Sub-project D02: In planta analysis of a plasma membrane-associated brassinosteroid response pathway
Principle Investigator
Prof. Dr. Klaus Harter
Universität Tübingen
ZMBP, Pflanzenphysiologie
Auf der Morgenstelle 1, 72076 Tübingen
Tel 07071 – 29 72605
Fax 07071 – 29 3287
klaus.harter@zmbp.uni-tuebingen.de
PD Dr. Frank Schleifenbaum
Universität Tübingen
IPTC, Biophysikalische Chemie
Auf der Morgenstelle 18, 72076 Tübingen
Tel 07071 – 29 76222
FAX 07071 – 29 5490
frank.schleifenbaum@uni-tuebingen.de
Prof. Dr. Ursula Kummer
Universität Heidelberg
COS, Bioquant
INF 267, 69120 Heidelberg
Tel 06221-54-51278
Fax 06221-54-51483
ursula.kummer@bioquant.uni-heidelberg.de
Summary
The hormone-induced and gene expression-independent cell wall expansion and cell elongation growth is initiated by the activation of the P-type H+-ATPases of the plasma membrane. In the project D02 we would like to study, how in coaction with which cofactors and by which post-translational modification the plasma membrane-intrinsic brassinoide receptor BRI1 is able to trigger the P-type H+-ATPases with respect to cell wall expansion and elongation growth. Furthermore, we would like to mathematically model this signaling process to obtain information about its regulatory crucial steps and dynamics. In addition, we would like to establish and to improve super-resolution microscopy (STED, PALM/STORM) in combination with spectroscopy (Nano-FRET) for the detection and analysis of BRI1-containing protein complexes in the plasma membrane.