Antibodies directed against the CD3e chain of the TCR/CD3 complex represent a strong T cell stimulus. Contact of T cells with anti-CD3-functionalized surfaces leads to a rapid spreading of cells on the surface within 2-5 minutes.
With RIfS it was possible to detect the stimulus dependent spreading of the T-cell line Jurkat label free and time resolved in flow system. Anti-CD3 antibodies were immobilised onto the RIfS transducers and the spreading of the jurkat cell line as well as the signal reduction of the lck-deficient cell line JCaM 1.6, the use of the spreading inhibitor cytochalsine D and Jurkat cell on different surfaces could be detected.
