Biochemistry
- Phage display
- Production and purification of recombinant proteins (Äkta pure system)
- Affinity determination (BLItz, Octet)
- Western blotting
- ELISA and bead-based ELISA (luminex)
- FACS scanning and sorting
- Immunoprecipitation and Co-immunoprecipitation
Molecular biology
- Cloning (classical, Gibson, mutagenesis)
- qRT-PCR
- Lenti virus generation and production (S2)
- CRISPR technology
Cell biology
- Cell line cultivation
- Generation and characterization of stable chromobody cell lines
- Lentiviral DNA transduction
- Intracellular binding studies using fluorescent-2-hybrid assay
- 3D cell culture
- Tumor cell migration and invasion studies
- Confocal and video microscope suitable for FRAP and FRET analyses (Zeiss LSM510 and cell observer)
- Fully automated high-throughput microscopy (ImageXpress, Molecular devices)
- High-content analysis
- Compound and siRNA screening
- Primary cell culture