Publications
2020
Examining histone modification crosstalk using immobilized libraries established from ligation-ready nucleosomes. Chem. Sci. 11 (2020); 9218-9225
: The Arabidopsis Nα -acetyltransferase NAA60 locates to the plasma membrane and is vital for the high salt stress response. New Phytol. 228 (2020); 554-569
Bienvenut W. V., Brünje A., Boyer J.-B., Mühlenbeck J. S., Bernal G., Lassowskat I., Dian C., Linster E., Dinh T. V., Koskela M. M., Jung V., Seidel J., Schyrba L. K., Ivanauskaite A., Eirich J., Hell R., Schwarzer D., Mulo P., Wirtz M., Meinnel T., Giglione C., Finkemeier I.: Dual lysine and N‐terminal acetyltransferases reveal the complexity underpinning protein acetylation. Mol Syst Biol. 16 (2020); e9464
: Asymmetric Disulfanylbenzamides as Irreversible and Selective Inhibitors of Staphylococcus aureus Sortase A. ChemMedChem 15 (2020); 839-850
2019
Seidel J., Meisinger T., Sindlinger J., Pieloch P., Finkemeier I., Schwarzer D.: Peptide-based 2-aminophenylamide probes for targeting endogenous class I HDAC complexes. Chembiochem 20 (2019); 3001-3005
2018
Hagmann V., Sommer S., Fabian P., Bierlmeier J., van Treel N., Mootz H. D., Schwarzer D., Azevedo J. E., Dodt G.: Chemically monoubiquitinated PEX5 binds to the components of the peroxisomal docking and export machinery. Sci. Rep. 8 (2018); 16014
Ernst C., Heidrich J., Sessler C, Sindlinger J., Schwarzer D., Koch P., and Boeckler F. M.: Switching Between Bicyclic and Linear Peptides - The Sulfhydryl-Specific Linker TPSMB Enables Reversible Cyclization of Peptides. Front. Chem. 6 (2018); 484
Ernst C., Sindlinger J., Schwarzer D., Koch P., and Boeckler F. M.: The Symmetric Tetravalent Sulfhydryl-Specific Linker NATBA Facilitates a Combinatorial “Tool Kit” Strategy for Phage Display-Based Selection of Functionalized Bicyclic Peptides. ACS Omega 3 (2018); 12361-12368
Fischle W., Schwarzer D.: Chemistry meets epigenetics: from chromatin tools to small molecules affecting the readout of our genomes. Curr. Opin. Chem. Biol. 45 (2018); vii-ix
Koskela M. M., Brünje A., Ivanauskaite A., Grabsztunowicz M., Lassowskat I., Neumann U., Dinh T. V., Sindlinger J., Schwarzer D., Wirtz M., Tyystjärvi E., Finkemeier I., Mulo P.: Chloroplast Acetyltransferase NSI Is Required for State Transitions in Arabidopsis thaliana. Plant Cell 30 (2018); 1695-1709
Füßl M., Lassowskat I., Née G., Koskela M. M., Brünje A., Tilak P., Giese J., Leister D., Mulo P., Schwarzer D., Finkemeier I.: Beyond Histones: New Substrate Proteins of Lysine Deacetylases in Arabidopsis Nuclei. Front. Plant Sci. 9 (2018); 461
2017
Hartl M., Füßl M., Boersema P. J., Jost J.-O., Kramer K., Bakirbas A., Sindlinger J., Plöchinger M., Leister D., Uhrig G., Moorhead G. G. B., Cox J., Salvucci M. E., Schwarzer D., Mann M., Finkemeier I.: Lysine acetylome profiling uncovers novel histone deacetylase substrate proteins in Arabidopsis. Mol. Syst. Biol. 13 (2017); 949
Schmohl L., Bierlmeier J., von Kügelgen N., Kurz L., Reis P., Barthels F., Mach P., Schutkowski M., Freund C., Schwarzer D.: Identification of sortase substrates by specificity profiling. Bioorg Med Chem. 25 (2017); 5002-5007
Schmohl L., Bierlmeier J., Gerth F., Freund C., Schwarzer D.: Engineering sortase A by screening a second-generation library using phage display. J Pept Sci. 23 (2017); 631-635
2016
Liokatis S., Klingberg R., Tan S., Schwarzer D.: Differentially Isotope-Labeled Nucleosomes to Study Asymmetric Histone Modification Crosstalk by Time-Resolved NMR Spectroscopy. Angew Chem Int Ed Engl. 55 (2016); 8262-5
Hiragami-Hamada K., Soeroes S., Nikolov M., Wilkins B., Kreuz S., Chen C., De La Rosa-Velázquez I.A., Zenn H.M., Kost N., Pohl W., Chernev A., Schwarzer D., Jenuwein T., Lorincz M., Zimmermann B., Walla P.J., Neumann H., Baubec T., Urlaub H., Fischle W.: Dynamic and flexible H3K9me3 bridging via HP1b dimerization establishes a plastic state of condensed chromatin. Nat Commun. 7 (2016); 11310
Sindlinger J., Bierlmeier J., Geiger L.C., Kramer K., Finkemeier I., Schwarzer D.: Probing the structure-activity relationship of endogenous histone deacetylase complexes with immobilized peptide-inhibitors. J Pept Sci. 22 (2016); 352-9
Fischle W., Schwarzer D.: Probing Chromatin-modifying Enzymes with Chemical Tools. ACS Chemical Biology 11 (2016) 689-705; review
Stützer A., Liokatis S., Kiesel A., Schwarzer D., Sprangers R., Söding J., Selenko P., Fischle W.: Modulations of DNA Contacts by Linker Histones and Post-translational Modifications Determine the Mobility and Modifiybility of Nucleosomal H3 Tails. Molecular Cell 61 (2016) 247–259
Seidel J., Klockenbusch C., Schwarzer D.: Investigating deformylase and deacylase activity of mammalian and bacterial sirtuins. Chembiochem 17 (2016) 398-402
Dose A., Sindlinger J., Bierlmeier J., Bakirbas A., Schulze-Osthoff K., Einsele-Scholz S., Hartl M., Essmann F., Finkemeier I., Schwarzer D.: Interrogating Substrate Selectivity and Comaprison of Endogenous Histone Deacetylase Complexes with Chemical Probes. Angew Chem Int Ed Engl. 55 (2016) 1192-1195
2015
Fischle W., Mootz H., Schwarzer D.: Synthetic histone code. Curr Opin Chem Biol. 28 (2015) 131-140; Review
Jost O., Hanswillemenke A., Schwarzer D.: A miniaturized readout strategy for endogenous histone deacetylase activity. Mol Biosyst. 11 (2015) 1820-1823
Thongwichian R., Kosten J., Benary U., Rose H.M., Stuiver M., Theillet F.X., Dose A., Koch B., Yokoyama H., Schwarzer D., Wolf J., Selenko P.: A Multiplexed NMR-Reporter Approach to Measure Cellular Kinase and Phosphatase Activities in Real-Time. J Am Chem Soc. 137 (2015) 6468-6471
Fischle W., Schwarzer D., Mootz H.D.: Chemical biology: Chromatin chemistry goes cellular. Nat Chem. 5 (2015) 371-373; Review
Schmohl L., Wagner F. R., Schümann M., Krause E., Schwarzer D.: Semisynthesis and Initial Characterization of Sortase A Mutants Containing Selenocysteine and Homocysteine. Bioorg. Med Chem. 15 (2015) 2883-2889
Klingberg R., Jost J.O., Schümann M., Gelato K.A., Fischle W., Krause E., Schwarzer D.: Analysis of phosphorylation-dependent protein-protein interactions of histone H3. ACS Chem Biol. 10 (2015) 138-145
2014
Schmohl L., Schwarzer D.: Sortase-mediated ligations for the site-specific modification of proteins. Curr Opin Chem Biol. 22 (2014) 122-128; Review
Abel S., Geltinger B., Heinrich N., Michl D., Klose A., Beyermann M., Schwarzer D.: Semisynthesis and optimization of G protein-coupled receptor mimics. J Pept Sci. 20 (2014) 831-836
Gelato K.A., Tauber M., Ong M.S., Winter S., Hiragami-Hamada K., Sindlinger J., Lemak A., Bultsma Y., Houliston S., Schwarzer D., Divecha N., Arrowsmith C.H., Fischle W.: Accessibility of Different Histone H3-Binding Domains of UHRF1 Is Allosterically Regulated by Phosphatidylinositol 5-Phosphate. Mol Cell. 54 (2014) 905-919
Schmohl L., Schwarzer D.: Chemo-enzymatic three-fragment assembly of semisynthetic proteins. J Pept Sci. 20 (2014) 145-151
2013
Lang D., Schümann M., Gelato K., Fischle W., Schwarzer D., Krause E.: Probing the acetylation code of histone H4. Proteomics 13 (2013) 2989-2997
Teschke T., Geltinger B., Dose A., Freund C., Schwarzer D.: Probing the recognition of post-translational modifications by combining sortase-mediated ligation and phage-assisted selection. ACS Chem Biol. 8 (2013) 1692-1697
Finkemeier I., Schwarzer D.: Lysin-Acetylierung: eine altbekannte Proteinmodifikation in neuem Licht. BIOspektrum. 7 (2013) 810–812; Review
2012
Dose A., Jost J.O., Spieß A.C., Henklein P., Beyermann M., Schwarzer D.: Facile synthesis of colorimetric histone deacetylase substrates. Chem Commun. 48 (2012) 9525-9527
Munari F., Soeroes S., Zenn H.M., Schomburg A., Kost N., Schroeder S., Klingberg R., Rezaei-Ghaleh N., Stuetzer A., Gelato K.A., Walla P.J., Becker S., Schwarzer D., Zimmermann B., Fischle W., Zweckstetter M.: Methylation of K9 in histone H3 directs alternative modes of highly dynamic interaction of heterochromatin protein hHP1β with the nucleosome. J Biol Chem. 287 (2012) 33756-33765
Liokatis S., Stützer A., Elsässer S.J., Theillet F.-X., Klingberg R., van Rossum B., Schwarzer D., Allis C.D., Fischle W., Selenko P.: Phosphorylation of histone H3 Ser10 establishes a hierarchy for subsequent intramolecular modification events. Nat Struct Mol Biol. 19 (2012) 819-823
Bekei B., Rose H.M., Herzig M., Dose A., Schwarzer D., Selenko P.: In-cell NMR in Mammalian cells: part 1. Methods Mol Biol. 895 (2012) 43-54
Schwarzer D.: Die Chemische Biologie beschreitet neue Wege in der Chromatinforschung. BIOspektrum. 3 (2012) 248–250; Review
Theillet F.X., Liokatis S., Jost J.O., Bekei B., Rose H.M., Binolfi A., Schwarzer D., Selenko P.: Site-specific mapping and time-resolved monitoring of lysine methylation by high-resolution NMR spectroscopy. J Am Chem Soc. 134 (2012) 7616-7619
Lebrun P., Raze D., Fritzinger B., Wieruszeski J.M., Biet F., Dose A., Carpentier M., Schwarzer D., Allain F., Lippens G., Locht C.: Differential contribution of the repeats to heparin binding of HBHA, a major adhesin of Mycobacterium tuberculosis. PLoS One. 7 (2012) e32421
Broncel M., Krause E., Schwarzer D., Hackenberger C.P.: The Alzheimer's Disease Related Tau Protein as a New Target for Chemical Protein Engineering. Chemistry. 18 (2012) 2488-2492
Schwarzer D., Ludwig C., Thiel I.V., Mootz H.D.: Probing intein-catalyzed thioester formation by unnatural amino acid substitutions in the active site. Biochemistry. 51 (2012) 233-242
Seeliger D., Soeroes S., Klingberg R., Schwarzer D., Grubmüller H., Fischle W.: Quantitative Assessment of Protein Interaction with Methyl-Lysine Analogues by Hybrid Computational and Experimental Approaches. ACS Chem Biol. 7 (2012) 150-154
2011
Piotukh K., Geltinger B., Heinrich N., Gerth F., Beyermann M., Freund C., Schwarzer D.: Directed evolution of sortase A mutants with altered substrate selectivity profiles. J Am Chem Soc. 133 (2011) 17536-17539
Stojic L., Jasencakova Z., Prezioso C., Stutzer A., Bodega B., Pasini D., Klingberg R., Mozzetta C., Margueron R., Puri P.L., Schwarzer D., Helin K., Fischle W., Orlando V.: Chromatin regulated interchange between polycomb repressive complex 2 (PRC2)-Ezh2 and PRC2-Ezh1 complexes controls myogenin activation in skeletal muscle cells. Epigenetics Chromatin. 4 (2011) 16
Dose A., Liokatis S., Theillet F.X., Selenko P., Schwarzer D.: NMR profiling of histone deacetylase and acetyl-transferase activities in real time. ACS Chem Biol. 20 (2011) 419-424
2010
Tsai W.W., Wang Z., Yiu T.T., Akdemir K.C., Xia W., Winter S., Tsai C.Y., Shi X., Schwarzer D., Plunkett W., Aronow B., Gozani O., Fischle W., Hung M.C., Patel D.J, Barton M.C.: TRIM24 links a noncanonical histone signature to breast cancer. Nature. 468 (2010) 927-932
Liokatis S., Dose A., Schwarzer D., Selenko P.: Simultaneous Detection of Protein Phosphorylation and Acetylation by High-Resolution NMR Spectroscopy. J Am Chem Soc. 132 (2010) 14704-14705
Schwarzer D.: Chemical tools in chromatin research. J Pept Sci. 16 (2010) 530–537; Review
Hackenberger C.P.R., Arndt H.D., Schwarzer D.: Semisynthese: Werkzeug für die Chemische Biologie. Chemie in Unserer Zeit. 44 (2010) 198-206; Review
Arndt H.D, Hackenberger C.P.R., Schwarzer D.: Semisynthese: Chemie mit den Molekülen der Natur. Chemie in Unserer Zeit. 44 (2010) 130-137; Review
2009
Ludwig C., Schwarzer D., Zettler J., Garbe D., Janning P., Czeslik C., Mootz H.D.: Semisynthesis of proteins using split inteins. Methods Enzymol. 462 (2009) 77-96; Review
Schwarzer D.: Hacking the genetic code of mammalian cells. Chembiochem. 10 (2009) 1602-1604; Review
2008
Hackenberger C.P.R., Schwarzer D.: Chemoselective Ligation and Modification Strategies for Peptides and Proteins. Angew Chem Int Ed. 47 (2008) 10030-10074; Review
Ludwig C., Schwarzer D., Mootz H.D.: Interaction studies and alanine scanning analysis of a semi-synthetic split intein reveal thiazoline ring formation from an intermediate of the protein splicing reaction. J Biol Chem. 283 (2008) 25264-25272
2007
Mootz H.D., Schwarzer D.: Biochemie und Molekular Biologie 2006. Nachrichten aus der Chemie. 55 (2007) 276-279; Review
2006
Schwarzer D., Zhang Z., Zheng W., Cole P.A.: Negative Regulation of a Protein Tyrosine Phosphatase by Tyrosine Phosphorylation. J Am Chem Soc. 128 (2006) 4192-4193
2005
Schwarzer D., Cole P.A.: Protein semisynthesis and expressed protein ligation: chasing a protein's tail. Curr Opin Chem Biol. 9 (2005) 561-569; Review
Shen K., Hines A.C., Schwarzer D., Pickin K.A., Cole P.A.: Protein kinase structure and function analysis with chemical tools. Biochim Biophys Acta. Proteins and Proteomics. 1754 (2005) 65-78; Review
Zheng W., Schwarzer D., Lebeau A., Weller J.L., Klein D.C., Cole P.A.: Cellular stability of serotonin N-acetyltransferase conferred by phosphonodifluoromethylene alanine (Pfa) substitution for Ser-205. J Biol Chem. 280 (2005) 10462-10467
2004
Linne U., Schwarzer D., Schroeder G., Marahiel M.A.: Mutational analysis of a type II thioesterase associated with nonribosomal peptide synthesis. Eur J Biochem. 271 (2004) 1536-1545
2003
Richardt A., Kemme T., Wagner S., Schwarzer D., Marahiel M.A., Hovemann B.T.: Ebony: a novel nonribosomal peptide synthetase for beta-alanine conjugation with biogenic amines in Drosophila. J Biol Chem. 278 (2003) 41160-41166
Schwarzer D., Finking R., Marahiel M.A.: Nonribosomal Peptides: From Genes to Products. Nat Prod Rep. 20 (2003) 275-287; Review
2002
Schwarzer D., Mootz H.D., Linne U., Marahiel M.A.: Regeneration of misprimed nonribosomal peptide synthetases by type II thioesterases. Proc Natl Acad Sci. U.S.A. 99 (2002) 14083-14089
Mootz H.D., Kessler N., Linne U., Eppelmann K., Schwarzer D., Marahiel M.A.: Decreasing the ring-size of a nonribosomal peptide antibiotic by in frame module deletion in the biosynthetic genes. J Am Chem Soc. 124 (2002) 10980-10981
Mootz H.D., Schwarzer D., Marahiel M.A.: Ways of assembling complex natural products on modular nonribosomal peptide synthetases. ChemBioChem. 3 (2002) 490-504; Review
Bruner S.D., Weber T., Kohli R.M., Schwarzer D., Marahiel M.A., Walsh C.T., Stubbs M.T.: Structural basis for the cyclization of the lipopeptide antibiotic surfactin by the thioesterase domain SrfTE. Structure. 10 (2002) 301-310
2001
Schwarzer D., Mootz H.D., Marahiel M.A.: Exploring the impact of different thioesterase domains for the design of hybrid peptide synthetases. Chem Biol. 8 (2001) 997-1010
Kohli R.M., Trauger J.W., Schwarzer D., Marahiel M.A., Walsh C.T.: Generality of peptide cyclization catalyzed by isolated thioesterase domains of nonribosomal peptide synthetases. Biochemistry. 40 (2001) 7099-7108
Schwarzer D., Marahiel M.A.: Multimodular biocatalysts for natural product assembly. Naturwissenschaften 88 (2001) 93-101; Review
2000
Mootz H.D., Schwarzer D., Marahiel M.A.: Construction of hybrid peptide synthetases by module and domain fusions. Proc Natl Acad Sci. U.S. A. 97 (2000) 5848-5853
Book Chapters
Hackenberger C.R.P., Bode J.W., Schwarzer D.: Chemoselective Peptide Ligation: A Privileged Tool for Protein Synthesis. (Chapter 13) Amino Acids, Peptides and Proteins in Organic Chemistry, Wiley-VCH, Andrew B. Huges (ed.) (2011) 445-494
Schwarzer D.: Histone Acetylation. Encyclopedia of Molecular Pharmacology, 2nd Ed., Springer Berlin Heidelberg (2008) 592-595