Sub-project A04: The molecular mechanism of kinesin-12 motor proteins in the positioning of the cell division plane
Priciple Investigator
Dr. Sabine Müller
Universität Tübingen
ZMBP, Entwicklungsgenetik
Auf der Morgenstelle 3, 72076 Tübingen
Tel 07071 - 297 8888
Fax 07071 - 297 5797
sabine.mueller@zmbp.uni.tuebingen.de
Prof. Dr. Erik Schäffer
Universität Tübingen
ZMBP, Cellular Nanoscience
Auf der Morgenstelle 28, 72076 Tübingen
Tel 07071 - 29 72076
Fax 07071 - 29 5042
erik.schaeffer@zmbp.uni-tuebingen.de
Summary
Project A04 will perform in vivo and in vitro analysis of kinesin 12 (POK) motor proteins during the positioning and establishment of the plane of cell division. POKs are relevant for the maintenance and recognition of the plane of cell division. Using optical tweezers, modern quantitative single-molecule force-measurements will contribute to elucidate the yet unknown molecular mechanism of these specific plant kinesins in vitro. Furthermore, for life cell imaging, a light-sheet microscope will be constructed to investigate single-molecules (kinesin) in living plant cells.