Salmonella uses type III secretion systems (T3SS) to export proteins of diverse functions to manipulate the behavior of eukaryotic host cells in order to allow its invasion or colonization. Understanding the assembly of T3SS can facilitate further anti-infective drug development.
Thus in my work, I am studying the coordination of assembly of the inner and outer membrane parts of the T3SS encoded on Salmonella pathogenicity island 1 and I also want to know at which stage of this assembly the secretion starts and the needle assembles.
For that, I am using bacterial genetics and molecular biology as well as membrane protein biochemistry and protein-protein interaction analysis.