Center for Plant Molecular Biology

Research Group Timmermans

Pattern formation and cell differentiation during organogenesis



Ma X., Denyer T., Timmermans M. C. P. (2020). PscB: A Browser to Explore Plant Single Cell RNA-Sequencing Datasets. Plant Physiologydoi

Klesen S., Hill K., Timmermans M. C. P. (2020). Small RNAs as plant morphogens. Current Topics in Developmental Biologydoi


Knauer S., Javelle M., Li L., Li X., Ma X., Wimalanathan K., ... & Timmermans M. C. P. (2019). A high-resolution gene expression atlas links dedicated meristem genes to key architectural traits. Genome researchdoi

Denyer T., Ma X., Klesen S., Scacchi E., Nieselt K., Timmermans M.C.P., (2019) Spatiotemporal Developmental Trajectories in the Arabidopsis Root Revealed Using High-Throughput Single-Cell RNA Sequencing. Dev Cell, 48(6), 840-852. doi


Skopelitis, D.S., Hill, K., Klesen, S., Marco, C.F., von Born, P., Chitwood, D.H., Timmermans, M.C.P., (2018) Gating of miRNA movement at defined cell-cell interfaces governs their impact as positional signals. Nature Comm. 9, 3107. doi


Skopelitis, D.S., Benkovics, A.H., Husbands, A.Y. and Timmermans, M.C.P., (2017) Boundary formation through a direct threshold-based readout of mobile small RNA gradients. Dev cell, 43(3), 265-273. doi


Plavskin, Y., Nagashima, A., Perroud, P.-F., Hasebe, M., Quatrano, R.S., Atwal, G.S., Timmermans M.C.P. (2016) Ancient trans-acting siRNAs confer robustness and sensitivity onto the auxin response. Dev Cell 36, 276-289.


Husbands, A.Y., Benkovics, A.H., Nogueira, F.T.S., Lodha, M., and Timmermans, M.C.P. (2015) The ASYMMETRIC LEAVES complex employs multiple modes of regulation to affect adaxial-abaxial patterning and leaf complexity. Plant Cell 27, 3321-3335.

Leiboff, S., Li, X., Hu, A., Todt, N., Yang, J., Li, X., Yu, X., Muehlbauer, G., Timmermans, M.C.P., Yu, J., Schnable, P.S., and Scanlon, M.J. (2015) Genetic control of morphometric diversity in the maize shoot apical meristem. Nature Comm. 6, 8974.

Petsch, K.A., Manzotti, P.S., Tam, O.H., Meeley, R., Hammell, M., Consonni, G., and Timmermans M.C.P. (2015) Novel DICER-LIKE1 siRNAs bypass the requirement for DICER-LIKE4 in maize development. Plant Cell 27, 2163-2177.

Thompson, A.M., Yu, J., Timmermans, M.C., Schnable, P., Crants, J.C., Scanlon, M.J., and Muehlbauer, G.J. (2015) Diversity of maize shoot apical meristem architecture and its relationship to plant morphology. G3 5, 819-827.


Dotto, M.C., Petsch, K.A., Aukerman, M.J., Beatty, M., Hammell, M., and Timmermans, M.C.P. (2014) Genome-wide analysis of leafbladeless1-regulated and phased small RNAs underscores the importance of the TAS3 ta-siRNA pathway to maize development. PLoS Genet. 10, e1004826.

Benkovics, A.H. and Timmermans, M.C.P. (2014) Developmental patterning by gradients of mobile small RNAs. Curr Opin Genet Dev. 27, 83-91.

Thompson, A.M., Crants, J.E., Schnable, P.S., Yu, J., Timmermans, M.C.P., Springer, N.M., Scanlon, M.J., and Muehlbauer, G.J. (2014) Genetic control of maize shoot apical meristem architecture. G3 4, 1327-1337.

Li L, Eichten SR, Shimizu R, Petsch K, Yeh CT, Wu W, Chettoor AM, Givan SA, Cole RA, Fowler JE, Evans MM, Scanlon MJ, Yu J, Schnable PS, Timmermans MCP, Springer NM, Muehlbauer GJ. (2014) Genome-wide discovery and characterization of maize long non-coding RNAs. Genome Biol. 15, R40.


Lodha, M., Marco, C.F., and Timmermans, M.C.P. (2013) The ASYMMETRIC LEAVES complex maintains repression of KNOX homeobox genes via direct recruitment of Polycomb-repressive complex2. Genes & Dev. 27, 596-601.

Zhai, J., Zhao, Y., Simon, S.A., Huang, S., Petsch, K., Arikit, S., Pillay, M., Ji, L., Xie, M., Cao, X., Yu, B., Timmermans, M., Yang, B., Chen, X., and Meyers, B.C. (2013) Plant microRNAs display differential 3' truncation and tailing modifications that are ARGONAUTE1 dependent and conserved across species. Plant Cell 25, 2417-2428.

Li, L., Petsch, K., Shimizu, R., Liu, S., Xu, W.W., Ying, K., Yu, J., Scanlon, M.J., Schnable, P.S., Timmermans, M.C., Springer, N.M., and Muehlbauer, G.J. (2013) Mendelian and non-Mendelian regulation of gene expression in maize. PLoS Genet. 9, e1003202.

Knauer, S., Holt, A.L., Rubio-Somoza, I., Tucker, E.J., Hinze, A., Pisch, M., Javelle, M., Timmermans, M.C.P., Tucker, M.R., and Laux, T. (2013) A protodermal miR394 signal defines a region of stem cell competence in the Arabidopsis shoot meristem. Dev. Cell 24, 125-132.


Plavskin, Y., and Timmermans, M.C.P. (2012) Small RNA-Regulated Networks and the Evolution of Novel Structures in Plants. CSH Symp Quant Biol. 77, 221-233.

Takacs, E.M., Li, J., Du, C., Ponnala, L., Janick-Buckner, D., Yu, J., Muehlbauer, G.J., Schnable, P.S., Timmermans, M.C.P., Sun, Q., Nettleton, D., and Scanlon, M.J. (2012) Ontogeny of the maize shoot apical meristem. Plant Cell 24, 3219-3234.

Li, X., Zhu, C., Yeh, C.T., Wu, W., Takacs, E., Petsch, K., Tian, F., Bai, G., Buckler, E., Muehlbauer, G., Timmermans, M.C.P., Scanlon, M., Schnable, P., and Yu, J. (2012) Genic and non-genic contributions to natural variation of quantitative traits in maize. Genome Res. 22, 2436-2444.

Javelle, M., and Timmermans, M.C.P. (2012) In situ localization of small RNAs in plants using LNA probes. Nature Prot. 7, 533-541.

Skopelitis, D.S., Husbands, A.Y., and Timmermans, M.C.P. (2012) Plant small RNAs as morphogens. Curr. Opinion Cell Biol. 24, 217-224.


Chitwood, D.H. and Timmermans, M.C.P. (2010) Small RNAs are on the move. Nature 467, 415-419.

Douglas, R.N., Wiley, D., Sarkar, A., Springer, N., Timmermans, M.C.P., and Scanlon, M.J. (2010) ragged seedling2 encodes an ARGONAUTE7-like protein required for mediolateral expansion, but not dorsiventrality, of maize leaves. Plant Cell 22, 1441-1451.

Husbands, A.Y., Chitwood, D.H., Plavskin, Y. and Timmermans, M.C.P. (2009) Signals and prepatterns: new insights into organ polarity in plants. Genes & Dev. 23, 1986-1997.

Chitwood, D.H., Nogueira F.T.S., Howell, M., Montgomery, T.A. Carrington, J.C. and Timmermans, M.C.P. (2009) Pattern formation via small RNA mobility. Genes & Dev. 23, 549-554.

Nogueira, F.T.S., Chitwood, D.H., Madi, S., Kazuhiro Ohtsu, K., Schnable, P.S., Scanlon, M.J., and Timmermans, M.C.P. (2009) Regulation of small RNA accumulation in the maize shoot apex. PLoS Genetics 5, e1000320.

Guo, M., Thomas, J., Collins, G., and Timmermans, M.C.P. (2008) Direct repression of KNOX loci by the ASYMMETRIC LEAVES1 complex of Arabidopsis. Plant Cell 20, 48-58.

Nogueira, F.T.S., Madi, S., Chitwood, D.H., Juarez, M.T., and Timmermans, M.C.P. (2007) Two small regulatory RNAs establish opposing fates of a developmental axis. Genes & Dev. 21, 750-755.

Chitwood, D.H., Guo, M., Nogueira, F.T.S., and Timmermans, M.C.P. (2007) Establishing leaf polarity: the role of small RNAs and positional signals in the shoot apex. Development 134, 813-823.

Phelps-Durr, T.L., Thomas, J., Vahab, P., and Timmermans, M.C.P. (2005) Maize rough sheath2 and its Arabidopsis orthologue ASYMMETRIC LEAVES1 interact with HIRA, a predicted histone chaperone, to maintain knox gene silencing and determinacy during organogenesis. Plant Cell 17, 2886-2898.

Juarez, M.T., Twigg, R., and Timmermans, M.C.P. (2004) Specification of adaxial cell fate during maize leaf development. Development 131, 4533-4544.

Juarez, M.T., Kui, J.S., Thomas, J., Heller, B.A., and Timmermans, M.C.P. (2004) microRNA mediated repression of rolled leaf1 specifies maize leaf polarity. Nature 428, 84-88.

Kim, M., McCormick, S., Timmermans, M., and Sinha, N. (2003) The expression domain of PHANTASTICA determines leaflet placement in compound leaves. Nature 424, 438-443.

Timmermans, M.C.P., Hudson, A., Becraft, P.W., and Nelson, T. (1999) ROUGH SHEATH2: a Myb domain protein that represses knox homeobox genes in maize lateral organ primordia. Science 284, 151-153.

Timmermans, M.C.P., Schultes, N.P., Jankovsky, J.P., and Nelson, T. (1998) Leafbladeless1 is required for dorsoventrality of lateral organs in maize. Development 125, 2813-2823.