Uni-Tübingen

P8: cGMP and visual signal processing – NO/cGMP pathway dynamics and the role of amacrine cells

Aims

To gain insights into the role of amacrine cells in NO-cGMP mediated neuromodulation of inner retinal signal processing. Specifically, to better our understanding of the dynamic regulation of NO release and NO-cGMP pathway activation in the context of neural activity in the light-adapted retina.

Questions and Methods

NO/cGMP pathways in the retina

St. Louis Internship

Williams Lab

The Williams lab in St. Louis (USA) adds critical expertise to the project, which will enable us to consider potential effects of the experimental approach – in vitro vs. in vivo – on the observed NO-mediated changes in retinal signal processing. Hence, the doctoral students will be trained in in vivo retinal imaging. Moreover, the Williams lab’s focus on the impact of metabolic and signaling pathways on RGC degeneration will allow the doctoral students to consider their results also in the context of retinal diseases.

St. Louis Co-mentor

Assistant Prof. Philip Williams, PhD

Link to Williams lab 

Doctoral Students

Dominic Gonschorek (graduated in December 2023)

Dominic Gonschorek obtained his B.Sc. in Biology at the Free University of Berlin focusing on Neurobiology and Biomedicine. He continued his studies by earning a Master's degree in Neuroscience at the Carl-von-Ossietzky University of Oldenburg where he primarily focused on neurosensory systems, experimental and computational Neuroscience. This combination led Dominic to pursue his doctoral studies in the lab of Thomas Euler. Here, he investigates the role of cGMP pathways for visual signal processing in bipolar cells of the mouse retina.

Tom Schwerd-Kleine

Tom Schwerd-Kleine obtained his B.Sc. in Integrated Life Science at the Friedrich-Alexander University in Erlangen, Germany. There, he gained a broad knowledge about research in the life sciences. For his bachelor’s thesis he joined the lab of Prof. Dr. J.H. Brandstätter, where he characterised phenotypical consequences in the retina of a conditional knockout mouse line. This thesis sparked his interest in neuroscience research which led Tom to the Technical University of Munich, where he studied Biomedical Neuroscience. In his master’s thesis he joined the lab of Prof. Dr. T. Misgeld with the aim to establish a genetically encoded fluorescent sensor to measure intracellular ATP concentrations in the model of larval zebrafish in vivo. To combine the knowledge which he gained both in Erlangen and Munich, Tom decided to pursue his PhD in the lab of Thomas Euler. The aim of his project will be to elucidate potential neuromodular influences of cGMP on signal processing in the mouse retina.

Florentyna Deja (associated PhD student)

Florentyna Deja acquired her B.Sc. in Biology at the University of Tübingen, where she got particularly interested in vision, systems neuroscience and experimental and analytical approaches to study brain function. For her master’s degree, she continued her studies at the University of Tübingen in Neuroscience. During her master thesis in the Euler lab, she used two-photon microscopy to investigate how light stimulus intensity and excitation laser of the microscope affect the responses of ganglion cells in the mouse retina. She started her doctoral studies in the same lab, where she continued studying retinal signal processing at both the photoreceptor and the ganglion cell level.


Key Publications

Qiu Y, Klindt DA, Szatko KP, Gonschorek D, Hoefling L, Schubert T, Busse L, Bethge M, Euler T. Efficient coding of natural scenes improves neural system identification. bioRxiv 2022. doi:10.1101/2022.01.10.475663.  

Behrens C, Yadav SC, Korympidou MM, Zhang Y, Haverkamp S, Irsen S, Schaedler A, Lu X, Liu Z, Lause J, St-Pierre F, Franke K, Vlasits A, Dedek K, Smith RG, Euler T, Berens P, Schubert T. 2022. Retinal horizontal cells use different synaptic sites for global feedforward and local feedback signaling. Curr Biol 32(3):545-558.e5. doi:10.1016/j.cub.2021.11.055

Das S, Popp V, Power M, Groeneveld K, Yan J, Melle C, Rogerson L, Achury M, Schwede F, Strasser T, Euler T, Paquet-Durand F, Nache V. 2022. Redefining the role of Ca(2+)-permeable channels in photoreceptor degeneration using diltiazem. Cell Death Dis 13:47. doi:10.1038/s41419-021-04482-1.

Gonschorek D, Hoefling L, Szatko KP, Franke K, Schubert T, Dunn B, Berens P, Klindt DA, Euler T. Removing inter-experimental variability from functional data in systems neuroscience. NeurIPS 2021, Spotlight doi:10.1101/2021.10.29.466492.

Strauss S, Korympidou MM, Ran Y, Franke K, Schubert T, Baden T, Berens P, Euler T, Vlasits AL. Center-surround interactions underlie bipolar cell motion sensitivity in the mouse retina. Nat Commun 2022, 13, 5574, 10.1038/s41467-022-32762-7