generation of transgenic cell and mouse models including Cre/lox-based genome engineering, real time imaging of biomolecules in live cells and tissues, cell tracking and fate mapping in mice (Feil)
lipid and membrane technologies including: preparation of model systems (small, large and giant unilamellar vesicles and supported lipid bilayers) and automated single vesicle analysis; recombinant protein expression, purification, and labeling; advanced microscopy setups including: laser scanning confocal microscopy with incubator for live cell imaging (Zeiss LSM 710 with Confocor 3 for fluorescence correlation spectoscopy), single molecule microscopy (via total internal reflectance, home-built), and atomic force microscopy (JPK NanoWizard II) (Garcia-Saez)
peptide synthesis, mass spectrometry, and flow cytometry (Kalbacher)
flow cytometry, high throughput real-time PCR, high resolution fluorescence microscopy / life-cell imaging (Schulze-Osthoff)
solid-phase peptide synthesis, protein semisynthesis (Schwarzer)
Protein-Structure-Pipeline including protein production in E.coli and cell culture, protein purification by HPLC chromatography (Äkta Prime, Purifier, Express), protein quality analysis using CD-spectroscopy, dynamic light scattering (DLS) and analytical size-exclusion chromatography (Äkta Ettan), protein crystallization using a liquid-handling-system (Freedom evo 150), structure determination at the home source system (MicroMAX 007-HF, MAR345 detector), and interaction analysis by SPR (Biacore 2000) (Stehle)
