We aim to develop collagen–hydroxyapatite (CoHA) composite biomaterials for applications in bone tissue engineering. Our approach involves using different types of collagen—specifically atelocollagen and telocollagen—as well as hydroxyapatite (HA) particles of varying sizes. Eventually, we will incorporate bioactive mimetic peptides into the constructs. In the final stage, stem cells will be introduced to colonize the scaffolds. The incorporation of each component induces structural and configurational changes within the 3D construct. We plan to monitor these changes using advanced, label-free technologies. Through this, we seek to gain deeper insights into the structural evolution of engineered biocompatible materials, as well as the dynamic interactions between living cells and non-living scaffolds.

In this project, samples prepared by the research laboratory led by Prof. Alexander-Friedrich (Klinik und Poliklinik für Mund-, Kiefer- und Gesichtschirurgie, Universitätsklinikum Tübingen) will be analyzed using our state-of-the-art multiphoton optical microscope. Collagen fiber morphology will be visualized through second harmonic generation imaging, while the distribution and presence of hydroxyapatite will be assessed via multiphoton fluorescence intensity and Raman spectral fingerprints. Furthermore, we will characterize the three-dimensional composition of the constructs using fluorescence lifetime imaging and phase plot analysis. This step-by-step imaging and analysis approach will enable us to uncover the structural transformations of the constituent building blocks in engineered bone-like materials. 

For more details, please contact Prof. Dai Zhang (dai.zhang-at-uni-tuebingen.de; Tel.: 070712977639)