more information

• Location
• Techniques provided
• Equipment
• Service provided
• List of LM stains
• Confocal Laser Scanning Microscopes

Location

Auf der Morgenstelle 32, floor 3

Techniques provided include:

• Negative Staining
• Glycerol Spraying
• Heavy Metal Coating/Carbon Coating
• Sputter Coating
• Freeze-Drying
• Freeze Fracture
• Critical-Point Drying
• Cryoimmobilisation (High Pressure Freezing (MPI für Entwicklungsbiologie), Propan Jet Freezing, Plunge Freezing)
• Freeze-Substitution
• Resin Embedding (Spurr, Epon)
• (Low Temperature) Embedding (LR White/Gold, Lowicryls HM20, K11M)
• Technovit Embedding
• Microwave fixation and microwave resin embedding
• Ultrathin Resin Sectioning
• Ultrathin Tokuyasu Cryosectioning for Immunolabelling
• Resin Section and Cryosection Immunofluorescence and Immunogold Labelling, Pre-embedding Immunogold Labelling
• Correlative light and electron microscopy CLEM
• Confocal Light Microscopy

Equipment:

• Transmission Electron Microscope JEOL 120 kV 1400plus with Tietz TemCam F-416 CMOS camera
• Epi-fluorescence microscope Zeiss Imager M2 with Hamamatsu ORCA-flash 4.0 V.2 CMOS camera and Axiocam 512 color
• Evaporation and Freeze Fracture Units Balzers 400T
• Critical-Point Dryer
• Sputter Coater
• Glow Discharge Unit
• Compact Coating Unit CCU-010
• Microwave Pelco Biowave Pro
• Freeze-Substitution and Low Temperature Embedding Unit Leica EMAFS and EMAFS2
• Reichert Ultracut E
• Ultramicrotome Leica UC7
• Cryo-Ultramicrotome Leica UC7/FC7
• Pyramitome LKB
• Confocal Microscope Leica TCS SP8 upright (Laser lines 405, 458, 476, 488, 496, 514, 561, 594, 633)
• Confocal Microscope Leica TCS SP8 (Laser lines: diode 405, pulsed 440, pulsed 470, Argon 488, 496, 514, DPSS 561, HeNe 594, HeNe 633) eqipped with resonant scanner, HyD detectors and FLIM & FCS
• Confocal Microscope Zeiss LSM 880 Airyscan
• Photo camera (high resolution) for plants, agar plates gels etc. with equipment, instruction by EM staff required

TEM Jeol 120 kV 1400plus

Epifluorescence microscope Zeiss Imager M.2

Pyramitome LKB, ultramicrotome Reichert Ultracut E and cryo-ultramicrotome Leica UCT

CLSM Leica SP8 AOBS

CLSM Leica SP8 AOBS with FLIM and FCS unit

Service provided:

• Introduction in use of equipment
• Assistance in preparation of samples
• Help with designing experiments
• Collaboration (for external users please contact Dr. York-Dieter Stierhof)
• Central storage of embedded samples and TEM grids
• Development, printing, digitization and archiving of negatives

The unit provides gold markers of different sizes and specificity, fluorescent markers, silver enhancers, fixatives, embedding resins (Spurr, Epon, LR White, LR Gold, Lowicryls HM20, K4M, K11M, Technovit), coated TEM grids, diamond knives for resin and cryosectioning, heavy metal stains, a number of light microscopic stains (see below) etc.

Confocal Laser Scanning Microscopes

a)

b)

c)

User Rules and Responsibilities:

1. Need to be introduced before using the CLSM.
2. Inform Natalie about any problems with the Leica machines and about problems with the Zeiss confocal.
3. Save temporary your data on volume D:/, and remove your data from the computer.

Service provided by Sandra (Leica SP8):

1. Contact with technical service of Leica.
2. Instruction in use and answer to the questions when it doesn't work.
3. For external users please contact Dr. Sandra Richter

Service provided by Sandra (Zeiss LSM880):

1. Contact with technical service of Zeiss.
2. Instruction in use and answer to the questions when it doesn't work.
3. For external users please contact Dr. Sandra Richter

List of LM Stains