Quantitative Proteomics

Proteome Center Tuebingen

Proteome Center Tuebingen (PCT) is a proteomics core facility of the University of Tuebingen and a member of the Quantitative Biology Center (www.qbic.uni-tuebingen.de). Members of the PCT team are:

  • Prof. Dr. Boris Macek (Director)
  • Dr. Mirita Franz-Wachtel (Core Facility Head)
  • Dr. Ana Velic
  • Irina Droste-Borel, Dipl. Biol.
  • Silke Wahl, Dipl. Biol.
  • Anke Biedermann, Dipl. Biol.

We offer various workflows for qualitative and quantitative analyses of proteins, proteomes and subproteomes (e.g. interactome, phosphoproteome, acetylome, ubiquitylome). Other posttranslational modifications can also be analyzed upon request.

Core Facility measurements are routinely performed on an Orbitrap Exploris 480 mass spectrometer and analyzed using MaxQuant (DDA) or Spectronaut (DIA) software. Quantitative workflows are mainly based on stable isotope labeling (SILAC, TMT, dimethyl labeling) or label-free quantification.

Please note that for a successful proteomics project:

  • Genome sequence or protein database of the organism of interest has to be available
  • Proteins should be delivered in µg amount as precipitated extracts or gel spots
  • Proteins/gels must NOT be radioactively labeled
  • Theoretical background of the project and protocols employed prior to MS measurement should be discussed with the PCT in detail in order to adapt the adequate analysis method

Our operational and access policies can be found here: Nutzer- und Gebührenordnung (German only).

For further inquiries, requests and price information please send an e-mail to pctspam prevention@ifiz.uni-tuebingen.de.

Privacy settings

Our website uses cookies. Some of them are mandatory, while others allow us to improve your user experience on our website. The settings you have made can be edited at any time.

or

Essential

in2code
Name

in2cookiemodal-selection

Use

Required to save the user selection of the cookie settings.

Lifetime

3 months

Name

be_lastLoginProvider

Use

Required for the TYPO3 backend login to determine the time of the last login.

Lifetime

3 months

Name

be_typo_user

Use

This cookie tells the website whether a visitor is logged into the TYPO3 backend and has the rights to manage it.

Lifetime

Browser session

Name

ROUTEID

Use

These cookies are set to always direct the user to the same server.

Lifetime

Browser session

Name

fe_typo_user

Use

Enables frontend login.

Lifetime

Browser session

Videos

in2code
Name

iframeswitch

Use

Used to show all third-party contents.

Lifetime

3 months

YouTube
Name

yt-player-bandaid-host

Use

Is used to display YouTube videos.

Lifetime

Persistent

Name

yt-player-bandwidth

Use

Is used to determine the optimal video quality based on the visitor's device and network settings.

Lifetime

Persistent

Name

yt-remote-connected-devices

Use

Saves the settings of the user's video player using embedded YouTube video.

Lifetime

Persistent

Name

yt-remote-device-id

Use

Saves the settings of the user's video player using embedded YouTube video.

Lifetime

Persistent

Name

yt-player-headers-readable

Use

Collects data about visitors' interaction with the site's video content - This data is used to make the site's video content more relevant to the visitor.

Lifetime

Persistent

Name

yt-player-volume

Use

Is used to save volume preferences for YouTube videos.

Lifetime

Persistent

Name

yt-player-quality

Use

Is used to save the quality settings for YouTube videos.

Lifetime

Persistent

Name

yt-remote-session-name

Use

Saves the settings of the user's video player using embedded YouTube video.

Lifetime

Browser session

Name

yt-remote-session-app

Use

Saves the settings of the user's video player using embedded YouTube video.

Lifetime

Browser session

Name

yt-remote-fast-check-period

Use

Saves the settings of the user's video player using embedded YouTube video.

Lifetime

Browser session

Name

yt-remote-cast-installed

Use

Saves the user settings when retrieving a YouTube video integrated on other web pages

Lifetime

Browser session

Name

yt-remote-cast-available

Use

Saves user settings when retrieving integrated YouTube videos.

Lifetime

Browser session

Google
Name

ANID

Use

Used for targeting purposes to profile the interests of website visitors in order to display relevant and personalized Google advertising.

Lifetime

2 years

Name

SNID

Use

Google Maps - Google uses these cookies to store user preferences and information when you view pages with Google Maps.

Lifetime

1 month

Name

SSID

Use

Used to store information about how you use the site and what advertisements you saw before visiting this site, and to customize advertising on Google resources by remembering your recent searches, your previous interactions with an advertiser's ads or search results, and your visits to an advertiser's site.

Lifetime

6 months

Name

1P_JAR

Use

This cookie is used to support Google's advertising services.

Lifetime

1 month

Name

SAPISID

Use

Used for targeting purposes to profile the interests of website visitors in order to display relevant and personalized Google advertising.

Lifetime

2 years

Name

APISID

Use

Used for targeting purposes to profile the interests of website visitors in order to display relevant and personalized Google advertising.

Lifetime

6 months

Name

HSID

Use

Includes encrypted entries of your Google account and last login time to protect against attacks and data theft from form entries.

Lifetime

2 years

Name

SID

Use

Used for security purposes to store digitally signed and encrypted records of a user's Google Account ID and last login time, enabling Google to authenticate users, prevent fraudulent use of login credentials, and protect user data from unauthorized parties. This may also be used for targeting purposes to display relevant and personalized advertising content.

Lifetime

6 months

Name

SIDCC

Use

This cookie stores information about user settings and information for Google Maps.

Lifetime

3 months

Name

NID

Use

The NID cookie contains a unique ID that Google uses to store your preferences and other information.

Lifetime

6 months

Name

CONSENT

Use

This cookie tracks how you use a website to show you advertisements that may be of interest to you.

Lifetime

18 years

Name

__Secure-3PAPISID

Use

This cookie is used to support Google's advertising services.

Lifetime

2 years

Name

__Secure-3PSID

Use

This cookie is used to support Google's advertising services.

Lifetime

6 months

Name

__Secure-3PSIDCC

Use

This cookie is used to support Google's advertising services.

Lifetime

6 months

Legal details Privacy policy