Algorithms in Bioinformatics

SplitsTree App

Software for computing phylogenetic networks

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SplitsTree App is an all-new implementation of the old SplitsTree4 program that was published in 2006.

The new SplitsTree App is designed as an open source project that provides a wide range of algorithms and processes for computing and working with both unrooted and rooted phylogenetic trees and networks. Much work has been invested in accommodating much larger datasets (more taxa, longer sequences, more trees, improved algorithms) than its predecessor programs, SplitsTree4 and Dendroscope3, and its user interface is based on the JavaFX library, giving it a modern look and feel.

A paper describing the new program and extensive user documentation are near completion and will be submitted for publication in April 2024.

Download the SplitsTree App here:  Download

SplitsTree4 is a widely used application for computing unrooted phylogenetic networks from molecular sequence data. Given an alignment of sequences, a distance matrix or a set of trees, the program will compute a phylogenetic tree or network using methods such as split decomposition, neighbor-net, consensus network, super networks methods or methods for computing hybridization or simple recombination networks. Download the old SplitsTree4 program here: Download

If you use SplitsTree in your work, then please cite the following paper: D. H. Huson and D. Bryant, Application of Phylogenetic Networks in Evolutionary Studies, Mol. Biol. Evol., 23(2):254-267, 2006.

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Download the latest version of the program here.