Certain Staphylococcus species are part of the normal microbiota of human skin and probably even of the intestine. We are studying the physiology of staphylococci and their mutual communication with the host and microbiota. We investigate how commensal bacteria overcome the immunological barriers of the skin, how they can survive in the host, and how they control human cell's proliferation and motility. Their influence on skin even goes so far that they are able to accelerate wound healing through their secreted effectors. This was also one of the recent breakthroughs in our research, namely, to demonstrate that many members of the skin microbiota and even gut microbiota secrete neurotransmitters that interact with adrenergic receptors of host cells to interfere with their metabolism.
Over the past 10 years, we have identified and isolated a number of bacterial effectors and studied their effect on bacteria and host cells.
Some recent publications:
Bacterial lipoproteins (Lpp) and immune stimulation and immune evasion
Nguyen, M.T., and Götz, F. (2016) Lipoproteins of Gram-Positive Bacteria: Key Players in the Immune Response and Virulence. Microbiol Mol Biol Rev 80: 891-903.
Nguyen, M.T., Kraft, B., Yu, W., Demicrioglu, D.D., Hertlein, T., Burian, M., Schmaler, M., Boller, K., Bekeredjian-Ding, I., Ohlsen, K., Schittek, B., and Götz, F. (2015) The νSaα Specific Lipoprotein Like Cluster (lpl) of S. aureus USA300 Contributes to Immune Stimulation and Invasion in Human Cells. PLoS Pathog 11: e1004984.
Nguyen, M.T., Uebele, J., Kumari, N., Nakayama, H., Peter, L., Ticha, O., Woischnig, A.K., Schmaler, M., Khanna, N., Dohmae, N., Lee, B.L., Bekeredjian-Ding, I., and Götz, F. (2017) Lipid moieties on lipoproteins of commensal and non-commensal staphylococci induce differential immune responses. Nat Commun 8: 2246.
Nguyen, M.T., Matsuo, M., Niemann, S., Herrmann, M., and Götz, F. (2020) Lipoproteins in Gram-Positive Bacteria: Abundance, Function, Fitness. Front Microbiol 11: 582582.
Tribelli, P.M., Luqman, A., Nguyen, M.T., Madlung, J., Fan, S.H., Macek, B., Sass, P., Bitschar, K., Schittek, B., Kretschmer, D., and Götz, F. (2019) Staphylococcus aureus Lpl protein triggers human host cell invasion via activation of Hsp90 receptor. Cell Microbiol: e13111.
Bacterial neurotransmitters and interference with host neuro receptors
Luqman, A., Ebner, P., Reichert, S., Sass, P., Kabagema-Bilan, C., Heilmann, C., Ruth, P., and Götz, F. (2019) A new host cell internalisation pathway for SadA-expressing staphylococci triggered by excreted neurochemicals. Cell Microbiol: e13044.
Luqman, A., Kharisma, V.D., Ruiz, R.A., and Götz, F. (2020a) In Silico and in Vitro Study of Trace Amines (TA) and Dopamine (DOP) Interaction with Human Alpha 1-Adrenergic Receptor and the Bacterial Adrenergic Receptor QseC. Cell Physiol Biochem 54: 888-898.
Luqman, A., Muttaqin, M.Z., Yulaipi, S., Ebner, P., Matsuo, M., Zabel, S., Tribelli, P.M., Nieselt, K., Hidayati, D., and Götz, F. (2020b) Trace amines produced by skin bacteria accelerate wound healing in mice. Commun Biol 3: 277.
Luqman, A., Nega, M., Nguyen, M.T., Ebner, P., and Götz, F. (2018) SadA-Expressing Staphylococci in the Human Gut Show Increased Cell Adherence and Internalization. Cell Rep 22: 535-545.
The human skin microbiota harbors neurotransmitter-producing bacteria
Luqman, A., Zabel, S., Rahmdel, S., Merz, B., Gruenheit, N., Harter, J., Nieselt, K., and Götz, F. (2020) The Neuromodulator-Encoding sadA Gene Is Widely Distributed in the Human Skin Microbiome. Front Microbiol 11: 573679.
New plant-derived antibiotics and their mode of action
Guttroff, C., Baykal, A., Wang, H., Popella, P., Kraus, F., Biber, N., Krauss, S., Götz, F., and Plietker, B. (2017) Polycyclic Polyprenylated Acylphloroglucinols: An Emerging Class of Non-Peptide-Based MRSA- and VRE-Active Antibiotics. Angew Chem Int Ed Engl 56: DOI: 10.1002/anie.201707069.
Saising, J., Nguyen, M.T., Härtner, T., Ebner, P., Al Mamun Bhuyan, A., Berscheid, A., Muehlenkamp, M., Schakermann, S., Kumari, N., Maier, M.E., Voravuthikunchai, S.P., Bandow, J., Lang, F., Brötz-Oesterhelt, H., and Götz, F. (2018) Rhodomyrtone (Rom) is a membrane-active compound. Biochim Biophys Acta 1860: 1114-1124.
Wang, H., Kraus, F., Popella, P., Baykal, A., Guttroff, C., Francois, P., Sass, P., Plietker, B., and Götz, F. (2019) The Polycyclic Polyprenylated Acylphloroglucinol Antibiotic PPAP 23 Targets the Membrane and Iron Metabolism in Staphylococcus aureus. Front Microbiol 10: 14.
Unraveling new mechanism in staphylococcal cell separation by the major autolysin (Atl)
Nega, M., Tribelli, P.M., Hipp, K., Stahl, M., and Götz, F. (2020) New insights in the coordinated amidase and glucosaminidase activity of the major autolysin (Atl) in Staphylococcus aureus. Commun Biol 3: 695
Discovery of a new CO2/bicarbonate transporter in Firmicutes
Fan, S.H., Ebner, P., Reichert, S., Hertlein, T., Zabel, S., Lankapalli, A.K., Nieselt, K., Ohlsen, K., and Götz, F. (2019) MpsAB is important for Staphylococcus aureus virulence and growth at atmospheric CO2 levels. Nat Commun 10: 3627.