Interfakultäres Institut für Mikrobiologie und Infektionsmedizin

List of Publications

Samuel Wagner Google Scholar link

 

Research Articles

  1. Grin, I., Maksymenko, K., Wörtwein, T., & ElGamacy, M. (2024). The Damietta Server: a comprehensive protein design toolkit. Nucleic acids research, 52(W1), W200–W206. https://doi.org/10.1093/nar/gkae297
  2. Moldt J.-A., Festl-Wietek T., Fuhl W., Zabel S., Claassen M., Wagner S., Nieselt K., Herrmann-Werner A., (2024) Assessing Artificial Intelligence Awareness and Identifying Essential Competencies: Insights from Key Stakeholders in Integrating Ai into Medical Education. Available at SSRN: SSRN Preprint
  3. Boudrioua A., Joiner J.D., Grin I., Kronenberger T., Korotkov V.S., Steinchen W., Kohler A., Schminke S., Schulte J., Pietsch M., Naini A., Kalverkamp S, Hotop S., Coyle T., Piselli C, Coles M., Rox K., Marschal M., Bange G., Flieger A., Poso A., Bronstrup M., Hartmann M.D., Wagner S. (2024) Discovery of a synthetic small molecule targeting the central regulator of Salmonella pathogenicity. bioRxiv Preprint
  4. Malmsheimer S., Grin I., Bohn E., Franz-Wachtel M., Macek B., Sahr T., Smollich F., Chetrit D., Meir A., Roy C., Buchrieser C., Wagner S. (2024) The T4bSS of Legionella features a two-step secretion pathway with an inner membrane intermediate for secretion of transmembrane effectors. bioRxiv Preprint
  5. Joiner J.D., Steinchen W., Mozer N., Kronenberger T., Bange G., Poso A., Wagner S., Hartmann M.D. (2023) HilE represses the activity of the Salmonella virulence regulator HilD via a mechanism distinct from that of intestinal long-chain fatty acids. Journal of Biological Chemistry
  6. Fromm K., Boegli A., Ortelli M., Wagner A., Bohn E., Malmsheimer S., Wagner S., Dehio C. (2022) Bartonella taylorii: A model organism for studying Bartonella infection in vitro and in vivo.  Frontiers in Microbiology
  7. Singh N., Kronenberger T., Eipper A.,  Weichel F., Franz M., Macek B., Wagner S. (2021). Conserved salt bridges facilitate assembly of the helical core export apparatus of a Salmonella enterica type III secretion system.  Journal of Molecular Biology
  8. Kuhlen L., Johnson S., Zeitler A., Bäurle S., Deme J.C., Ceasar J.J.E., Debo R., Fisher J., Wagner S., Lea S.M. (2020). The substrate specificity switch FlhB assembles onto the export gate to regulate type three secretion. Nat. Commun. 11:1296.
  9. Westerhausen S., Nowak M., Torres-Vargas C.E., Bilitewski U., Bohn E., Grin I., Wagner S. (2020). A NanoLuc luciferase-based assay enabling the real-time analysis of protein secretion and injection by bacterial type III secretion systems. Mol. Microbiol. doi: 10.1111/mmi.14490
  10. Torres-Vargas C.E., Kronenberger T., Roos N., Dietsche T., Poso A., Wagner S. (2019). The inner rod of virulence-associated type III secretion systems constitutes a needle adapter of one helical turn that is deeply integrated into the system's export apparatus. Mol. Microbiol. 112:918-931.
  11. Natarajan J., Moitra A., Zabel S., Singh N., Wagner S., Rapaport D. (2019).  Yeast can express and assemble bacterial secretins in the mitochondrial outer membrane. Microb Cell. 7(1):15-27
  12. Steimle A., Menz S., Bender A., Ball B., Weber A.N.R., Hagemann T., Lange A., Maerz J.K., Parusel R., Michaelis L., Schäfer A., Yao H., Löw H.C., Beier S., Tesfazgi Mebrhatu M., Gronbach K., Wagner S., Voehringer D., Schaller M., Fehrenbacher B., Autenrieth I.B., Oelschlaeger T.A., Frick J.S. (2019). Flagellin hypervariable region determines symbiotic properties of commensal Escherichia coli strains. PLoS Biol. 17:e3000334.
  13. Steenhuis M., Abdallah A.M., de Munnik S.M., Kuhne S., Sterk G.J., van den Berg van Saparoea B., Westerhausen S., Wagner S., van der Wel N.N., Wijtmans M., van Ulsen P., Jong W.S.P., Luirink J. (2019). Inhibition of autotransporter biogenesis by small molecules. Mol. Microbiol. 12:300.
  14. Krampen L., Malmsheimer S., Grin I., Trunk T., Lührmann A., de Gier J.W., Wagner S. (2018). Revealing the mechanism of membrane protein export by virulence-associated bacterial secretion systems. Nat. Commun. 9:3467. Link.
  15. Gratani F.L., Horvatek P., Geiger T., Borisova M., Mayer C., Grin I., Wagner S., Steinchen W., Bange G., Velic A., Macek B., Wolz C. (2018). Regulation of the opposing (p)ppGpp synthetase and hydrolase activities in a bifunctional RelA/SpoT homologue from Staphylococcus aureus. PLoS Genet. 14:e1007514. Link.
  16. Kuhlen L., Abrusci P., Johnson S., Gault J., Deme J., Caesar J., Dietsche T., Tesfazgi Mebrhatu M., Ganief T., Macek B., Wagner S., Robinson C.V., Lea S.M. (2018). Structure of the core of the type three secretion system export apparatus. Nat. Struct. Mol. Biol. 25:583-90. Link.
  17. Fukumura T., Makino F., Dietsche T., Kinoshita M., Kato T., Wagner S., Namba K., Imada K., Minamino T. (2017). Assembly and stoichiometry of the core structure of the bacterial flagella type III export gate complex. PLoS Biology 15:e2002281. Link.
  18. Fabiani F., Renault T.T., Peters B., Dietsche T., Galvez E.J.C., Guse A., Freier K., Charpentier E., Strowig T., Franz-Wachtel M., Macek B., Wagner S., Hensel M., Erhardt M. (2017). A flagellum-specific chaperone facilitates assembly of the core type III export apparatus of the bacterial flagellum. PLoS Biology 15:2002267. Link.
  19. Baumgarten T., Schlegel S., Wagner S., Löw M., Eriksson J., Bonde I., Herrgård M.J., Heipieper H.J., Nørholm M., Slotboom D., de Gier J.W. (2017). Isolation and characterization of the E. coli membrane protein production strain Mutant56(DE3). Sci. Rep. 7:45089. Link.
  20. Dietsche T., Tesfazgi Mebrhatu M., Brunner M.J., Abrusci P., Yan J., Franz-Wachtel M., Schärfe C., Zilkenat S., Grin I., Galán J.E., Kohlbacher O., Lea S., Macek B., Marlovits T.C., Robinson C.V., Wagner S. (2016). Structural and functional characterization of the bacterial type III secretion export apparatus. PLoS Pathogens 12:e1006071. Link.
  21. Drechsler-Hake D., Alamir H., Günter M., Wagner S., Schütz M., Bohn E., Schenke-Layland K,, Pisano F., Dersch P., Autenrieth I.B., Autenrieth S.E. (2016). Mononuclear phagocytes contribute to intestinal invasion and dissemination of Yersinia enterocolitica. Int. J. Med. Microbiol. 306:357-66
  22. Zilkenat S., Franz-Wachtel M., Stierhof Y.-D., Galán J.E., Macek B., Wagner S. (2016). Determination of the stoichiometry of the complete bacterial type III secretion needle complex using a combined quantitative proteomic approach. Mol. Cell. Proteomics 15:1598-1609. Illustrated abstract.
  23. Monjarás Feria J.V., Lefebre M.D., Stierhof Y.-D., Galán J.E., Wagner S. (2015). Role of autocleavage in the function of a type III secretion specificity switch protein in Salmonella enterica serovar Typhimurium. mBio 6:e01459–15
  24. Ernst C.M., Kuhn S., Heilbronner S., Slavetinsky C.J., Gekeler C., Kraus D., Krismer B., Wagner S., Peschel A. (2015). The lipid-modifying multiple peptide resistance factor is an oligomer consisting of distinct interacting synthase and flippase subunits. mBio 6:e02340-14
  25. Fischer M., Zilkenat S., Gerlach R., Wagner S., Renard B. (2014). Pre‐ and postprocessing workflow for affinity purification mass spectrometry data. J. Proteome Res. 13:2239-49
  26. Grin I., Hartmann M.D., Sauer G., Hernandez Alvarez B., Schütz M., Wagner S., Madlung J., Macek B., Felipe-Lopez A., Hensel M., Lupas A., Linke D. (2014). A trimeric lipoprotein assists in trimeric autotransporter biogenesis in enterobacteria. J. Biol. Chem. 289:7388-98
  27. Jong W.S.P., Soprova Z., de Punder K., ten Hagen-Jongman C.M., Wagner S., Wickström D., de Gier J.W., Andersen P., van der Wel N.N., Luirink J. (2012). A structurally informed autotransporter platform for the efficient secretion and display of heterologous proteins. Microb. Cell Fact. 11:85
  28. Wickström D., Wagner S., Simonsson P., Pop O., Baars L., Ytterberg A.J., van Wijk K.J., Luirink J., de Gier J.W. (2011). Characterization of the consequences of YidC depletion on the inner membrane proteome of Escherichia coli using 2D blue native/ SDS-PAGE. J. Mol. Biol. 409:124-35
  29. Lara-Tejero M., Kato J., Wagner S., Liu X., Galán J.E. (2011). A sorting platform determines the order of protein secretion in bacterial type III systems. Science 331:1188-91
  30. Wickström D., Wagner S., Baars L., Ytterberg A.J., Klepsch M., van Wijk K.J., Luirink J., de Gier J.W. (2011). Consequences of depletion of the signal recognition particle in Escherichia coli. J. Biol. Chem. 286:4598-609
  31. Wagner S., Königsmeier L., Lara-Tejero M., Lefebre M., Marlovits T.C., Galán J.E. (2010). Organization and coordinated assembly of the type III secretion export apparatus. Proc. Natl. Acad. Sci. USA. 107:17745-50. Illustrated abstract.
  32. Wagner S., Klepsch M.M., Schlegel S., Appel A., Draheim R., Tarry M., Högbom M., van Wijk K.J., Slotboom D.J., Persson J.O., de Gier J.W. (2008). Tuning membrane protein overexpression in Escherichia coli. Proc. Natl. Acad. Sci. USA. 105:14371-6
  33. Klepsch M.M., Schlegel S., Wickström D., Friso G., van Wijk K.J., Persson J.O., de Gier J.W., Wagner S. (2008). Immobilization of the first dimension in 2D blue native/SDS-PAGE allows the relative quantification of membrane proteomes. Methods 46:48-53
  34. Wagner S., Pop O., Haan G.T., Baars L., Klepsch M., Genevaux P., Luirink J., de Gier J.W. (2008). Biogenesis of MalF and the MalFGK2 maltose transport complex in Escherichia coli requires YidC. J. Biol. Chem. 283:17881-90
  35. Baars L., Wagner S., Wickström D., Klepsch M.M., Ytterberg A.J., van Wijk K.J., de Gier J.W. (2008). Effects of SecE depletion on the inner and outer membrane proteomes of Escherichia coli. J. Bacteriol. 90:3505-25
  36. Wagner S., Baars L., Ytterberg A.J., Klussmeier A., Wagner C.S., Nord O., Nygren P.Å., van Wijk K.J., de Gier J.W. (2007). Consequences of membrane protein overexpression in Escherichia coli. Mol. Cell. Proteomics 6:1527-50
  37. Baars L., Ytterberg A.J., Drew D., Wagner S., Thilo C., van Wijk K.J., de Gier J.W. (2006). Defining the role of the Escherichia coli chaperone SecB using comparative proteomics. J. Biol. Chem. 281:10024-34
  38. Marani P., Wagner S., Baars L., Genevaux P., de Gier J.W., Nilsson I., Casadio R., von Heijne G. (2006). New Escherichia coli outer membrane proteins identified through prediction and experimental verification. Protein Sci. 15:884-9

Reviews

  1. Pais S., Kim E., Wagner S. (2023) Virulence-associated type III secretion systems in Gram-negative bacteria. Microbiology (Reading).doi: http://doi.org/10.1099/mic.0.001328
  2. Singh N., Wagner, S. (2019). Investigating the assembly of the bacterial type III secretion system injectisome by in vivo photocrosslinking. Int. J. Med. Microbiol. http://doi.org/10.1016/j.ijmm.2019.151331
  3. Natarajan J., Singh N., Rapaport D. (2019). Assembly and targeting of secretins in the bacterial outer membrane. Int. J. Med. Microbiol. http://doi.org/10.1016/j.ijmm.2019.06.002
  4. Stegmann E., Wagner S., Schwarz S. (2019). SFB 766: 12 years of research on the bacterial cell envelope. Int J Med Microbiol. 309(8):151360
  5. Wagner S., Grin I., Malmsheimer S., Singh N., Torres-Vargas C.E., Westerhausen S. (2018). Bacterial type III secretion sytems: A complex device for delivery of bacterial effector proteins into eukaryotic host cells. FEMS Microbiol. Lett. doi:10.1093/femsle/fny201. Link.
  6. Zilkenat S., Grin I., Wagner S. (2017). Stoichiometry determination of macromolecular membrane protein complexes. Biol. Chem. 398:155-64
  7. Galán J.E., Lara-Tejero M., Marlovits T., Wagner S. (2014). Bacterial type III secretion systems: Specialized nanomachines for protein delivery into target cells. Annu. Rev. Microbiol. 68:415–38
  8. Diepold A., Wagner S. (2014). Assembly of the bacterial type III secretion machinery. FEMS Microbiol. Rev. 38:802-22
  9. Luirink J., Zu Y., Wagner S., de Gier J.W. (2012). Biogenesis of inner membrane proteins in Escherichia coli. Biochim. Biophys. Acta 1817:965-76
  10. Wagner S., Lerch M., Drew D., de Gier J.W. (2006). Rationalizing membrane protein overexpression. Trends Biotechnol. 24:364-71

Book chapters

  1. Pais SV, Westerhausen S, Bohn E, Wagner S (2022). Analysis of SPI-1 Dependent Type III Secretion and Injection Using a NanoLuc Luciferase-Based Assay.  Methods Mol. Biol. 2427:57-71, 427:1-10.
  2. Wagner S, Diepold A. A Unified Nomenclature for Injectisome-Type Type III Secretion Systems (2020). Curr Top Microbiol Immunol.
  3. Zilkenat S., Dietsche T., Monjarás Feria J.V., Torres-Vargas C.E., Tesfazgi Mebrhatu M., Wagner S. Blue native PAGE analysis of bacterial secretion complexes. Methods Mol. Biol. 1615:321-351
  4. Schlegel S., Klepsch M., Wickström D., Wagner S., de Gier J.W. (2010). Comparative analysis of cytoplasmic membrane proteomes of Escherichia coli using 2D blue native/SDS-PAGE. Methods Mol. Biol. 619:257-69

Patents

  1. Wagner S., de Gier J.W. Expression system for proteins. EP 2268818