Proteome Center Tuebingen

Proteome Center Tuebingen (PCT) is a proteomics core facility of the Faculty of Science, University of Tuebingen and a member of the Quantitative Biology Center (www.qbic.uni-tuebingen.de). Since its establishment in 2003, the core facility has processed over 1,250 projects and contributed to over 280 research papers. Members of the PCT team are:

• Prof. Dr. Boris Macek (Director)
• Dr. Mirita Franz-Wachtel (Core Facility Head)
• Dr. Ana Velic
• Irina Droste-Borel, Dipl. Biol.
• Silke Wahl, Dipl. Biol.
• Anke Biedermann, Dipl. Biol.

We offer various workflows for qualitative and quantitative analyses of proteins, proteomes and subproteomes (e.g. interactome, phosphoproteome, acetylome, ubiquitylome). Other posttranslational modifications can also be analyzed upon request.

Core Facility measurements are routinely performed on an Orbitrap Exploris 480 mass spectrometer and analyzed using MaxQuant (DDA) or Spectronaut (DIA) software. Quantitative workflows are mainly based on stable isotope labeling (SILAC, TMT, dimethyl labeling) or label-free quantification.

Please note that for a successful proteomics project:

• Genome sequence or protein database of the organism of interest has to be available
• Proteins should be delivered in µg amount as precipitated extracts or gel spots
• Proteins/gels must NOT be radioactively labeled
• Theoretical background of the project and protocols employed prior to MS measurement should be discussed with the PCT in detail in order to adapt the adequate analysis method

Our operational and access policies can be found here: Nutzer- und Gebührenordnung (German only).

For further inquiries, requests and price information please send an e-mail to pctspam prevention@ifiz.uni-tuebingen.de.