Ecology of staphylococcal nasal colonization (Bernhard Krismer et al): Colonization of the human nose by S. aureus in one third of the human population represents a major risk factor for invasive infections. The basis for adaptation of S. aureus to this specific habitat and reasons for the human predisposition to become colonized have remained largely unknown. We found that metabolite concentrations are similar in S. aureus carriers and non-carriers indicating that the predisposition to be a carrier is not associated with differences in nasal nutrient supply. Based on nasal metabolite profiles a synthetic nasal medium was composed representing a suitable surrogate environment for in vitro simulation of nasal colonization and gene expression. Notably, methionine biosynthesis was found to represents a promising new target for anti-staphylococcal agents because expression of a central S. aureus methionine-biosynthetic enzyme was strongly upregulated during nasal colonization and a specific enzyme inhibitor exhibited anti-staphylococcal activity.
Bernhard Krismer studied Biology in Innsbruck, Austria, and Tübingen, Germany, and obtained Diploma and PhD degrees in Microbiology. He held a postdoctoral position in the lab of Friedrich Götz in Tübingen. In 2002 he joined the company DR. PETRY genmedics GmbH as CSO (chief scientific officer), being responsible for contract research, development of large scale protein expression and purification with a proprietary expression system, and screening of natural product libraries. Since 2008 he is a member of the group of Prof. Andreas Peschel focusing on the human nose as habitat for pathogenic S. aureus and the development of new antiinfectives.
DFG - Research Training Site GRK 1708 - Bacterial survival strategies
Project: Staphylococcal resistance to antimicrobial fatty acids
Research area ‘hospital-associated and antibiotic-resistant bacterial infections’. Projekt: New strategies for specific decolonization of S. aureus