Infection diseases caused by antibiotic resistant bacteria, especially MRSA (methicillin resistant staphylococcus aureus), remain a rising concern. Thiazolidine containing cyclopeptide Lugdunin, which was discovered in 2016, shows a promising antibiotic activity against MRSA. The structure was elucidated via NMR spectroscopy and mass spectrometry. In cooperation with our partners in Microbiology Tübingen, biosynthesis was resolved. A sythesis strategy via SPPS (solid phase peptide synthesis) was established, leading the way to broad research on biological activity.