Transformation Unit Protocols
Cell Culture 01.doc
Cell Culture Maintenance (Arabidopsis Col-0)
BY-2-protopl.doc
BY-2 Tobacco Cell Suspension Protoplasts
(NOTE: we no longer maintain BY-2 suspension cells or culture since 2020).
PEG transfection protocol 2018
Arabodopsis Cell Culture Protoplasts
REFERENCING
Please cite for the standard protoplast transfection protocol after Feb. 2017:
Mehlhorn D.G., Wallmeroth N., Berendzen K.W., Grefen C. (2018) 2in1 Vectors Improve In Planta BiFC and FRET Analyses. In: Hawes C., Kriechbaumer V. (eds) The Plant Endoplasmic Reticulum. Methods in Molecular Biology, vol 1691. Humana Press, New York, NY.
Please cite for the 96-well transfection protocol:
Berendzen KW, Böhmer M, Wallmeroth N, Peter S, Vesić M, Zhou Y, Tiesler FK, Schleifenbaum F, Harter K. Screening for in planta protein-protein interactions combining bimolecular fluorescence complementation with flow cytometry. Plant Methods. 2012 Jul 12;8(1):25.
Transfections performed before Feb.2017 cite:
Schütze K, Harter K, Chaban C (2009) Bimolecular fluorescence complementation (BiFC) to study protein-protein interactions in living plant cells. Methods Mol Biol 479: 189-202.
STABLE TRANSFORMATIONS
PROTOCOLS
PotatoStabile.pdf
Stable Transformation protocol for potato
Transformation protocol for tobacco
Protocol for tomato stable transformation
Protocol for tomato stable transformation
LotusAgroTrafo1.pdf
Protocol for Lotus stable transformation
REFERENCING
Please cite for tomato transformations:
Wittmann et al. 2015. Plant Pathology. Doi: 10.1111/ppa.12417
http://onlinelibrary.wiley.com/doi/10.1111/ppa.12417/abstract
OTHER PROTOCOLS
Protoplasts isolation from leaves of Arabidopsis thaliana