Transfection Service 

We provide the protoplasts and the reagents, You perform the transfection. 
Our cells and protocol are streamlined so that any researcher at any education/experience level can easily learn how to do it.  You can order Tubes of Protoplasts in W5 for Tuesday morning or Protoplasts in Ca2+M digestion buffer for Thursday afternoon.

These can be booked using the web form BOOKING or by sending an email directly to Transformation. Please book at least three working days in advance as the cells need to be sub-cultured beforehand, otherwise we cannot ensure that the cell will be available.

1.  To order: just phone or email Transformation at least 3 days in advance (73223). We are redesigning our web-ordering page in the meantime, but it would work as so:

   1. Please choose the week
   2. Type in your Name, Phone Number and Research Group
   3. Book number of 10 mL Tubes you think you will need
      ONLY NUMBER of TUBES you need,
      then press “submit”.
   4. If you need to cancel, check the box you want to remove and submit.
      Please delete only your reservation!
   5. All actions are logged in a file (history).

    

2. Notes: On Tuesday, you can get protoplasts in W5 ready for rebuffering in MM. On Thursday, you can get protoplasts digesting in Ca2+M and just need to be washed,  resuspended in W5, and then rebuffered in MM.  All of the solutions are provided by us, so you only need to bring your DNA and do the transefction. 1 Tube (10mL) is usually this is enough for about 30 transfections (Large Scale) or more (Mid and Small scale 2mL eppis, 96-well plates) depending on your assay. If it is your first time doing so, then it is best to transfect them along with us. For mid and high-throughput assays contact Kenneth. We will show you how to do it and how to fill out our invoice/tracking sheets. After that, you can pick up the protoplasts and any solutions you need whenever you want.

UPDATE: 10 Sep 2025

Transfection Protocol can be performed at various scales:
Protoplasts in W5 at 4°C. These protoplasts ordered by the tube are ready to be placed in the transfection medium (~5mL in MM once concentrated) by you when you are ready and are good for up to 16 hrs at 4°C. That means you can do your transfections on Tuesday morning, Thursday evening or even Friday morning! We provide all the solutions and tubes.

• Large Scale - 120µL reactions
  5 - 40 µg DNA per transfection in 80 µL H₂O
  14ml round-bottom PP tubes. Circa 420.000 protos/transf.
• Mid scale - 60 µL reactions
  2 - 20 µg DNA per transfection in 40 µL H₂O
  2mL Eppendorf, aseptic (on bench) or 96-well plates. Circa 210.000 protos/transf.
• Small scale - 30 µL reactions
  up to 12 per transfection (aseptic) in 20 µL H₂O
  2mL Eppendorf, aseptic (on bench) or 96-well plates. Circa 105.000 protos/transf.
• 96-well transfections are performed with the Liquidator96-well pipette. Both 30µL and 60µL transfections can be effectively performed in a 96-well deepwell format.

Please ask for assistance the first time doing any experiments.