Publications

Department Microbial Bioactive Compounds

Voitsekhovskaia I, Ho YTC, Klatt C, Müller A, Machell DL, Tan YJ, Triesman M, Bingel M, Schittenhelm RB, Tailhades J, Kulik A, Maier ME, Otting G, Wohlleben W, Schneider T, Cryle M, Stegmann E. Altering glycopeptide antibiotic biosynthesis through mutasynthesis allows incorporation of fluorinated phenylglycine residues. (2024) RSC Chem Biol. doi: 10.1039/d4cb00140k. ⇓

Werner J, Umstätter F, Böhmann MB, Müller H, Beijer B, Hertlein T, Kaschnitz L, Bram V, Kleist C, Klika KD, Mühlberg E, Braune G, Wohlfart S, Gärtner M, Peter S, Zimmermann S, Haberkorn U, Ohlsen K, Brötz-Oesterhelt H, Mier W, Uhl P. Conjugation of Polycationic Peptides Extends the Efficacy Spectrum of β-Lactam Antibiotics. Adv Sci (Weinh). 2024 Nov 5:e2411406. doi: 10.1002/advs.202411406. ⇓

Peslalz P, Vorbach A, Bleisch A, Liberini E, Kraus F, Izzo F, Brötz-Oesterhelt H, Götz F, Plietker B. Chemical Predictive Modelling and Natural Product-based Divergent Synthesis - Design of Type B PPAPs with Nanomolar Activities against MRSA. Chemistry. 2024 Aug 19;30(46):e202401955. doi: 10.1002/chem.202401955. Epub 2024 Jul 29. ⇓

Leeten K, Jacques N, Esquembre LA, Schneider DC, Straetener J, Henriksen C, Musumeci L, Putters F, Melo S, Sánchez-López E, Giera M, Penoy N, Piel G, Verlaine O, Amoroso A, Joris B, Slavetinsky CJ, Goffin E, Pirotte B, Frees D, Brötz-Oesterhelt H, Lancellotti P, Oury C. Ticagrelor alters the membrane of Staphylococcus aureus and enhances the activity of vancomycin and daptomycin without eliciting cross-resistance. mBio. 2024 Sep 23:e0132224. doi: 10.1128/mbio.01322-24. ⇓

Ludwig KC, Puls JS, Matos de Opitz CL, Innocenti P, Daniel JM, Bornikoel J, Arts M, Krannich S, Straetener J, Brajtenbach D, Henrichfreise B, Sass P, Mueller A, Martin NI, Brötz-Oesterhelt H, Kubitscheck U, Grein F, Schneider T. The Dual Mode of Antibacterial Action of the Synthetic Small Molecule DCAP Involves Lipid II Binding. J Am Chem Soc. 2024 Sep 11;146(36):24855-24862. doi: 10.1021/jacs.4c05138. Epub 2024 Aug 28. ⇓

Ammanath AV, Matsuo M, Wang H, Kraus F, Bleisch A, Peslalz P, Mohammad M, Deshmukh M, Grießhammer A, Purkayastha M, Vorbach A, Macek B, Brötz-Oesterhelt H, Maier L, Kretschmer D, Peschel A, Jin T, Plietker B, Götz F. Antimicrobial Evaluation of Two Polycyclic Polyprenylated Acylphloroglucinol Compounds: PPAP23 and PPAP53. Int J Mol Sci. 2024 Jul 23;25(15):8023. doi: 10.3390/ijms25158023. ⇓

Berscheid A, Straetener J, Schilling NA, Ruppelt D, Konnerth MC, Schittek B, Krismer B, Peschel A, Steinem C, Grond S, Brötz-Oesterhelt H. The microbiome-derived antibacterial lugdunin acts as a cation ionophore in synergy with host peptides. mBio. 2024 Aug 12:e0057824. doi: 10.1128/mbio.00578-24. ⇓ 

Maier L, Stein-Thoeringer C, Ley R, Brötz-Oesterhelt H, Link H, Ziemert N, Wagner S, Peschel A. Integrating research on bacterial pathogens and commensals to fight infections - an ecological perspective. The Lancet Microbe. (2024) doi: 10.1016/S2666-5247(24)00049-1. ⇓

Nouioui I, Zimmermann A, Hennrich O, Xia S, Rössler O, Makitrynskyy R, Gomez-Escribano JP, Pötter G, Jando M, Döppner M, Wolf J, Neumann-Schaal M, Hughes CC, Mast Y. Challenging old microbiological treasures for natural compound biosynthesis capacity. Front Bioeng Biotechnol. 2024;12:1255151. doi:10.3389/fbioe.2024.1255151. ⇓

Castro-Falcón G, Straetener J, Bornikoel J, Reimer D, Purdy TN, Berscheid A, Schempp FM, Liu DY, Linington RG, Brötz-Oesterhelt H, Hughes CC. Antibacterial Marinopyrroles and Pseudilins act as protonophores. ACS Chem Biol. (2024). doi: 10.1021/acschembio.3c00773.⇓

Torres Salazar BO, Dema T, Schilling NA, Janek D, Bornikoel J, Berscheid A, Elsherbini AMA, Krauss S, Jaag SJ, Lämmerhofer M, Li M, Alqahtani N, Horsburgh MJ, Weber T, Beltrán-Beleña JM, Brötz-Oesterhelt H, Grond S, Krismer B, Peschel A. Commensal production of a broad-spectrum and short-lived antimicrobial peptide polyene eliminates nasal Staphylococcus aureus. Nat Microbiol. (2024) 9(1):200-213. doi: 10.1038/s41564-023-01544-2. ⇓

Bianchi M, Winterhalter M, Harbig TA, Hörömpöli D, Ghai I, Nieselt K, Brötz-Oesterhelt H, Mayer Ch, Borisova-Mayer M. Fosfomycin uptake in Escherichia coli is mediated by the outer-membrane porins OmpF, OmpC, and LamB. ACS Infect. Dis. (2023) doi: 10.1021/acsinfecdis.3c00367. ⇓

Hansen MH, Adamek M, Iftime D, Petras D, Schuseil F, Grond S, Stegmann E, Cryle MJ, Ziemert N. Resurrecting ancestral antibiotics: unveiling the origins of modern lipid II targeting glycopeptides. (2023) Nat Commun. 14:7842. doi: 10.1038/s41467-023-43451-4. ⇓

Goldfinger V, Spohn M, Rodler JP, Sigle M, Kulik A, Cryle MJ, Rapp J, Link H, Wohlleben W, Stegmann E (2023) Metabolic engineering of the shikimate pathway in Amycolatopsis strains for optimized glycopeptide antibiotic production. Metab Eng. 78:84-92. doi: 10.1016/j.ymben.2023.05.005. ⇓

Stincone P, Shah AKP, Schmid R, Graves L, Lambidis SP, Torres R, Xia SN, Minda V, Aron A, Wang M, Hughes CC, Petras D. Evaluation of data dependent MS/MS acquisition parameters for non-targeted metabolomics and molecular networking of environmental samples: Focus on the Q Exactive platform. Anal Chem. 2023;95:12673-12682. doi:10.1021/acs.analchem.3c01202. ⇓

Illigmann A, Vielberg MT, Lakemeyer M, Wolf F, Dema T, Stange P, Kuttenlochner W, Liebhart E, Kulik A, Staudt N, Malik I, Grond S, Sieber SA, Kaysser L, Groll M, Brötz-Oesterhelt H. Structure of Staphylococcus aureus ClpP Bound to the Covalent Active Site Inhibitor Cystargolide A. Angew Chem Int Ed Engl. e202314028 (2023). ⇓

Brötz-Oesterhelt H, Hughes C, Sass P, Stegmann E, Ziemert N. Aktuelle Methoden in der antibakteriellen Naturstoffforschung. Biospektrum 29, 599–601 (2023). ⇓

Peslalz P, Grieshober M, Kraus F, Bleisch A, Izzo F, Lichtenstein D, Hammer H, Vorbach A, Momoi K, Zanger UM, Brötz-Oesterhelt H, Braeuning A, Plietker B, Stenger S. Unnatural Endotype B PPAPs as Novel Compounds with Activity against Mycobacterium tuberculosis. J Med Chem. doi: 10.1021/acs.jmedchem.3c01172. (2023). ⇓

Ropponen HK, Diamanti E, Johannsen S, Illarionov B, Hamid R, Jaki M, Sass P, Fischer M, Haupenthal J, Hirsch AKH. Exploring the Translational Gap of a Novel Class of Escherichia coli IspE Inhibitors. ChemMedChem. e202300346. doi: 10.1002/cmdc.202300346. (2023). ⇓

Ho YTC, Schittenhelm RB, Iftime D, Stegmann E, Tailhades J, Cryle MJ (2023) Exploring the flexibility of the glycopeptide antibiotic crosslinking cascade for extended peptide backbones. Chembiochem. 24(6):e202200686. doi: 10.1002/cbic.202200686. ⇓

Hansen MH, Stegmann E, Cryle MJ (2022) Beyond vancomycin: recent advances in the modification, reengineering, production and discovery of improved glycopeptide antibiotics to tackle multidrug-resistant bacteria. Curr Opin Biotechnol. 77:102767. doi: 10.1016/j.copbio.2022.102767. ⇓

Saad H, Majer T, Bhattarai K, Lampe S, Nguyen DT, Kramer M, Straetener J, Brötz-Oesterhelt H, Mitchell DA, Gross H. Bioinformatics-guided discovery of biaryl-linked lasso peptides. Chem Sci. 14(45):13176-13183 (2023). ⇓

Wilkinson IVL, Castro-Falcón G, Roda-Serrat MC, Purdy TN, Straetener J, Brauny MM, Maier L, Brötz-Oesterhelt H, Christensen LP, Sieber SA, Hughes CC. The Cyanobacterial "Nutraceutical" Phycocyanobilin Inhibits Cysteine Protease Legumain. Chembiochem. 24(5):e202200455 (2023). ⇓

Walther R, Westermann LM, Carmali S, Jackson SE, Brötz-Oesterhelt H, Spring DR. Identification of macrocyclic peptides which activate bacterial cylindrical proteases. RSC Med Chem. 14:1186-1191. doi: 10.1039/d3md00136a. (2023). ⇓ 

Pan S, Jensen AA, Wood NA, Henrichfreise B, Brötz-Oesterhelt H, Fisher DJ, Sass P, Ouellette SP. Molecular characterization of the ClpC AAA+ ATPase in the biology of Chlamydia trachomatis. mBio e0007523 (2023). ⇓

Gratani FL, Englert T, Nashier P, Sass P, Mann P, Czech L, Neumann N, Doello S, Blobelt R, Alberti S, Forchhammer K, Bange G, Höfer K, Macek B. E. coli toxin YjjJ (HipH) is a Ser/Thr protein kinase that impacts cell division, carbon metabolism and ribosome assembly. mSystems e0104322 (2023). ⇓

Moschny J, Daletos G, Proksch P, Hughes CC. Structure elucidation of antibiotics. Antibiotics: Methods in Molecular Biology. Vol 2601. (Sass P, ed.). Humana Press Inc.; 2023. doi:10.1002/0471739499.oth1. ⇓

Matos de Opitz CL, Sass P. Microscopy-based multiwell assay to characterize disturbed bacterial morphogenesis upon antibiotic action. In Peter Sass (ed.), Antibiotics: Methods and Protocols, Methods in Molecular Biology 2nd edition, vol. 2601. Springer, New York. (2023). ⇓

Brajtenbach D, Puls JS, Matos de Opitz CL, Sass P, Kubitscheck U, Grein F. Quantitative analysis of microscopy data to evaluate bacterial responses to antibiotic treatment. In Peter Sass (ed.), Antibiotics: Methods and Protocols, Methods in Molecular Biology 2nd edition, vol. 2601. Springer, New York. (2023). ⇓

Sass P. Antibiotics - Precious goods in changing times (update 2023). In Peter Sass (ed.), Antibiotics: Methods and Protocols, Methods in Molecular Biology 2nd edition, vol. 2601. Springer, New York. In press (2023). ⇓

2020-2022

Bär, D.; B. Konetschny, A. Kulik, H. Xu, D. Paccagnella, P. Beller, N. Ziemert, J. S. Dickschat, B. Gust. Origin of the 3-methylglutaryl moiety in caprazamycin biosynthesis. Microb Cell Fact. 2022 Nov 5. 21:232. doi: 10.1186/s12934-022-01955-6.

Reinhardt L, Thomy D, Lakemeyer M, Westermann LM, Ortega J, Sieber SA, Sass P, Brötz-Oesterhelt H. Antibiotic Acyldepsipeptides Stimulate the Streptomyces Clp-ATPase/ClpP Complex for Accelerated Proteolysis. mBio. 2022 Oct 26. doi: 10.1128/mbio.01413-22.

Zhang L, Esquembre LA, Xia SN, Oesterhelt F, Hughes CC, Brötz-Oesterhelt H, Teufel R. Antibacterial Synnepyrroles from Human-Associated Nocardiopsis sp. Show Protonophore Activity and Disrupt the Bacterial Cytoplasmic Membrane. ACS Chem Biol. 2022 Sep 30. doi: 10.1021/acschembio.2c00460.

Dietrich A, Steffens U, Gajdiss M, Boschert AL, Dröge JK, Szekat C, Sass P, Malik IT, Bornikoel J, Reinke L, Maček B, Franz-Wachtel M, Nieselt K, Harbig T, Scherlach K, Brötz-Oesterhelt H, Hertweck C, Sahl HG, Bierbaum G. Cervimycin-Resistant Staphylococcus aureus Strains Display Vancomycin-Intermediate Resistant Phenotypes. Microbiol Spectr. 2022 Sep 29:e0256722. doi: 10.1128/spectrum.02567-22.

Xu H, Wochele A, Luo M, Schnakenburg G, Sun Y, Brötz-Oesterhelt H, Dickschat JS. Synthesis of tryptophan-dehydrobutyrine diketopiperazine and biological activity of hangtaimycin and its co-metabolites. Beilstein J Org Chem. 2022 Sep 7;18:1159-1165. doi: 10.3762/bjoc.18.120.

Majer T, Bhattarai K, Straetener J, Pohlmann J, Cahill P, Zimmermann MO, Hübner MP, Kaiser M, Svenson J, Schindler M, Brötz-Oesterhelt H, Boeckler FM, Gross H. Discovery of Ircinianin Lactones B and C-Two New Cyclic Sesterterpenes from the Marine Sponge Ircinia wistarii. Mar Drugs. 2022 Aug 19;20(8):532. doi: 10.3390/md20080532.

Reher R, Aron AT, Fajtová P, Stincone P, Wagner B, Pérez-Lorente AI, Liu C, Shalom IYB, Bittremieux W, Wang M, Jeong K, Matos-Hernandez ML, Alexander KL, Caro-Diaz EJ, Naman CB, Scanlan JHW, Hochban PMM, Diederich WE, Molina-Santiago C, Romero D, Selim KA, Sass P, Brötz-Oesterhelt H, Hughes CC, Dorrestein PC, O'Donoghue AJ, Gerwick WH, Petras D. Native metabolomics identifies the rivulariapeptolide family of protease inhibitors. Nat Commun. 2022 Aug 8;13(1):4619. doi: 10.1038/s41467-022-32016-6.

Handel F, Kulik A, Wex KW, Berscheid A, Saur JS, Winkler A, Wibberg D, Kalinowski J, Brötz-Oesterhelt H, Mast Y. Ψ-Footprinting approach for the identification of protein synthesis inhibitor producers. NAR Genom Bioinform. 2022 Jul 15;4(3):lqac055. doi: 10.1093/nargab/lqac055.

Kim L, Lee BG, Kim M, Kim MK, Kwon DH, Kim H, Brötz-Oesterhelt H, Roh SH, Song HK. Structural insights into ClpP protease side exit pore-opening by a pH drop coupled with substrate hydrolysis. EMBO J. 2022 May 20:e109755. doi: 10.15252/embj.2021109755.

Mendes SS, Marques J, Mesterházy E, Straetener J, Arts M, Pissarro T, Reginold J, Berscheid A, Bornikoel J, Kluj RM, Mayer C, Oesterhelt F, Friães S, Royo B, Schneider T, Brötz-Oesterhelt H, Romão CC, Saraiva LM. Synergetic Antimicrobial Activity and mechanism of Clotrimazole-Linked CO-Releasing Molecules. ACS Bio & Med Chem. 2022 April 8 doi: 10.1021/acsbiomedchemau.2c00007.

Yin Z, Liebhart E, Stegmann E, Brötz-Oesterhelt H, Dickschat J. An Isotopic Probe to Follow the Stereochemical Course of Dehydratase Reactions in Polyketide and Fatty Acid Biosynthesis. Organic Chemistry Frontiers. 2022 April 7. doi: 10.1039/D2QO00272H.

Gottstein J, Zaschke-Kriesche J, Unsleber S, Voitsekhovskaia I, Kulik A, Behrmann LV, Overbeck N, Stühler K, Stegmann E, Smits SHJ. New insights into the resistance mechanism for the BceAB-type transporter SaNsrFP. Sci Rep. 2022 Mar 10;12(1):4232. doi: 10.1038/s41598-022-08095-2.

Krysenko S, Okoniewski N, Nentwich M, Matthews A, Bäuerle M, Zinser A, Busche T, Kulik A, Gursch S, Kemeny A, Bera A, Wohlleben W. A Second Gamma-Glutamylpolyamine Synthetase, GlnA2, Is Involved in Polyamine Catabolism in Streptomyces coelicolor. Int J Mol Sci. 2022 Mar 29;23(7):3752. doi: 10.3390/ijms23073752.

Aryal N, Chen J, Bhattarai K, Hennrich O, Handayani I, Kramer M, Straetener J, Wommer T, Berscheid A, Peter S, Reiling N, Brötz-Oesterhelt H, Geibel C, Lämmerhofer M, Mast Y, Gross H. High Plasticity of the Amicetin Biosynthetic Pathway in Streptomyces sp. SHP 22-7 Led to the Discovery of Streptcytosine P and Cytosaminomycins F and G and Facilitated the Production of 12F-Plicacetin. J Nat Prod. 2022 Mar 9. doi: 10.1021/acs.jnatprod.1c01051.

Malik IT, Hegemann JD, Brötz-Oesterhelt H. Generation of Lasso Peptide-Based ClpP Binders. Int. J. Mol. Sci.. 2022; 23(1):465. doi: 10.3390/ijms23010465.

Förster C, Handrick V, Ding Y, Nakamura Y, Paetz C, Schneider B, Castro-Falcón G, Hughes CC, Luck K, Poosapati S, Kunert G, Huffaker A, Gershenzon J, Schmelz EA, Köllner TG. Biosynthesis and antifungal activity of fungus-induced O-methylated flavonoids in maize. Plant Physiol. 2021 Oct 27:kiab496. doi: 10.1093/plphys/kiab496.

Beck C, Gren T, Ortiz-López FJ, Jørgensen TS, Carretero-Molina D, Martín Serrano J, Tormo JR, Oves-Costales D, Kontou EE, Mohite OS, Mingyar E, Stegmann E, Genilloud O, Weber T. Activation and Identification of a Griseusin Cluster in Streptomyces sp. CA-256286 by Employing Transcriptional Regulators and Multi-Omics Methods. Molecules. 2021 Oct 30;26(21):6580. doi: 10.3390/molecules26216580.

Tuttle RN, Rouse GW, Castro-Falcón G, Hughes CC, Jensen PR. Specialized Metabolite Mediated Predation Defense in the Marine Actinobacterium Salinispora. Appl Environ Microbiol. 2021 Oct 20:AEM0117621. doi: 10.1128/AEM.01176-21.

Hughes CC. Chemical labeling strategies for small molecule natural product detection and isolation. Nat Prod Rep. 2021 Sep 23;38(9):1684-1705. doi: 10.1039/d0np00034e.

Illigmann A, Thoma Y, Pan S, Reinhardt L, Brötz-Oesterhelt H. Contribution of the Clp Protease to Bacterial Survival and Mitochondrial Homoeostasis. Microb Physiol 2021. doi: 10.1159/000517718.

Miethke M, Pieroni M, Weber T, Brönstrup M, Hammann P, Halby L, Arimondo P B, Glaser P, Aigle B, Bode H B, Moreira R, Li Y, Luzhetskyy A, Medema M H, Pernodet J-L, Stadler M, Tormo J R, Genilloud O, Truman A W, Weissman K J, Takano E, Sabatini S, Stegmann E, Brötz-Oesterhelt H, Wohlleben W, Seemann M, Empting M, Hirsch A K H, Loretz B, Lehr C-M, Titz A, Herrmann J, Jaeger T, Alt S, Hesterkamp T, Winterhalter M, Schiefer A, Pfarr K, Hoerauf A, Graz H, Graz M, Lindvall M, Ramurthy S, Karlén A, van Dongen M, Petkovic H, Keller A, Peyrane F, Donadio S, Fraisse L, Piddock L J V, Gilbert I H, Moser H E, Müller R. Towards the sustainable discovery and development of new antibiotics. Nature Reviews Chemistry 2021. doi:10.1038/s41570-021-00313-1.

Engelbrecht A, Wolf F, Esch A, Kulik A, Kozhushkov SI, de Meijere A, Hughes CC, Kaysser L. Discovery of a Cryptic Nitro Intermediate in the Biosynthesis of the 3-(trans-2'-Aminocyclopropyl)alanine Moiety of Belactosin A. Org Lett. 2022 Jan 21;24(2):736-740. doi: 10.1021/acs.orglett.1c04205.

Mingyar E, Mühling L, Kulik A, Winkler A, Wibberg D, Kalinowski J, Blin K, Weber T, Wohlleben W, Stegmann E. A Regulator Based "Semi-Targeted" Approach to Activate Silent Biosynthetic Gene Clusters. Int J Mol Sci. 2021 Jul 15;22(14):7567. doi: 10.3390/ijms22147567.

Heilbronner S, Krismer B, Brötz-Oesterhelt H, Peschel A.. The microbiome-shaping roles of bacteriocins. Nat Rev Microbiol. 2021 June 1. doi: 10.1038/s41579-021-00569-w.

Botas A, Eitel M, Schwarz PN, Buchmann A, Costales P, Núñez LE, Cortés J, Morís F, Krawiec M, Wolański M, Gust B, Rodriguez M, Fischer WN, Jandeleit B, Zakrzewska-Czerwińska J, Wohlleben W, Stegmann E, Koch P, Méndez C, Gross H. Genetic Engineering in Combination with Semi-Synthesis Leads to a New Route for Gram-Scale Production of the Immunosuppressive Natural Product Brasilicardin A. Angew Chem Int Ed Engl. 2021 Jun 7;60(24):13536-13541. doi: 10.1002/anie.202015852.

Saad H, Aziz S, Gehringer M, Kramer M, Straetener J, Berscheid A, Brötz-Oesterhelt H, Gross H. Nocathioamides, uncovered by a Tunable Metabologenomic Approach, define a Novel Class of Chimeric Lanthipeptides. Angew Chem Int Ed Engl. 2021 May 14. doi: 10.1002/anie.202102571.

Brötz-Oesterhelt H, Vorbach A. Reprogramming of the Caseinolytic Protease by ADEP Antibiotics: Molecular Mechanism, Cellular Consequences, Therapeutic Potential. Frontiers in Molecular Biosciences. 2021 May 13. doi: 10.3389/fmolb.2021.690902.

Koeninger L, Osbelt L, Berscheid A, Wendler J, Berger J, Hipp K, Lesker TR, Pils MC, Malek NP, Jensen BAH, Brötz-Oesterhelt H, Strowig T, Wehkamp J. Curbing gastrointestinal infections by defensin fragment modifications without harming commensal microbiota. Commun Biol. 2021 Jan 8;4(1):47. doi: 10.1038/s42003-020-01582-0.

Frediansyah A, Straetener J, Brötz-Oesterhelt H, Gross H. Massiliamide, a cyclic tetrapeptide with potent tyrosinase inhibitory properties from the Gram-negative bacterium Massilia albidiflava DSM 17472^T. J Antibiot (Tokyo). 2021 74(4):269-272. doi: 10.1038/s41429-020-00394-y.

Ortlieb N, Klenk E, Kulik A, Niedermeyer THJ. Development of an agar-plug cultivation system for bioactivity assays of actinomycete strain collections. PLoS One. 2021 Nov 5;16(11):e0258934. doi: 10.1371/journal.pone.0258934.

Shimo S, Ushimaru R, Engelbrecht A, Harada M, Miyamoto K, Kulik A, Uchiyama M, Kaysser L, Abe I. Stereodivergent Nitrocyclopropane Formation during Biosynthesis of Belactosins and Hormaomycins. J Am Chem Soc. 2021 Nov 10;143(44):18413-18418. doi: 10.1021/jacs.1c10201.

Wex KW, Saur JS, Handel F, Ortlieb N, Mokeev V, Kulik A, Niedermeyer THJ, Mast Y, Grond S, Berscheid A, Brötz-Oesterhelt H. Bioreporters for direct mode of action-informed screening of antibiotic producer strains. Cell Chem Biol. 2021 doi: 10.1016/j.chembiol.2021.02.022.

Mitousis L, Maier H, Martinovic L, Kulik A, Stockert S, Wohlleben W, Stiefel A, Musiol-Kroll EM. Engineering of Streptoalloteichus tenebrarius 2444 for Sustainable Production of Tobramycin. Molecules. 2021 Jul 18;26(14):4343. doi: 10.3390/molecules26144343.

Silber N, Mayer C, Matos de Opitz CL, Sass P. Progression of the late-stage divisome is unaffected by the depletion of the cytoplasmic FtsZ pool. Commun Biol 4, 270 (2021).⇓

Dietrich A, Steffens U, Sass P, Bierbaum G. The hypersusceptible antibiotic screening strain Staphylococcus aureus SG511-Berlin harbors multiple mutations in regulatory genes. Int J Med Microbiol. 311(8):151545 (2021). ⇓

Hörömpöli D, Ciglia C, Glüsenkamp KH, Haustedt LO, Falkenstein-Paul H, Bendas G, Berscheid A, Brötz-Oesterhelt H. The antibiotic negamycin crosses the bacterial cytoplasmic membrane by multiple routes. Antimicrob Agents Chemother. 2021;AAC.00986-20. doi:10.1128/AAC.00986-20.

Handayani I, Saad H, Ratnakomala S, Lisdiyanti P, Kusharyoto W, Krause J, Kulik A, Wohlleben W, Aziz S, Gross H, Gavriilidou A, Ziemert N, Mast Y. Mining Indonesian Microbial Biodiversity for Novel Natural Compounds by a Combined Genome Mining and Molecular Networking Approach. Mar Drugs. 2021 May 28;19(6):316. doi: 10.3390/md19060316.

Wolański M, Krawiec M, Schwarz PN, Stegmann E, Wohlleben W, Buchmann A, Gross H, Eitel M, Koch P, Botas A, Méndez C, Núñez LE, Morís F, Cortés J, Zakrzewska-Czerwińska J. A novel LysR-type regulator negatively affects biosynthesis of the immunosuppressant brasilicardin. Eng Life Sci. 2020 Nov 4;21(1-2):4-18. doi: 10.1002/elsc.202000038.

Chen J, Frediansyah A, Männle D, Straetener J, Brötz-Oesterhelt H, Ziemert N, Kaysser L, Gross H. New Nocobactin Derivatives with Antimuscarinic Activity, Terpenibactins A-C, Revealed by Genome Mining of Nocardia terpenica IFM 0406. Chembiochem. 2020;21(15):2205-2213. doi:10.1002/cbic.202000062.

Silber N, Matos de Opitz CL, Mayer C, Sass P. Cell division protein FtsZ: from structure and mechanism to antibiotic target. Fut Microbiol. doi: 10.2217/fmb-2019-0348 (2020).

Matos de Opitz CL, Sass P. Tackling antimicrobial resistance by exploring new mechanisms of antibiotic action. Fut Microbiol. doi: 10.2217/fmb-2020-0048 (2020).

Senges CHR, Stepanek JJ, Wenzel M, Raatschen N, Ay Ü, Märtens Y, Prochnow P, Vázquez Hernández M, Yayci A, Schubert B, Janzing NBM, Warmuth HL, Kozik M, Bongard J, Alumasa JN, Albada B, Penkova M, Lukežič T, Sorto NA, Lorenz N, Miller R, Zhu B, Benda M, Stülke J, Schäkermann S, Leichert LI, Scheinpflug K, Brötz-Oesterhelt H, Hertweck C, Shaw JT, Petković H, Brunel JM, Keiler KC, Metzler-Nolte N, Bandow JE. Comparison of proteomic responses as global approach to antibiotic mechanism of action elucidation. Antimicrob Agents Chemother. 2020 Oct 12:AAC.01373-20. PMID: 33046497. doi: 10.1128/AAC.01373-20.

Silber N, Pan S, Schäkermann S, Mayer C, Brötz-Oesterhelt H, Sass P. Cell division protein FtsZ is unfolded for N-terminal degradation by antibiotic-activated ClpP. mBio 11(3):e01006-20. https://doi.org/10.1128/mBio.01006-20.(2020). ⇓

Bafna JA, Sans-Serramitjana E, Acosta-Gutiérrez S, Bodrenko IV, Hörömpöli D, Berscheid A, Brötz-Oesterhelt H, Winterhalter M, Ceccarelli M. Kanamycin Uptake into Escherichia coli Is Facilitated by OmpF and OmpC Porin Channels Located in the Outer Membrane. ACS Infect. Dis. 2020. doi: 10.1021/acsinfecdis.0c00102. 

Handel F, Kulik A, Mast Y. Investigation of the Autoregulator-Receptor System in the Pristinamycin Producer Streptomyces pristinaespiralis. Front Microbiol. 2020 Sep 30;11:580990. doi: 10.3389/fmicb.2020.580990.

Malik IT, Pereira R, Vielberg MT, Mayer C, Straetener J, Thomy D, Famulla K, Castro H, Sass P, Groll M, Brötz-Oesterhelt H. Functional characterisation of ClpP mutations conferring resistance to acyldepsipeptide antibiotics in Firmicutes. Chembiochem. 2020. doi: 10.1002/cbic.201900787. ⇓

Edenhart S, Denneler M, Spohn M, Doskocil E, Kavšček M, Amon T, Kosec G, Smole J, Bardl B, Biermann M, Roth M, Wohlleben W, Stegmann E. Metabolic engineering of Amycolatopsis japonicum for optimized production of [S,S]-EDDS, a biodegradable chelator. Metab Eng. 2020 Jul;60:148-156. doi: 10.1016/j.ymben.2020.04.003.

Mast Y, Stegmann E, Lu Y. Editorial: Regulation of Antibiotic Production in Actinomycetes. Front Microbiol. 2020 Jul 22;11:1566. doi: 10.3389/fmicb.2020.01566.

Krause J, Handayani I, Blin K, Kulik A, Mast Y. Disclosing the Potential of the SARP-Type Regulator PapR2 for the Activation of Antibiotic Gene Clusters in Streptomycetes. Front Microbiol. 2020 Feb 18;11:225. doi: 10.3389/fmicb.2020.00225.

Moosmann D, Mokeev V, Kulik A, Osipenkov N, Kocadinc S, Ort-Winklbauer R, Handel F, Hennrich O, Youn JW, Sprenger GA, Mast Y. Genetic engineering approaches for the fermentative production of phenylglycines. Appl Microbiol Biotechnol. 2020 Apr;104(8):3433-3444. doi: 10.1007/s00253-020-10447-9.

Chen J, Frediansyah A, Männle D, Straetener J, Brötz-Oesterhelt H, Ziemert N, Kaysser L, Gross H. Terpenibactins A-C, new nocobactin derivatives with antimuscarinic activity, revealed by genome mining of Nocardia terpenica IFM 0406. Chembiochem. 2020. doi: 10.1002/cbic.202000062. ⇓

Kossack R, Breinlinger S, Nguyen T, Moschny J, Straetener J, Berscheid A, Brötz-Oesterhelt H, Enke H, Schirmeister T, Niedermeyer THJ. Nostotrebin 6 related cyclopentenediones and δ-lactones with broad activity spectrum isolated from the cultivation medium of the cyanobacterium Nostoc sp. CBT1153 J. Nat. Prod. doi: 10.1021/acs.jnatprod.9b00885. ⇓

Tribelli PM, Luqman A, Nguyen MT, Madlung J, Fan SH, Macek B, Sass P, Bitschar K, Schittek B, Kretschmer D, Götz F. Staphylococcus aureus Lpl protein triggers human host cell invasion via activation of Hsp90 receptor. Cell Microbiol. 22(1):e13111 (2020).

2015-2019

Ding Y, Murphy KM, Poretsky E, Mafu S, Yang B, Char SN, Christensen SA, Saldivar E, Wu M, Wang Q, Ji L, Schmitz RJ, Kremling KA, Buckler ES, Shen Z, Briggs SP, Bohlmann J, Sher A, Castro-Falcon G, Hughes CC, Huffaker A, Zerbe P, Schmelz EA. Multiple genes recruited from hormone pathways partition maize diterpenoid defences. Nat Plants. 2019 Oct;5(10):1043-1056. doi: 10.1038/s41477-019-0509-6.

Vollmer B, Steblau N, Ladwig N, Mayer C, Macek B, Mitousis L, Sigle S, Walter A, Wohlleben W, Muth G. Role of the Streptomyces spore wall synthesizing complex SSSC in differentiation of Streptomyces coelicolor A3(2). Int J Med Microbiol. 2019 Sep;309(6):151327. doi: 10.1016/j.ijmm.2019.07.001.

Seiler GS, Hughes CC. Progress toward the Total Synthesis of Lymphostins: Preparation of a Functionalized Tetrahydropyrrolo[4,3,2-de]quinoline and Unusual Oxidative Dimerization. J Org Chem. 2019 Jul 19;84(14):9339-9343. doi: 10.1021/acs.joc.9b01041.

Schafhauser T, Jahn L, Kirchner N, Kulik A, Flor L, Lang A, Caradec T, Fewer DP, Sivonen K, van Berkel WJH, Jacques P, Weber T, Gross H, van Pée KH, Wohlleben W, Ludwig-Müller J. Antitumor astins originate from the fungal endophyte Cyanodermella asteris living within the medicinal plant Aster tataricus. Proc Natl Acad Sci U S A. 2019 Dec 6;116(52):26909–17. doi: 10.1073/pnas.1910527116.

Thoma L, Vollmer B, Oesterhelt F, Muth G. Live-cell imaging of Streptomyces conjugation. Int J Med Microbiol. 2019 Jul;309(5):338-343. doi: 10.1016/j.ijmm.2019.05.006.

Eyermann B, Meixner M, Brötz-Oesterhelt H, Antes I, Sieber SA. Acyldepsipeptide probes facilitate specific detection of caseinolytic protease P independent of its oligomeric and activity state. Chembiochem. doi: 10.1002/cbic.201900477. ⇓

Lennings J, Makhlouf M, Olejnik P, Mayer C, Brötz-Oesterhelt H, Schwarz S. Environmental and cellular factors affecting the localization of T6SS proteins in Burkholderia thailandensis. Int J Med Microbiol. 2019 Sep;309(6):151335. doi: 10.1016/j.ijmm.2019.151335. (2019). ⇓

Moss NA, Seiler G, Leão TF, Castro-Falcón G, Gerwick L, Hughes CC, Gerwick WH. Nature's Combinatorial Biosynthesis Produces Vatiamides A-F. Angew Chem Int Ed Engl. 2019 Jul 1;58(27):9027-9031. doi: 10.1002/anie.201902571.

Pan S, Malik IT, Thomy D, Henrichfreise B, Sass P. The functional ClpXP protease of Chlamydia trachomatis requires distinct clpP genes from separate genetic loci. Sci Rep. 9(1):14129. doi: 10.1038/s41598-019-50505-5 (2019).

Thomy D, Culp E, Adamek M, Cheng EY, Ziemert N, Wright GD, Sass P, Brötz-Oesterhelt H. The ADEP biosynthetic gene cluster in Streptomyces hawaiiensis NRRL 15010 reveals an accessory clpP gene as a novel antibiotic resistance factor. Appl. Environ. Microbiol. doi:10.1128/AEM.01292-19 (2019). ⇓ [For a free e-print, please contact us]

Mayer C, Sass P, Brötz-Oesterhelt H. Consequences of dosing and timing on the antibacterial effects of ADEP antibiotics. Int J Med Microbiol. 151329. doi: 10.1016/j.ijmm.2019.151329 (2019). ⇓

Luqman A, Ebner P, Reichert S, Sass P, Kabagema-Bilan C, Heilmann C, Ruth P, Götz F. A new host cell internalisation pathway for SadA-expressing staphylococci triggered by excreted neurochemicals. Cell Microbiol. e13044. doi: 10.1111/cmi.13044 (2019).

Schilling NA, Berscheid A, Schumacher J, Saur JS, Konnerth MC, Wirtz SN, Beltrán Beleña JM, Zipperer A, Krismer B, Peschel A, Kalbacher H, Brötz-Oesterhelt H, Steinem C, Grond SC. 2019. Synthetic lugdunin analogues reveal essential structural motifs for antimicrobial action and proton translocation capability. Angew Chem Int Ed Engl. 2019 May 6. doi: 10.1002/anie.201901589. [Epub ahead of print]. ⇓

Sass P. Molekulare Antibiotikaforschung: neue Wirkprinzipien gegen multiresistente Keime. Biospektrum 25: 463. doi: 10.1007/s12268-019-1076-0 (2019).

Wang H, Kraus F, Popella P, Baykal A, Guttroff C, François P, Sass P, Plietker B, Götz F. The polycyclic polyprenylated acylphloroglucinol antibiotic PPAP 23 targets the membrane and iron metabolism in Staphylococcus aureus. Front Microbiol doi: 10.3389/fmicb.2019.00014 (2019).

Lennings J, Mayer C, Makhlouf M, Brötz-Oesterhelt H, Schwarz S. 2019. Polar localization of the ATPase ClpV-5 occurs independent of type VI secretion system apparatus proteins in Burkholderia thailandensis. BMC Res Notes. 2019 Feb 28;12(1):109. doi: 10.1186/s13104-019-4141-3. ⇓

Moreno-Cinos C, Sassetti E, Salado IG, Witt G, Benramdane S, Reinhardt L, Durante Cruz C, Joossens J, Van der Veken P, Brotz-Oesterhelt H, Tammela P, Winterhalter M, Gribbon P, Windshügel B, Augustyns K. 2018. α-Amino diphenyl phosphonates as novel inhibitors of Escherichia coli ClpP protease. J Med Chem. 2018 Dec 20. doi: 10.1021/acs.jmedchem.8b01466. ⇓

Miess H, Jahanshah G, Brötz-Oesterhelt H, Willmann M, Peter S, Gross H. 2018. Draft Genome Sequence of the Extensively Drug-Resistant Pseudomonas aeruginosa Clinical Isolate TUEPA7472. Microbiol Resour Announc 7:e01055-18. ⇓

Saising J, Nguyen MT, Härtner T, Ebner P, Al Mamun Bhuyan A, Berscheid A, Muehlenkamp M, Schäkermann S, Kumari N, Maier ME, Voravuthikunchai SP, Bandow J, Lang F, Brötz-Oesterhelt H, Götz F. 2018. Rhodomyrtone (Rom) is a membrane-active compound. Biochim Biophys Acta., 1860(5):1114-1124. ⇓

Reichert S, Ebner P, Bonetti EJ, Luqman A, Nega M, Schrenzel J, Spröer C, Bunk B, Overmann J, Sass P, François P, Götz F. Genetic adaptation of a mevalonate pathway deficient mutant in Staphylococcus aureus. Front Microbiol doi: 10.3389/fmicb.2018.01539 (2018).

Perez R, Wörmer LP, Sass P, Maldener, I. 2017. A highly asynchronous developmental program triggered during germination of dormant akinetes of the filamentous diazotrophic cyanobacteria. FEMS Microbiol Ecol. 94(1). doi: 10.1093/femsec/fix131 (2018).

Mokhlesi A, Stuhldreier F, Wex KW, Berscheid A, Hartmann R, Rehberg N, Sureechatchaiyan P, Chaidir C, Kassack M, Kalscheuer R, Brötz-Oesterhelt H, Wesselborg S, Stork B, Daletos G, Proksch P. 2017. Cyclic cystine-bridged peptides from the marine sponge Clathria basilana induce apoptosis in tumor cells and depolarize the bacterial cytoplasmic membrane. J Nat Prod., 80(11):2941-2952. 80(11):2941-2952. ⇓

Malik I, Brötz-Oesterhelt H. 2017. Conformational control of the bacterial Clp protease by natural product antibiotics. Nat. Prod. Rep., 34(7):815-831. ⇓

Raschig J, Mailänder-Sanchez D, Berscheid A, Berger J, Strömstedt AA, Courth L, Malek N, Brötz-Oesterhelt H, Wehkamp J. 2017. Redox dependent trapping and killing represent diverse antibacterial strategies of ubiquitously expressed human beta defensin 1 (hBD1). Plos Pathogens, 13(3):e1006261. ⇓

Schüller A, Matzner D, Lünse C, Wittmann V, Schumacher C, Unsleber S, Brötz-Oesterhelt H, Mayer C, Bierbaum G, Mayer G. 2017. "Activation of the glmS ribozyme confers bacterial growth inhibition." Chembiochem, 18(5):435-440. ⇓

Sass P. 2017. Antibiotics: Precious Goods in Changing Times. Methods Mol Biol. 2017;1520:3-22.

Sass P. 2017. Antibiotics: Methods and Protocols, Methods in Molecular Biology, vol. 1520, Peter Sass (ed.), DOI 10.1007/978-1-4939-6634-9_1, © Springer Science+Business Media New York 2017

Hardt P, Engels I, Rausch M, Gajdiss M, Ulm H, Sass P, Ohlsen K, Sahl HG, Bierbaum G, Schneider T, Grein F. 2017. The cell wall precursor lipid II acts as a molecular signal for the Ser/Thr kinase PknB of Staphylococcus aureus. Int. J. Med. Microbiol. 307(1):1-10. doi: 10.1016/j.ijmm.2016.12.001.

Zipperer, A. , Konnerth, M.C., Laux. C., Berscheid, A., Janek, D., Weidenmaier, C., Burian, M., Schilling, N.A., Slavetinsky, C., Marschal, M., Willmann, M., Kalbacher, H., Schittek, B., Brötz-Oesterhelt, H., Grond, S., Peschel, A., Krismer, B. 2016. „Human commensals producing a novel antibiotic impair pathogen colonization.“ Nature 535(7613):511-6. ⇓

Famulla, K., P. Sass, I. Malik, T. Akopian, O. Kandror, M. Alber, B. Hinzen, H. Ruebsamen-Schaeff, R. Kalscheuer, A.L. Goldberg, H. Brötz-Oesterhelt. 2016. „Acyldepsipeptide antibiotics kill mycobacteria by preventing the physiological functions of the ClpP1P2 protease.” Mol Microbiol. 101(2):194-209. ⇓

Gersch, M., Famulla, K., Dahmen, M., Göbl, C., Malik, I., Richter, K., Korotkoc, V.S., Sass, P., Rübsamen-Schaeff, H., Madl, T, Brötz-Oesterhelt, H., and Sieber, S. 2015. „AAA+ chaperones and acyldepsipeptides activate ClpP via conformational control.” Nat. Commun. 6:6320. ⇓

Chen, H., Daletos, G., Abdel-Aziz, M.S., Thomy, D., Dai, H., Brötz-Oesterhelt, H., Lin, W., Proksch, P. 2015. „Inducing secondary metabolite production by the soil-dwelling fungus Aspergillus terreus through bacterial co-culture” Phytochem. Letters 12:35-41.⇓
 

2010 - 2014

Hammerschmidt, L., Debbab, A., Ngocb, T.D., Wray, V., Perèz Hemphila, C., Lind, W., Brötz-Oesterhelt, H., Kassack, M., Proksch, P., Aly, A. H. 2014. „Polyketides from the mangrove-derived endophytic fungus Acremonium strictum.” Tetrahedron Lett. 55, 3463-3468. ⇓

Ola, A.R.B., Debbab, A., Kurtan, T., Brötz‐Oesterhelt, H., Aly, A.H., Proksch, P. 2014. “Dihydroanthracenone metabolites from the endophytic fungus Diaporthe melonis isolated from Annona squamosa.” Tetrahedron Lett. 55, 3147-3150. 3. ⇓

Ola, A.R.B., Debbab, A., Aly, A.H., Mandi, A., Zerfass, I., Hamacher, A., Kassack, M.U., Broetz-Oesterhelt, H., Kurtan, T., Proksch, P. 2014. “Absolute configuration and antibiotic activity of neosartorin from the endophytic fungus Aspergillus fumigatiaffinis.” Tetrahedron Letters (2014) 55: 1020-1023. ⇓

Wenzel, M., Chiriac, A. I., Otto, A., Zweytick, D., May, C., Schumacher, C., Gust, R., Bauke Albada, H., Penkova, M., Krämer, U., Erdmann, R., Metzler-Nolte, N., Straus, S., Bremer, E., Becher, D., Brötz-Oesterhelt, H., Sahl, H.-G. and Bandow, J. E. 2014. “Small cationic antimicrobial peptides delocalize peripheral membrane proteins.” Proc. Natl. Acad. Sci. (2014) doi:10.1073/pnas.1319900111. ⇓

Ola, A.R.B., Thomy, D., Lai, D., Brötz-Oesterhelt, H. and P. Proksch, H. “Inducing secondary metabolite production by the endophytic fungus Fusarium tricinctum through coculture with Bacillus subtilis.” J. Nat. Prod. 76, 2094-2099 (2013). ⇓

Sass, P., and H. Brötz-Oesterhelt, H. 2013. “Bacterial cell division as a target for new antibiotics.”Curr. Opin. Microbiol. 16, 522-530 (2013). ⇓

Brötz-Oesterhelt, H., and P. Sass. 2013. “Bacterial caseinolytic proteases as novel targets for antibacterial treatment.” Int J Med Microbiol. (2013), doi:pii: S1438-4221(13)00138-0. 10.1016/j.ijmm.2013.09.001. ⇓

Lai D., Brötz-Oesterhelt H., Müller W.E., Wray V., Proksch P. "Bioactive polyketides and alkaloids from Penicillium citrinum, a fungal endophyte isolated from Ocimum tenuiflorum." Fitoterapia. 91, 100-106 (2013). ⇓

Brötz-Oesterhelt, H. and P. Sass. Bacterial cell stress protein ClpP: A novel antibiotic target. In: Moonlighting cell stress proteins in microbial infections. B. Henderson (Ed.), Springer. “Moonlighting Cell Stress Proteins in Microbial Infections” in the series Heat Shock Proteins 7:375-385. Springer Netherlands, DOI 10.1007/978-94-007-6787-4_24, Print ISBN 978-94-007-6786-7, Online ISBN 978-94-007-6787-4 (2013).

Bara, R.; I. Zerfass, A.H. Aly, H. Goldbach-Gecke, V. Raghavan, P. Sass, A. Mándi, V. Wray, P.L. Polavarapu, A. Pretsch, W. Lin, T. Kurtán, A. Debbab, H. Brötz-Oesterhelt, and P. Proksch. 2013. “Atropisomeric dihydroanthracenones as inhibitors of multiresistant Staphylococcus aureus” J. Med. Chem., 56, 3257-3272 (2013). ⇓

Schiefer, A., J. Vollmer, C. Lämmer, S. Specht, C. Lentz, H. Ruebsamen-Schaeff, H. Brötz-Oesterhelt, A. Hoerauf und K. Pfarr. The peptidase ClpP of Wolbachia endobacteria is a novel target for drug development against filarial infections. J. Antimicrob. Chemother. 68, 1790-1800 (2013). ⇓

Lowth, B. R., Kirstein-Miles, J., Saiyet, T., Brötz-Oesterhelt, H., Morimoto, R. I., Truscott, K. N. and D. A. Dougan. Substrate recognition and processing by a Walker B mutant of the human mitochondrial AAA+ protein ClpX. J. Struct. Biol. 179, 193-201 (2012). ⇓

Müller, A., D. Münch, Y. Schmidt, K. Reder-Christ, G. Schiffer, G. Bendas, H. Gross, H.-G. Sahl, T. Schneider, H. Brötz-Oesterhelt. The lipodepsipeptide empedopeptin inhibits cell wall biosynthesis through Ca2+-dependent complex formation with peptidoglycan precursors. J Biol Chem. 287, 20270-20280 (2012). ⇓

Sass, P.*, A. Berscheid*, A. Jansen, M. Oedenkoven, C. Szekat, A. Strittmatter, G. Gottschalk, and G. Bierbaum. 2012. “Genome sequence of Staphylococcus aureus VC40, a vancomycin and daptomycin resistant strain, to study the genetics of development of resistance to currently applied last-resort antibiotics.” J. Bacteriol. (2012), 194(8):2107-08.

Berscheid, A.*, P. Sass*, K. Weber-Lasalle, A.L. Cheung, and G. Bierbaum. 2012. “Revisiting the genomes of the Staphylococcus aureus strains NCTC 8325 and RN4220.” Int J Med Microbiol. (2012), 302(2):84-87.

Sass, P. and H. Brötz-Oesterhelt. Bacterial stress responses to antimicrobial agents. In: Stress Responses in Foodborne Microorganisms, in the Series “Advances in Food Microbiology and Food Safety”. A. De Souza Sant’ana (Ed.). (2012) Nova Science Publishers Inc. ISBN: 978-1-61122-810-6.

Sass, P., M. Josten, K. Famulla, G. Schiffer, H.-G. Sahl, L. Hamoen and H. Brötz-Oesterhelt. Antibiotic acyldepsipeptides activate ClpP peptidase to degrade the cell division protein FtsZ. Proc. Natl. Acad. Sci. 108, 17474-17479 (2011). ⇓

Brötz-Oesterhelt, H., and P. Sass. 2011. Molekulare Antibiotikaforschung – Neue Leitstrukturen und Wirkmechanismen gegen multiresistente Bakterien. Annual reports of the Heinrich Heine University of Düsseldorf.

Brötz-Oesterhelt, H. and P. Sass. Post-genomic strategies in antibacterial drug discovery. Future Microbiology 5, 1553-1579 (2010). ⇓

Lee B.-G., E. Y. Park, K.-E. Lee, H. Jeon, K. H. Sung, H. Paulsen, H. Rübsamen-Schaeff, H. Brötz-Oesterhelt, and H. K. Song. Structures of ClpP in complex with acyldepsipeptide antibiotics reveal its activation mechanism. Nature Struct. Mol. Biol. 17, 471-478 (2010). ⇓

2005 - 2009

Kirstein, J., A. Hoffmann, H. Lilie, R. Schmidt, H. Rübsamen-Waigmann, H. Brötz-Oesterhelt, A. Mogk and K. Turgay. The antibiotic ADEP reprograms ClpP, switching it from a regulated to an uncontrolled protease. EMBO Molecular Medicine 1, 37-49 (2009). ⇓

Streker, K., T. Schäfer, C. Freiberg, H. Brötz-Oesterhelt, J. Hacker, H. Labischinski and K. Ohlsen. In vitro and in vivo validation of ligA and tarI as essential targets in Staphylococcus aureus. Antimicrob. Agents Chemother. 52, 4470-4474 (2008). ⇓

Brötz-Oesterhelt, H. and N. Brunner. How many modes of action should an antibiotic have? Curr. Opin. Pharmacol. 8:1-10 (2008). ⇓

Beyer, D., H-P. Kroll and H. Brötz-Oesterhelt. Methods to assay inhibitors of tRNA synthetase activity. In Methods in Molecular Medicine. Vol. 142, New antibiotic targets. S. Champney (Ed.), Humana Press, 53-61 (2008). ⇓

B. Hinzen, S. Raddatz, H. Paulsen, T. Lampe, A. Schumacher, D. Häbich, V. Hellwig, J. Benet-Buchholz, R. Endermann, H. Labischinski and H. Brötz-Oesterhelt. Medicinal chemistry optimization of acyldepsipeptides of the enopeptin class antibiotics. Chem. Med. Chem. 1, 689-693 (2006). ⇓

Labischinski H., C. Freiberg and H. Brötz-Oesterhelt. New Antibiotics. In: Pathogenomics – Genome analysis of pathogenic microbes. J. Hacker and U. Dobrindt (Ed.), Wiley VCH, 505-531 (2005).

Brötz-Oesterhelt, H., D. Beyer, H.-P. Kroll, R. Endermann, C. Ladel, W. Schroeder, B. Hinzen, S. Raddatz, H. Paulsen, K. Henninger, J. E. Bandow, H.-G. Sahl and H. Labischinski. Dysregulation of bacterial proteolytic machinery by a new class of antibiotics. Nature Medicine. 11, 1082-1087 (2005). ⇓

Freiberg, C. and H. Brötz-Oesterhelt. Functional genomics in antibacterial drug discovery. Drug Discov. Today. 10, 927-935 (2005). ⇓

Brötz-Oesterhelt H., J. E. Bandow and H. Labischinski. Bacterial proteomics and its role in antibacterial drug discovery. Mass. Spec. Rev. 24, 549-565 (2005). ⇓

2000 - 2004

Freiberg, C., H. Brötz-Oesterhelt and H. Labischinski. The impact of transcriptome and proteome analyses on antibiotic drug discovery. Curr. Opin. Microbiol. 7, 451–459 (2004). ⇓

J. Pohlmann and Brötz-Oesterhelt H. New aminoacyl-tRNA synthetase inhibitors as antibacterial agents. Current Drug Targets – Infectious Disorders 4, 261-272 (2004). ⇓

Brötz-Oesterhelt, H. Antibiotics -Actions, Origins, Resistance. Book review. Angew. Chem. Int. Ed. 43, 2879-2880 (2004).

Beyer, D., H. P. Kroll, R. Endermann, G. Schiffer, S. Siegel, M. Bauser, J. Pohlmann, M. Brands, K. Ziegelbauer, D. Häbich and H. Brötz-Oesterhelt. New class of phenylalanyl-tRNA synthetase inhibitors with high potency and broad spectrum activity. Antimicrob. Agents Chemother. 48, 525-532 (2004). ⇓

Brötz-Oesterhelt, H., I. Knezevic, S. Bartel, T. Lampe, U. Warnecke-Eberz, K. Ziegelbauer, D. Häbich and H. Labischinski. Specific and potent inhibition of NAD+-dependent DNA ligase by pyridochromanones. J. Biol. Chem. 278, 39435-39442 (2003). ⇓

Bandow, J. E., D. Becher, K. Büttner, F. Hochgräfe, C. Freiberg, H. Brötz and M. Hecker. The role of peptide deformylase in protein biosynthesis: A proteomic study. Proteomics, 3, 299-306 (2003). ⇓

Bandow, J. E., H. Brötz, L. I. O. Leichert, H. Labischinski and M. Hecker. A proteomic approach to understanding antibiotic action. Antimicrob. Agents Chemother., 47, 948-955 (2003). ⇓

Bandow, J. E., H. Brötz and M. Hecker: B. subtilis tolerance of moderate concentrations of rifampicin involves the sB-dependent general and multiple stress response. J. Bacteriol., 184, 459-467 (2002). ⇓

Freiberg, C., B. Wieland, F. Spaltmann, K. Ehlert, H. Brötz and H. Labischinski: Identification of novel essential Escherichia coli genes conserved among pathogenic bacteria. J. Mol. Microbiol. Biotechnol. 3, 483-489 (2001). ⇓

Brötz, H. and H.-G. Sahl: New insights into the mechanism of action of lantibiotics - diverse biological effects by binding to the same molecular target. J. Antibiot. Chemother. 46, 1-6 (2000). ⇓

1995 - 1999

Brötz, H., M. Josten, I. Wiedemann, U. Schneider, G. Bierbaum and H.-G. Sahl: Role of lipid-bound peptidoglycan precursors in the formation of pores by nisin, epidermin and other lantibiotics. Mol. Microbiol. 30, 317-328 (1998). ⇓

Brötz, H., G. Bierbaum, K. Leopold, P. E. Reynolds and H.-G. Sahl: The lantibiotic mersacidin inhibits peptidoglycan synthesis by targeting lipid II. Antimicrob. Agents Chemother., 42, 154-160 (1998). ⇓

Brötz, H., G. Bierbaum, P. E. Reynolds and H.-G. Sahl: The lantibiotic mersacidin inhibits peptidoglycan synthesis at the level of transglycosylation. Eur. J. Biochem., 246, 193-199 (1997). ⇓

Molitor E., C. Kluczny, H. Brötz, G. Bierbaum, R. Jack and H.-G. Sahl: Effects of the lantibiotic mersacidin on the morphology of staphylococci. Zbl. Bakt., 284, 318-328 (1996). ⇓

Brötz, H., G. Bierbaum and H.-G. Sahl: Modes of action of lantibiotics. In: Recent Research and Development in Antimicrobial Agents and Chemotherapy, G. Pandalai (Ed.), Research Signpost, Trivandrum, Vol. 1, 125-134 (1996).

Bierbaum, G., H. Brötz, H.-P. Koller and H.-G. Sahl: Cloning, sequencing and production of the lantibiotic mersacidin. FEMS Microbiol. Lett., 127, 121-126 (1995). ⇓

Brötz, H., G. Bierbaum, A. Markus, E. Molitor and H.-G. Sahl: Mode of action of the lantibiotic mersacidin: Inhibition of peptidoglycan biosynthesis via a novel mechanism? Antimicrob. Agents Chemother., 39, 714-719 (1995). ⇓

 

Patents

Klenke B, Wiegand I, Schiffer G, Brötz-Oesterhelt H, Maiti S, Khan J, Reddy A, Yang Z, Hena M, Jia G, Ligong O, Liang H, Yip J, Gao C, Tajammul S, Mohammad R, Biswajeet G. WO/2013/110643, PCT/EP2013/051217. Amidin substituted beta-lactam compounds, their preparation and use as antibacterial agents

Lerchen, H.-G., G. Schiffer, H. Brötz-Oesterhelt, A. Mayer-Bartschmid, S. Eckermann, C. Freiberg und D. Häbich. WO 2007/059908. Novel cyclic iminopeptide derivatives and a process for preparing cyclic iminopeptide derivatives.

Lerchen, H.-G., G. Schiffer, H. Brötz-Oesterhelt, A. Mayer-Bartschmid, S. Eckermann, C. Freiberg, R. Endermann, J. Schuhmacher, H. Meier, N. Svenstrup, S. Seip, M. Gehling und D. Häbich. WO 2006/103010. Cyclic iminopeptide derivatives.

Hinzen, B., H. Brötz, R. Endermann, K. Henninger, H. Paulsen, S. Raddatz und S. Anlauf. DE 10308107. Mono-ungesättigte Acyl-Verbindungen.

Hinzen, B., H. Brötz, R. Endermann, K. Henninger, H. Paulsen, S. Raddatz, T. Lampe, V. Hellwig, and A. Schumacher. DE 10219225. Serinderivate.

Bauser, M., D. Beyer, H. Brötz, R. Endermann, M. Hauswald, H.-P. Kroll, J. Pohlmann, S. Siegel, G. Schiffer. DE 10155684. Thiazolharnstoffe.

Hinzen, B., H. Brötz, R. Endermann, K. Henninger, H. Paulsen, S. Raddatz, T. Lampe, V. Hellwig, A. Schumacher. WO-03024996-A2 Antibacterial macrocycles.

Brötz, H., K. Ehlert, C. Freiberg, F. Spaltmann, B. Wieland und H. Labischinski. WO-0/61792: Novel essentiell bacterial genes and their proteins.