Institute of Evolution and Ecology (EvE)

Electron microscopy

SEM

Scanning electron microscopy

3D - Reconstruction

 of serial sections and MicroCT data 

TEM

Transmission electron microscopy

Our electron microscopy laboratory, which is integrated into the Network for Electron Microscopy Tübingen (NET), not only serves the research and teaching purposes of our own group, but also acts as a service location for other working groups at the Biology Department.

Our members of staff give advice concerning the various sample preparation techniques and the use of our microscopes. They also prepare samples and support external users of our scanning electron microscope. In general, the use of our apparatus is free for members of the Department of Biology, but we have to charge appropriate operating costs for our machines (according to the agreement below) for large projects (>20 samples).

For external users (outside of the Department of Biology), samples can be analysed with our transmission and scanning electron microscopes for a corresponding fee (see below).

The following traditional preparation techniques and microscopes are available at our laboratory:

Preparation technologies

  • Chemical fixation and contrasting
  • Ultramicrotomy (including serial-sectioning techniques)
  • Hand microtome sectioning
  • Critical-Point-Drying: Polaron 3000, Polaron 3100
  • Sputtercoating: Emitech K550X
  • Cryo-Transfer-Device: EMITECH K1250X

 Equipment:

  • One scanning electron microscope (Zeiss Evo LS10)
  • Two transmission electron microscopes (Philips Tecnai10, Zeiss 109)
  • Two ultramicrotomes (Leica Reichert Ultracut S and UCT)

Contact:  Monika Meinert (SEM) and Julia Straube (TEM)

Fees for external users (price list valid since 01.05.2014, all fees plus VAT):

Users of Universität Tübingen

Transmission electron microscopy (independent use after briefing)

50 Euro / h

 

Scanning electron microscopy (use only by a member of our group)

40 Euro /h plus 10 - 15 Euro /h for proportional

operating costs and material

 

Cryo-Applications: 50 Euro /h plus 10 - 15 Euro /h

for proportional operating costs and material

External institutions, companies and private persons (commissioned work):

Scanning electron microscopy (use only by a member of our group)

80 Euro /h plus 10 - 15 Euro /h for proportional

operating costs and material
 

Cryo-Applications: 90 Euro /h plus 10 - 15 Euro /h

for proportional operating costs and material