Research Group Lahaye

2022

Schenstnyi K, Strauß A, Dressel A, Morbitzer R, Wunderlich M, Andrade AG, Phan TTT, Aguilera, de los Angeles P, Brancato C, Berendzen KW, and Lahaye T (2022).
The tomato resistance gene Bs4 suppresses leaf watersoaking phenotypes induced by AvrHah1, a transcription activator-like effector from tomato-pathogenic xanthomonads.
New Phytologist. https://doi.org/10.1111/nph.18456

Forner J, Kleinschmidt D, Meyer EH, Fischer A, Morbitzer R, Lahaye T, Schöttler MA, Bock R. (2022)
Targeted introduction of heritable point mutations into the plant mitochondrial genome.
Nature Plants 8: 245–256.

Nowack MK, Holmes DR, Lahaye T. (2022)
TALE-induced cell death executors: an origin outside immunity?
Trends in Plant Science 27: 536-548.

2021

Krönauer C, Lahaye T. (2021).
The flavin monooxygenase Bs3 triggers cell death in plants, impairs growth in yeast and produces H2O2 in vitro. PLoS One 16: e0256217.

Lundberg DS, Ayutthaya PPN, Strauss A, Shirsekar G, Lo WS, Lahaye T, Weigel D. (2021).
Host-associated microbe PCR (hamPCR) enables convenient measurement of both microbial load and community composition. eLife 10.

2020

Holmes DR, Morbitzer R, Wunderlich M, Sun H, El Kasmi F, Schneeberger K, Lahaye T (2020).
A shortcut in forward genetics: concurrent discovery of mutant phenotype and causal mutation in Arabidopsis M2 families via MAD-mapping.
bioRxiv: DOI: 10.1101/2020.06.29.177808.

Zhou F, Emonet A, Tendon VD, Marhavy P, Wu D, Lahaye T, and Geldner N (2020).
Co-incidence of damage and microbial patterns controls localized immune responses in roots.
Cell: 180, 440-453.

2019

Krönauer C, Kilian J, Strauß T, Stahl M, and Lahaye T (2019).
Cell death triggered by the YUCCA-like Bs3 protein coincides with accumulation of salicylic acid and pipecolic acid but not of indole-3-acetic acid.
Plant Physiol: 180, 1647-1659.

Wu D, von Roepenack-Lahaye, E, Buntru M, de Lange O, Schandry N, Pérez-Quintero AL, Weinberg Z, Lowe-Power TM, Szurek B, Michael AJ, Allen, C, Schillberg S, and Lahaye T (2019).
A plant pathogen type III effector protein subverts translational regulation to boost host polyamine levels.
Cell Host Microbe: 26, 638-649.

Luo M, Li H, Chakraborty S, Morbitzer R, Rinaldo A, Upadhyaya N, Bhatt D, Louis S, Richardson T, Lahaye T, and Ayliffe M. (2019).
Efficient TALEN-mediated gene editing in wheat.
Plant Biotechnol J: 17, 2026-2028

2018

Wu D, Schandry N and Lahaye T (2018).
A modular toolbox for Golden-Gate-based plasmid assembly streamlines the generation of Ralstonia solanacearum species complex knockout strains and multi-cassette complementation constructs.
Mol. Plant Pathol.: 19, 1511-1522.

Lowe-Power TM, Hendrich CG, von Roepenack-Lahaye E, Li B, Wu D, Mitra R, Dalsing BL, Ricca P, Naidoo J, Cook D, Jancewicz A, Masson P, Thomma B, Lahaye T, Michael AJ, and Allen C (2018).
Metabolomics of tomato xylem sap during bacterial wilt reveals Ralstonia solanacearum produces abundant putrescine, a metabolite that accelerates wilt disease.
Environ Microbiol: 20, 1330–1349.

Gochez AM, Huguet-Tapia JC, Minsavage GV, Shantaraj D, Jalan N, Strauss A, Lahaye T, Wang N, Canteros BI, Jones JB, and Potnis, N (2018).
Pacbio sequencing of copper-tolerant Xanthomonas citri reveals presence of a chimeric plasmid structure and provides insights into reassortment and shuffling of transcription activator-like effectors among X. citri strains.
BMC Genomics: 19, 16

El Kasmi F, Horvath D, and Lahaye T (2018).
Microbial effectors and the role of water and sugar in the infection battle ground.
Curr Opin Plant Biol, 44, 98-107

2017

de Lange O, Schandry N, Wunderlich M, Berendzen KW, Lahaye T (2017)
Exploiting the sequence diversity of TALE-like repeats to vary the strength of dTALE-promoter interactions.
Synthetic Biology, 2

Schwartz AR, Morbitzer R, Lahaye T, Staskawicz BJ (2017)
TALE-induced bHLH transcription factors that activate a pectate lyase contribute to water soaking in bacterial spot of tomato.
Proc Natl Acad Sci USA, 114, E897-E903

Shantharaj D, Römer P, Figueiredo JFL, Minsavage GV, Krönauer C, Stall RE, Moore GA, Fisher LC, Hu Y, Horvath DM, Lahaye T, Jones JB (2017)
An engineered promoter driving expression of a microbial avirulence gene confers recognition of TAL effectors and reduces growth of diverse Xanthomonas strains in citrus.
Mol Plant Pathol, 18, 976-989

2016

Budhagatapalli N, Schedel S, Gurushidze M, Pencs S, Hiekel S, Rutten T, Kusch S, Morbitzer R, Lahaye T, Panstruga R, Kumlehn J, Hensel G (2016)
A simple test for the cleavage activity of customized endonucleases in plants.
Plant Methods, 12, 18.

Denancé N, Lahaye T, Noël LD (2016)
Editorial: genomics and effectomics of the crop killer Xanthomonas.
Front Plant Sci, 7, 71

Schandry N, de Lange O, Prior P, Lahaye T (2016)
TALE-like effectors are an ancestral feature of the Ralstonia solanacearum species complex and converge in DNA targeting specificity.
Front Plant Sci, 7.

2015

Cohn, M, Morbitzer R, Lahaye T, Staskawicz BJ (2015)
Comparison of gene activation by two TAL effectors from Xanthomonas axonopodis pv. manihotis reveals candidate host susceptibility genes in cassava.
Mol Plant Pathol, 17, 875-889

de Lange O, Wolf C, Thiel P, Krüger J, Kleusch C, Kohlbacher O, Lahaye, T (2015)
DNA-binding proteins from marine bacteria expand the known sequence diversity of TALE-like repeats.
Nucl Acids Res 43, 10065-10080

Müller TM, Böttcher C, Morbitzer R, Götz CC, Lehmann J, Lahaye T, Glawischnig E (2015)
TALEN-mediated generation and metabolic analysis of camalexin-deficient cyp71a12 cyp71a13 double knockout lines.
Plant Physiol, 168, 849-858.

2014

Boch J, Bonas U, Lahaye T (2014)
TAL effectors - pathogen strategies and plant resistance engineering.
New Phytol, 204, 823-832

Cohn M, Bart R, Shybut M, Dahlbeck D, Gomez M, Morbitzer R, Hou B-H, Frommer W, Lahaye T, Staskawicz B (2014)
Xanthomonas axonopodis virulence is promoted by a transcription activator like (TAL) effector mediated induction of a SWEET sugar transporter in cassava.
Mol Plant-Microbe Interact, 27, 1186-1198

De Lange O, Wolf C, Dietze J, Elsaesser J, Morbitzer R, Lahaye T. (2014)
Programmable DNA-binding proteins from Burkholderia provide a fresh perspective on the TALE-like repeat domain.
Nucleic Acids Res, 42, 7436-7449

Mussolino C, Alzubi J, Fine E, Morbitzer R, Cradick T, Lahaye T, Bao G, Cathomen T (2014)
TALENs facilitate targeted genome editing in human cells with high specificity and low cytotoxicity.
Nucleic Acids Res, 42, 6762-6773

De Lange O, Binder A, Lahaye T (2014)
From dead leaf, to new life: TAL effectors as tools for synthetic biology.
Plant J, 78, 753–771

Thanisch K, Schneider K, Morbitzer R, Solovei I, Lahaye T, Bultmann S, Leonhardt H (2014)
Targeting and tracing of specific DNA sequences with dTALEs in living cells.
Nucleic Acids Res, 42, e38

Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske M (2014)
A modular plasmid assembly kit for multigene expression, gene silencing and silencing rescue in plants.
PLoS ONE, 9, e88218
 

2013

Yanik M, Alzubi J, Lahaye T, Cathomen T, Pingoud A, Wende W (2013)
TALE-PvuII fusion proteins – novel tools for gene targeting.
PLoS ONE 8, e82539

Strauß, A, and Lahaye, T (2013)

Zinc fingers, TAL effectors or Cas9-based DNA binding proteins: what’s best for targeting desired genome loci?

Mol Plant, 6, 1384-1387


De Lange O, Schreiber T, Schandry N, Radeck J, Braun K-H, Koszinowski J, Heuer H, Strauß A, Lahaye T (2013)
Breaking the DNA binding code of Ralstonia solanacearum TAL effectors provides new possibilities to generate plant resistance genes against bacterial wilt disease. 

New Phytol, 199, 773-786

Meckler JF, Bhakta MS, Kim M-S, Ovadia R, Habrian CH, Zykovich A, Yu A, Lockwood SH, Morbitzer R, Elsäesser J, Lahaye T, Segal DJ, Baldwin EP (2013)
Quantitative analysis of TALE–DNA interactions suggests polarity effects
.
Nucl Acids Res, 41, 4118-4128
 

2012

Strauß T, Van Poecke R, Strauß A, Römer P, Minsavage GV, Singh S, Wolf C, Strauß A, Kim S, Lee HA, Yeom SI, Parniske M, Stall RE, Jones JB, Choi D, Prins M and Lahaye T (2012)
RNA-seq pinpoints a Xanthomonas TAL-effector activated resistance gene in a large crop genome.
Proc Natl Acad Sci USA, 109, 19480-19485

Bultmann S, Morbitzer R, Schmidt C, Thanisch K, Spada F, Elsaesser J, Lahaye T and Leonhardt H (2012)
Targeted transcriptional activation of silent oct4 pluripotency gene by combining designer TALEs and inhibition of epigenetic modifiers.
Nucl Acids Res, 40, 5368-5377
 

2011

Mussolino C, Morbitzer R, Lütge F, Dannemann N, Lahaye T and Cathomen T (2011)
A novel TALE nuclease scaffold enables high genome editing activity in combination with low toxicity.

Nucl Acids Res, 39, 9283-9293

Morbitzer R, Elsaesser J, Hausner J and Lahaye T (2011)
Assembly of custom TALE-type DNA binding domains by modular cloning.
Nucl Acids Res, 39, 5790-5799

Figueiredo JFL , Römer P, Lahaye T, Graham JH, White FF and Jones JB (2011)
Agrobacterium-mediated transient expression in citrus leaves: a rapid tool for gene expression and functional gene assay.
Plant Cell Rep, 30, 1339-1345
 

2010

Morbitzer R, Römer P, Boch J and Lahaye T (2010)
Regulation of selected genome loci using de novo engineered transcription activator-like effector (TALE)-type transcription factors.
Proc Natl Acad Sci USA, 107, 21617-21622

Bogdanove AJ, Schornack S and Lahaye T (2010)
TAL effectors: finding plant genes for disease and defense.
Curr Opin Plant Biol, 13, 394-401

Römer P, Recht S, Strauß T, Elsaesser J, Schornack S, Boch J, Wang S, and Lahaye T (2010)
Promoter elements of rice susceptibility genes are bound and activated by specific TAL effectors from the bacterial blight pathogen, Xanthomonas oryzae pv. oryzae.
New Phytol, 187, 1048-1057

Römer P, Jordan T and Lahaye T (2010)
Identification and application of a DNA-based marker that is diagnostic for the pepper (Capsicum annuum) bacterial spot resistance gene Bs3.
Plant Breed, 129, 737-740
 

2009

Boch J, Scholze H, Schornack S, Landgraf A, Hahn S, Kay S, Lahaye T, Nickstadt A, and Bonas U (2009)
Breaking the code of DNA-binding specificity of TAL-type III effectors.
Science, 326, 1509-1512

Römer P, Recht S and Lahaye T (2009)
A single plant resistance gene promoter engineered to recognize multiple TAL effectors from disparate pathogens.
Proc Natl Acad Sci USA, 106, 20526-20531

Römer P, Strauß T, Hahn S, Scholze H, Morbitzer R, Grau J, Bonas U and Lahaye T (2009)
Recognition of AvrBs3-like proteins is mediated by specific binding to promoters of matching pepper Bs3 alleles.
Plant Physiol, 150, 1697-1712
 

2008

Schornack S, Minsavage GV, Stall RE, Jones JB and Lahaye, T (2008)
Characterization of AvrHah1 a novel AvrBs3-like effector from Xanthomonas gardneri with virulence and avirulence activity.
New Phytol., 179, 546-556
 

2007

Römer P, Hahn S, Jordan T, Strauß T, Bonas U and Lahaye T (2007)
Plant-pathogen recognition mediated by promoter activation of the pepper Bs3 resistance gene.
Science, 318, 645-648

Anand A, Krichevsky A, Schornack S, Lahaye T, Tzfira T, Tang Y, Citovsky V and Mysore KS (2007)
Arabidopsis VIRE2 INTERACTING PROTEIN2 is required for Agrobacterium T-DNA integration in plants.
Plant Cell, 19, 1695-1708
 

2006

Schornack S, Meyer A, Römer P, Jordan T and Lahaye T (2006)
Gene-for-gene mediated recognition of nuclear-targeted AvrBs3-like bacterial effector proteins.
J Plant Physiol, 163, 256-272

Jordan T, Römer P, Meyer A, Szczesny R, Pierre M, Piffanelli P, Bendahmane A, Bonas U and Lahaye T (2006)
Physical delimitation of the pepper Bs3 resistance gene specifying recognition of the AvrBs3 protein from Xanthomonas campestris pv. vesicatoria.
Theor Appl Genet, 113, 895-905

Bhat RA, Lahaye T and Panstruga R (2006)
The visible touch: in planta visualization of protein-protein interactions by fluorophore-based methods.
Plant Methods, 2, 1-12
 

2005

Schornack S, Peter K, Bonas U and Lahaye T (2005)
Expression levels of avrBs3-like genes affect recognition specificity in tomato Bs4 but not in pepper Bs3 mediated perception.
Mol Plant-Microbe Interact, 18, 1215-1225

Peterhänsel C and Lahaye T (2005)
Be fruitful and multiply: gene amplification inducing pathogen resistance.
Trends Plant Sci, 10, 257-260

Freialdenhoven A, Orme J, Lahaye T and Schulze-Lefert P (2005)
Barley Rom1 reveals a potential link between race-specific and nonhost resistance responses to powdery mildew fungi.
Mol Plant-Microbe Interact, 18, 291-299
 

2004

Schornack S, Ballvora A, Gürlebeck D, Peart J, Baulcombe D, Baker B, Ganal M, Bonas U and Lahaye T (2004)
The tomato resistance protein Bs4 is a predicted non-nuclear TIR-NB-LRR protein that mediates defense responses to severely truncated derivatives of AvrBs4 and overexpressed AvrBs3.
Plant J, 37, 46-60

Lahaye T (2004)
Illuminating the molecular basis of gene-for-gene resistance; Arabidopsis thaliana RRS1-R and its interaction with Ralstonia solanacearum popP2.
Trends Plant Sci, 9, 1-4
 

2003

Von Roepenack-Lahaye E, Newman M-A, Schornack S, Hammond-Kosack KE, Lahaye T, Jones JDG, Daniels MJ and Dow JM (2003)
p-Coumaroylnoradrenaline, a novel plant metabolite implicated in tomato defense against pathogens.
J Biol Chem, 278, 43373-43383
 

2002

Lahaye T (2002)
The Arabidopsis RRS1-R disease resistance gene - uncovering the plant's nucleus as new battlefield of plant defense?
Trends Plant Sci, 7, 425-427

Jordan T, Schornack S and Lahaye T (2002)
Alternative splicing of transcripts encoding Toll-like plant resistance proteins – what is the functional relevance to innate immunity?
Trends Plant Sci, 7, 392-398

Bonas U and Lahaye T (2002)
Plant disease resistance triggered by pathogen-derived molecules: refined models of specific recognition.
Curr Opin Microbiol, 5, 44-50
 

2001

Lahaye T and Bonas U (2001)
Molecular secrets of bacterial type III effector proteins.
Trends Plant Sci, 6, 479-485

Collins NC, Lahaye T, Peterhänsel C, Freialdenhoven A, Corbitt M and Schulze-Lefert P (2001)
Sequence haplotypes revealed by sequence-tagged site fine mapping of the Ror1 gene in the centromeric region of barley chromosome 1H1[w].
Plant Physiol, 125, 1236–1247

Ballvora A, Schornack S, Baker BJ, Ganal M, Bonas U and Lahaye T (2001)
Chromosome landing at the tomato Bs4 locus.
Mol Genet Genomics, 266, 639-645

Ballvora A, Pierre M, Van den Ackerveken G, Schornack S, Rossier O, Ganal M, Lahaye T and Bonas U (2001)
Genetic mapping and functional analysis of the tomato Bs4 locus, governing recognition of the Xanthomonas campestris pv. vesicatoria AvrBs4 protein.
Mol Plant-Microbe Interact, 14, 629-638
 

2000

Shirasu K, Schulman AH, Lahaye T and Schulze-Lefert P (2000)
A contiguous 66-kb barley DNA sequence provides evidence for reversible genome expansion.
Genome Res, 10, 908-915

Pierre M, Noël L, Lahaye T, Ballvora A, Veuskens J, Ganal M and Bonas U (2000)
High-resolution genetic mapping of the pepper resistance locus Bs3 governing recognition of the Xanthomonas campestris pv. vesicatora AvrBs3 protein.
Theor Appl Genet, 113, 895-905

Bassie L, Noury M, Lepri O, Lahaye T, Christou P and Capell T (2000)
Promoter strength influences polyamine metabolism and morphogenic capacity in transgenic rice tissues expressing the oat adc cDNA constitutively.
Transgenic Res, 9, 33-42

Shirasu K, Lahaye T, Tan M-W, Zhou F, Azevedo C and Schulze-Lefert, P (1999)
A novel class of eukaryotic zinc-binding proteins is required for disease resistance signaling in barley and development in C. elegans.
Cell, 99, 355-366
 

1999

Graner A, Lahaye T and Keller B (1999)
Plant genomics and the genetics of disease resistance.
Trends Genet, 15, 391-392
 

1998

Lahaye T, Shirasu K and Schulze-Lefert P (1998)
Chromosome landing at the barley Rar1 locus.
Mol Gen Genet, 260, 92-101

Lahaye T, Hartman S, Töpsch S, Freialdenhoven A, Yano M and Schulze-Lefert (1998)
High-resolution genetic and physical mapping of the Rar1 locus in barley.
Theor Appl Genet, 97, 526-534
 

1996

Lahaye T, Rueger B, Toepsch S, Thalhammer J and Schulze-Lefert P (1996)
Detection of single-copy sequences with Digoxigenin-labeled probes in a complex plant genome after separation on pulsed-field gels.
Biotechniques, 21, 1067-1072