt W, Weller DM, Thomashow LS (2010) Diversity and evolution of the phenazine biosynthesis pathway. Appl Env Microbiol, 76:866-879
Dangel V, Härle J, Goerke C, Wolz C, Gust B, Pernodet JL, Heide L (2009) Transcriptional regulation of the novobiocin biosynthetic gene cluster. Microbiology 155: 4025-4035
Metzger U, Schall C, Zocher G, Unsöld I, Stec E, Li SM, Heide L, Stehle T (2009) The structure of dimethylallyl tryptophan synthase reveals a common architecture of aromatic prenyltransferases in fungi and bacteria. Proc Nat Acad Sci (USA) 106:14309-14314
Saleh O, Gust B, Boll B, Fiedler HP, Heide L (2009) Aromatic prenylation in phenazine biosynthesis: dihydrophenazine-1-carboxylate dimethylallyltransferase from Streptomyces anulatus. J Biol Chem 284:14439-14447
Dangel V, Eustáquio AS, Gust B, Heide L (2008)novE and novG act as positive regulators of novobiocin biosynthesis. Arch. Microbiol. 190: 509-519.
Heide L, Westrich L, Anderle C, Gust B, Kammerer B, Piel J (2008) Use of a halogenase of hormaomycin biosynthesis for formation of new clorobiocin analogs with 5-chloro-pyrrole moieties. ChemBioChem 9:1992-1999.
Anderle C, Stieger M, Burrell M, Reinelt S, Maxwell A, Page M, Heide L (2008) Biological activities of novel gyrase inhibitors of the aminocoumarin class. Antimicrob Agents Chemother 52: 1982–1990.
Wolpert M, Heide L, Kammerer B, Gust B (2008) Assembly and heterologous expression of the coumermycin A1 gene cluster and production of new derivatives by genetic engineering. ChemBioChem 9: 603-612.
Anderle C, Li S-M, Kammerer B, Gust B, Heide L (2007) New aminocoumarin antibiotics derived from 4-hydroxycinnamic acid are formed after heterologous expression of a modified clorobiocin biosynthetic gene cluster. J Antibiot (Tokyo). 60: 504-510.
Anderle C, Hennig S, Kammerer B, Li S-M, Wessjohann L, Gust B, Heide L (2007) Improved mutasynthetic approaches for the production of modified aminocoumarin antibiotics. Chem & Biol 14: 955–967
Haagen Y, Unsöld I, Westrich L, Gust B, Heide L (2007) A soluble, magnesium-independent prenyltransferase catalyzes reverse and regular C-prenylations and O-prenylations of aromatic substrates. FEBS Letters 581: 2889-2893
Bringmann G, Haagen Y, Gulder TAM, Gulder T, Heide L (2007) Biosynthesis of the isoprenoid moieties of furanonaphthoquinone I and endophenazine A in Streptomyces cinnamonensis DSM 1042. J Org Chem 72: 4198-4204.
Anderle C, Alt A, Gulder T, Bringmann G, Kammerer B, Gust B and Heide L (2007) Biosynthesis of clorobiocin: Investigation of the transfer and methylation of the pyrrolyl-2-carboxyl moiety. Arch Microbiol 187, 227-237.
Wolpert M, Gust B, Kammerer B, Heide L (2007) Effects of deletions of mbtH-like genes on clorobiocin biosynthesis in Streptomyces coelicolor. Microbiology 153: 1413-1423
Haagen Y, Glück K, Fay K, Kammerer B, Gust B, Heide L (2006) A gene cluster for prenylated naphthoquinone and prenylated phenazine biosynthesis in Streptomyces cinnamonensis DSM 1042. Chembiochem 7: 2016-2027
Keller S, Pojer F, Heide L, Lawson DM (2006) Molecular replacement in the "twilight zone": crystal structure determination of the non-heme iron oxygenase NovR from Streptomyces spheroides through repeated density modification of a poor molecular replacement solution. Acta Cryst D 62:1564-1570
Keller S, Pojer F, Heide L, Lawson DM (2006) Crystallization and preliminary X-ray analysis of the aromatic prenyltransferase CloQ from the clorobiocin biosynthetic cluster of Streptomyces roseochromogenes. Acta Cryst. F 62, 1153–1155
Freitag, A., Méndez, C., Salas, J.A., Kammerer, B., Li, S.-M., Heide, L (2006). Metabolic engineering of the heterologous production of clorobiocin derivatives and elloramycin in Streptomyces coelicolor M512. Metab Eng 8: 653-661
Freitag A, Li S-M, Heide L (2006) Biosynthesis of the unusual 5,5-gem-dimethyl-deoxysugar noviose: investigation of the C-methyltransferase gene cloU. Microbiology 152: 2433-2442
Flatman RH, Eustaquio A, Li S-M, Heide L, Maxwell A (2006) Structure-activity relationships of aminocoumarin-type gyrase and topoisomerase iv inhibitors obtained by combinatorial biosynthesis. Antimicrob Agents Chemother 50: 1136–1142
Kammerer B, Kahlich R, Biegert C, Gleiter CH, Heide L (2005) HPLC-MS/MS analysis of willow bark extracts contained in pharmaceutical preparations. Phytochem Anal 16: 470-478
Freitag A, Wemakor E, Li S-M, Heide L (2005) Acyl transfer in clorobiocin biosynthesis: Involvement of several proteins in the transfer of the pyrrole-2-carboxyl moiety to the deoxysugar. ChemBioChem 6: 2316-2325
Luft T, Li S-M, Scheible H, Kammerer B, Heide L (2005) Overexpression, purification and characterization of SimL, an amide synthetase involved in simocyclinone biosynthesis. Arch Microbiol 183: 277-285
Eustáquio AS, Li S-M, Heide L (2005) NovG, a DNA-binding protein acting as a positive regulator of novobiocin biosynthesis. Microbiology 151: 1949-1961.
Freitag A, Rapp H, Heide L, Li S-M (2005) Metabolic engineering of aminocoumarins: Inactivation of the methyltransferase gene cloP and generation of new clorobiocin derivatives in a heterologous host. ChemBioChem 6: 1411-1418
Eustáquio AS, Gust B, Galm U, Li S-M, Chater KF, Heide L (2005) Heterologous expression of the biosynthetic gene clusters of novobiocin and clorobiocin. Appl Environ Microbiol 71: 2452-2459
Thuy TTT, Lee HC, Kim C-G, Heide L, Sohng JK (2005) Functional characterizations of novWUS involved in novobiocin biosynthesis from Streptomyces spheroides. Arch Biochem Biophys 436: 161-176
Flatman RH, Howells AJ, Heide L, Fiedler H-P, Maxwell A (2005) Simocyclinone D8: an inhibitor of DNA gyrase with a novel mode of action. Antimicrob Agents Chemother 49: 1093-1100
Freel Meyers CL, Oberthür M, Heide L, Kahne D, Walsh CT (2004) Assembly of dimeric variants of coumermycins by tandem action of the four biosynthetic enzymes CouL, CouM, CouP, and NovN. Biochemistry 43: 15022-15036
Eustáquio AS, Gust B, Li S-M, Pelzer S, Wohlleben W, Chater KF, Heide L (2004) Production of 8´-halogenated and 8´-unsubstituted novobiocin derivatives in genetically engineered Streptomyces coelicolor strains. Chem & Biol 11: 1561-1572.
Kammerer B, Kahlich R, Laufer S, Li SM, Heide L, Gleiter CH (2004) Mass spectrometric pathway monitoring of secondary metabolites - Systematic analysis of culture extracts of Streptomyces species. Anal Biochem 335, 17-29
Biegert C, Wagner I, Lüdtke R, Kötter I, Lohmüller C, Günaydin I, Taxis K, Heide L (2004) Efficacy and safety of willow bark extract in the treatment of osteoarthritis and rheumatoid arthritis. Results of two randomized double-blind controlled trials. J Rheumatol 31, 2121-2130
Xu H, Heide L, Li S-M (2004) New aminocoumarin antibiotics formed by a combined mutational and chemoenzymatic approach utilizing the carbamoyltransferase NovN. Chem & Biol 11: 655-662
Freitag A, Galm U, Li S.-M, Heide L (2004). New aminocoumarin antibiotics from a cloQ-defective mutant of the clorobiocin producer Streptomyces roseochromogenes DS12.976. J Antibiotics 57: 205-210
Galm U, Heller S, Shapiro S, Page M, Li S-M, Heide L (2004) Antimicrobial and DNA gyrase-inhibitory activities of novel clorobiocin derivatives prepared by mutasynthesis. Antimicrob Agents Chemother. 48: 1307-1312
Galm U, Dessoy MA, Schmidt J, Wessjohann LA, Heide L (2004) In vitro and in vivo production of new aminocoumarins by a combined biochemical, genetic and synthetic approach. Chem & Biol 11: 173-183
Schmutz E, Hennig S, Li S-M, Heide L (2004) Identification of a topoisomerase IV in actinobacteria: purification and characterization of ParYR and GyrBR from the coumermycin A1 producer Streptomyces rishiriensis DSM 40489. Microbiology 150: 641-647
Freel Meyers CL, Oberthür M, Xu H, Heide L, Kahne D, Walsh CT (2004) Characterization of NovP and NovN: Completion of novobiocin biosynthesis by sequential tailoring of the noviosyl ring. Angew Chem Int Ed Engl, 43: 67-70.
Schmutz E, Steffensky M, Schmidt J, Porzel A, Li S-M, Heide L (2003) An unusual amide synthetase (CouL) from the coumermycin A1 biosynthetic gene cluster from Streptomyces rishiriensis DSM 40489. Eur J Biochem 270: 4413-4419
Pojer F, Kahlich R, Kammerer K, Li S-M, Heide L (2003) CloR, a bifunctional non-heme iron oxygenase involved in clorobiocin biosynthesis. J Biol Chem 278:30661-30668.
Westrich L, Heide L, Li S-M (2003) CloN6, a novel methyltransferase catalysing the methylation of the pyrrole-2-carboxyl moiety of clorobiocin. ChemBiochem 4: 100-106.
Xu H, Kahlich R, Kammerer B, Heide L, Li S-M (2003) CloN2, a novel acyltransferase involved in the attachment of the pyrrole-2-carboxyl moiety to the deoxysugar of clorobiocin. Microbiology 149: 2183-2191
Eustáquio A, Luft T, Wang Z-X, Gust B, Chater KF, Li S-M, Heide L (2003) Novobiocin biosynthesis: inactivation of the putative regulatory gene novE and heterologous expression of genes involved in aminocoumarin ring formation. Arch. Microbiol. 180: 25-32
Eustáquio A, Gust B, Luft T, Li S-M, Chater K, Heide L (2003) Clorobiocin biosynthesis in Streptomyces: Identification of the halogenase and generation of structural analogues. Chem. & Biol. 10: 279-88.
Wagner I, Heide L (2003) Effects of willow bark extract (Letter). Clin. Pharmacol. Ther. 74: 96-97
Wagner I, Greim C, Laufer S, Heide L, Gleiter CH (2003) Influence of willow bark extract on cyclooxygenase activity and on tumor necrosis factor α or interleukin 1ß release in vitro and ex vivo. Clin. Pharmacol. Ther. 73: 272-274
Pojer F, Wemakor E, Kammerer B, Chen H, Walsh CT, Li S-M, Heide L (2003) CloQ, a prenyltransferase involved in clorobiocin biosynthesis. Proc. Natl. Acad. Sci. (USA) 100: 2316-2321
Schmutz E, Mühlenweg A, Li S-M, Heide L (2003) Resistance genes of aminocoumarin producers: Two type II topoisomerase genes confer resistance against coumermycin A1 and clorobiocin. Antimicrob. Agents Chemother. 47: 869-877
Köhle A, Sommer S, Li S-M, Schilde-Rentschler L, Ninnemann H, Heide L (2003) Secondary metabolites in transgenic tobacco and potato: high accumulation of 4-hydroxybenzoic acid glucosides result from high expression of the bacterial gene ubiC. Molecular Breeding 11:15-24
Wagner I, Heide L (2003) Salicin and treatment of rheumatic diseases (Letter). J Rheumatol 30, 1125
Xu H, Wang Z-X, Schmidt J, Heide L, Li S-M (2002) Genetic analysis of the biosynthesis of the pyrrole and carbamoyl moieties of coumermycin A1 and novobiocin. Mol. Genet. Genomics 268: 387-396
Li S-M, Westrich L, Schmidt J, Kuhnt C, Heide L (2002) Methyltransferase genes in Streptomyces rishiriensis: new coumermycin derivatives from gene-inactivation experiments. Microbiology 148: 3317-3326
Pojer F, Li S-M, Heide L (2002) Identification of the clorobiocin biosynthetic gene cluster. New insights into the biosynthesis of aminocoumarin antibiotics. Microbiology 148: 3901-3911
Köhle A, Sommer S, Yazaki K, Ferrer A, Boronat A, Li S-M, Heide L (2002) High level expression of chorismate pyruvate-lyase (UbiC) and HMG-CoA reductase in hairy root cultures of Lithospermum erythrorhizon. Plant & Cell Phys. 43: 894-902
Galm U, Schimana J, Fiedler H-P, Schmidt J, Li S-M, Heide L (2002) Cloning and analysis of the simocyclinone biosynthetic gene cluster of Streptomyces antibioticus Tü 6040. Arch Microbiol, 178: 102-114
Schmid B, Kötter I, Heide L (2001) Pharmacokinetics of salicin after oral application of a standardized willow bark extract. Eur. J. Clin. Pharmacol. 57, 387-391.
Deutsche Fassung: Schmid B, Biegert C, Kötter I, Heide L (2002) Pharmakokinetik von Salicin nach oraler Gabe eines standardisierten Weidenrindenextraktes. In: Phytopharmaka VII (Schultz V, Rietbrock N, Roots I, Loew D, Eds), Steinkopf, Darmstadt, Germany. pp. 93-100.
Li S-M, Wemakor E, Heide L (2001) PCR amplification of 1-deoxy-D-xylulose 5-phosphate synthase (dxs) genes from different Streptomyces species: Evidence for the existence of two dxs gene families. Molecular Biology Today 2: 61-66
Schmid B, Lüdtke R, Selbmann H-K, Schaffner W, Kötter I, Tschirdewahn B, Heide L (2001) Efficacy and tolerability of a standardized willow bark extract in patients with osteoarthritis: randomized placebo-controlled, double blind clinical trial; Phytother. Res. 15, 1-7
Wang Z-X, Li S-M, Heide L (2000) Identification of the coumermycin A1 biosynthetic gene cluster of Streptomyces rishiriensis DSM 40489; Antimicrob. Agents Chemother. 44, 3040-3048
Steffensky M, Li S-M, Heide L (2000) Cloning, overexpression and purification of novobiocic acic synthetase from Streptomyces spheroides NCIB 11891; J. Biol. Chem. 275, 21754-21760
Steffensky M, Mühlenweg A, Wang Z-X, Wemakor E, Li S-M, Heide L (2000) Molecular cloning of the novobiocin biosynthetic gene cluster of Streptomyces spheroides NCIB 11891. Antimicrob. Agents Chemother. 44, 1214-1222
Boehm R, Sommer S, Li S-M, Heide L (2000) Genetic engineering of shikonin biosynthesis: Expression of the bacterial ubiA gene in Lithospermum erythrorhizon. Plant & Cell Physiol. 41: 911-919
Boehm R, Sommer S, Severin K, Li S-M, Heide L (2000) Active expression of the ubiA Gene from E.coli in tobacco: Influence of plant ER-specific signal peptides on the expression of a membrane-bound prenyltransferase in plant cells. Transgenic Res. 9: 477-486
Yamamoto H, Inoue K, Li S-M, Heide L (2000) Geranylhydroquinone 3’’-hydroxylase, a cytochrome P-450 monooxygenase from Lithospermum erythrorhizon cell suspension cultures. Planta, 210:312-317
Schmid B, Lüdtke R, Selbmann H-K, Schaffner W, Kötter I, Tschirdewahn B, Heide L (2000) Wirksamkeit und Verträglichkeit eines standardisierten Weidenrindenextraktes bei Arthrose-Patienten: Randomisierte, placebo-kontrollierte Doppelblindstudie; Z. Rheumatol. 59: 314-320
Sommer S, Köhle A,Yazaki K, Shimomura K, Bechthold A, Heide L (1999) Genetic engineering of shikonin biosynthesis. Hairy root cultures of Lithospermum erythrorhizon transformed with the bacterial ubiC gene. Plant Mol. Biol. 39: 683-693
Mühlenweg A, Melzer M, Li S-M, Heide L (1998) 4-Hydroxybenzoate 3-geranyltransferase from Lithospermum erythrorhizon. Purification of a plant membrane-bound prenyltransferase. Planta 205: 407-413
Lange BM, Severin K, Bechthold A, Heide L (1998) Regulatory role of microsomal 3-hydroxy-3-methylglutaryl-coenzyme A reductase for shikonin biosynthesis in Lithospermum erythrorhizon cell suspension cultures. Planta 204: 234-241
Li S-M, Hennig S, Heide L (1998) Shikonin: a geranyl diphosphate-derived plant hemiterpenoid formed via the mevalonate pathway. Tetrahedron Letters 39: 2721-2724
Li S-M, Hennig S, Heide L (1998) Biosynthesis of the dimethylallyl moiety of novobiocin via a non-mevalonate pathway. Tetrahedron Letters 39: 2717-2720
Steffensky M, Li S-M, Vogler B, Heide L (1998) Novobiocin biosynthesis in Streptomyces spheroides: Identification of a dimethylallyl diphosphate : 4-hydroxyphenylpyruvate dimethylallyl transferase. FEMS Microbiol. Lett. 161: 69-74
Sommer S, Siebert M, Bechthold A, Heide L (1998) Specific induction of secondary product formation in transgenic plant cell cultures using an inducible promotor. Plant Cell Rep 17: 891-896
Li S-M, Bechthold A, Heide L (1998) Sequence announcement AF053721: Putative retrotransposon polyprotein from Lithospermum erythrorhizon. Plant Mol. Biol. 38: 505
Sommer S, Heide L (1998) Expression of bacterial chorismate pyruvate-lyase in tobacco: Evidence for the presence of chorismate in the plant cytosol. Plant & Cell Physiol. 39: 1240-1244
Li S-M, Wang Z-X, E. Wemakor, Heide L (1997) Metabolization of the artificial secondary metabolite 4-hydroxybenzoate in ubiC-transformed tobacco. Plant & Cell Physiology 38, 844-850
Brühl A, Schmid B, Feser H, Heide L (1997) Laienverständnis pflanzlicher und synthetischer Arzneimittel. Ergebnisse einer repräsentativen Befragung. Pharmazeut. Ztg. 31: 11-19
Yazaki K, Kataoka M, Honda G, Severin K, Heide L (1997) cDNA Cloning and gene expression of phenylalanin ammonia-lyase in Lithospermum erythrorhizon. Biosci. Biotech. Biochem. 61, 1995-2003
Boehm R, Li S-M, Melzer M, Heide L (1997) 4-Hydroxybenzoate prenyltransferases in cell-free extracts of Lithospermum erythrorhizon cell cultures. Phytochemistry 44, 419-424
Li S-M, Wang Z-X, Heide L (1997) Purification of UDP-glucose:4-hydroxybenzoate glucosyltransferase from cell cultures of Lithospermum erythrorhizon. Phytochemistry 46: 27-32
Wang Z-X, Li S-M, Löscher R, Heide L (1997) 4-Coumaroyl coenzyme A 3-hydroxylase activity from cell cultures of Lithospermum erythrorhizon and its relationship to polyphenol oxidase. Arch. Biochem. Biophys. 347: 249-255
Gaisser S, Heide L (1996) Inhibiton and regulation of shikonin biosynthesis in suspension cultures of Lithospermum. Phytochemistry 41, 1065-1072
Li S-M, Wang Z-X, Heide L (1996) Purification and characterization of an alcohol dehydrogenase from Lithospermum erythrorhizon cell cultures. Plant Cell Rep. 15, 786-790
Siebert M, Sommer S, Li S-M, Wang Z-X, Severin K, Heide L (1996) Genetic engineering of plant secondary metabolism. Accumuation of 4-hydroxybenzoate glucosides as a result of the expression of the bacterial ubiC gene in tobacco. Plant Physiol. 112, 811-819
Sommer S, Severin K, Camara B, Heide L (1995) Intracelluar localization of geranylpyrophosphate synthase from cell cultures of Lithospermum erythrorhizon. Phytochemistry 38, 623-627
Melzer M, Heide L (1994) Characterization of polyprenyldiphosphate:4-hydroxybenzoate polyprenyltransferase from Escherichia coli. Biochim. Biophys. Acta 1212, 93-102
Siebert M, Severin K, Heide L (1994) Formation of 4-hydroxybenzoate in Escherichia coli: characterization of the ubiC gene and its encoded enzyme chorismate pyruvate-lyase. Microbiology 140, 897-904
Löscher R, Heide L (1994) Biosynthesis of p-hydroxybenzoate from p-coumarate and p-coumaroyl-coenzyme A in cell-free extracts of Lithospermum erythrorhizon cell cultures. Plant Physiol. 106, 271-279
Schaaf PM, Heide L, Leistner E, Tani Y, El-Olemy MM (1993) Immobilization of isochorismate hydroxymutase. Comparison of native versus immobilized enzyme. J. Nat. Prod. 56: 1304-1312
Heide L, Melzer M, Siebert M, Bechthold A, Schröder J, Severin K (1993) Clarification of the Escherichia coli genetic map in the 92-minute region containing the ubiCA operon and the plsB, dgk, lexA, and dinF genes. J Bacteriol. 175: 5728-5729.
Cho H, Heide L, Floss HG (1992) Synthesis of D-(-)-[1,7-13C2]shikimic acid. J. Labell. Comp. Radiopharm. XXXI, 589-594
Siebert M, Bechthold A, Melzer M, May U, Berger U, Schröder G, Schröder J, Severin K, Heide L (1992) Ubiquinone biosynthesis. Cloning of the genes coding for chorismate pyruvate-lyase and 4-hydroxybenzoate octaprenyl transferase from Escherichia coli. FEBS Letters 307, 347-350
Bechthold A, Berger U, Heide L (1991) Parthial purification, properties and kinetic studies of UDP-Glucose:p‑hydroxybenzoate glucosyl transferase from cell cultures of Lithospermum erythrorhizon. Arch. Biochem. Biophys. 288, 39-47
Yazaki K, Heide L, Tabata M (1991) Formation of p-hydroxybenzoic acid from p-coumaric acid by cell free extracts of Lithospermum erythrorhizon cell cultures. Phytochemistry 30, 2233-2236
Heide L (1991) Enzymatic synthesis of labelled geraniol. J. Labell. Comp. Radiopharm. XXIX, 1261-1262
Heide L, Tabata M (1989) Facile enzymatic synthesis of labelled geranylpyrophosphate. Tetrahedron Letters 30, 813‑814
Heide L, Floss HG, Tabata M (1989) Incorporation of shikimic acid into p‑hydroxybenzoic acid in Lithospermum erythrorhizon cell cultures. Phytochemistry 28, 2643‑2645
Heide L, Nishioka N, Fukui H, Tabata M (1989) Enzymatic regulation of shikonin biosynthesis in Lithospermum erythrorhizon cell cultures. Phytochemistry 28, 1873‑1877
Heide L, Berger U (1989) Partial purification and properties of geranylpyrophosphate synthase from Lithospermum erythrorhizon cell cultures. Arch. Biochem. Biophys. 273, 331‑338
Heide L (1988) Geranylpyrophosphate synthase from cell cultures of Lithospermum erythrorhizon. FEBS Letters 237, 159‑162
Heide L, Tabata M (1987) Enzyme activities in cell‑free extracts of shikonin‑producing Lithospermum erythrorhizon cell suspension cultures. Phytochemistry 26, 1645‑1650
Heide L, Tabata M (1987) Geranylpyrophosphate: p‑hydroxybenzoate geranyltransferase activity in extracts of Lithospermum erythrorhizon cell cultures. Phytochemistry 26, 1651‑1655
Heide L, Leistner E (1983) Enzyme activities in extracts of anthraquinone‑containing cells of Galium mollugo L. Phytochemistry 22, 659‑662
Heide L, Leistner E (1982) Versuche zur Synthese natürlich vorkommender prenylierter Naphthalinderivate. Nachweis eines neuen Prenylnaphthochinonderivates in Galium mollugo. Z. Naturforsch. 37c, 354‑362
Heide L, Arendt S, Leistner E (1982) Enzymatic synthesis, characterization, and metabolism of the coenzyme A ester of o‑succinylbenzoic acid, an intermediate in menaquinone (vitamin K2) biosynthesis. J. Biol. Chem. 257, 7396‑7400
Heide L, Kolkmann R, Arendt S, Leistner E (1982) Enzymic synthesis of o‑succinylbenzoyl‑CoA in cell free extracts of anthraquinone producing Galium mollugo L. cell suspension cultures. Plant Cell Rep. 1, 180‑182
Heide L, Leistner E (1981) Enzymatic synthesis of the coenzyme A ester of o‑succinylbenzoic acid, an intermediate in menaquinone (vitamin K2) biosynthesis. FEBS Letters 128, 201‑204
Heide L, Leistner E (1981) 2‑Methoxycarbonyl‑3‑prenyl‑1,4‑ naphthoquinone, a metabolite related to the biosynthesis of mollugin and anthraquinones in Galium mollugo L. J. Chem. Soc. Chem. Comm. 1981, 334‑ 336