Prof. Dr. Erik Schäffer
Universität Tübingen
ZMBP, Cellular Nanoscience
Auf der Morgenstelle 32, 72076 Tübingen
+49 7071 - 29 72076
Fax +49 7071 - 29 5042
erik.schaefferspam prevention@zmbp.uni-tuebingen.de
Die spezifischen kinetischen und mechanischen Eigenschaften von Phragmoplast-Orienting-Kinesin (POK) und Kinesin-8-Motoren und Wechselwirkungspartnern werden mit Einzelmolekülfluoreszenz-mikroskopie und optischen Pinzetten Messungen von aufgereinigten rekombinanten Proteinen charakterisiert. Um die Bündelung von Mikrotubuli und Bindung an die Zellmembran zu untersuchen, werden wir einen minimalen in vitro Assay für die kortikale Zellteilungsstelle rekonstituieren, der aus artifiziellen Membransystemen, POKs, ihren Interaktoren und Mikrotubuli besteht. Langfristig werden wir den molekularen Mechanismus, wie die Zellteilungsebene positioniert wird, aufklären.
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