The light and electron microscopy service and research unit provides preparative techniques for morphological studies as well as for immunolocalisation of gene products. The focus of our methodological research is on cryoimmobilisation-based specimen preparation procedures (e.g., high pressure freezing and freeze-substitution) for improved preservation of structural integrity and antigenicity as well as on improving methods for detection of low copy number antigens. This methodological approach allows a detailed molecular analysis of cellular processes in the context of complex biological systems.
Techniques provided include negative staining, glycerol spraying, freeze-drying, cryoimmobilisation, freeze-substitution, microwave fixation and resin embedding, conventional resin embedding, low temperature embedding (methacrylates), ultrathin Tokuyasu cryosectioning & immunogold labelling, resin section immunogold labelling, pre-embedding immunogold labelling, critical-point drying, sputter coating, conventional and confocal light microscopy.
| Dr. Sandra Richter | Head of light- and electron microscopy unit |
Technician |
Veranstalter: Dr. Katharina Hipp (Max-Planck-Institut für Entwicklungsbiologie), Dr. Sandra Richter (ZMBP), Dr. Stefan Fischer (Geomikrobiologie)
Termin: jedes WS, ganztägiger 10-tägiger Ferienkurs
Weitere Auskünfte: siehe ALMA
katharina.hipp(a)tuebingen.mpg.de (Tel: 07071-601310) oder sandra.richter(a)zmbp.uni-tuebingen.de (Tel: 07071-2976662)
Inhalt:
Termin: jedes SoSe
Dr. Sandra Richter (ZMBP), Dr. Martin Beyer
weitere Auskünfte: Dr. Sandra Richter
Tel: 29-76662
email: sandra.richter(a)zmbp.uni-tuebingen.de
Inhalt: Negativkontrastierung, Schwermetall-Beschichtungstechniken, Glyzerin-Sprühtechnik, Cryofixierung, gefroren-hydratisierte Präparate, TEM.
Termin: SS und WS, jeweils 2 Vormittage
Ort: Auf der Morgenstelle 32, EM-Labor, Ebene 3
Dozenten: Dr. Sandra Richter
weitere Auskünfte und Anmeldung: siehe ALMA
Inhalt: Theorie und Praxis der Epi-Fluoreszenz- und konfokalen Lichtmikroskopie mit Übungen am Leica SP8 und/oder Zeiss LSM 880
Dauer: etwa 4 Stunden (Voraussetzung für selbständiges Arbeiten am Gerät)
Termin: nach Absprache
Ort: Auf der Morgenstelle 32, Mikroskopie, Ebene 3, Raum 3U01, 3R01 oder 3V01
weitere Auskünfte und Anmeldung: Dr. Sandra Richter sandra.richter(a)zmbp.uni-tuebingen.de
Veranstalter: Dr. Heinz Schwarz und Dr. Katharina Hipp (Max-Planck-Institut für Entwicklungsbiologie), Dr. Sandra Richter (ZMBP) in Zusammenarbeit mit Dr. Bruno Humbel und Dr. York-Dieter Stierhof mit Unterstützung der Deutschen Gesellschaft für Elektronenmikroskopie.
Termin: im Herbst jedes Jahres
Weitere Auskünfte: Katharina Hipp (katharina.hipp(a)tuebingen.mpg.de) oder sandra.richter(a)zmbp.uni-tuebingen.de
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