Subproject B09: Regulatory impact of 14-3-3 proteins on NPH3-like protein function
Principal investigator:
Prof. Dr. Claudia Oecking
University of Tuebingen
ZMBP, Plant Physiology
Auf der Morgenstelle 32
72076 Tübingen
+49 7071 29 76679
claudia.oecking@zmbp.uni-tuebingen.de
Summary:
NON PHOTOTROPIC HYPOCOTYL 3 (NPH3) is a key component of the blue light-triggered phototropic response, acting upstream of auxin redistribution. We could show that light-regulated complex formation with 14-3-3 proteins mediates cycling of NPH3 between the plasma membrane and the cytosol - a process essential for NPH3 function. This project seeks to uncover the molecular determinants and mechanisms underlying NPH3 function in the context of its oligomerization, spatially distinct interaction partners and phosphorylation status. In addition, the impact of NPH3 on the organization of PIN auxin efflux carriers in subdomains of the plasma membrane will be explored.