Center for Plant Molecular Biology

Research Group Oecking

Function of plant 14-3-3 proteins


Reuter, L., Schmidt, T., Manishankar, P., Throm, C., Keicher, J., Bock, A., Droste-Borel, I. and Oecking, C., (2021). Light-triggered and phosphorylation-dependent 14-3-3 association with NON-PHOTOTROPIC HYPOCOTYL 3 is required for hypocotyl phototropism. Nature Communications, 12(1), pp.1-15.

Lenarčič T, Albert I, Böhm H, Hodnik V, Pirc K, Zavec AB, Podobnik M, Pahovnik D, Žagar E, Pruitt R, Greimel P, Yamaji-Hasegawa A, Kobayashi T, Zienkiewicz A, Gömann J, Mortimer JC, Fang L, Mamode-Cassim A, Deleu M, Lins L, Oecking C, Feussner I, Mongrand S, Anderluh G, Nürnberger T (2017) Eudicot plant-specific sphingolipids determine host selectivity of microbial NLP cytolysins. Science, 358, 1431 -1434.

Keicher J, Jaspert N, Weckermann K, Möller, C, Throm C, Kintzi A, Oecking C (2017) Arabidopsis 14-3-3 epsilon members contribute to polarity of PIN auxin carrier and auxin transport-related development. eLife 6:e24336.

Pacheco-Villalobos D, Díaz-Moreno S-M, van der Schuren A, Tamaki T, Kang JH, Gujas B, Novak O, Jaspert N, Li Z, Wolf S, Oecking C, Ljung K, Bulone V & Hardtke CS (2016) Assessment of High Auxin Steady State in Brachypodium Root Cell Elongation. Plant Cell 28, 1009-1024.

Gao J, van Kleeff PJM, Oecking C, Li KW, Erban, A, Kopka J, Hincha D, de Boer AH (2014) Light modulated activity of root alkaline/neutral invertase involves the interaction with 14-3-3 proteins. Plant J 80, 785-796.

van Kleeff PJM, Jaspert N, Li KW, Rauch S, Oecking C, de Boer AH (2014) Higher order Arabidopsis 14-3-3 mutants show 14-3-3 involvement in primary root growth both under control and abiotic stress conditions. J Experimental Botany 65, 5877-5888.

Stührwohldt N, Hartmann J, Dahlke RI, Oecking C, Sauter M (2014) The PSI family of nuclear proteins is required for growth in Arabidopsis. Plant Mol Biol 86, 289-302.

Jaspert N, Throm C, Oecking C (2011) Arabidopsis 14-3-3 proteins: fascinating and less fascinating aspects. Front Plant Sci 2:96, doi: 10.3389/fpls.2011.00096.

Caesar K, Elgass K, Chen Z, Huppenberger P, Witthöft J, Schleifenbaum F, Blatt MR, Oecking C, Harter K (2011) A fast brassinolide-regulated response pathway in the plasma membrane of Arabidopsis thaliana. Plant J 66, 528-540.

Witthöft J, Caesar K, Elgass K, Huppenberger P, Kilian J, Schleifenbaum F, Oecking C, Harter K (2011) The activation of the Arabidopsis P-ATPase 1 by the brassinosteroid receptor BRI1 is independent of threonine 948 phosphorylation. Plant Signaling & Behavior 6, 1063-1066.

Speth C, Jaspert N, Marcon C, Oecking C (2010) Regulation of the plant plasma membrane H+-ATPase by its C-terminal domain: what do we know for sure? Eur J Cell Biol 89, 145-151.

Li M, Doll J, Weckermann K, Oecking C, Berendzen KW, Schöffl F (2010) Detection of in vivo interactions between Arabidopsis class A-HSFs, using a novel BiFC fragment, and identification of novel class B-HSF interacting proteins. Eur J Cell Biol 89, 126-132.

Oecking C, Jaspert N (2009) Plant 14-3-3 proteins catch up with their mammalian orthologs. Curr Opin Plant Biol 12, 760-765.

Küfner I, Ottmann C, Oecking C, Nürnberger T (2009) Cytolytic toxins as triggers of plant immune response. Plant Signaling & Behavior 10, 977-979.

Ottmann C, Luberacki B, Küfner I, Koch W, Brunner F, Weyand M, Mattinen L, Pirhonen M, Anderluh G, Seitz H U, Nürnberger T, Oecking C (2009) A common toxin fold mediates microbial attack and plant defense. Proc Natl Acad Sci 106, 10359-10364.

Ottmann C, Weyand M, Sassa T, Inoue T, Kato N, Wittinghofer A, Oecking C (2009) A structural rationale for selective stabilization of anti-tumor interactions of 14-3-3 proteins by cotylenin A. J Mol Biol 386, 913-919.

Luberacki B, Weyand M, Seitz U, Koch W, Oecking C, Ottmann C (2008) Purification, crystallization and preliminary X-ray diffraction analysis of an oomycete-derived Nep1-like protein. Acta Cryst. F64, 1178-1180.

Ottmann C, Marco S, Jaspert N, Marcon C, Schauer, N, Vandermeeren C, Boutry M, Wittinghofer A, Rigaud J-L, Oecking C (2007) Structure of a 14-3-3 coordinated hexamer of the plant plasma membrane H+-ATPase by combining x-ray crystallo-graphy and electron cryomicroscopy. Mol Cell 25, 427-440.

Walter M, Chaban C, Schütze K, Batistic O, Weckermann K, Näke C, Blazevic D, Grefen C, Schumacher K, Oecking C, Harter K, Kudla J (2004) Visualisation of protein interactions in living plant cells using bimolecular fluorescence complementation. Plant J 40, 428-438.

Piotrowski M, Schemenewitz A, Lopoukhina A, Müller A, Janowitz T, Weiler EW, Oecking C (2004) Desulfo-Glucosinolate sulfotransferases from Arabidopsis thaliana catalyzing the final step in the biosynthesis of the glucosinolate core structure. J Biol Chem 279, 50717-50725.

Würtele M, Jelich-Ottmann C, Wittinghofer A, Oecking C (2003) Structural view of a fungal toxin acting on a 14-3-3 regulatory complex. EMBO J 22, 987-994.

Jaspert N, Oecking C (2002) Regulatory 14-3-3 proteins bind the atypical motif within the C-terminus of the plant plasma membrane H+-ATPase via their typical amphipathic groove. Planta 216, 136-139.

Rutschmann F, Stalder U, Piotrowski M, Oecking C, Schaller A (2002) LeCPK1, a calcium-dependent protein kinase from tomato. Plasma membrane targeting and biochemical characterization. Plant Physiol 129, 156-168.

Oecking C (2002) Die Protonenpumpe der pflanzlichen Zellmembran. BioSpektrum 1/2002, 27-30.

Besl H, Licht W, Oecking C, Schneider H, Nick P, Wagenitz G (2002) Strasburger-CD-ROM. Interaktive Studienhilfe und Wörterbuch zur 35. Auflage des Strasburger, Lehrbuch der Botanik. ISBN 3-8274-1070-3, Spektrum-Verlag.

Jelich-Ottmann C, Weiler EW, Oecking C (2001) Binding of regulatory 14-3-3 proteins to the C-terminus of the plant plasma membrane H+-ATPase involves part of its autoinhibitory region. J Biol Chem 276, 39852-39857.

Maudoux O, Batoko H, Oecking C, Gevaert K, Vandekerckhove J, Boutry M, Morsomme P (2000) A plant plasma membrane H+-ATPase expressed in yeast is activated by phosphorylation at its penultimate residue and binding of 14-3-3 regulatory proteins in the absence of fusicoccin. J Biol Chem 275, 17762-17770.

Oecking C, Hagemann K (1999) Association of 14-3-3 proteins with the C-terminal auto-

inhibitory domain of the plant plasma-membrane H+-ATPase generates a fusicoccin-binding complex. Planta 207, 480-482.

Schaller A, Oecking C (1999) Modulation of plasma membrane H+-ATPase activity differentially activates wound and pathogen defence responses in tomato plants. Plant Cell 11, 263-272.

Krekel F, Oecking C, Amrhein N, Macheroux P (1999) Substrate and inhibitor-induced

conformational changes in the structurally related enzymes UDP-N-acetyl-glucosamine-enolpyruvyl transferase (MurA) and 5-enol-pyruvylshikimate 3-phosphate synthase (EPSPS). Biochemistry 38, 8864-8878.

Svennelid F, Olsson A, Piotrowski M, Rosenquist M, Ottmann C, Larsson C, Oecking C, Sommarin M (1999) Phosphorylation of Thr-948 at the C terminus of the plasma membrane H+-ATPase creates a binding site for regulatory 14-3-3 protein. Plant Cell 11, 2379-2391.

Piotrowski M, Morsomme P, Boutry M, Oecking C (1998) Complementation of the

Saccharomyces cerevisiae plasma membrane H+-ATPase by a plant H+-ATPase generates a highly abundant fusicoccin binding site. J Biol Chem 273,

Piotrowski M, Oecking C (1998) Five new 14-3-3 isoforms from Nicotiana tabacum L.: implications for the phylogeny of plant 14-3-3 proteins. Planta 204, 127-130.

Oecking C, Piotrowski M, Hagemeier J, Hagemann K (1997) Topology and target

interaction of the fusicoccin-binding 14-3-3 homologs of Commelina communis. Plant J 12, 441-453.

Oecking C, Eckerskorn C, Weiler EW (1994) The fusicoccin receptor of plants is a

member of the 14-3-3 superfamily of eukaryotic proteins. FEBS Lett 352, 163-166.

Oecking C, Weiler EW (1991) Characterization and purification of the fusicoccin-binding

complex of Commelina communis. Eur J Biochem 199, 685-689.

Weiler EW, Meyer C, Oecking C, Feyerabend M, Mithöfer A (1990) The fusicoccin

receptor of higher plants. In: Plant gene transfer. C. Lamb, R. Beachy, eds., Alan R. Liss, New York, 153-164.


Würtele M, Jelich-Ottmann C, Oecking C, Wittinghofer A
Crystal structure of the ternary complex of 14-3-3, fusicoccin and the plasma membrane ATPase and methods for designing new herbicides.
US Patent 7521538 (2009)

Ottmann C, Weyand M, Rose R, Wolf A, Oecking C, Wittinghofer A (2006) Crystal structure of a complex between a 14-3-3 protein, a peptide ligand and fusicoccin and methods for the development of 14-3-3 protein client agonists. Deutsche Patentanmeldung

Würtele M, Jelich-Ottmann C, Oecking C, Wittinghofer A (2003) Crystal structure of the ternary complex of 14-3-3, fusicoccin and the plasma membrane ATPase and methods for designing new herbicides. EP2004001931